LOCUS       sequence-492630-       11119 bp    DNA     circular SYN 12-NOV-2025
DEFINITION  A phase separating fluorescent protein with optogenetic and 
            chemogenetic tags for recruitment of desired cargo domains. Used to 
            demonstrate condensate dissolution using the OptoMBP system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Brumbaugh-Reed EH, Aoki K, Toettcher JE
  TITLE     Rapid and reversible dissolution of biomolecular condensates using 
            light-controlled recruitment of a solubility tag
  JOURNAL   bioRxiv 2024.01.16.575860
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.16.575860"
COMMENT     Sequence Label: pHR myc-FUS-FUS-FusionRed-FUS-FRB-iLID
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(505..834)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      519..654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     819..839
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(909..926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1080..1668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1160..1179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1839..2630,2631..2699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2462..2481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2700..2804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2872..2890
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2928..2950)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3050..3069
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3283..3302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3592..3610
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3592..3609
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             3635..3868
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3958..3974
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3959..3975
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3978..4566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4084..4095
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4493..4513
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4670..5107)
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             complement(5150..5428)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             complement(5685..5696)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(6336..6347)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(6740..6769)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(6821..7228)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    7382..7499
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7784..7828)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8013..8246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8743..8868
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8915..9548
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9716..9850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9740..9759)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9794..9813
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10275..10295)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          10425..10490
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc
       61 ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta
      121 gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc
      181 cggtacagac cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt
      241 cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg
      301 ctcatgtgaa gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata
      361 ggttgcgcga gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg
      421 tctttttacg cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg
      481 caagcctgtc ttgtgtccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca
      541 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
      601 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
      661 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
      721 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
      781 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
      841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2821 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     2881 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     3061 cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct gcattaggaa
     3121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     3181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     3241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     3301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     3361 gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccgggt gcgggacggc
     3421 gttaccagaa actcagaagg ttcgtccaac caaaccgact ctgacggcag tttacgagag
     3481 agatgatagg gtctgcttca gtaagccaga tgctacacaa ttaggcttgt acatattgtc
     3541 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     3601 acactataga atacacggaa ttaattctag atgctgctag agattttcca cactgactaa
     3661 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     3721 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     3781 tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt
     3841 cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga
     3901 tctacagctg ccttgtaagt cattggtctt aaaggtaccg agctcgaatt aattccctcg
     3961 aggtcgacgg tatcgatgcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
     4021 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
     4081 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
     4141 acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt
     4201 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
     4261 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc
     4321 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac
     4381 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa
     4441 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt
     4501 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
     4561 ttgattatcg ataagcttga tatcaagctt gcatgcctgc aggtcgactc tagagtcgcg
     4621 gcctcagtca gtcacgcatg ttgcaggtgg gagttgcggc cgcttattaa aagtaatttt
     4681 cgtcgttcgc tgcctcagca atctgaaagg ctgtcttttt gatcaggcag accgcctcac
     4741 gctcagcggc gccatgaaga cgttcggtgc catccagctg cacgccaatg aagtactgga
     4801 cgtcgccctt ataatcgcgc atcggctgca ggtggaacac gttccagaac ttcttgccgc
     4861 tcttggtata attgatcagc tgaacggtca cttcagtctg gttgtcaata gcatctctga
     4921 ttttccgaac agtggcacgg tctgtctcgg gtccctgcag aaaacggcaa ttgcgcccca
     4981 ggatctcttc ccgagaatat tctgtcagct gcaggaagga atcgctcgca aaaatgattg
     5041 ggttgtcagg cagtctcgga tcagtaatca cgaaattttt ctcgatccgt tccagtgtgg
     5101 ttgccagaaa ctcaccggat ccaccaccac cagcactacc tccgcttccc tttgagattc
     5161 gtcggaacac atgataatag aggtcccagg cttggaggag gtccttgaca ttccctgatt
     5221 tcatgtactt cctgcaccac tcttgggcct ccattaaatc tcgaccatag gcctgattaa
