LOCUS       sequence-492977-        9946 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  Helper plasmid with sfGFP gene inserted at gene VIII locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Kusumawardhani, H., Zoppi, F., Avendano, R. et al.
  TITLE     Engineering intercellular communication using M13 phagemid and 
            CRISPR-based gene regulation for multicellular computing in 
            Escherichia coli
  JOURNAL   Nat Commun 16, 3569 (2025)
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            16, 3569 (2025)"
COMMENT     Sequence Label: HP17 sfGFP::P8
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      406..627
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(1119..1213)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1369..1398)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             1609..2841
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             2853..3116
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             3118..3219
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             3216..3314
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             3311..4024
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      4034..4082
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             4087..5361
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             5364..5702
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             5704..6750
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             6728..8008
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      8051..8082
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(8181..8996)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8904..8923
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8988..9007
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(9354..9946,1..358))
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat
       61 agtttgtcca ctggaaaatc ccaaagcctt taaccaaagg attcctgatt tccacagttc
      121 tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt
      181 tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt
      241 tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg
      301 ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac
      361 atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat
      421 aattacatgt ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg
      481 gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt
      541 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
      601 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
      661 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
      721 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
      781 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
      841 atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa
      901 tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg
      961 ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc
     1021 ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca
     1081 tcaacaggct tacccgtctt actgtcccta gtgcttggat tctcaccaat aaaaaacgcc
     1141 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1201 atcaacagga gtccaagcga gctcgtaaac ttggtctgac agctctagct ccggcaaaaa
     1261 aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
     1321 ttgccacctg acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc
     1381 cacccgtgaa ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg
     1441 actccagacg cgaattattt ttgatggcgt tcctattggt taaaaaatga gctgatttaa
     1501 caaaaattta atgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat
     1561 acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg
     1621 ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac
     1681 ctgatagcct ttgtagacct ctcaaaaata gctaccctct ccggcatgaa tttatcagct
     1741 agaacggttg aatatcatat tgatggtgat ttgactgtct ccggcctttc tcaccctttt
     1801 gaatctttac ctacacatta ctcaggcatt gcatttaaaa tatatgaggg ttctaaaaat
     1861 ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag tattacaggg tcataatgtt
     1921 tttggtacaa ccgatttagc tttatgctct gaggctttat tgcttaattt tgctaattct
     1981 ttgccttgcc tgtatgattt attggatgtt aacgctacta ctattagtag aattgatgcc
     2041 accttttcag ctcgcgcccc aaatgaaaat atagctaaac aggttattga ccatttgcga
     2101 aatgtatcta atggtcaaac taaatctact cgttcgcaga attgggaatc aactgttaca
     2161 tggaatgaaa cttccagaca ccgtacttta gttgcatatt taaaacatgt tgagctacag
     2221 caccagattc agcaattaag ctctaagcca tccgcaaaaa tgacctctta tcaaaaggag
     2281 caattaaagg tactctctaa tcctgacctg ttggagtttg cttccggtct ggttcgcttt
