LOCUS       sequence-492318-        4137 bp    DNA     circular SYN 07-NOV-2025
DEFINITION  Level 0 Golden Gate ""PU"" module containing the domesticated 
            sequence of the promoter of the polyubiquitin 4 gene (LOC111919935) 
            from lettuce (Lactuca sativa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4137)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pL0M-PU-pLsUBI
FEATURES             Location/Qualifiers
     source          1..4137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1931..1949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2017..2121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2343..3134
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3305..3893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3794..3813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4047..4064
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaggagcgg tttttcagtg acctctctaa agtgatatat tcccgacaaa atggttattt
       61 tacaaaaaaa ataatcattt tttttcaaat tattcattta tatgggttag aatactcaat
      121 ttccctgtgt tatttaattt aaaatcattt ccaatataca aacatacttt attatctgat
      181 tatttataat aaattttcat aaagtttcac ctaatctttt attccttata ttataaaatt
      241 tatccatcca aatcaaactt attttgtata atttaaaagt attttcaata taatcatttg
      301 gtttgtggtg acgtagtaaa acatatgaaa tatatgatgt ataacttatt gatcaatgtt
      361 caaattttga cactactcgg tcctgtggcc ataaatgttt gtcggcattg actccaaaac
      421 atgaagtatc ctctttaata aattaaagtt tttttttgtc acttgtcaca ctctcatgca
      481 ttatacaaaa tgtcattttg tcataatttt gagatattta tttttcattt gtcatttact
      541 tcacttttta tttttgaata tatccttaat ttagttttta taaattaacc atatcataat
      601 aactatatta attttaaatt ttgaatttca aacttcaatc gaaactttaa ataaatttac
      661 aattgaaacg tttgtattta acattttgtt ataattagac atttgataca cgagtcagat
      721 aactaaacac ataattttaa tttatttttt tcattttttt tttataattc ttacttattc
      781 caaatttcaa attcaaataa atttgctgtt taatcattcg tatttaacat tttattgtaa
      841 tcaacccgta taatatacaa gtatcacaac tactaacctc atatttgcag cgaaagatta
      901 ttcggcgtgc aaaagcaggg caaaaaaacc cttgttagaa agtttctttt ccaaaataaa
      961 aaattaaaaa ataaaagtat tttcaatatc aataacatac tctattctct aattatttat
     1021 tactaacttt tcaaagagat taacctaatc ttgtattcct taaattataa aatttacaaa
     1081 ttatatggat ccaaaaccaa atatcccatc gactcaagat ttaagttaaa agtttcttgg
     1141 acgaaacaca aagaaaagat attatcctaa cccaaaagat gacggcagcc ttagaaatca
     1201 ccgccactga ttaaatatcg gaatccaacg actctaattt cagacccttc atcaactttt
     1261 tattgacacg tgtcatattt tcgtccgttt gttatctaac aacccgtagc gttagccttc
     1321 cttttcgtga agaaaccttt tcttatccta taaatccacc cccaaatcca tcatcttcct
     1381 tcatcctgca atcatcttta tcttcaaagc tctgacttct tcaattctcc caaggtatta
     1441 tcttttttat tcttttatgt ttgtttagtt tttgaacagt ttattttcaa tcgattaaag
     1501 gttattattg tttttgatct gtttgtggct tttgatttaa gatataacct gatgttgtaa
     1561 ttaacacgat tgatagtgtc ttaatatgtt ttaattatgg tttctagatc taatcatcga
     1621 atttattaac gatctgttat agtttcgtgt tttcgatcgt ttttcaacaa gtcaattcca
     1681 aaatcgatgt tattgtataa aagcttcgaa cgacgatttt ttgtaaatta attcacgatc
     1741 taagttttca atttcagaga ataaagttgt tgatttgttt ttgaattgta atctgatctc
     1801 tgtttcaagt acgatatgaa tttgtttgtt gatgaattga tgataaaact gttttcgttt
     1861 tctaataatt gtttcgttct ttttttaaca gaatgtgaga ccacgaagtg gctcttcagt
     1921 ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1981 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2041 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2101 taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     2161 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg
     2221 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2281 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2341 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2401 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2461 gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2521 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2581 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2641 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2701 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2761 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2821 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2881 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2941 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     3001 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3061 cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg
     3121 tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3181 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3241 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3301 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3901 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3961 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4021 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4081 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtc
//
