LOCUS       sequence-491988-        9205 bp    DNA     circular SYN 05-NOV-2025
DEFINITION  XBP1 - ER stress gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE20
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2035..2066
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2093..2102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2099..3748
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3752..3871
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3963..4551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4016..4036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4434..4445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4566..4882
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4733..4868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4933..4935,4936..4938,4939..5652)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4978..4999)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5239..5258)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5586..5607
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5724..5957
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6029..6150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6066..6085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6120..6139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6190..6325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6345..6364)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6346..6364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6374..6391)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6374..6390)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6383..6405)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6532..6987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6619..6638)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6829..6850
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7013..7117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7118..7186,7187..7978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7336..7355)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8149..8737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8638..8657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8891..8908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9025..9046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9061..9066,9067..9084,9085..9091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9099..9115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9104..9126
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9123..9139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9123..9139
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9158..9178
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9160..9178
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcgcac cagcgtgtgg atccaacaga tgcgtcatgc gtcatgcgtc atgcgtcatg
     1981 cgtcatctca aagatccgag aacagatctg gcctcggcgg ccaagcttag acactagagg
     2041 gtatataatg gaagctcgac ttccagcttg gcaatccggt actgttggta aagccaccat
     2101 ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac
     2161 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
     2221 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
     2281 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
     2341 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
     2401 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
     2461 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
     2521 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
     2581 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
     2641 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
     2701 taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca
     2761 tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt
     2821 gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg
     2881 ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa
     2941 gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat
     3001 cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa
     3061 ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg
     3121 cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc
     3181 agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac
     3241 actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta
     3301 cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg
     3361 cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct
     3421 gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca
     3481 ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc
     3541 cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta
     3601 tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga
     3661 ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa
     3721 ggccaagaag ggcggcaaga tcgccgtgaa ttctcacggc ttccctcccg aggtggagga
     3781 gcaggccgcc ggcaccctgc ccatgagctg cgcccaggag agcggcatgg atagacaccc
     3841 tgctgcttgc gccagcgcca ggatcaacgt ctaaggccgc gactctagaa cgcgtcactg
     3901 cggctttata ccgtaaacag acgactacag acatcagcgc gtaccggtta gtaatgatcg
     3961 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4021 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4081 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4141 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4201 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4261 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4321 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     4381 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4441 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4501 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggcgatg
     4561 gtaccggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4621 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4681 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4741 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4801 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4861 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     4921 tcagcaccta gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4981 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     5041 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     5101 ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc ccgctacccc
     5161 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     5221 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     5281 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     5341 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     5401 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     5461 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5521 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     5581 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     5641 ctgtacaagt aaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5701 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5761 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5941 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6001 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6121 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6301 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6361 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6421 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6481 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6961 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7021 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7081 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7141 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7201 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7261 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7321 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7381 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7441 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7501 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7561 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7621 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7681 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7741 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7801 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7861 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7921 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7981 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8041 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8101 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8161 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8221 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8281 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8341 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8401 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8461 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8521 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8581 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8641 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8701 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8761 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8821 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8881 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8941 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9001 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9061 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9121 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9181 acaaaagctg gagctgcaag cttaa
//