     5281 aggatgtttc cttcagagtc tgggggcccc gttccatcat agcatgcaag ggctccagca
     5341 cctcaaacat gcctttcacg ttcctttccc caaagtacaa acgagatgcc tcttccaggc
     5401 cttcatgcca catctcatgc cagaggatcc cgcttccacg gtcctgctgt ccatagccac
     5461 cgctgccacc tccaccactc tggtcttgat tgccataacc gccaccactg ccaccaccac
     5521 tactcatgga ggattgatct tggccatagt tacctccacc tccacctcca cctccaccac
     5581 cactgctgct gttgtactgg ttctgctgtc catagccctg agggggatta tagctttgct
     5641 gctgtccata gctttgctgc tgtccaccat agctaggctg ctggctgtag ctcccactct
     5701 ggggctgccc atagctgctg ctctgagaac tgctaccgta acttcccgag gtgctgctgg
     5761 gagctggctg ctggccatag ccaggatagg aggactgctg cccgtaagac gattgggagc
     5821 tctggctact gccatagccg ccagtcgagc catatccctg gggagttgac tgagttccat
     5881 agcctgtgtt ctggctctgg ccataagaag aatagctgct ctggccatag cctgaagtgt
     5941 ccgtggactg gctataacca ctgtaactct gctgtccgta gggctgactg ctctgctggg
     6001 aatagccctg cccgggctgg gtggggtagg ccccatagct ttgggttgct tgttgggtat
     6061 aatcgtttga ggccattcct ccacctccac ggtcctgctg tccatagcca ccgctgccac
     6121 ctccaccact ctggtcttga ttgccataac cgccaccact gccaccacca ctactcatgg
     6181 aggattgatc ttggccatag ttacctccac ctccacctcc acctccacca ccactgctgc
     6241 tgttgtactg gttctgctgt ccatagccct gagggggatt atagctttgc tgctgtccat
     6301 agctttgctg ctgtccacca tagctaggct gctggctgta gctcccactc tggggctgcc
     6361 catagctgct gctctgagaa ctgctaccgt aacttcccga ggtgctgctg ggagctggct
     6421 gctggccata gccaggatag gaggactgct gcccgtaaga cgattgggag ctctggctac
     6481 tgccatagcc gccagtcgag ccatatccct ggggagttga ctgagttcca tagcctgtgt
     6541 tctggctctg gccataagaa gaatagctgc tctggccata gcctgaagtg tccgtggact
     6601 ggctataacc actgtaactc tgctgtccgt agggctgact gctctgctgg gaatagccct
     6661 gcccgggctg ggtggggtag gccccatagc tttgggttgc ttgttgggta taatcgtttg
     6721 aggccataga ccctcctccc agatcctctt ctgagatgag tttttgttcc atggtagctc
     6781 cgatcacacg cgtgagaatt ctcgagagct ccagatcccc cccgggcgac tcagtctgtc
     6841 ggaggactgg cgcgccgagt gaggggttgt gagctctttt atagagctcg ggaagcagaa
     6901 gcgcgcgaac agaagcgaga agcaggctga ttggttaatt caaataaggc gcagggtcat
     6961 ttcaggtcct tgggggagcc tggaaacatc tgatgggtct taagaaactg ctgagggttg
     7021 ggccatatct ggggaccatc tgttcttggc ctcgggccgg ggccgaaact gcggtgacca
     7081 tctgttcttg gccccgggcc ggggccgaaa ctgctcaccg cagatatcct gtttggccca
     7141 acgttagcta ttttcatgta cccgcccttg atctgaactt ctctattctt ggtttggtat
     7201 ttttccatgc cttgcaaaat ggcgttactg cagctagctt gccaaaccta caggtggggt
     7261 ctttcattcc cccctttttc tggagactaa ataaaatctt ttattttatc ggggctgcag
     7321 ggaattcgat atcaagctta tcgatccaaa ctggatctct gctgtccctg taataaaccc
     7381 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     7441 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
     7501 tgtggatgaa tactgccatt tggcgatacc gtcgagatcc gttcactaat cgaatggatc
     7561 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     7621 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     7681 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     7741 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     7801 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     7861 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     7921 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     7981 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     8041 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     8101 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     8161 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     8221 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     8281 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     8341 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc
     8401 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     8461 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     8521 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     8581 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     8641 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     8701 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     8761 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     8821 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     8881 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     8941 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     9001 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     9061 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta
     9121 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa
     9181 gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg
     9241 atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac
     9301 ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc
     9361 tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc
     9421 aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta
     9481 gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag
     9541 cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa
     9601 ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa
     9661 tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc
     9721 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     9781 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     9841 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     9901 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta
     9961 gtcaaggcac tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag
    10021 gtacacaatt tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga
    10081 aaaaacagta tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt
    10141 tccataattt tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa
    10201 gcaagagttc tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag
    10261 tccttggggt cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca
    10321 gtagcctcat catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag
    10381 gcattccacc actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca
    10441 attagaatca gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt
    10501 taaatctctg taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca
    10561 aagatccggg gcccactcat aaatccagtt gccgccacgg tagccaatca ccgtatcgta
    10621 taaatcatcg tcggtacgtt cggcatcgct catcacaata cgtgcctgga cgtcgaggat
    10681 ttcgcgtggg tcaatgccgc gccagatcca catcagacgg ttaatcatgc gataccagtg
    10741 agggatggtt ttaccatcaa gggccgactg cacaggcggt tgtgcgccgt gattaaagcg
    10801 gcggactagc gtcgaggttt caggatgttt aaagcggggt ttgaacaggg tttcgctcag
    10861 gtttgcctgt gtcatggatg cagcctccag aatacttact ggaaactatt gtaacccgcc
    10921 tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg aaaagattag cgaccggaga
    10981 ttggcgggac gaatacgacg cccatatccc acggctgttc aatccaggta tcttgcggga
    11041 tatcaacaac atagtcatca accagcggac gaccagccgg ttttgcgaag atggtgacaa
    11101 agtgcgcttt tggatacat
//
