LOCUS       sequence-491927-        8529 bp    DNA     circular SYN 05-NOV-2025
DEFINITION  Expression vector for mouse Pgc-1alpha with mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Chandra R, Engeln M, Francis TC, Konkalmatt P, Patel D, Lobo MK
  TITLE     A Role for Peroxisome Proliferator-Activated Receptor Gamma 
            Coactivator-1alpha in Nucleus Accumbens Neuron Subtypes in Cocaine 
            Action.
  JOURNAL   Biol Psychiatry. 2017 Apr 1;81(7):564-572. doi: 
            10.1016/j.biopsych.2016.10.024. Epub 2016 Oct 28.
  PUBMED    27939396
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.biopsych.2016.10.024"; journalName: "Biol Psychiatry"; 
            date: "2017-04-1- 1"; volume: "81"; issue: "7"; pages: "564-572"
COMMENT     Sequence Label: pAAV-hSyn-DIO-PGC-1alpha-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..8529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..298
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(376..409)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(460..493)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    2919..3492
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3086..3103)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3313..3332
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      3493..3502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3499..3501,3502..3504,3505..4215)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3544..3565)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3805..3824)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4152..4173
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4230..4263
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4314..4347
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(4385..4832)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     repeat_region   4877..5006
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(5068..5656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5148..5167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5827..6618,6619..6687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6450..6469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6688..6792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6860..6878
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6916..6938)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7038..7057
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7074..7529)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7211..7232)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7423..7442
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   7604..7744
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7615..7744
                     /label=AAV2 ITR
     polyA_signal    7784..8260
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     8042..8061
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    join(8292..8529,1..351)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8398..8409
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
       61 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
      121 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
      181 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
      241 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
      301 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tatcgataag
      361 cttgatatcg aattcataac ttcgtatagc atacattata cgaagttatt ctttgcacca
      421 ttctaaagaa taacagtgat aatttctggg ttaaggcaaa taacttcgta taggatactt
      481 tatacgaagt tatgctagta tggcttggga catgtgcagc caagactctg tatggagtga
      541 catagagtgt gctgctctgg ttggtgagga ccagcctctt tgcccagatc ttcctgaact
      601 tgacctttct gaacttgatg tgaatgactt ggatacagac agctttctgg gtggattgaa
      661 gtggtgtagc gaccaatcgg aaatcatatc caaccagtac aacaatgagc ctgcgaacat
      721 atttgagaag atagatgaag agaatgaggc aaacttgcta gcggtcctca cagagacact
      781 ggacagtctc cccgtggatg aagacggatt gccctcattt gatgcactga cagatggagc
      841 cgtgaccact gacaacgagg ccagtccttc ctccatgcct gacggcaccc ctccccctca
      901 ggaggcagaa gagccgtctc tacttaagaa gctcttactg gcaccagcca acactcagct
      961 cagctacaat gaatgcagcg gtcttagcac tcagaaccat gcagcaaacc acacccacag
     1021 gatcagaaca aaccctgcca ttgttaagac cgagaattca tggagcaata aagcgaagag
     1081 catttgtcaa cagcaaaagc cacaaagacg tccctgctca gagcttctca agtatctgac
     1141 cacaaacgat gaccctcctc acaccaaacc cacagaaaac aggaacagca gcagagacaa
     1201 atgtgcttcc aaaaagaagt cccatacaca accgcagtcg caacatgctc aagccaaacc
     1261 aacaacttta tctcttcctc tgaccccaga gtcaccaaat gaccccaagg gttccccatt
     1321 tgaaaacaag actattgagc gaaccttaag tgtggaactc tctggaactg caggcctaac
     1381 tcctcccaca actcctcctc ataaagccaa ccaagataac cctttcaagg cttcgccaaa
     1441 gctgaagccc tcttgcaaga ccgtggtgcc accgccaacc aagagggccc ggtacagtga
     1501 gtgttctggt accaaaggca gccactccac caagaaaggg cccgagcaat ctgagttgta
     1561 cgcacaactc agcaagtcct cagggctcag ccgaggacac gaggaaagga agactaaacg
     1621 gcccagtctc cggctgtttg gtgaccatga ctactgtcag tcactcaatt ccaaaacgga
     1681 tatactcatt aacatatcac aggagctcca agactctaga caactagact tcaaagatgc
     1741 ctcctgtgac tggcaggggc acatctgttc ttccacagat tcaggccagt gctacctgag
     1801 agagactttg gaggccagca agcaggtctc tccttgcagc accagaaaac agctccaaga
     1861 ccaggaaatc cgagcggagc tgaacaagca cttcggtcat ccctgtcaag ctgtgtttga
     1921 cgacaaatca gacaagacca gtgaactaag ggatggcgac ttcagtaatg aacaattctc
     1981 caaactacct gtgtttataa attcaggact agccatggat ggcctatttg atgacagtga
