LOCUS       sequence-492740-       10090 bp    DNA     circular SYN 13-NOV-2025
DEFINITION  Gateway entry plasmid (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10090)
  TITLE     Enhancing Cas12a and Cas9 mediated deletion sizes with exonucleases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pYPQ166-AtEXO1B-N
FEATURES             Location/Qualifiers
     source          1..10090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4792..4839
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4897..4962
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4969..4989
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5014..9114
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5014..9114
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             9027..9035
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9115..9162
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    9837..9960
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9977..9996)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9978..9996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10001..10017)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10001..10017)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggtacgttcc ctaccggccg
     2581 catatgggta tacaagggct tttgccgttg ttgaaatcga taatggttcc gattcatatc
     2641 aaggaactcg agggttgtat cgtcgccgtc gatacatatt catggctaca caaaggggct
     2701 ttatcttgta gccgagaact ctgcaaggga ttacccacca agaggcatat tcaatactgt
     2761 atgcatagag ttaatttact tcgccatcat ggagttaagc ctatcatggt ctttgatgga
     2821 ggtccgttac cgatgaaact agaacaagag aataagcgtg ctaggtccag aaaggaaaat
     2881 cttgctcgtg cattggagca tgaagcaaat ggaaattcct cagctgctta tgagtgctac
     2941 tcaaaggccg tagatatttc accttctatt gcacatgagt tgatacaagt tctgaggcag
     3001 gagaatgttg attacgttgt tgctccttac gaagctgatg cgcagatggc attcttagct
     3061 attactaagc aagttgatgc gattattact gaggattctg atctcatacc ttttggctgc
     3121 ctaagaatca ttttcaaaat ggacaagttt ggacacggtg ttgagtttca agcctccaag
     3181 ctacccaaaa ataaagatct cagtctatca ggattttcaa gccaaatgct tctcgaaatg
     3241 tgcatattaa gtggctgcga ttatttgcag tctcttccag gaatgggact caaaagagca
     3301 catgcactca ttacaaaatt caaaagctat gacagggtaa ttaagcactt aaagtacagc
     3361 acagtttcag ttcctcctct ttatgaagag tcattcaaga gagctttact aactttcaag
     3421 catcaacgtg tctatgaccc aaatgctgaa gatatcatac acttgtgtga catttctgat
     3481 aaccttggtg aagattcaga ttttgtaggc ccatcgatgc cacaagatat tgctaagggt
     3541 atagctctag gccagcttga tccattcaca cagttgccat tccaggctga gagtgttact
     3601 cctaaattgg ctgttgatga tatatcccgt cccaaaagtt tcaaacctga aactgtaaag
     3661 aaaaagcttg atttgccagt ccaaaaaaac cttctcacca agtacttctg ctttgcgtct
     3721 gttgaggcaa aaagaaaatt caaggctcct aggatatcac caatgtctct taccccaact
     3781 gatgagtctc caagcattcc tgatgacaac acaccagatt tagatgctct ttcaagccag
     3841 acaacaaatg aatccccggt ttatagcttg ggtgagaatc catgtgttag cgaagttgca
     3901 gagaaaagag attcacccga tgacgatgca gtggaaagga atcacaagga ccttcatcat
     3961 aaatactgcg aacgggaagt ggataggccg aaatcagata gtttgaaggt tatagtaaga
     4021 agcaagtatt ttaagcagaa acaggaggac aaaagtctga aacaatctat cccatgtctt
     4081 aatgattgct ctgtgattgg tcaaagaaag gctgttaaaa ctgtgattaa catgtcaagc
     4141 gcgtctaaaa gagaagagag tcacagagct atagcaacaa gtccatgttt acatcatgac
     4201 cgaatctaca acgatcatga agatgctaaa gaagcgagtt tctctgccat gaacgaggtg
     4261 gctgagagaa caataaacac ccacaagatt aaccatcaga tcaatgaaga agaacaaaac
     4321 ccatccgttg aaattccttc tgcctttagt actcccgaga atgttatacc tctctcttcc
