LOCUS       sequence-492409-       12093 bp    DNA     circular SYN 10-NOV-2025
DEFINITION  Lux constitutive Golden Gate construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 4119 to 4153)
  TITLE     Murray Lab Golden Gate Plasmids 2025
REFERENCE   2  (bases 1 to 12093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: eOYW023
FEATURES             Location/Qualifiers
     source          1..12093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1210..1375
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(1494..1513)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1505..2320
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1505..2320
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1578..1597)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4034..4052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4119..4153
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             4162..4173
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4180..5622
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             5635..6558
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDIT*PS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             6607..7689
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             7704..8687
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             8866..9978
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      9983..10077
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      10482..10870
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(11041..11412)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11445..11554
                     /note="incP origin of transfer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgaattaatt cgagctcgcc caaacataac aggaagaaaa
     1201 atgccccgct gtgggcggac aaaatagttg ggaactggga ggggtggaaa tggagttttt
     1261 aaggattatt tagggaagag tgacaaaata gatgggaact gggtgtagcg tcgtaagcta
     1321 atacgaaaat taaaaatgac aaaatagttt ggaactagat ttcacttatc tggttggcct
     1381 gcaggggggg ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1441 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     1501 tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat
     1561 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1621 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1681 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     1741 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1801 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1861 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1921 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1981 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     2041 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
     2101 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     2161 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     2221 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     2281 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     2341 gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga
     2401 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     2461 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     2521 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     2581 ccttcttcac gaggcagacc tcagcgcccc cccccccctg caggtcgact agagtcgggg
     2641 atccggtgat tgattgagca agctttatgc ttgtaaaccg ttttgtgaaa aaatttttaa
     2701 aataaaaaag gggacctcta gggtccccaa ttaattagta atataatcta ttaaaggtca
     2761 ttcaaaaggt catccaccgg atcaattccc ctgctcgcgc aggctgggtg ccaagctctc
     2821 gggtaacatc aaggcccgat ccttggagcc cttgccctcc cgcacgatga tcgtgccgtg
