LOCUS       sequence-492235-        5609 bp    DNA     circular SYN 07-NOV-2025
DEFINITION  Large terminase from phage ST32.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_ST32
FEATURES             Location/Qualifiers
     source          1..5609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    172..312
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(238..257)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     398..420
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(576..1454)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1481..1765
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1688..1707
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3838..3865
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3884..3975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3976..4044,4045..4836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4194..4213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5007..5595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5496..5515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
       61 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
      181 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
      241 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
      301 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      361 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      421 ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag
      481 cggcatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
      541 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      601 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      661 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      721 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      781 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      841 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      901 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      961 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
     1021 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
     1081 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
     1141 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
     1201 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
     1261 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
     1321 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
     1381 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
     1441 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1501 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1561 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1621 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1681 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1741 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
     1801 gtcaaaaaca gatgacctag gacccgaatt caacgacacg gtggcgagct acctggcaaa
     1861 gcactatggt gatgtcttga aggtcagcag agagcgcttc tgcattctga gcctgccgct
     1921 tctcagacgt tacattagcg aaaacaccgc gatcgttgac agctacgagg agcagatcca
     1981 agcggaactc gagggtcagg gccttgagaa cgatgctgtg ctgcgttccg ttttccgcgc
     2041 tcaactggag cgtctgcgct ccggtgacaa aaaggacagc accgcgtacg cggatgaatt
     2101 gaaaaaggga atccaagcgg gtgttactga ccgcgttgca ggaagcacgc tggaggaacc
     2161 gaactccgaa tatgacaaca ccgatgaatt agttgacaag atcctgcatg atccgcataa
     2221 tgttgaccca acggttattc cggcgaaagt tgcggagcgc gttcgtaaaa tcctgatgaa
     2281 gagcttcgag gcatttggta agtggcagtt tcacatccaa atgggttttc cgtttcaagc
     2341 tcaggatttc cacgatgtga ttttttccgt tggccaaaaa gtggtggacg gcgagatcga
     2401 caggttaatc gtgaccatcc cgcctcgtca ctctaaaacc cagctgatga gcattgcgct
     2461 gccgctgtac tctttctgcc ataatgaaag ctcacataac attattacca gctacgccga
     2521 ggacgtggtt ttggagagca gcggctatat ccgtgccgtg atgatggatc cgttgtttca
     2581 acgtattttt ccgaaagtgc gcattgaccc caacaaacgt tcgctcgagc gttggggtac
     2641 aaccaaagca ggcgtcatgc acgctgtgcc gaccggtggc aaactgaccg gtaaaggcgc
     2701 aggtctgttg gtagccaagt acagcgggtg ctttgttgtt gacgacgtca ttaagccgaa
     2761 agacgcatat tctgataccg ttcgtgcgga gattaacgac cgctttgata acacctttat
     2821 gagccgtctg gcaaatgacg gttgcatcac cgatagctac ggcaaccaga tcaagtgcgc
     2881 acgtaccccg atggttatca tcatgcagcg tgttcacgac caggatctgg tcggttatat
     2941 cttgcgcggt ggctcttctg ataagtacca ttatttgaat attccggcga tcatcgagaa
     3001 gggtgtgggt tcagcggaat ggtatgacaa gctgatcaag aaacagaatt acacccatgc
     3061 catcccgatt ctgtacaacc tgaatcgtaa agaagagaag agcgcgctgt ggccgagtcg
     3121 taagtccttg gagtcactgg aggctatgaa agaaagcaat ccgtacacct ataattccca
     3181 gtatgcgggc gacccatctg cccaaggtca cggcctgatt aaagaggatt ggtggcaaga
     3241 atacgaaccg gatgtgttcg atcgatctcg tatcgtgcgt tcctttatta ctgcggatac
     3301 tgcgagtacc gcgaagagct acagcgatta ttctgtgttg aagcattggg gtgttacgaa
     3361 agagcgcgac gtctattgtc tggatattat gctgggtaaa ttcgaaactc cggaacttaa
     3421 gaccgcgatt atcgacttct ggaataaatg taataaactg gacctgaaat gcgtgggctg
     3481 catcccgcgt gcgctgtaca tggaagacaa gtcggcgggt caattcctga accagcagtt
     3541 catcagagat ggtagcgtac gtgtgttgcc gttgccacgc gacggcatca acggtaacga
     3601 caaggtgacg cgttttttga acgcaatccc ttatttctcc cagaaacgca tcttcttccc
     3661 ggctggccac gaacacattg atcacgttcg tcgtgaaatc ctgggcttca ccagcctggg
     3721 ctccggcacc ggccatgatg actgtgtgga caacgtgagc gacatggttg ccattgaatt
     3781 ttccggtccg agcgccaact acagcgcgtg gatgtaatgc caggcatcaa attaagcaga
     3841 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa
     3901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4201 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     4261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4501 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5581 cggagcctat ggaaaaacgc cagcaacgc
//
