LOCUS       sequence-491936-        6806 bp    DNA     circular SYN 05-NOV-2025
DEFINITION  5' AAVLINK plasmid for MYO5C expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-HA-5'-MYO5C
FEATURES             Location/Qualifiers
     source          1..6806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     repeat_region   4069..4209
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4069..4198
                     /label=AAV2 ITR
     rep_origin      4284..4739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4371..4390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4581..4602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4756..4775)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4875..4897
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4935..4953)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5021..5125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5126..5194,5195..5986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5344..5363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6157..6745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6646..6665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctgc ggtggccgag ctgtacacgc agtacaacag
      781 ggtctggatt cccgatcctg aagaagtttg gaagtctgct gaaatagcca aggactacag
      841 agttggtgac aaggtcctgc gactcctgct ggaggatgga acggagctgg attattctgt
      901 caatccagaa tctctgcctc cacttcggaa tcctgacatc ctcgtgggcg agaatgacct
      961 cacggctctc agctatcttc acgagcccgc ggtgctccac aacctcagaa tccgctttgc
     1021 agaatccaaa ctcatttaca cctacagtgg aatcattttg gtggccatga atccttacaa
     1081 gcagttgcca atatacggag atgccatcat ccacgcctac agcgggcaga acatgggcga
     1141 tatggaccca cacatatttg ccgtggcaga agaggcatac aagcagatgg ccagaaacaa
     1201 cagaaaccag tccataattg taagtgggga gtcaggtgct ggaaagacag tgtcggctcg
     1261 ctatgccatg aggtactttg ccaccgtcag caaatcgggc agcaacgctc acgtggaaga
     1321 caaggtcctg gcatccaatc ccatcaccga ggccgttgga aatgccaaga ccacccgcaa
     1381 tgacaatagt agtcggtttg ggaaatacac agaaatcagt tttgatgaac aaaatcaaat
     1441 tataggagcc aacatgagca cttacctcct ggagaaatcc agagttgtct ttcaatcgga
     1501 aaatgaacga aattaccaca ttttctatca gctttgtgca tctgcacagc agtcggaatt
     1561 taaacatctt aaattgggga gtgccgaaga atttaattat acaagaatgg gaggcaatac
     1621 tgtcattgag ggtgtgaatg atcgagctga aatggtagag actcaaaaga ccttcacgct
     1681 tctgggtttc aaggaggatt ttcagatgga cgtttttaaa atcctggcag ccatcctaca
     1741 tctgggcaac gtgcagatca ccgcggtggg caacgagagg tcctcagtta gtgaggatga
     1801 cagtcacctg aaggtgttct gtgagctcct gggcctggag agtggcagag ttgctcagtg
     1861 gctgtgcaat cgcaaaatcg tcacaagctc tgagacggtg gtaaaaccca tgaccaggcc
     1921 tcaggctgtc aacgccaggg atgcactggc caaaaagatc tatgctcacc tgttcgactt
     1981 cattgtggag agaattaacc aagcgttgca gttttcaggc aagcagcaca cttttattgg
     2041 tgttttggac atttatggtt ttgaaacctt tgatgtgaac agctttgaac aattttgcat
     2101 caattacgct aatgaaaaac tgcaacaaca gtttaacatg catgtcttca aactggaaca
     2161 agaagaatac atgaaggaag atataccttg gacgctgata gatttttatg acaatcaacc
     2221 agttattgac ctgattgaag caaaaatggg aattctggag ttactggatg aagaatgttt
     2281 gttaccacat ggaactgatg aaaactggct tcaaaagctg tataataatt ttgtcaacag
     2341 gaaccctttg tttgaaaagc ctagaatgtc aaacacatcc tttgtcatcc agcactttgc
     2401 tgataaggta gagtataaat gtgaaggttt cctggagaaa aacagagaca ccgtctatga
     2461 catgctggtt gaaatcctga gagcaagcaa gtttcatctc tgtgccaact tttttcaaga
     2521 aaatccaact cctccttctc cttttggttc aatgattaca gttaaatctg caaagcaagt
     2581 catcaagcca aacagcaagc atttccgaac cacagttggg agcaagttcc gcagctctct
     2641 gtacttgctc atggagaccc tcaatgcgac gacgccccac tacgttcgat gcatcaagcc
     2701 aaatgatgag aagttaccct ttgaatttga ctccaaaaga attgttcagc agctgcgagc
     2761 ctgcggcgtt ttagaaacga ttcgcattag tgcacagagc tacccttcca ggtggacata
     2821 catcgagttc tacagtcgct acggcattct catgaccaag caagagcttt ccttcagcga
     2881 taaaaaggag gtgtgcaagg tggttttaca cagactcatc caggattcta atcagtacca
     2941 gtttggtaaa accaaaattt tcttcagagc aggacaagtg gcttatttag agaaacttcg
     3001 attggataaa ctgaggcaga gttgtgttat ggtacaaaag cacatgcgtg gctggctcca
     3061 gaggaaaaaa ttcctccgag agagacgagc cgccctgata atccagcagt acttccgggg
     3121 tcagcaaact gtgaggaaag ctattactgc agtggcctta aaagaagctt gggcagccat
     3181 aatcattcag aagcactgcc gtgggtatct tgttcgcagc ctgtatcagt tgattcgcat
     3241 ggccaccatc acaatgcagg cctacagccg aggattcctg gcaaggagga ggtatcgaaa
     3301 gatgctggag gaacataagg ctgtgatcct acagaaatac gcacgggcgt ggctggccag
     3361 acgcagattc cagagtatcc gacgattcgt gcttaatatt cagcttactt acagggtcca
     3421 gcgtttgcag aaaaagctgg aagatcagaa caaagaaaac catgggctgg tggagaagct
     3481 gactagcctg gctgctcttc gagctgggga tgtggaaaag attcagaagc tggaagcaga
     3541 actagaaaaa gcagccactc acaggcgaaa ttacgaggag aaggggaaga gatacaggga
     3601 tgctgtggaa gagaaattgg caaagcttca gaagcataat tcagaactgg aaacacagaa
     3661 agaacaaata cagctgaagc ttcaagagaa gactgaagag ttaaaagaaa aaatggacaa
     3721 cctcaccaag cagctctttg atgatgtaca aaaggaagaa cggcaaagaa tgcttcttga
     3781 aaaaagtttt gaactgaaaa cacaagacta tgagaagcag attcagtctt tgaaagaaga
     3841 aattaaagct ctcaaggatg agaagatgca actccaacac ctggtggagg gggagcacgt
     3901 cacttctgat ggcttgaagg cggaagtggc ccgcctgagc aagcaggtaa gtatcaaggt
     3961 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
     4021 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
     4081 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4141 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4201 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4261 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     4381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4441 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     4501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     4621 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     4681 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     4741 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4801 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4861 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4921 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4981 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5041 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5101 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5161 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5221 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5281 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5341 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5401 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5461 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5521 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5581 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5641 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5701 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5761 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5821 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5881 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5941 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6001 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6061 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6781 cttttgctgg ccttttgctc acatgt
//
