LOCUS       sequence-492632-       11944 bp    DNA     circular SYN 12-NOV-2025
DEFINITION  The Oskar germ granule protein tagged with optogenetic and 
            chemogenetic tags for recruitment of desired cargo. Used to 
            demonstrate condensate dissolution using the OptoMBP system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Brumbaugh-Reed EH, Aoki K, Toettcher JE
  TITLE     Rapid and reversible dissolution of biomolecular condensates using 
            light-controlled recruitment of a solubility tag
  JOURNAL   bioRxiv 2024.01.16.575860
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.16.575860"
COMMENT     Sequence Label: pHR Myc-Oskar-FusionRed-FRB-iLID
FEATURES             Location/Qualifiers
     source          1..11944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(157..175)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(158..175)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(465..484)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(698..717)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     817..839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(877..895)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        963..1067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1068..1136,1137..1928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1286..1305)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2099..2687
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2588..2607
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2841..2858
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2928..2948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2933..3262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3113..3248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4396..4461
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4591..4611
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5036..5170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5073..5092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5127..5146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5338..5971
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6018..6143
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6640..6873
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7058..7102
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7387..7504
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7658..8065
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8117..8146
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9563..10258
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             10283..10561
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             10604..11041
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     misc_feature    11145..11733
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11198..11218)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11616..11627)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11736..11752)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11737..11753)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             join(11843..11944,1..132)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
       61 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      121 aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat
      181 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
      241 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
      301 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
      361 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
      421 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
      481 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
      541 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
      601 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
      661 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
      721 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      781 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      841 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      901 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      961 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1021 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1081 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1141 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1201 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1261 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1321 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1381 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1441 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1501 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1561 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1621 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1681 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1741 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1801 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1861 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1921 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1981 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2041 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2221 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2881 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     2941 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3001 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3061 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3121 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3181 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3241 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     3301 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     3361 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     3421 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     3481 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     3541 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     3601 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     3661 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     3721 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     3781 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     3841 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     3901 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     3961 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     4021 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     4081 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     4141 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     4201 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     4261 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     4321 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     4381 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     4441 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     4501 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     4561 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     4621 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     4681 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     4741 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     4801 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     4861 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     4921 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     4981 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5161 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     