     2881 atcgaaatcc agatccttga cccgcagttg caaaccctca ctgatccgca tgcccgttcc
     2941 atacagaagc tgggcgaaca aacgatgctc gccttccaga aaaccgagga tgcgaaccac
     3001 ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg
     3061 aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg ccgccaatgc
     3121 ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc
     3181 gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg
     3241 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     3301 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     3361 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     3421 agcgcgttac gccgtgggtc gatgtttgtg tctaacaatt cgttcaagcc gacgccgctt
     3481 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     3541 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     3601 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
     3661 caacatccgc attaaaatct agcgagggct ttactaagct gatccggtgg atgacctttt
     3721 gaatgacctt taatagatta tattactaat taattgggga ccctagaggt cccctttttt
     3781 attttaaaaa ttttttcaca aaacggttta caagcataaa gcttgctcaa tcaatcaccg
     3841 gatcccgacg ggcccggtac cgcggccgcg cgaattcgag ctcggtacca tttatcaggg
     3901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4021 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agaacattac
     4081 tcgcatccat tctcaggctg tctcgtctcg tctcggagtt gacggctagc tcagtcctag
     4141 gtacagtgct agctactaga gaaagaggag aaatactaaa tgactaaaaa aatttcattc
     4201 attattaacg gccaggttga aatctttccc gaaggtgatg atttagtgca atccattaat
     4261 tttggtgata atagtgttta cctgccaata ttgaatgact ctcatgtaaa aaacattatt
     4321 gattgtaatg gaaataacga attacggttg cataacattg tcaattttct ctatacggta
     4381 gggcaaagat ggaaaaatga agaatactca agacgcagga catacattcg tgacttaaaa
     4441 aaatatatgg gatattcaga agaaatggct aagctagagg ccaattggat atctatgatt
     4501 ttatgttcta aaggcggcct ttatgatgtt gtagaaaatg aacttggttc tcgccatatc
     4561 atggatgaat ggctacctca ggatgaaagt tatgttcggg cttttccgaa aggtaaatct
     4621 gtacatctgt tggcaggtaa tgttccatta tctgggatca tgtctatatt acgcgcaatt
     4681 ttaactaaga atcagtgtat tataaaaaca tcgtcaaccg atccttttac cgctaatgca
     4741 ttagcgttaa gttttattga tgtagaccct aatcatccga taacgcgctc tttatctgtt
     4801 atatattggc cccaccaagg tgatacatca ctcgcaaaag aaattatgca acatgcggat
     4861 gttattgtcg cttggggagg gccagatgcg attaattggg cggtagagca tgcgccatct
     4921 tatgctgatg tgattaaatt tggttctaaa aagagtcttt gcattatcga taatcctgtt
     4981 gatttgacgt ccgcagcgac aggtgcggct catgatgttt gtttttacga tcagcgagct
     5041 tgtttttctg cccaaaacat atattacatg ggaaatcatt atgaggaatt taagttagcg
     5101 ttgatagaaa aacttaatct atatgcgcat atattaccga atgccaaaaa agattttgat
     5161 gaaaaggcgg cctattcttt agttcaaaaa gaaagcttgt ttgctggatt aaaagtagag
     5221 gtggatattc atcaacgttg gatgattatt gagtcaaatg caggtgtgga atttaatcaa
     5281 ccacttggca gatgtgtgta ccttcatcac gtcgataata ttgagcaaat attgccttat
     5341 gttcaaaaaa ataagacgca aaccatatct atttttcctt gggagtcatc atttaaatat
     5401 cgagatgcgt tagcattaaa aggtgcggaa aggattgtag aagcaggaat gaataacata
     5461 tttcgagttg gtggatctca tgacgggatg aggccgttgc aacgattagt gacatatatt
     5521 tctcatgaaa ggccatctaa ctatacggct aaggatgttg cggttgaaat agaacagact
     5581 cgattcctgg aagaagataa gttccttgta tttgtcccat aataggtaaa aagtatggaa
     5641 aatgaatcaa aatataaaac catcgaccac gttatttgtg ttgaaggaaa taaaaaaatt
     5701 catgtttggg aaacgctgcc agaagaaaac agcccaaaga gaaagaatgc cattattatt
     5761 gcgtctggtt ttgcccgcag gatggatcat tttgctggtc tggcggaata tttatcgcgg
     5821 aatggatttc atgtgatccg ctatgattcg cttcaccacg ttggattgag ttcagggaca
     5881 attgatgaat ttacaatgtc tataggaaag cagagcttgt tagcagtggt tgattggtta
