LOCUS       sequence-492312-        2851 bp    DNA     circular SYN 07-NOV-2025
DEFINITION  Level 0 Golden Gate ""T"" module containing the sequence of the 
            terminator of the polyubiquitin 4 gene (LOC111919935) from lettuce 
            (Lactuca sativa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2851)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pL0M-T-tLsUBI
FEATURES             Location/Qualifiers
     source          1..2851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(648..666)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        734..838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1060..1851
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2022..2610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2511..2530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2764..2781
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtctcagctt tggtttggtg gtgttatgaa ggttgttgat gaatgaattt gtgtatgttt
       61 gaatgtttgt gttgtgattg aaactctggt ttgatggttt gaagtgaata attgttgaat
      121 aaaaaaactt tggttttctg tgaaattgat taagccatgt tattttaatt gttcattgtc
      181 tttgtgttgt caaattgcgc atttgaggtt aagtttttgg taacgttttg aacccaacca
      241 aatataatac aattctttat ctaattatcg gataattaaa tatccaattt tcatcaagtt
      301 ggatatgtcc acttataagg catttataac tataatattt tcaattataa atataaatat
      361 ttgtcggttg taattgcaca caaattggat aacagttaga tattttattg aatcaaatct
      421 aacacaataa tcgaaataga tatccattag ataaaactaa accaatttaa tatcctaatt
      481 aaaggttatt tccctatata attcatgagt tcgaagtcat attaatttgc agttttggtc
      541 ttttacatat aatttttgta aatccactat tagttacact ttagttttac tagattatag
      601 cccgcgttcg cttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
      661 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      721 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      781 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      841 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      901 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
      961 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1021 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     1081 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     1141 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     1201 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     1261 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     1321 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1381 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     1441 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     1501 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     1561 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     1621 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     1681 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     1741 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     1801 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     1861 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1921 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2161 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2401 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2641 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2701 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2761 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2821 gcgcgttggc cgattcatta atcactctgt g
//
