LOCUS       sequence-492407-       10629 bp    DNA     circular SYN 10-NOV-2025
DEFINITION  Express Flag-HA-PUM2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Thomas A, Rehfeld F, Zhang H, Chang TC, Goodarzi M, Gillet F, 
            Mendell JT
  TITLE     RBM33 directs the nuclear export of transcripts containing GC-rich 
            elements.
  JOURNAL   Genes Dev. 2022 May 1;36(9-10):550-565. doi: 10.1101/gad.349456.122.
            Epub 2022 May 19.
  PUBMED    35589130
REFERENCE   2  (bases 1 to 10629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-05-1"; volume: "36(9-10):550-565. doi"; pages: " 
            10.1101/gad.349456.122. Epub 2022 May 19"
COMMENT     Sequence Label: pLJM1-FH-PUM2
FEATURES             Location/Qualifiers
     source          1..10629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    209..334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    827..1060
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1245..1289
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1587..1607
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1896..2099
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2050..2070
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2184..2207
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2208..2234
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5477..5594
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5643..6153
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6072..6090
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6175..6774
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6175..6194)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6671..6691
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6902..7135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7207..7328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7244..7263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7298..7317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7368..7503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7523..7542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7524..7542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7552..7569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7552..7568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7561..7583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7710..8165
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7797..7816)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8007..8028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8191..8295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8296..8364,8365..9156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8514..8533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9327..9915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9816..9835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10069..10086
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10203..10224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10239..10244,10245..10262,10263..10269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10277..10293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10282..10304
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10301..10317
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10301..10317
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10336..10356
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10338..10356
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10384..10610
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             join(10611..10629,1..162)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
       61 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      121 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      181 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      241 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      301 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      361 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      421 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      481 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
      541 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
      601 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
      661 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
      721 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
      781 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
      841 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
      901 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
      961 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1021 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1081 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1141 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1201 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1261 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1321 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1381 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1441 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1501 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     1561 agcctcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
     1621 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     1681 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     1741 aattatgttt taaaatggac tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1801 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1861 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     1921 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     1981 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     2041 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     2101 gtttagtgaa ccgtcagatc cgctagcgct accgggtaca agtccggact cagatctcga
     2161 gctcaagctt cgaattcgcc atggactaca aggacgacga tgacaagtac ccttatgacg
     2221 tgcccgatta cgctagcggc cgcaatcatg attttcaagc tcttgcatta gaatctcggg
     2281 gaatgggaga gcttttgcct accaaaaagt tttgggaacc tgatgattca acaaaagatg
     2341 gacaaaaagg catatttctt ggggatgatg aatggagaga gactgcatgg ggagcttctc
     2401 accattcaat gtcccagcct attatggtac agagaagatc tggacagggt tttcatggaa
     2461 acagtgaagt aaatgcaata ctgtctccgc gatcagaaag tggaggcctt ggtgtgagca
     2521 tggtagaata tgtattaagt tcttctcctg ctgataaatt ggattctcga tttaggaagg
     2581 gaaattttgg cactagagat gctgaaacag atggacctga gaaaggagat caaaaaggca
     2641 aggcttctcc atttgaggag gaccaaaaca gagatcttaa acaaggagat gatgatgatt
     2701 ctaaaataaa tggcagaggt ttgccaaatg gaatggatgc cgattgcaaa gattttaatc
     2761 gtactcctgg aagtcgtcaa gcctctccaa ctgaagtagt tgagcgcttg ggccccaata
     2821 ctaatccctc agaaggactg gggcctcttc ctaatcctac agctaataaa ccacttgttg
     2881 aagaattttc aaatcctgaa actcagaatc tggatgccat ggaacaagtt ggtctggaat
     2941 ccttacagtt tgactatcct ggtaatcagg taccaatgga ctcttcagga gctactgtag
     3001 gcctttttga ctacaattcc cagcagcagc tctttcagag gactaatgca ctaacagttc
     3061 aacagttaac tgcagctcaa cagcagcaat atgcattagc agcagctcag cagccacata
     3121 tagctggtgt attctcagca ggccttgctc cagctgcatt tgtgccaaat ccatacatta
     3181 ttagtgctgc tcctccaggg accgatccgt atactgcagc aggattggct gcagcagcta
     3241 cattagcagg tccagcagtg gttccacctc agtattacgg cgttccatgg ggggtgtatc
     3301 cagccaactt atttcagcag caagctgcag ctgcggcaaa taacacagcc agtcagcaag
     3361 cagcatcaca agctcagcct ggacagcaac aggttctccg tgctggagca ggtcagcgtc
     3421 ctcttactcc caatcagggt cagcaagggc agcaagcaga atcacttgcg gcagctgcag
     3481 cagcaaatcc aacattggct tttggtcagg gtcttgctac tggcatgcca ggctatcaag
