LOCUS       sequence-492095-        9030 bp    DNA     circular SYN 06-NOV-2025
DEFINITION  Recombinant protein expression of PCMTD1(1-231).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Warmack RA, Pang EZ, Peluso E, Lowenson JD, Ong JY, Torres JZ, 
            Clarke SG
  TITLE     Human Protein-l-isoaspartate O-Methyltransferase Domain-Containing 
            Protein 1 (PCMTD1) Associates with Cullin-RING Ligase Proteins.
  JOURNAL   Biochemistry. 2022 May 17;61(10):879-894. doi: 
            10.1021/acs.biochem.2c00130. Epub 2022 Apr 29.
  PUBMED    35486881
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.2c00130"; journalName: "Biochemistry"; date: 
            "2022-05-17- 17"; volume: "61"; issue: "10"; pages: "879-894"
COMMENT     Sequence Label: pMAPLe4 PCMTD1(1-231)
FEATURES             Location/Qualifiers
     source          1..9030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1950..1968
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1989..2007,2008..2013,2014..2030,2031..2036,
                     2037..2062)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1989..2007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2014..2032
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2072..2080
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2086..2796
                     /codon_start=1
                     /product="tobacco vein mottling virus NIa protease 
                     (Nallamsetty et al., 2004)"
                     /label=TVMV protease
                     /translation="MSKALLKGVRDFNPISACVCLLENSSDGHSERLFGIGFGPYIIAN
                     QHLFRRNNGELTIKTMHGEFKVKNSTQLQMKPVEGRDIIVIKMAKDFPPFPQKLKFRQP
                     TIKDRVCMVSTNFQQKSVSSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIIDGN
                     ILGIHSLTHTTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDAPE
                     DDFMAKKTVAAIMD"
     terminator      2829..2915
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3041..4123)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4085..4104
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4124..4201)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4407..4426
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4510..4529
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4510..4528
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4529..4553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4568..4590
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4597..5697
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNDKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      5554..5563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5671..5694
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5707..5727
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             5728..5745
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5755..5775
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      6528..6575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             6642..6662
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             6663..6680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6690..6710
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      7463..7510
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7547..8002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7634..7653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7844..7865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8094..8909)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8094..8909)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8817..8836
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8901..8920
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggctaa gatagtcgac gtctaagaaa
     1921 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     1981 acctcgagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     2041 acatcagcag gacgcactga ccgaattgat ttaaggaggt aacatatgtc taaagctttg
     2101 ctgaagggcg tgcgcgattt taatccgatc tctgcttgcg tatgcctgct ggaaaactcc
     2161 tcggatggtc atagtgaacg tctgtttggc attggttttg gcccgtatat cattgccaac
     2221 cagcatctgt ttcgtcgtaa caatggcgaa ctgaccatca aaaccatgca tggtgaattc
     2281 aaagtcaaaa actctaccca gctgcagatg aaaccggttg aaggccgtga cattatcgtt
     2341 atcaaaatgg ctaaagactt cccgccgttc ccgcagaaac tgaaattccg tcagccgacc
     2401 atcaaagatc gtgtgtgcat ggtgtccacc aactttcagc agaaaagcgt ctcgagcctg
     2461 gtgtctgaat cctctcacat tgtgcataaa gaagacactt ctttctggca gcactggatc
     2521 accactaaag atggccagtg tggcagccca ctagtttcca tcattgatgg caacattctg
     2581 ggcatccaca gcctgactca taccaccaac ggtagcaact acttcgtgga atttccggaa
     2641 aaattcgtgg cgacttatct agatgccgcg gatggttggt gcaaaaactg gaaattcaac
     2701 gcggataaaa tcagctgggg ttcctttacc ctggttgaag atgcgccgga agatgacttc
     2761 atggccaaaa aaactgttgc cgccatcatg gactaataaa gatcccatgg tacgcgtgct
     2821 agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2881 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagtag tcatgccccg