     4381 attgctattg atagttgcca cggagttgca acagggaaga ggaagcttga ctcagatgaa
     4441 aaccttcaca aggaaaattt gaaatccaag cacatgcgca tggatgaaac tgatactgct
     4501 cttaatgcag aaacaccatt agaaaccgat gatgttgaga agtttggttc gaacatttca
     4561 catatagggc attactcaga aatagcagag aaatcagtgg aaagatttgt ctcagctata
     4621 tcgtctttca aatattctgg gactggctct cgtgctagcg gtctccgtgc ccctctcaaa
     4681 gatatccgca acacttgtcc gtccaaaggg ttatctctta aaccagacat cagcaagttt
     4741 ggctatgcgt caagcaaccg acacatggtg acaaagtcaa ggagaatgtt taaacgtcct
     4801 gctgccacca aaaaggccgg acaggctaag aaaaagaagg agctctcagg atctgagacc
     4861 ccaggcacaa gtgaatcagc tacacctgag tccatggatt acaaggacca cgacggggat
     4921 tacaaggacc acgacattga ttacaaggat gatgatgaca agatggctcc gaagaagaag
     4981 aggaaggttg gcatccacgg ggtgccagct gctgacaaga agtactcgat cggcctcgat
     5041 attgggacta actctgttgg ctgggccgtg atcaccgacg agtacaaggt gccctcaaag
     5101 aagttcaagg tcctgggcaa caccgatcgg cattccatca agaagaatct cattggcgct
     5161 ctcctgttcg acagcggcga gacggctgag gctacgcggc tcaagcgcac cgcccgcagg
     5221 cggtacacgc gcaggaagaa tcgcatctgc tacctgcagg agattttctc caacgagatg
     5281 gcgaaggttg acgattcttt cttccacagg ctggaggagt cattcctcgt ggaggaggat
     5341 aagaagcacg agcggcatcc aatcttcggc aacattgtcg acgaggttgc ctaccacgag
     5401 aagtacccta cgatctacca tctgcggaag aagctcgtgg actccacaga taaggcggac
     5461 ctccgcctga tctacctcgc tctggcccac atgattaagt tcaggggcca tttcctgatc
     5521 gagggggatc tcaacccgga caatagcgat gttgacaagc tgttcatcca gctcgtgcag
     5581 acgtacaacc agctcttcga ggagaacccc attaatgcgt caggcgtcga cgcgaaggct
     5641 atcctgtccg ctaggctctc gaagtctcgg cgcctcgaga acctgatcgc ccagctgccg
     5701 ggcgagaaga agaacggcct gttcgggaat ctcattgcgc tcagcctggg gctcacgccc
     5761 aacttcaagt cgaatttcga tctcgctgag gacgccaagc tgcagctctc caaggacaca
     5821 tacgacgatg acctggataa cctcctggcc cagatcggcg atcagtacgc ggacctgttc
     5881 ctcgctgcca agaatctgtc ggacgccatc ctcctgtctg atattctcag ggtgaacacc
     5941 gagattacga aggctccgct ctcagcctcc atgatcaagc gctacgacga gcaccatcag
     6001 gatctgaccc tcctgaaggc gctggtcagg cagcagctcc ccgagaagta caaggagatc
     6061 ttcttcgatc agtcgaagaa cggctacgct gggtacattg acggcggggc ctctcaggag
     6121 gagttctaca agttcatcaa gccgattctg gagaagatgg acggcacgga ggagctgctg
     6181 gtgaagctca atcgcgagga cctcctgagg aagcagcgga cattcgataa cggcagcatc
     6241 ccacaccaga ttcatctcgg ggagctgcac gctatcctga ggaggcagga ggacttctac
     6301 cctttcctca aggataaccg cgagaagatc gagaagattc tgactttcag gatcccgtac
     6361 tacgtcggcc cactcgctag gggcaactcc cgcttcgctt ggatgacccg caagtcagag
     6421 gagacgatca cgccgtggaa cttcgaggag gtggtcgaca agggcgctag cgctcagtcg
     6481 ttcatcgaga ggatgacgaa tttcgacaag aacctgccaa atgagaaggt gctccctaag
     6541 cactcgctcc tgtacgagta cttcacagtc tacaacgagc tgactaaggt gaagtatgtg
     6601 accgagggca tgaggaagcc ggctttcctg tctggggagc agaagaaggc catcgtggac
     6661 ctcctgttca agaccaaccg gaaggtcacg gttaagcagc tcaaggagga ctacttcaag
     6721 aagattgagt gcttcgattc ggtcgagatc tctggcgttg aggaccgctt caacgcctcc
     6781 ctggggacct accacgatct cctgaagatc attaaggata aggacttcct ggacaacgag
     6841 gagaatgagg atatcctcga ggacattgtg ctgacactca ctctgttcga ggaccgggag
     6901 atgatcgagg agcgcctgaa gacttacgcc catctcttcg atgacaaggt catgaagcag
     6961 ctcaagagga ggaggtacac cggctggggg aggctgagca ggaagctcat caacggcatt
     7021 cgggacaagc agtccgggaa gacgatcctc gacttcctga agagcgatgg cttcgcgaac
     7081 cgcaatttca tgcagctgat tcacgatgac agcctcacat tcaaggagga tatccagaag
     7141 gctcaggtga gcggccaggg ggactcgctg cacgagcata tcgcgaacct cgctggctcg