5221 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     5281 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     5341 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     5401 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     5461 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     5521 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     5581 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     5641 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     5701 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     5761 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     5821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5941 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     6001 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     6061 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     6121 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     6181 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     6241 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     6301 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     6361 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     6421 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     6481 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     6541 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     6601 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     6661 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     6721 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     6781 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     6841 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     6901 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     6961 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7021 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7081 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7141 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7201 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     7261 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     7321 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     7381 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     7441 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     7501 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     7561 attccctgca gccccgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     7621 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
     7681 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
     7741 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
     7801 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     7861 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     7921 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     7981 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     8041 cctccgacag actgagtcgc ccggggggga tctggagctc tcgagaattc tcacgcgtgt
     8101 gatcggagct accatggaac aaaaactcat ctcagaagag gatctgggag gagggtctat
     8161 gaccatcatc gagagcaact atatatccgt gcgcgaggaa tatcccgata tagatagtga
     8221 ggtgcgcgcc atattgctga gccacgccca gaatggaatc acgatatcga gcatcaagag
     8281 tgaatatcga aaactgacgg gcaatccatt tccactgcac gacaacgtga cggatttcct
     8341 gctgaccatt cccaatgtga ccgctgagtg cagcgagtcc ggtaagcgga tcttcaacct
     8401 gaaagcgagc ctgaagaacg gtcacctcct ggatatggtg ctcaaccaga aagagcgcac
     8461 cagcgactac agcagcggag ctccgtccct ggagaacata ccacgagcac ctccacgcta
     8521 ctggaagaat cccttcaaac ggagggctct gtcccagctg aacaccagcc cgaggaccgt
     8581 gcccaagata acggatgaaa agaccaagga tatcgccacc aggccggttt cgctgcatca
     8641 aatggccaat gaggcagcgg agtcgaactg gtgctaccag gataattgga agcatctcaa
     8701 caatttctac cagcaagcca gcgtaaatgc gccaaaaatg ccagtaccca tcaacatcta
     8761 cagccccgat gccccagagg aaccaatcaa tttggctcca cctgggcatc agccaagctg
     8821 cagaacccaa agccagaaaa ccgaaccgac tgaaaaccgc catttgggca tctttgtgca
     8881 tccatttaac ggcatgaaca taatgaagag acgccacgaa atgacgccca cgccaacgat
     8941 tttaaccagt ggaacctaca acgattctct gctgacgatt aactcggatt acgatgccta
     9001 tctgctggac tttccgctta tgggcgatga ttttatgcta tatctcgccc gaatggagct
     9061 aaaatgccga tttaggcgtc acgaacgcgt cctgcagtca ggactttgtg tatccggact
     9121 gacgatcaat ggcgcccgaa atcgtttaaa aagagtccaa ttacccgagg gtactcagat
     9181 catcgtcaat atcggatcgg tggacattat gcgcggcaag cctttggttc agatcgagca
     9241 cgattttcgg ctactgatca aggagatgca caatatgcga ttggtgccga ttctcacaaa
     9301 tcttgcaccg ctgggcaact attgtcacga taaggtgtta tgtgacaaaa tctaccgatt
     9361 caacaagttt atccgaagcg aatgctgtca cctaaaggtc atcgacatac actcctgtct
     9421 aatcaacgaa aggggcgtgg tgcgatttga ttgctttcag gcctcaccac gccaagtcac
     9481 cggttccaag gaaccctatc tgttctggaa caaaatcggt cggcagcgcg tactgcaagt
     9541 tattgaaacg agtctggagt atatggtgag cgagctgatt aaggagaaca tgcccatgaa
     9601 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
     9661 caagccctac gagggcaccc agaccatgag aatcaaggtc gtcgagggcg gccctctccc
     9721 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct tcatcaagca
     9781 ccctccgggc atccccgact tctttaagca gtccttccct gagggcttca catgggagag
     9841 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     9901 cggctgcctc atctacaacg tcaaggttag aggggtgaac ttcccagcca acggccctgt
     9961 gatgcagaag aaaacactcg gctgggaggc ctccaccgag acgatgtacc ccgctgacgg
    10021 cggcctggaa ggcgcatgtg acatggccct gaagctcgtg ggcgggggcc acctgatctg
    10081 caaccttgag accacataca gatccaagaa acccgctacg aacctcaaga tgcccggcgt
    10141 ctacaacgtg gaccacagac tggaaagaat caaggaggcc gacgatgaga cctacgtcga
    10201 gcagcacgag gtggctgtgg ccagatactc tactggtggc gctggtgatg gaggtaaagc
    10261 ggccacgcgt ggaggtagcg ggatcctctg gcatgagatg tggcatgaag gcctggaaga
    10321 ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc
    10381 cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc
    10441 ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa
    10501 tgtcaaggac ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa
    10561 gggaagcgga ggtagtgctg gtggtggtgg atccggtgag tttctggcaa ccacactgga
    10621 acggatcgag aaaaatttcg tgattactga tccgagactg cctgacaacc caatcatttt
    10681 tgcgagcgat tccttcctgc agctgacaga atattctcgg gaagagatcc tggggcgcaa
    10741 ttgccgtttt ctgcagggac ccgagacaga ccgtgccact gttcggaaaa tcagagatgc
    10801 tattgacaac cagactgaag tgaccgttca gctgatcaat tataccaaga gcggcaagaa
    10861 gttctggaac gtgttccacc tgcagccgat gcgcgattat aagggcgacg tccagtactt
    10921 cattggcgtg cagctggatg gcaccgaacg tcttcatggc gccgctgagc gtgaggcggt
    10981 ctgcctgatc aaaaagacag cctttcagat tgctgaggca gcgaacgacg aaaattactt
    11041 ttaataagcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta
    11101 gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt
    11161 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
    11221 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
    11281 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
    11341 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
    11401 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
    11461 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
    11521 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
    11581 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
    11641 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
    11701 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt
    11761 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
    11821 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
    11881 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    11941 taac
//