     2341 gaagctcgaa ttaaaacgcg atatttgaag tctttcgggc ttcctcttaa tctttttgat
     2401 gcaatccgct ttgcttctga ctataatagt cagggtaaag acctgatttt tgatttatgg
     2461 tcattctcgt tttctgaact gtttaaagca tttgaggggg attcaatgaa tatttatgac
     2521 gattccgcag tattggacgc tatccagtct aaacatttta ctattacccc ctctggcaaa
     2581 acttcttttg caaaagcctc tcgctatttt ggtttttatc gtcgtctggt aaacgagggt
     2641 tatgatagtg ttgctcttac tatgcctcgt aattcctttt ggcgttatgt atctgcatta
     2701 gttgaatgtg gtattcctaa atctcaactg atgaatcttt ctacctgtaa taatgttgtt
     2761 ccgttagttc gttttattaa cgtagatttt tcttcccaac gtcctgactg gtataatgag
     2821 ccagttctta aaatcgcata aggtaattca caatgattaa agttgaaatt aaaccatctc
     2881 aagcccaatt tactactcgt tctggtgttt ctcgtcaggg caagccttat tcactgaatg
     2941 agcagctttg ttacgttgat ttgggtaatg aatatccggt tcttgtcaag attactcttg
     3001 atgaaggtca gccagcctat gcgcctggtc tgtacaccgt tcatctgtcc tctttcaaag
     3061 ttggtcagtt cggttccctt atgattgacc gtctgcgcct cgttccggct aagtaacatg
     3121 gagcaggtcg cggatttcga cacaatttat caggcgatga tacaaatctc cgttgtactt
     3181 tgtttcgcgc ttggtataat cgctgggggt caaagatgag tgttttagtg tattctttcg
     3241 cctctttcgt tttaggttgg tgccttcgta gtggcattac gtattttacc cgtttaatgg
     3301 aaacttcctc atgagaaaag gcgaagaact gtttaccggt gtggttccga ttctggtgga
     3361 actggacggc gatgttaatg gtcataaatt cagtgttcgc ggcgaaggtg aaggcgatgc
     3421 gacgaacggc aaactgaccc tgaaatttat ctgcaccacg ggtaaactgc cggtcccgtg
     3481 gccgacgctg gtgaccacgc tgacctatgg cgttcaatgt tttgcgcgtt acccggatca
     3541 catgaaacag cacgactttt tcaaatcggc catgccggaa ggctatgtgc aggaacgtac
     3601 gattagcttt aaagacgatg gtacgtataa aacccgcgcg gaagtgaaat tcgaaggcga
     3661 taccctggtt aaccgtatcg aactgaaagg tatcgatttc aaagaagacg gcaatattct
     3721 gggtcataaa ctggaatata acttcaattc ccacaacgtg tacatcaccg cggataaaca
     3781 gaaaaacggc attaaagcca atttcaaaat ccgccataat gtggaagatg gtagcgttca
     3841 gctggccgac cactatcagc aaaacacgcc gattggtgat ggcccggtcc tgctgccgga
     3901 caatcactac ctgagtaccc agtccgtgct gtcaaaagat ccgaacgaaa aacgtgacca
     3961 catggtcctg ctggaatttg tgacggctgc gggtatcacc cacggcatgg acgaactgta
     4021 taaataataa taaaccgata caattaaagg ctccttttgg agcctttttt tttggagatt
     4081 ttcaacgtga aaaaattatt attcgcaatt cctttagttg ttcctttcta ttctcactcc
     4141 gctgaaactg ttgaaagttg tttagcaaaa ccccatacag aaaattcatt tactaacgtc
     4201 tggaaagacg acaaaacttt agatcgttac gctaactatg agggctgtct gtggaatgct
     4261 acaggcgttg tagtttgtac tggtgacgaa actcagtgtt acggtacatg ggttcctatt
     4321 gggcttgcta tccctgaaaa tgagggtggt ggctctgagg gtggcggttc tgagggtggc
     4381 ggttctgagg gtggcggtac taaacctcct gagtacggtg atacacctat tccgggctat
     4441 acttatatca accctctcga cggcacttat ccgcctggta ctgagcaaaa ccccgctaat
     4501 cctaatcctt ctcttgagga gtctcagcct cttaatactt tcatgtttca gaataatagg
     4561 ttccgaaata ggcagggggc attaactgtt tatacgggca ctgttactca aggcactgac
     4621 cccgttaaaa cttattacca gtacactcct gtatcatcaa aagccatgta tgacgcttac
     4681 tggaacggta aattcagaga ctgcgctttc cattctggct ttaatgagga tccattcgtt
     4741 tgtgaatatc aaggccaatc gtctgacctg cctcaacctc ctgtcaatgc tggcggcggc
     4801 tctggtggtg gttctggtgg cggctctgag ggtggtggct ctgagggtgg cggttctgag
     4861 ggtggcggct ctgagggagg cggttccggt ggtggctctg gttccggtga ttttgattat
     4921 gaaaagatgg caaacgctaa taagggggct atgaccgaaa atgccgatga aaacgcgcta
     4981 cagtctgacg ctaaaggcaa acttgattct gtcgctactg attacggtgc tgctatcgat
     5041 ggtttcattg gtgacgtttc cggccttgct aatggtaatg gtgctactgg tgattttgct
     5101 ggctctaatt cccaaatggc tcaagtcggt gacggtgata attcaccttt aatgaataat
     5161 ttccgtcaat atttaccttc cctccctcaa tcggttgaat gtcgcccttt tgtctttggc
     5221 gctggtaaac catatgaatt ttctattgat tgtgacaaaa taaacttatt ccgtggtgtc
     5281 tttgcgtttc ttttatatgt tgccaccttt atgtatgtat tttctacgtt tgctaacata
     5341 ctgcgtaata aggagtctta atcatgccag ttcttttggg tattccgtta ttattgcgtt
     5401 tcctcggttt ccttctggta actttgttcg gctatctgct tacttttctt aaaaagggct
     5461 tcggtaagat agctattgct atttcattgt ttcttgctct tattattggg cttaactcaa
     5521 ttcttgtggg ttatctctct gatattagcg ctcaattacc ctctgacttt gttcagggtg
     5581 ttcagttaat tctcccgtct aatgcgcttc cctgttttta tgttattctc tctgtaaagg
     5641 ctgctatttt catttttgac gttaaacaaa aaatcgtttc ttatttggat tgggataaat
     5701 aatatggctg tttattttgt aactggcaaa ttaggctctg gaaagacgct cgttagcgtt
     5761 ggtaagattc aggataaaat tgtagctggg tgcaaaatag caactaatct tgatttaagg
     5821 cttcaaaacc tcccgcaagt cgggaggttc gctaaaacgc ctcgcgttct tagaataccg
     5881 gataagcctt ctatatctga tttgcttgct attgggcgcg gtaatgattc ctacgatgaa
     5941 aataaaaacg gcttgcttgt tctcgatgag tgcggtactt ggtttaatac ccgttcttgg
     6001 aatgataagg aaagacagcc gattattgat tggtttctac atgctcgtaa attaggatgg
