LOCUS       sequence-492445-        8107 bp    DNA     circular SYN 10-NOV-2025
DEFINITION  Expresses histone H2B fused with mRFP1, together with monomeric 
            EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Hihara S, Pack CG, Kaizu K, Tani T, Hanafusa T, Nozaki T, Takemoto 
            S, Yoshimi T, Yokota H, Imamoto N, Sako Y, Kinjo M, Takahashi K, 
            Nagai T, Maeshima K
  TITLE     Local nucleosome dynamics facilitate chromatin accessibility in 
            living mammalian cells.
  JOURNAL   Cell Rep. 2012 Dec 27;2(6):1645-56. doi: 
            10.1016/j.celrep.2012.11.008. Epub 2012 Dec 13.
  PUBMED    23246002
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2012.11.008"; journalName: "Cell Rep"; date: 
            "2012-12-27- 27"; volume: "2"; issue: "6"; pages: "1645-56"
COMMENT     Sequence Label: pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT
FEATURES             Location/Qualifiers
     source          1..8107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..60
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(227..1249)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(227..1249)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(1257..1304)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1436..1455
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1588..1812
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1801..1818
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(1943..2653,2654..2656,2657..2659))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1943..2653,2654..2656,2657..2659))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1985..2006)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2334..2353
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2593..2614
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2656..2665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3593..4270)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(3593..4270)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3655..3678)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(3853..3872)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4122..4140
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(4271..4291)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        complement(4676..5854)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4685..5623
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4708..4728)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5908..6012
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6013..6081,6082..6873)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6231..6250)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7044..7632
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7533..7552
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7786..7803
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7920..7941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7956..7961,7962..7979,7980..7986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7994..8010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7999..8021
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8018..8034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8018..8034
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    join(8083..8107,1..97)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(8094..8107,1..6))
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
       61 tttatttgta accattataa gctgcaataa acaagttggg gtgggcgaag aactccagca
      121 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
      181 acctttcata gaaggcggcg gtggaatcga aatctcgtag tacgtgctat tcctttgccc
      241 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
      301 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
      361 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
      421 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
      481 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
      541 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
      601 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
      661 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
      721 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
      781 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
      841 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
      901 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
      961 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     1021 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     1081 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     1141 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     1201 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttg gccaaggaag
     1261 ttcctatact ttctagagaa taggaacttc ggaataggaa cttctaggta cgtgaaccat
     1321 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1381 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1441 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1501 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     1561 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     1621 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     1681 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     1741 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     1801 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac tcgagcggcc
     1861 gccactgtgc tggatccggt agcgggcccg cggtaccgtc gactgcagaa ttcgaagctt
     1921 gagctcgaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg
     1981 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     2041 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     2101 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     2161 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     2221 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     2281 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     2341 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     2401 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     2461 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     2521 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     2581 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     2641 cctcgccctt gctcaccatg gtggcgaccg gatctgcaga attccaccac actggactag