     2041 agatgaaagt gataaactga gctacccttg ggatggcacg cagccctatt cattgttcga
     2101 tgtgtcgcct tcttgctctt cctttaactc tccgtgtcga gactcagtgt caccaccgaa
     2161 atccttattt tctcaaagac cccaaaggat gcgctctcgt tcaagatcct tttctcgaca
     2221 caggtcgtgt tcccgatcac catattccag gtcaagatca aggtccccag gcagtagatc
     2281 ctcttcaaga tcctgttact actatgaatc aagccactac agacaccgca cacaccgcaa
     2341 ttctcccttg tatgtgagat cacgttcaag gtcaccctac agccgtaggc ccaggtacga
     2401 cagctatgaa gcctatgagc acgaaaggct caagagggat gaataccgca aagagcacga
     2461 gaagcgggag tctgaaaggg ccaaacagag agagaggcag aagcagaaag caattgaaga
     2521 gcgccgtgtg atttacgttg gtaaaatcag acctgacaca acgcggacag aattgagaga
     2581 ccgctttgaa gtttttggtg aaattgagga atgcaccgta aatctgcggg atgatggaga
     2641 cagctatggt ttcatcacct accgttacac ctgtgacgct ttcgctgctc ttgagaatgg
     2701 atatacttta cgcaggtcga acgaaactga cttcgagctg tacttttgtg gacggaagca
     2761 atttttcaag tctaactatg cagacctaga taccaactca gacgattttg accctgcttc
     2821 caccaagagc aagtatgact ctctggattt tgatagttta ctgaaggaag ctcagagaag
     2881 cttgcgcagg taagatcttg taggcggccg cgatatcgcc cctctccctc ccccccccct
     2941 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3001 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3061 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3121 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3181 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3241 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3301 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3361 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3421 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3481 acacgatgat aagccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3541 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3601 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3661 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
     3721 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3781 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3841 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3901 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3961 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4021 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4081 ctgctgcccg acaaccacta cctgagctac cagtccaaac tgagcaaaga ccccaacgag
     4141 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4201 gacgagctgt acaagtgata gggcgcgcca taacttcgta taatgtatgc tatacgaagt
     4261 tattaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc tgcataactt
     4321 cgtataaagt atcctatacg aagttattcc ggagtcgact ctagaggatc cggtaccttc
     4381 tcgactgcgc tctcaggcac gacacgactc ctccgctgcc caccgcagac tgaggcagcg
     4441 ctgagtcgcc ggcgccgcag cgcagatggt cgcgcccgtg cccccctatc tcgcgcctcg
     4501 cgtggtgcgg tccggctggg ccggcggcgg cgcggacgcg accaaggtgg ccgggaaggg
     4561 gagtttgcgg gggaccggcg agtgacgtca gcgcgccttc agtgctgagg cggcggtggc
     4621 gcgcgccgcc aggcgggggc gaaggcactg tccgcggtgc tgaagctggc agtgcgcacg
     4681 cgcctcgccg catcctgttt cccctccccc tctctgatag gggatgcgca atttggggaa
     4741 tgggggttgg gtgcttgtcc agtgggtcgg ggtcggtcgt caggtaggca cccccacccc
     4801 gcctcatcct ggtcctaaaa cccacttgca ctcatacgca gggccctctg cagtctagac
     4861 acacgcgtgc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4921 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggcgg cctcagtgag
     4981 cgagcgagcg cgcagctgcc tgcaggacat gtgagcaaaa ggccagcaaa aggccaggaa
     5041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6841 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6901 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6961 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7021 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc ataaaattgt
     7081 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     7141 ccaatagacc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagagtt
     7201 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     7261 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag
     7321 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     7381 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     7441 agcgggcgct aaggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7501 cgcgcttaat gcgccgctac agggcgcgta ctatggttgc tttgacgtat gcggtgtgaa
     7561 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gcccctgcag gcagctgcgc
     7621 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
     7681 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
     7741 tcctgcggcc gctcggtccg cacgtggtta cctacaaaat cagaaggaca gggaagggag
     7801 cagtggttca cgcctgtaat cccagcaatt tgggaggcca aggtgggtag atcacctgag
     7861 attaggagtt ggagaccagc ctggccaata tggtgaaacc ccgtctctac caaaaaaaca
     7921 aaaattagct gagcctggtc atgcatgcct ggaatcccaa caactcggga ggctgaggca
     7981 ggagaatcgc ttgaacccag gaggcggaga ttgcagtgag ccaagattgt gccactgcac
     8041 tccagcttgg ttcccaatag accccgcagg ccctacaggt tgtcttccca acttgcccct
     8101 tgctccatac cacccccctc caccccataa tattatagaa ggacacctag tcagacaaaa
     8161 tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca acttccaggg
     8221 ccaggagagg cactggggag gggtcacagg gatgccaccc gtagatctct cgagcagcgc
     8281 tcggtatcga tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg
     8341 aagacgcgga agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt
     8401 gagggccgaa gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacataggc
     8461 gagcagccaa ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa
     8521 cagccgagc
//
