LOCUS       sequence-492297-        4395 bp    DNA     circular SYN 07-NOV-2025
DEFINITION  The plasmid comprises an indicible transcriptional unit encoding 
            Gal4-YY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Caliendo F, Vitu E, Wang J, Kuo SH, Sandt H, Enghuus CN, Tordoff J, 
            Estrada N, Collins JJ, Weiss R
  TITLE     Customizable gene sensing and response without altering endogenous 
            coding sequences.
  JOURNAL   Nat Chem Biol. 2024 Sep 12. doi: 10.1038/s41589-024-01733-y.
  PUBMED    39266721
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Sep 12. doi: 10.1038/s41589-024-01733-y."
COMMENT     Sequence Label: pFC546
FEATURES             Location/Qualifiers
     source          1..4395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(95..886,887..955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     718..737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(956..1060)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1885..1923
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1920..1944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1992..2432
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2439..2459
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2469..2495
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2715..2744
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2718..2747
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     polyA_signal    3421..3469
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3731..4319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3811..3830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
       61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
      241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      961 tcctttttca atattattga agcatttatc aaggttattg tctcatgagc ggatacatat
     1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacgaattgg ccagcgctgc
     1081 attaccctgt tatccctaac cggtttaccg agctcttatt ggttttcaaa cttcattgac
     1141 tgtgccgctc ttcaaccgga gttctgcaat tcatgtgatc tgcgcctggg agctcacggg
     1201 gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag
     1261 cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca
     1321 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct
     1381 tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag ctaggctgaa
     1441 agaggatctt agaatgacac gcgcctggga gctcacgggg acagcccccc cccaaagccc
     1501 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     1561 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     1621 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     1681 cagacacctg gggggatacg gggaaaaagc tggatcataa ctcgacgtgc atactgtccc
     1741 ctccacccca cagtggggac aacttgtgcc gtatcagtcc cctccacccc acagtgggga
     1801 atgccatcag tcgtttggtc ccctccaccc cacagtgggg ttttacccta gtctacaagt
     1861 cccctccacc ccacagtggg gcgaggtagg cgtgtacggt gggaggccta tataagcaga
     1921 gctcgtttag tgaaccgtca gatcgcctgg agaattccag ataagtttgt acaaaaaaga
     1981 gaggtgccac catgaagcta ctgtcctcta tcgaacaagc atgcgatatt tgccgactta
     2041 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
     2101 agtgtcgcta ctctcccaaa accaaaaggt ccccgctgac tagggcacat ctgacagaag
     2161 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaggacc
     2221 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     2281 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     2341 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     2401 gtaacaaagg tcaaagacag ttgactgtat cgatgggccc caagaagaag aggaaggtgg
     2461 gatctggtta cccatacgac gtgccagatt acgccggatc aggttgcatg gcgtctggcg
     2521 acacattgta tatagctaca gatggaagcg aaatgcctgc tgaaatcgta gaactgcatg
     2581 agatcgaggt ggaaaccatc cccgtggaga caatcgaaac aactgtagtg ggggaagaag
     2641 aagaagagga tgacgatgat gaggatggag ggggaggtga tcatggaggt ggaggtggtc
     2701 acggccacgc cgggcaccat catcatcatc accaccacca ccatcatccc ccgatgatcg
     2761 ccctgcagcc actcgtaaca gatgatccta cgcaagtaca tcaccatcag gaagtaatac
     2821 ttgtgcagac gagggaagag gtcgtgggcg gcgacgactc agatggtctt cgcgcggaag
     2881 atggtttcga ggatcaaatt ctcatccccg ttcccgcgcc cgccggcggt gacgacgatt
     2941 atatcgaaca aacccttgtg accgtggctg cggctggcaa gagtggcggt ggtggctcaa
     3001 gcagttccgg tggaggcaga gtcaaaaaag gcggaggaaa aaagtcaggc aaaaagagtt
     3061 acctctccgg gggagctggt gcggccggcg gaggcggagc agatccaggt aataagaaat
     3121 gggaacagaa gcaggtccaa atcaaaaccc tggagggaga gttctcagtt actatgtgga
     3181 gctccgacga gaagaaggac atagaccatg aaaccgttgt cgaggagcaa ataatcggtg
     3241 agaatagccc gcccgactat agcgagtata tgacaggaaa aaaactcccg cccggcggca
     3301 tccccggcat cgacctttct gatcccaaac aacttgctga atttgcaaga atgaagcccc
     3361 ggaagataaa ggaagatgat gcaccacgct gagcttccac tggattgtac aattaccaac
     3421 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgc gctcgctgaa
     3481 gagcggtgcg tttttatgct tgtagtattg tataatgttt ttaagatcct taattaatag
     3541 ggataacagg gtaatatctt gccctgcacg aataccagcg accccttgcc caaatacttg
     3601 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
     3661 ttgtcgccgg catcgttgcg ccacatctag gatctgccag gaaccgtaaa aaggccgcgt
     3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4381 ggattttggt catga
//