     6061 gatattattt ttcttgttca ggacttatct attgttgata aacaggcgcg ttctgcatta
     6121 gctgaacatg ttgtttattg tcgtcgtctg gacagaatta ctttaccttt tgtcggtact
     6181 ttatattctc ttattactgg ctcgaaaatg cctctgccta aattacatgt tggcgttgtt
     6241 aaatatggcg attctcaatt aagccctact gttgagcgtt ggctttatac tggtaagaat
     6301 ttgtataacg catatgatac taaacaggct ttttctagta attatgattc cggtgtttat
     6361 tcttatttaa cgccttattt atcacacggt cggtatttca aaccattaaa tttaggtcag
     6421 aagatgaaat taactaaaat atatttgaaa aagttttctc gcgttctttg tcttgcgatt
     6481 ggatttgcat cagcatttac atatagttat ataacccaac ctaagccgga ggttaaaaag
     6541 gtagtctctc agacctatga ttttgataaa ttcactattg actcttctca gcgtcttaat
     6601 ctaagctatc gctatgtttt caaggattct aagggaaaat taattaatag cgacgattta
     6661 cagaagcaag gttattcact cacatatatt gatttatgta ctgtttccat taaaaaaggt
     6721 aattcaaatg aaattgttaa atgtaattaa ttttgttttc ttgatgtttg tttcatcatc
     6781 ttcttttgct caggtaattg aaatgaataa ttcgcctctg cgcgattttg taacttggta
     6841 ttcaaagcaa tcaggcgaat ccgttattgt ttctcccgat gtaaaaggta ctgttactgt
     6901 atattcatct gacgttaaac ctgaaaatct acgcaatttc tttatttctg ttttacgtgc
     6961 aaataatttt gatatggtag gttctaaccc ttccattatt cagaagtata atccaaacaa
     7021 tcaggattat attgatgaat tgccatcatc tgataatcag gaatatgatg ataattccgc
     7081 tccttctggt ggtttctttg ttccgcaaaa tgataatgtt actcaaactt ttaaaattaa
     7141 taacgttcgg gcaaaggatt taatacgagt tgtcgaattg tttgtaaagt ctaatacttc
     7201 taaatcctca aatgtattat ctattgacgg ctctaatcta ttagttgtta gtgctcctaa
     7261 agatatttta gataaccttc ctcaattcct ttcaactgtt gatttgccaa ctgaccagat
     7321 attgattgag ggtttgatat ttgaggttca gcaaggtgat gctttagatt tttcatttgc
     7381 tgctggctct cagcgtggca ctgttgcagg cggtgttaat actgaccgcc tcacctctgt
     7441 tttatcttct gctggtggtt cgttcggtat ttttaatggc gatgttttag ggctatcagt
     7501 tcgcgcatta aagactaata gccattcaaa aatattgtct gtgccacgta ttcttacgct
     7561 ttcaggtcag aagggttcta tctctgttgg ccagaatgtc ccttttatta ctggtcgtgt
     7621 gactggtgaa tctgccaatg taaataatcc atttcagacg attgagcgtc aaaatgtagg
     7681 tatttccatg agcgtttttc ctgttgcaat ggctggcggt aatattgttc tggatattac
     7741 cagcaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta ctaatcaaag
     7801 aagtattgct acaacggtta atttgcgtga tggacagact cttttactcg gtggcctcac
     7861 tgattataaa aacacttctc aggattctgg cgtaccgttc ctgtctaaaa tccctttaat
     7921 cggcctcctg tttagctccc gctctgattc taacgaggaa agcacgttat acgtgctcgt
     7981 caaagcaacc atagtacgcg ccctgtagca tgcaggagtc actaagggtt agttagttag
     8041 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt
     8101 atcattgggg ctcactcaaa ggcggtaatc agataaaaaa aatccttagc tttcgctaag
     8161 gatgatttct gctagtatta ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     8221 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     8281 aactcaccga ggcagttcca aagaatggca aggtcctggt aacggtctgc gattccgacc
     8341 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     8401 aaatcaccat gagtgacgac tgaatccggt gagaatggca agagcttgtg catttctttc
     8461 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     8521 ccgttattca tgcgtgattg cgcctgagca agacgaaata cacgatcgct gttaaaagga
     8581 caattacaaa caggaatcga atgtaaccgg cgcaggaaca cggccagcgc atcaacaata
     8641 ttttcacctg aatcaggata ttcttctaat acctggaagg ctgttttccc aggaatcgcg
     8701 gtggtgagta accacgcatc atcaggagta cggataaaat gcttgatggt cgggagaggc
     8761 ataaactccg tcagccagtt gagacggacc atctcatctg taacatcatt ggcaacgcta
     8821 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt
     8881 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcgtcc
     8941 atgttggagt ttaagcgcgg acgggagcaa gacgtttccc gttgaatatg gctcataaca
     9001 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     9061 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     9121 acttttgctg agttgaagga tcagctctag tagttacatt gtcgatctgt tcatggtgaa
     9181 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     9241 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
     9301 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     9361 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     9421 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
     9481 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
     9541 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     9601 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     9661 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     9721 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     9781 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     9841 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     9901 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaa
//