     5941 actacacgaa aaataaataa cttcggtatg ttggcttcaa gcttatctgc gcggatagct
     6001 tatgcaagcc tatctgaaat caatgcttcg tttttaatca ccgcagtcgg tgttgttaac
     6061 ttaagatatt ctcttgaaag agctttaggg tttgattatc tcagtctacc cattaatgaa
     6121 ttgccgaata atctagattt tgaaggccat aaattgggtg ctgaagtctt tgcgagagat
     6181 tgtcttgatt ttggttggga agatttagct tctacaatta ataacatgat gtatcttgat
     6241 ataccgttta ttgcttttac tgcaaataac gataattggg tcaagcaaga tgaagttatc
     6301 acattgttat caaatattcg tagtaatcga tgcaagatat attctttgtt aggaagttcg
     6361 catgacttga gtgaaaattt agtggtcctg cgcaattttt atcaatcggt tacgaaagcc
     6421 gctatcgcga tggataatga tcatctggat attgatgttg atattactta accgtcattt
     6481 gaacatttaa ctattgcgac agtcaatgaa cgccgaatga gaattgagat tgaaaatcaa
     6541 gcaatttctc tgtcttaaaa tctattgaga tattctatca ctcaaatagc aatataagga
     6601 ctctctatga aatttggaaa ctttttgctt acataccaac ctccccaatt ttctcaaaca
     6661 gaggtaatga aacgtttggt taaattaggt cgcatctctg aggagtgtgg ttttgatacc
     6721 gtatggttac tggagcatca tttcacggag tttggtttgc ttggtaaccc ttatgtcgct
     6781 gctgcatatt tacttggcgc gactaaaaaa ttgaatgtag gaactgccgc tattgttctt
     6841 cccacagccc atccagtacg ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa
     6901 ggacgatttc ggtttggtat ttgccgaggg ctttacaaca aggactttcg cgtattcggc
     6961 acagatatga ataacagtcg cgccttagcg gaatgctggt acgggctgat aaagaatggc
     7021 atgacagagg gatatatgga agctgataat gaacatatca agttccataa ggtaaaagta
     7081 aaccccgcgg cgtatagcag aggtggcgca ccggtttatg tggtggctga atcagcttcg
     7141 acgactgagt gggctgctca atttggccta ccgatgatat taagttggat tataaatact
     7201 aacgaaaaga aagcacaact tgagctttat aatgaagtgg ctcaagaata tgggcacgat
     7261 attcataata tcgaccattg cttatcatat ataacatctg tagatcatga ctcaattaaa
     7321 gcgaaagaga tttgccggaa atttctgggg cattggtatg attcttatgt gaatgctacg
     7381 actatttttg atgattcaga ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt
     7441 gactttgtat taaaaggaca taaagatact aatcgccgta ttgattacag ttacgaaatc
     7501 aatcccgtgg gaacgccgca ggaatgtatt gacataattc aaaaagacat tgatgctaca
     7561 ggaatatcaa atatttgttg tggatttgaa gctaatggaa cagtagacga aattattgct
     7621 tccatgaagc tcttccagtc tgatgtcatg ccatttctta aagaaaaaca acgttcgcta
     7681 ttatattagc taaggagaaa gaaatgaaat ttggattgtt cttccttaac ttcatcaatt
     7741 caacaactgt tcaagaacaa agtatagttc gcatgcagga aataacggag tatgttgata
     7801 agttgaattt tgaacagatt ttagtgtatg aaaatcattt ttcagataat ggtgttgtcg
     7861 gcgctcctct gactgtttct ggttttctgc tcggtttaac agagaaaatt aaaattggtt
     7921 cattaaatca catcattaca actcatcatc ctgtccgcat agcggaggaa gcttgcttat
     7981 tggatcagtt aagtgaaggg agatttattt tagggtttag tgattgcgaa aaaaaagatg
     8041 aaatgcattt ttttaatcgc ccggttgaat atcaacagca actatttgaa gagtgttatg
     8101 aaatcattaa cgatgcttta acaacaggct attgtaatcc agataacgat ttttatagct
     8161 tccctaaaat atctgtaaat ccccatgctt atacgccagg cggacctcgg aaatatgtaa
     8221 cagcaaccag tcatcatatt gttgagtggg cggccaaaaa aggtattcct ctcatcttta
     8281 agtgggatga ttctaatgat gttagatatg aatatgctga aagatataaa gccgttgcgg
     8341 ataaatatga cgttgaccta tcagagatag accatcagtt aatgatatta gttaactata
     8401 acgaagatag taataaagct aaacaagaga cgcgtgcatt tattagtgat tatgttcttg
     8461 aaatgcaccc taatgaaaat ttcgaaaata aacttgaaga aataattgca gaaaacgctg
     8521 tcggaaatta tacggagtgt ataactgcgg ctaagttggc aattgaaaag tgtggtgcga
     8581 aaagtgtatt gctgtccttt gaaccaatga atgatttgat gagccaaaaa aatgtaatca
     8641 atattgttga tgataatatt aagaagtacc acatggaata tacctaatag atttcgagtt
     8701 gcagcgaggc ggcaagtgaa cgaatcccca ggagcataga taactatgtg actggggtga
     8761 gtgaaagcag ccaacaaagc agcagcttga aagatgaagg gtataaaaga gtatgacagc
