LOCUS       sequence-491987-        9291 bp    DNA     circular SYN 05-NOV-2025
DEFINITION  ATF6 - Unfolded protein response element (UPRE) gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE2
FEATURES             Location/Qualifiers
     source          1..9291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2121..2152
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2179..2188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2185..3834
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3838..3957
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4049..4637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4102..4122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4520..4531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4652..4968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4819..4954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5019..5021,5022..5024,5025..5738)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5064..5085)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5325..5344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5672..5693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5810..6043
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6115..6236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6152..6171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6206..6225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6276..6411
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6431..6450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6432..6450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6460..6477)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6460..6476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6469..6491)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6618..7073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6705..6724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6915..6936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7099..7203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7204..7272,7273..8064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7422..7441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8235..8823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8724..8743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8977..8994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9111..9132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9147..9152,9153..9170,9171..9177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9185..9201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9190..9212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9209..9225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9209..9225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9244..9264
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9246..9264
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcgcac cagcgtgtgg atcctcgaga caggtgctga cgtggcattc ctcgagacag
     1981 gtgctgacgt ggcattcctc gagacaggtg ctgacgtggc attcctcgag acaggtgctg
     2041 acgtggcatt cctcgagaca ggtgctgacg tggcattcga tccgagaaca gatctggcct
     2101 cggcggccaa gcttagacac tagagggtat ataatggaag ctcgacttcc agcttggcaa
     2161 tccggtactg ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg
     2221 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc
     2281 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     2341 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     2401 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg
     2461 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc
     2521 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg
     2581 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg
     2641 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg
     2701 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc
     2761 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac
     2821 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc
     2881 cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg
     2941 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta
     3001 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt
     3061 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc
     3121 gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac
     3181 ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc
     3241 cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag
     3301 gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc
     3361 cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc
     3421 gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc
     3481 ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc
     3541 gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg
     3601 cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc
     3661 atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg
     3721 cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc
     3781 cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgaattct
     3841 cacggcttcc ctcccgaggt ggaggagcag gccgccggca ccctgcccat gagctgcgcc
     3901 caggagagcg gcatggatag acaccctgct gcttgcgcca gcgccaggat caacgtctaa
     3961 ggccgcgact ctagaacgcg tcactgcggc ttgccggggc ggtgactcga ctacagacat
     4021 cagcgcgtac cggttagtaa tgatcgacaa tcaacctctg gattacaaaa tttgtgaaag
     4081 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4141 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4201 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4261 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4321 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4381 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4441 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4501 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     4561 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     4621 ttgggccgcc tccccgcctg gcgatggtac cggtgtggaa agtccccagg ctccccagca
     4681 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4741 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4801 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4861 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     4921 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4981 gcttgtatat ccattttcgg atctgatcag cacctagcat ggtgagcaag ggcgaggagc
     5041 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5101 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5161 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg
     5221 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5281 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     5341 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5401 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     5461 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     5521 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     5581 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     5641 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     5701 ccgggatcac tctcggcatg gacgagctgt acaagtaagg tacctttaag accaatgact
     5761 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5821 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5881 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5941 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6001 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     6061 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6241 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6421 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6481 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6541 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6601 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7021 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7081 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7141 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7201 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7261 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7321 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7381 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7441 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7501 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7561 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7621 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7681 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7741 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7801 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7861 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7921 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7981 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8041 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8101 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8341 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8941 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9001 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9061 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9181 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9241 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta a
//