     2941 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     3001 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3061 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3121 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     3181 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     3241 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     3301 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     3361 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     3421 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     3481 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     3541 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     3601 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     3661 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     3721 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     3781 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     3841 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     3901 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     3961 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     4021 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     4081 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     4141 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     4201 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     4261 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     4321 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     4381 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     4441 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     4501 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     4561 aaataatttt gtttaacttt aagaaggaga tataccatga aaatcgaaga aggtaaactg
     4621 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
     4681 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
     4741 ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt
     4801 ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
     4861 aagctgtatc cgtttacctg ggatgccgta cgttacaacg acaagctgat tgcttacccg
     4921 atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa
     4981 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
     5041 atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat
     5101 gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc
     5161 gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac
     5221 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac
     5281 ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg
     5341 ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac
     5401 gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat
     5461 gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac
     5521 gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc ccagaaaggt
     5581 gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
     5641 atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactaat
     5701 ggctcagaaa cagttcggtt ccagtctcat caccatcacc atcactcgag ctcagagaac
     5761 ctgtatttcc agagtatggg aggagctgtg agtgctgggg aagataatga tgacttaatt
     5821 gataatttaa aagaagctca gtatattcgt actgaaagag tggagcaagc cttcagagcg
     5881 attgatcgtg gagattacta tttggaaggc tacagagaca atgcttacaa agacttagcc
     5941 tggaagcatg gaaacatcca cttgtcagca ccttgcattt attctgaagt tatggaagca
     6001 ttgaaacttc aaccaggatt gtcttttctt aacctgggaa gtggaaccgg atatttaagt
     6061 acaatggtgg gcttaatttt aggtcctttt ggaataaatc atgggattga gcttcattca
     6121 gatgtggtgg aatatgccaa ggaaaaactg gagagcttca tcaaaaatag tgatagcttt
     6181 gataaatttg agttctgtga acctgcattt gttgttggta attgcctcca gatagcttct
     6241 gacagtcatc agtatgatcg aatttattgt ggagctggag tacagaaaga ccatgaaaac
     6301 tacatgaaaa tattactaaa agttggaggc atattagtca tgcctataga ggatcagtta
     6361 acacagatta tgcgaactgg acagaacact tgggaaagta aaaatatcct tgctgtttca
     6421 tttgctccac ttgtgcaacc aagtaagaat gataatggca aaccagatta actgctaaca
     6481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     6541 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     6601 tggcgaatgg gacgcgccag taaggcaacc ctaatggctc agaaacagtt cggttccagt
     6661 ctcatcacca tcaccatcac tcgagctcag agaacctgta tttccagagt atgggaggag
     6721 ctgtgagtgc tggggaagat aatgatgact taattgataa tttaaaagaa gctcagtata
     6781 ttcgtactga aagagtggag caagccttca gagcgattga tcgtggagat tactatttgg
     6841 aaggctacag agacaatgct tacaaagact tagcctggaa gcatggaaac atccacttgt
     6901 cagcaccttg catttattct gaagttatgg aagcattgaa acttcaacca ggattgtctt
     6961 ttcttaacct gggaagtgga accggatatt taagtacaat ggtgggctta attttaggtc
     7021 cttttggaat aaatcatggg attgagcttc attcagatgt ggtggaatat gccaaggaaa
     7081 aactggagag cttcatcaaa aatagtgata gctttgataa atttgagttc tgtgaacctg
     7141 catttgttgt tggtaattgc ctccagatag cttctgacag tcatcagtat gatcgaattt
     7201 attgtggagc tggagtacag aaagaccatg aaaactacat gaaaatatta ctaaaagttg
     7261 gaggcatatt agtcatgcct atagaggatc agttaacaca gattatgcga actggacaga
     7321 acacttggga aagtaaaaat atccttgctg tttcatttgc tccacttgtg caaccaagta
     7381 agaatgataa tggcaaacca gattaactgc taacaaagcc cgaaaggaag ctgagttggc
     7441 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     7501 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
     7561 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7621 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7681 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7741 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7801 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7861 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7921 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7981 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8041 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     8101 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     8161 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     8221 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     8281 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     8341 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     8401 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     8461 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     8521 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     8581 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     8641 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     8701 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     8761 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     8821 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     8881 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     8941 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9001 agaccccgta gaaaagatca aaggatcttc
//