     8821 agtgctgcca tactttctaa tattatcttg aggagtaaaa caggtatgac ttcatatgtt
     8881 gataaacaag aaattacagc aagctcagaa attgatgatt tgattttttc gagcgatcca
     8941 ttagtgtggt cttacgacga gcaggaaaaa atcagaaaga aacttgtgct tgatgcattt
     9001 cgtaatcatt ataaacattg tcgagaatat cgtcactact gtcaggcaca caaagtagat
     9061 gacaatatta cggaaattga tgacatacct gtattcccaa catcggtttt taagtttact
     9121 cgcttattaa cttctcagga aaacgagatt gaaagttggt ttaccagtag cggcacgaat
     9181 ggtttaaaaa gtcaggtggc gcgtgacaga ttaagtattg agagactctt aggctctgtg
     9241 agttatggca tgaaatatgt tggtagttgg tttgatcatc aaatagaatt agtcaatttg
     9301 ggaccagata gatttaatgc tcataatatt tggtttaaat atgttatgag tttggtggaa
     9361 ttgttatatc ctacgacatt taccgtaaca gaagaacgaa tagattttgt taaaacattg
     9421 aatagtcttg aacgaataaa aaatcaaggg aaagatcttt gtcttattgg ttcgccatac
     9481 tttatttatt tactctgcca ttatatgaaa gataaaaaaa tctcattttc tggagataaa
     9541 agcctttata tcataaccgg aggcggctgg aaaagttacg aaaaagaatc tctgaaacgt
     9601 gatgatttca atcatctttt atttgatact ttcaatctca gtgatattag tcagatccga
     9661 gatatattta atcaagttga actcaacact tgtttctttg aggatgaaat gcagcgtaaa
     9721 catgttccgc cgtgggtata tgcgcgagcg cttgatcctg aaacgttgaa acctgtacct
     9781 gatggaacgc cggggttgat gagttatatg gatgcgtcag caaccagtta tccagcattt
     9841 attgttaccg atgatgtcgg gataattagc agagaatatg gtaagtatcc cggcgtgctc
     9901 gttgaaattt tacgtcgcgt caatacgagg acgcagaaag ggtgtgcttt aagcttaacc
     9961 gaagcgtttg atagttgaag gtgactcctg ttgatagatc cagtaatgac ctcagaactc
    10021 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatgct
    10081 tccaggatac atagattacc acaactccga gcccttccac cccaagctag cttggaggac
    10141 aaatccgccg ctaggagctt gcggccgcct taagacgcgt ggatccccgg gaatttcgac
    10201 cccacgcccc tctttaatac gacgggcaat ttgcacttca gaaaatgaag agtttgcttt
    10261 agccataaca aaagtccagt atgctttttc acagcataac tggactgatt tcagtttaca
    10321 actattctgt ctagtttaag actttattgt catagtttag atctattttg ttcagtttaa
    10381 gactttattg tccgcccaca cccgcttatg cagggcatcc atttattact caaccgtaac
    10441 cgattttgcc aggttacgcg gctggtcctc tagaattccc atgtcagccg ttaagtgttc
    10501 ctgtgtcact caaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg
    10561 gcttgttgtc cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt
    10621 tcttataaaa cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg
    10681 ttcaaacatg agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct
    10741 tagtacgtta gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg
    10801 agagcttagt acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctga
    10861 tcttcagatc ctctacgccg gacgcatcgt ggccggatcg gggggggggg gcgctcggtc
    10921 ttgccttgct cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag
    10981 cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag
    11041 tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt
    11101 ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg
    11161 gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg
    11221 ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac
    11281 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct
    11341 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt
    11401 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
    11461 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
    11521 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
    11581 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
    11641 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc gctttctcat
    11701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12061 cagcagatta cgcgcagaaa aaaaggatct caa
//
