LOCUS       sequence-492082-        3578 bp    DNA     circular SYN 17-NOV-2025
DEFINITION  AAV expression of Cre recombinase-activated jRCaMP1b, a red 
            fluorescent calcium sensor protein, from the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G,
            Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens 
            MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS
  TITLE     Sensitive red protein calcium indicators for imaging neural 
            activity.
  JOURNAL   eLife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727.
  PUBMED    27011354
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2016-03-24"; volume: "5. pii: e12727. doi"; pages: " 
            10.7554/eLife.12727"
COMMENT     Sequence Label: pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40
FEATURES             Location/Qualifiers
     source          1..3578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     673..695
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     757..776
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    811..844
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    907..940
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(2190..2204,2205..2213))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(2217..2249)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(2253..2270)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2353..2386)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(2457..2490)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2532..3120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2585..2605)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3003..3014)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3163..3284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3174..3193)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3228..3247
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3446..3575
                     /label=AAV2 ITR
ORIGIN
        1 ttctccgtgt ctttgtggcc gggcgcctca gatttgtaaa agagacaggt acatggcctt
       61 tggggttttt cctccttggc cgtacatcta cgttttagtc ctcccttttt ccatgggcgg
      121 gggggccccc cccccccccc cccccccccc cccccccccc cccccccccc ccccaatttt
      181 tttttttttt tttttttaat ttttttgggg gggggggggg gggggggggg ggggggggcg
      241 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      301 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      361 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      421 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      481 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
      541 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
      601 tttgtgcggg gggagcggct cggggctgtc cgcgggggga cggctgcctt cgggggggac
      661 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
      721 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
      781 ctcatcattt tggcaaagaa ttggatcccc ataacttcgt ataaagtatc ctatacgaag
      841 ttatatcaaa ataggaagac caatgcttca ccatcgaccc gaattgccaa gcatcaccat
      901 cgacccataa cttcgtataa tgtatgctat acgaagttat actagagctt tcacttcgct
      961 gtcatcattt gtacaaactc ttcgtagttt acctgaccat ccccatcgct gtctgcttcc
     1021 ctgatcattt catcaacctc ttcatctgtt aacttctctc caaggtttgt catcacgtgg
     1081 cgaagctctg ctgcactgat gtagccattg ccatccttat caaacacgcc gaacgcttct
     1141 ctaatttctt cttcactgtc tgtgtatttc attttgcctg ccatcataat caggaactca
     1201 gggaagtcga ttgtgccgtc accgtcggca tctacttcat tgatcatgtc ctgcagctct
     1261 gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc ccatctcctt ggttgttatt
     1321 gtcccatccc cgtccttgtc aaatagggag aaagcctctt taaattctgc gatctgctct
     1381 tcagtcagtt gggagtccgt aggctcccaa cctttggttt tcttttgcat tacagcacca
     1441 ttgctcggga agttcacacc gcgaacctgc acgtgataca ccaggcagcc atcctccaga
     1501 gacgtgtctt gcataacggt aattacgccg ccgtcttcgt aacgggtcac acgttcccag
     1561 gtaaaacctt ccgggaagga ctgcttaaag aaatccggga tgcctttcgg atatttgatg
     1621 aaggtacggg agccatacat aaaggacgtc gccaggatgt cgaacgcaaa cggcagcgga
     1681 ccaccctcga tcactttgat acgcatagtc tgggtgccca tatacgggtt gccttcgcct
     1741 tcaccagtgc atttgaactg gtggccgttt acggagcctt ccagaaccac tttcatacgc
     1801 atgttctcct tgatcaggga gttcatgctc cctccggtac cgccgccacc acccaggccc
     1861 acaaacttgg caactgcgtg ttcacgctgt acgacaaaca tttcgttatc gctctcctcc
     1921 aggcgctcca gacggtggct gacgtaatga atggcaggca tcttaatgtt gccgacagtc
     1981 tttttggagc ggtaggtggt cacgaaagaa caggacaggt gaccgccgcc atcaactttc
     2041 agcgccatgt gagtgtaacc acgcagacca ccatccgctg ggtacatcat ttcggtgttg
     2101 ttcgctgagc tcagccgacc tatagctctg actgcgtgac cccacttatt ccacttacgt
     2161 cgcgatgagt cgaccattgt tgcgagatcc ttatcgtcat cgtcgtacag atcccgaccc
     2221 atttgctgtc caccagtcat gctagccata ccatgatgat gatgatgatg agaacctcct
     2281 ccagtcttgt taatatcaag tccagccaac ttaagagcaa gctcgttctg cagcatggtg
     2341 gcggatccta gtataacttc gtataggata ctttatacga agttatcatt gggattcttc
     2401 ctattttgat ccaagcatca ccatcgaccc tctagtccag atctcaccat cgacccataa
     2461 cttcgtatag catacattat acgaagttat gtccctcgaa gaggttcgaa ttcgatatca
     2521 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2581 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2641 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2701 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2761 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2821 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2881 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2941 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3001 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3061 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3121 atcgataccg tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt
     3181 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3241 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3301 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     3361 tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag
     3421 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     3481 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     3541 gcccgggcgg cctcagtgag cgagcgagcg cgcagctg
//