     7201 ccagctatca agaaggggat tctgcagacc gtgaaggttg tggacgagct ggtgaaggtc
     7261 atgggcaggc acaagcctga gaacatcgtc attgagatgg cccgggagaa tcagaccacg
     7321 cagaagggcc agaagaactc acgcgagagg atgaagagga tcgaggaggg cattaaggag
     7381 ctggggtccc agatcctcaa ggagcacccg gtggagaaca cgcagctgca gaatgagaag
     7441 ctctacctgt actacctcca gaatggccgc gatatgtatg tggaccagga gctggatatt
     7501 aacaggctca gcgattacga cgtcgatcat atcgttccac agtcattcct gaaggatgac
     7561 tccattgaca acaaggtcct caccaggtcg gacaagaacc ggggcaagtc tgataatgtt
     7621 ccttcagagg aggtcgttaa gaagatgaag aactactggc gccagctcct gaatgccaag
     7681 ctgatcacgc agcggaagtt cgataacctc acaaaggctg agaggggcgg gctctctgag
     7741 ctggacaagg cgggcttcat caagaggcag ctggtcgaga cacggcagat cactaagcac
     7801 gttgcgcaga ttctcgactc acggatgaac actaagtacg atgagaatga caagctgatc
     7861 cgcgaggtga aggtcatcac cctgaagtca aagctcgtct ccgacttcag gaaggatttc
     7921 cagttctaca aggttcggga gatcaacaat taccaccatg cccatgacgc gtacctgaac
     7981 gcggtggtcg gcacagctct gatcaagaag tacccaaagc tcgagagcga gttcgtgtac
     8041 ggggactaca aggtttacga tgtgaggaag atgatcgcca agtcggagca ggagattggc
     8101 aaggctaccg ccaagtactt cttctactct aacattatga atttcttcaa gacagagatc
     8161 actctggcca atggcgagat ccggaagcgc cccctcatcg agacgaacgg cgagacgggg
     8221 gagatcgtgt gggacaaggg cagggatttc gcgaccgtca ggaaggttct ctccatgcca
     8281 caagtgaata tcgtcaagaa gacagaggtc cagactggcg ggttctctaa ggagtcaatt
     8341 ctgcctaagc ggaacagcga caagctcatc gcccgcaaga aggactggga tccgaagaag
     8401 tacggcgggt tcgacagccc cactgtggcc tactcggtcc tggttgtggc gaaggttgag
     8461 aagggcaagt ccaagaagct caagagcgtg aaggagctgc tggggatcac gattatggag
     8521 cgctccagct tcgagaagaa cccgatcgat ttcctggagg cgaagggcta caaggaggtg
     8581 aagaaggacc tgatcattaa gctccccaag tactcactct tcgagctgga gaacggcagg
     8641 aagcggatgc tggcttccgc tggcgagctg cagaagggga acgagctggc tctgccgtcc
     8701 aagtatgtga acttcctcta cctggcctcc cactacgaga agctcaaggg cagccccgag
     8761 gacaacgagc agaagcagct gttcgtcgag cagcacaagc attacctcga cgagatcatt
     8821 gagcagattt ccgagttctc caagcgcgtg atcctggccg acgcgaatct ggataaggtc
     8881 ctctccgcgt acaacaagca ccgcgacaag ccaatcaggg agcaggctga gaatatcatt
     8941 catctcttca ccctgacgaa cctcggcgcc cctgctgctt tcaagtactt cgacacaact
     9001 atcgatcgca agaggtacac aagcactaag gaggtcctgg acgcgaccct catccaccag
     9061 tcgattaccg gcctctacga gacgcgcatc gacctgtctc agctcggggg cgacaagcgg
     9121 ccagcggcga cgaagaaggc ggggcaggcg aagaagaaga agtgagctca gagctttcgt
     9181 tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac
     9241 accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt cttgtaccat
     9301 ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg
     9361 gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata
     9421 ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa
     9481 cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga
     9541 ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc
     9601 agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat
     9661 ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta
     9721 taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt
     9781 atgacttgcc aattgttccg gaactctaga taagcttacc ggaaagggcg aattcgcaac
     9841 tttgtataca aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     9901 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     9961 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
    10021 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
    10081 atgcctcctc
//
