LOCUS       sequence-492127-        5035 bp    DNA     circular SYN 06-NOV-2025
DEFINITION  AiE2672m is an enhancer sequence designed to drive AAV-mediated 
            transgene expression in specific population sof brain cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5035)
  TITLE     Basal Ganglia/CERP Enhancer AAVs
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP20004 - pAAV-AiE2672m-minBG-SYFP2-WPRE3-BGHpA
FEATURES             Location/Qualifiers
     source          1..5035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     293..314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(468..487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     587..609
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(647..665)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        733..837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(838..906,907..1698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1056..1075)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1869..2457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2358..2377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2519..2648
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      3565..3574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3571..3573,3574..3576,3577..4287)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3616..3637)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3877..3896)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4224..4245
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4380..4400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4587..4698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4816..4956
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4816..4945
                     /label=AAV2 ITR
     rep_origin      join(5031..5035,1..451)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
       61 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      121 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      181 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
      241 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      301 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
      361 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      421 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
      481 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      541 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      601 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
      661 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      721 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      781 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      841 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      901 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      961 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1021 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1081 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1141 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1201 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1261 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1321 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1381 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1441 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1501 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1561 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1621 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1681 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1741 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1801 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1861 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1921 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1981 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2041 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2101 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2161 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2221 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2281 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2341 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2401 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2461 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc
     2521 tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg cgacctttgg
     2581 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2641 gggttcctgc ggccgcacgc gtggattgta aacatatgcc catcagatcc catttgacag
     2701 gtgtcttttt aaaaggacaa atatcaagct taagctgcta cagaacttaa tcaagtgagt
     2761 agggctgtgt gctacctatt aaccaaggct atctgcctct ggccaatgga agttctgtgt
     2821 tatcttaaaa tcaagccatc tgtcctcctc tgacttccag agtaactttc ttttctcctg
     2881 tctgaagagc cacagctttg gatcctggag aactcagctt cccttgcctc tccagggagt
     2941 ggaagacttt gttttataaa acttcaaatt acaatgcaat atttcgataa ttaaaatact
     3001 ggctgacatt tactgagagc ttactaagtc tcaagcatgg cgcaaagaat gtcttaagac
     3061 aattaaatga gaccatccct tcccagcacc gtggaggact aatggccatt cccattggca
     3121 aagaaactgg ggcttacaga tggctaatac acagctctgg gaagagtgga acttcaactt
     3181 gcaacatttg attctagagt tcatgaccta aaggctgagt atatttgaat gttcgaactg
     3241 gctgggcctc acataggctt cttcaaaacc taaagaaagc aaagtaactg actcagaaga
     3301 aatctgaatc tttcaaacca agggaacaga agcagagttc ctgttgtcct ctacaaccca
     3361 aagcatcaga gtcttcatgg aggatgggtg tgggctgaga cctgagcagc aagcttcatt
     3421 aagatgtctg aagtccctac agccatgtct gcacagagaa gaacgccggc ggagctcggg
     3481 ctgggcataa aagtcagggc agagccatct attgcttaca tttgcttctg ggatccagat
     3541 ctttcgaagc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     3601 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3661 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
     3721 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcgtgcag
     3781 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3841 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3901 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3961 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4021 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     4081 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4141 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
     4201 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4261 actctcggca tggacgagct gtacaagtaa gtcgacggcg cgccgcggcc gcgaattcga
     4321 tatcataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4381 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4441 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
     4501 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4561 aattccgtgg ctcgagagat cttcgactgt gccttctagt tgccagccat ctgttgtttg
     4621 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4681 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4741 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgaga tctcacgtgc
     4801 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4861 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4921 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4981 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcg
//