     3541 tactagctcc aactgcctat tatgatcaga ctggtgcctt agtggttggc cctggagcaa
     3601 ggactggcct tggagctcca gttcggttaa tggctccaac acctgtttta attagttcag
     3661 cagcagcaca agctgcagca gcagcagcag ctggaggaac tgcaagtagc cttacaggca
     3721 gcacaaatgg tctgtttcgg ccaattggca ctcagccacc acagcagcag caacagcagc
     3781 caagcactaa tctgcaatct aattcatttt atggaagcag ttctttgact aatagctccc
     3841 agagtagttc tttattttct catggacctg gtcaacctgg aagtacatct cttggctttg
     3901 gaagtggtaa ctctttgggt gctgctatag gctcagccct cagtggattt ggttcatcag
     3961 ttggcagttc tgcaagtagt agtgccacaa ggagagagtc tctatctact agctctgact
     4021 tgtacaaaag atctagtagc agcctagcac ccatagggca accattttac aatagtctgg
     4081 gattttcctc ctctccaagt ccaataggca tgcctctgcc aagccaaact ccaggacatt
     4141 cacttacgcc accgccatca ctttcatcac atggatcctc atccagtttg catttaggag
     4201 gactgacaaa tggtagtggt cgatatatct ctgcagcacc tggagcagaa gcaaaatatc
     4261 gaagtgcttc aagcacttcc agtctattta gctccagcag ccagctcttt cctccttccc
     4321 ggcttcggta taataggtct gatattatgc cttctggccg cagtagatta ttggaagatt
     4381 tcagaaacaa ccgcttccca aaccttcagc ttagagactt gattggacat atagttgagt
     4441 tttctcaaga ccagcatggt tctagattca tacagcaaaa actagagaga gctactccag
     4501 ctgagcgaca gatggtattt aatgaaattc tgcaagcagc ctatcaatta atgactgatg
     4561 tttttggcaa ctatgttata cagaagtttt ttgagtttgg gagtctggat caaaaattag
     4621 ccctggctac tcgtattcgt ggtcatgttc tacccttagc cttgcagatg tatggctgcc
     4681 gcgttattca gaaagcatta gaatctattt cttctgacca gcagagtgaa atggtaaagg
     4741 agctggatgg tcatgtgctc aaatgtgtga aagatcagaa tggaaaccat gttgtacaaa
     4801 aatgtatcga atgtgttcag ccacagtcac tacagttcat cattgatgct ttcaagggac
     4861 aagtatttgt gctttcaact catccttatg gctgcagagt aattcagcgc atcctagagc
     4921 attgcactgc agaacagacc ttacctatct tagaagaact ccaccaacat acagagcagt
     4981 tggtacagga tcagtatggc aattatgtta ttcagcatgt actggaacac ggtcgacctg
     5041 aagacaagag caaaattgtt tccgaaatca ggggaaaggt tttagccctg agtcaacaca
     5101 aatttgccag caatgtagta gaaaagtgtg ttactcatgc ctcccgtgct gagagagctt
     5161 tactgattga cgaggtttgc tgccagaatg atggtcctca cagtgcctta tacaccatga
     5221 tgaaggacca gtatgccaat tacgtggttc aaaagatgat tgatatggct gaacctgctc
     5281 agagaaagat aatcatgcac aagattcgac ctcacattac tactttgcgc aaatacacat
     5341 acgggaagca tatactggcc aagttggaaa agtattattt gaagaatagc ccggacctag
     5401 gacctattgg aggaccacca aatggaatgc tgtaagaatt ctcgacctcg agacaaatgg
     5461 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5521 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5581 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     5641 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     5701 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     5761 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     5821 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     5881 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     5941 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     6001 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     6061 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     6121 ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc
     6181 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6241 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6301 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6361 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6421 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6481 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6541 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6601 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6661 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6721 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     6781 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta
     6841 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6901 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6961 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7021 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7081 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7141 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7201 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7261 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7321 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     7381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7441 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7501 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     7561 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7621 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7681 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     7741 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7801 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7861 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7921 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7981 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8041 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8101 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8161 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8221 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8281 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8341 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8401 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8461 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8521 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8581 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8641 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8701 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8761 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8821 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8881 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8941 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9001 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9061 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9121 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9181 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9241 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9301 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9361 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9421 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9481 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9541 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9601 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9661 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9721 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9781 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9841 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9901 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9961 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10021 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10081 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10141 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10201 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10261 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    10321 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
    10381 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
    10441 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
    10501 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
    10561 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
    10621 ggttagacc
//