     2701 tggatccgag ctcggtacca agcttcggct tattattccg cggcggccgc agcggcagct
     2761 acaacaaccg cgtcgctctc cgctcaattt ccaagagcca gctttgaagc caagtgcgag
     2821 cagtttcaaa ctcaccgcgc taaagggccc cggattcacc aatcgggtag cccgtagact
     2881 ttcaaagcag ccaatcagag cccagctacg ctgggcaggc cttcccgggt ggctagagcg
     2941 cgaaagaaag aggaaagggc ggctagagaa aaagcaggag ggcgggcgcc aactgagtgc
     3001 gagcgcaagc gctctcctcc agtcgggaga gtgtcgtcct actgtttcta gtcagcggag
     3061 caggaagcta ctgttcgctc cgttcttctt ttaaattttt tctcccagca ttggcacagt
     3121 tcaaatttat tatactcaaa atagctcatc aaaaaagtga tattgtgttt acatcgagat
     3181 tccattactt tcactcctaa tacttagggt taggagtgta tagttatgtt tttctaaatg
     3241 cgtgatttgc gggctggctc caaggagcac atttcagtga ccttaagaag gaaatggaaa
     3301 actcaaaaga ccgcctcaaa aatgtaaagg aaaatttatt atttatatcg ctgtgctttg
     3361 tttctacctc atttttgaat ttaatattaa attattttat tatttacatt ttgtttatta
     3421 tacaattaaa aacatttgaa atgtattaaa ttttaaaata ttttcacatc agaattttaa
     3481 atatatagag agaggcgtgc atttagagac tgggtctcat tatgttgcgc atgctggcct
     3541 ccaaatcctg ggctcaagca atcggcctca gcctccagcg cagctgaagc ttttaggcgc
     3601 cggtggagtg gcggccctcg gcgcgctcgt actgttccac gatggtgtag tcctcgttgt
     3661 gggaggtgat gtccagcttg atgtcggtct tgtaggcgcc gggcagctgc acgggcttct
     3721 tggccatgta ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc agcttcagcc
     3781 tcatcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gtggaggcct
     3841 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
     3901 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
     3961 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
     4021 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tactggaact
     4081 gaggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagcttgg
     4141 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
     4201 cgttcacgga gccctccatg cgcaccttga agcgcatgaa ctccttgatg acgtcctcgg
     4261 aggaggccat caactttatt atacaaagtt gcttagcgct ggtgtacttg gtgacggcct
     4321 tggtgccctc ggacacggcg tgcttggcca actccccggg cagcagcagg cgcacggccg
     4381 tctggatctc cctggaggtg atggtcgagc gcttgttgta atgcgccagg cgggaagcct
     4441 cacccgcgat gcgttcgaag atgtcgttga cgaaggagtt catgattccc atggccttag
     4501 aggagatgcc ggtgtcgggg tggacctgct tcagcacctt gtacacgtat acggagtagc
     4561 tctccttgcg gctgcgcttg cgcttcttgc cgtccttctt ctgcgcctta gtcacggctt
     4621 tcttcgagcc cttcttgggc gcgggagcag acttcgctgg ctctggcatg ctagctcacg
     4681 acacctgaaa tggaagaaaa aaactttgaa ccactgtctg aggcttgaga atgaaccaag
     4741 atccaaactc aaaaagggca aattccaagg agaattacat caagtgccaa gctggcctaa
     4801 cttcagtctc cacccactca gtgtggggaa actccatcgc ataaaacccc tccccccaac
     4861 ctaaagacga cgtactccaa aagctcgaga actaatcgag gtgcctggac ggcgcccggt
     4921 actccgtgga gtcacatgaa gcgacggctg aggacggaaa ggcccttttc ctttgtgtgg
     4981 gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca ttttgagctc cctgcagcag
     5041 ggccgggaag cggccatctt tccgctcacg caactggtgc cgaccgggcc agccttgccg
     5101 cccagggcgg ggcgatacac ggcggcgcga ggccaggcac cagagcaggc cggccagctt
     5161 gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag gccccgcctc gccgaacatg
     5221 tgcgctggga cgcacgggcc ccgtcgccgc ccgcggcccc aaaaaccgaa ataccagtgt
     5281 gcagatcttg gcccgcattt acaagactat cttgccagaa aaaaagcgtc gcagcaggtc
     5341 atcaaaaatt ttaaatggct agagacttat cgaaagcagc gagacaggcg cgaaggtgcc
     5401 accagattcg cacgcggcgg ccccagcgcc caggccaggc ctcaactcaa gcacgaggcg
     5461 aaggggctcc ttaagcgcaa ggcctcgaac tctcccaccc acttccaacc cgaagctcgg
     5521 gatcaagaat cacgtactgc agccaggtgg aagtaattca aggcacgcaa gggccataac
     5581 ccgtaaagag gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa
     5641 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     5701 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     5761 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg
     5821 atgtgcgctc tgcccactga cgggcaccgg agcctcacgg gatctcccga tccgtcgacg
     5881 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5941 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6001 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6061 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6121 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6181 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6241 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6301 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6361 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6421 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6481 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6541 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6601 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6661 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6721 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6781 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6841 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6901 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6961 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7021 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7081 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7141 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7201 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7261 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7381 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7441 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7561 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7621 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7681 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7741 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7801 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7921 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7981 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8041 tacgccaagc tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt
     8101 ttggaca
//
