LOCUS       sequence-492622-        6842 bp    DNA     circular SYN 11-NOV-2025
DEFINITION  timestamp monomer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6842)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 6842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: pAAV-UBC-1POK-dMBP-HA-Halotag
FEATURES             Location/Qualifiers
     source          1..6842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     promoter        274..672
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             2943..2969
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2979..3869
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     polyA_signal    3894..4101
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4271..4400
                     /label=AAV2 ITR
     rep_origin      complement(4637..5225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4717..4736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5396..6187,6188..6256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6019..6038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6257..6361)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6387..6842)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6524..6545)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6736..6755
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaggcctcc gcgccgggtt ttggcgcctc
      301 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      361 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      421 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      481 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      541 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      601 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      661 atcgtcactt ggcgactagt gccaccatga ttgactatac tgctgctggt ttcaccctcc
      721 tccaaggtgc acatctgtac gctcctgaag acagaggtat ctgcgatgtg ctggttgcca
      781 acggtaagat catcgctgtg gcctccaaca ttcctagcga catcgttcca aactgcaccg
      841 ttgtcgatct gagcggacag atcctctgtc caggattcat tgatcagcac gtgcacctca
      901 ttggaggagg aggcgaagct ggtcctacta ctagaactcc cgaagtcgca ctgtctcgcc
      961 tcacagaggc aggcgttacc tccgtcgtgg gactcctggg tactgatagc atctctaggc
     1021 accctgagtc tctgctggca aagaccagag ccctcaatga ggagggtatc tccgcttgga
     1081 tgctgactgg tgcttatcac gtcccatctc gcaccattac tggcagcgtg gagaaggacg
     1141 ttgcaatcat cgacagagtg atcggtgtta agtgcgctat ctccgaccac aggagcgctg
     1201 ctcccgacgt ctatcacctg gccaacatgg cagccgagag cagagtcggt ggtctgctcg
     1261 gtggcaagcc aggagttacc gtgttccaca tgggtgactc taagaaggca ctgcaaccca
     1321 tctatgacct gctggagaac tgtgacgtcc caatctccaa gctgctgcct acccacgtca
     1381 acaggaacgt gcctctcttc taccaagctc tggagttcgc aagaaagggt ggcaccatcg
     1441 acatcacctc tagcattgat gaacccgtcg ctccagctga gggaatcgca agggctgtcc
     1501 aagcaggtat cccactggca agggtgacac tcagctctga tggcaacggc tcccagccat
     1561 tcttcgatga tgaaggaaat ctgactcaca ttggcgtggc tggatttgaa actctcctgg
     1621 aaacagttca ggttctggtt aaggactacg acttcagcat ttctgacgct ctcagaccac
     1681 tcacctctag cgtcgctggc ttcctgaacc tgactggtaa aggcgaaatc aaacctggta
     1741 atgacgctga tctgctggtg atgacacccg aactgcgcat tgagcaggtc tatgcaagag
     1801 gaaagctgat ggttaaggac ggaaaggctt gtgtcaaggg cacattcgag actgctggca
     1861 aactggttat ctggatcaat ggcgataagg gctacaatgg cctggcagag gtcggtaaga
     1921 agttcgagaa agatacaggc atcaaagtta ctgtggaaca cccagataag ctggaggaga
     1981 agttccctca ggtcgcagca actggcgacg gtccagacat catcttctgg gcacacgata
     2041 ggttcggtgg ctatgcccaa tctggcctgc tggctgagat tactcctgat aaggctttcc
     2101 aggacaagct gtatccattc acctgggatg ctgtgagata caatggcaag ctgatcgcat
     2161 accctattgc tgtggaagct ctgagcctga tctacaacaa ggacctgctg cctaatcctc
     2221 ctaagacatg ggaagaaatc cctgcactgg acaaggaact gaaggccaaa ggcaagtccg
     2281 cactgatgtt caacctgcag gagccttact ttacctggcc actgattgct gccgacggtg
     2341 gttacgcttt caagtacgag aatggtaagt acgacatcaa agatgtgggt gtggacaatg
     2401 ctggtgccaa agctggtctg actttcctgg tggatctgat caagaacaag cacatgaatg
     2461 cagacactga ctattctatc gcagaggctg ctttcaacaa aggcgaaacc gcaatgacta
     2521 tcaatggtcc ttgggcatgg tctaacatcg acactagcaa agtcaactac ggtgtcaccg
     2581 ttctgccaac cttcaagggt cagccaagca aacctttcgt tggcgtgctg agcgcaggta
     2641 tcaacgctgc ctctcctaac aaagagctgg ctaaggagtt tctggagaac tacctgctga
     2701 cagacgaagg tctggaggca gtgaacaagg acaagccact gggtgccgtg gctctgaaga
     2761 gctacgaaga agaactggcc aaggaccctc gcatcgctgc tacaatggag aacgcacaga
     2821 agggtgagat catgccaaac atcccacaga tgagcgcatt ctggtatgcc gtgaggaccg
     2881 ctgttatcaa cgcagcttct ggcagacaga ccgtggatga agccctgaaa gacgcacaga
     2941 cctacccata tgacgtccct gactatgcag gctctggtat ggcagaaatc ggtactggct
     3001 ttccattcga cccccactat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
     3061 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
     3121 ggcgcaacat catcccgcac gttgcaccga cccaccgctg cattgctcca gacctgatcg
     3181 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
     3241 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
     3301 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
     3361 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
     3421 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
     3481 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     3541 actaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     3601 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     3661 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     3721 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     3781 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     3841 gctggctgtc gacgctcgag atttccggct aagcggccgc tcataaagct cgactgtgcc
     3901 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3961 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4021 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4081 caatagcagg catgctgggg atgcggtccg gactgtacaa ttgcattcat tttatgtttc
     4141 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4201 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     4261 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4321 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4381 gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4441 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4501 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4561 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4621 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4681 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4741 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4801 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4861 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4921 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4981 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5041 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5101 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5161 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5221 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5281 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5341 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5401 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5461 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5521 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5581 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5641 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5701 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5761 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5821 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5881 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5941 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6001 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6061 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6361 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     6421 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6481 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6541 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6601 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     6661 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6721 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     6781 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     6841 gt
//
