LOCUS       sequence-492993-       10759 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  Multiplexable FUCCI sensor (FUCCIplex). The hTNNT2 promoter drives 
            the expression of the fluorescent sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Di Sante M, Pezzotti M, Zimmermann J, Enrico A, Deschamps J, Balmas 
            E, Becca S, Reali A, Bertero A, Jug F, Pasqualini FS
  TITLE     CALIPERS: Cell cycle-aware live imaging for phenotyping experiments 
            and regeneration studies
  JOURNAL   bioRxiv 2024.12.19.629259
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.19.629259"
COMMENT     Sequence Label: pLV[Exp]-Hygro-hTNNT2-FUUCIplex
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..151
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(417..461)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    646..879
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1372..1497
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1544..1724
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1725..1951)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1979..1999)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1979..1997)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2018..2034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2018..2034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2031..2053)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2042..2058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2066..2072,2073..2090,2091..2096))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2111..2132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2249..2266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2420..3008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2500..2519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3179..3970,3971..4039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3802..3821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4040..4144)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4170..4625)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4307..4328)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4519..4538
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4752..4774
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4766..4783
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4767..4783
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4793..4812
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4793..4811
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4832..4967
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5007..5128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5018..5037)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5072..5091
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5200..5433
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5505..6530)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5505..6530)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6547..7046)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6595..6614)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6988..7011
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    7077..7665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7183..7194
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7592..7612
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    7683..7707
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7711..8040)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(8071..9015)
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             complement(9022..9075)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(9082..9354)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             complement(join(9409..10119,10120..10122,10123..10125))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9451..9472)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9800..9819
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10059..10080
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      10122..10131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(10132..10156)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(10755..10759,1..16))
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tttctataca aagttgatcc gataatcact agtaaaattt tgaatttttg taatttgttt
       61 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
      121 gtacccccca atcccccctt ttcttttaaa agctagcgat accgtcgaga tccgttcact
      181 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
      241 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
      301 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
      361 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
      421 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
      481 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
      541 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
      601 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
      661 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
      721 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
      781 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
      841 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
      901 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
      961 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     1021 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     1081 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     1141 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     1201 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     1261 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     1321 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     1381 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     1441 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     1501 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     1561 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     1621 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     1681 ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat gtatcgagct
     1741 aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc
     1801 agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc caccaatcgg
     1861 catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt taccatgttg
     1921 caagactaca agagtattgc ataagactac attaagcttg cagctccagc ttttgttccc
     1981 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2041 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2101 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2161 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2221 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2581 cctttctctc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3061 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3181 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3241 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3301 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3361 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3421 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3481 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3541 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3601 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3661 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3721 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3781 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3841 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3901 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3961 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4021 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4081 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4141 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     4201 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4261 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     4321 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     4381 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     4441 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4501 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     4561 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4621 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4681 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4741 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     4801 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
     4861 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     4921 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     4981 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
     5041 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5101 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
     5161 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
     5221 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     5281 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     5341 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     5401 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     5461 taagatctac agctgccttg taagtcattg gtcttaaagg taccctattc ctttgccctc
     5521 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     5581 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     5641 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     5701 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct
     5761 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     5821 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     5881 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     5941 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     6001 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     6061 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     6121 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     6181 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     6241 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaattcccc aatgtcaagc
     6301 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     6361 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     6421 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt
     6481 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat ggtggccacg
     6541 tgaggtcgaa aggcccggag atgaggaaga ggagaacagc gcggcagacg tgcgcttttg
     6601 aagcgtgcag aatgccgggc ctccggagga ccttcgggcg cccgccccgc ccctgagccc
     6661 gcccctgagc ccgcccccgg acccacccct tcccagcctc tgagcccaga aagcgaagga
     6721 gcaaagctgc tattggccgc tgccccaaag gcctacccgc ttccattgct cagcggtgct
     6781 gtccatctgc acgagactag tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc
     6841 gagctgcggg gcggggggga acttcctgac taggggagga gtagaaggtg gcgcgaaggg
     6901 gccaccaaag aacggagccg gttggcgcct accggtggat gtggaatgtg tgcgaggcca
     6961 gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg
     7021 ccttgggaaa agcgcctccc ctacccggta gaattcgaac cgcgggaatt cccgatgcgg
     7081 ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg
     7141 ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac
     7201 gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccatggaaa
     7261 ggacgtcagc ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt
     7321 ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc
     7381 gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc
     7441 agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag
     7501 agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc
     7561 atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag
     7621 aataccagtc aatctttcac aaattttgta atccagaggt tgattatcgg aattcgatta
     7681 tcaccacttt gtacaagaaa gctgggttta cagggccttc cgccgcttct cggccacctc
     7741 tttccagtac tggctgctgg ggttttcttt gatcatcagg tcgaagcttt cttgggtcac
     7801 gccgcccagg ttcttgttct cgctgctctc gggcacgatc acgcctgggc tagaggtggt
     7861 gctggtcagg tggtcgttcc ggtgcttccg cttgctcagg ccggcagaca gctcattctc
     7921 ccggcccacc agagatccgc tggcgctggg ctggatcatc ttcagggtcc gcctgggcac
     7981 gctgctgttc ttgatgttct ctttgatctc ttcctgcttc tgcttcatgc tggggttcat
     8041 ttccagaggc cgccagtggc tggggtagcc gctctcaagc gcggtgatcc gcgtcgcgat
     8101 cctttcggcg agccgtttgc agatcgcccg cagctcgaaa cggatgaagc gcggcagata
     8161 atggtgacag acaaccaggc cccacagctt gccgccgacc accagcgaca ccgccagggt
     8221 ggcgcgcacg cccatgtcct tcaggaactg cagatggcac ggcgacatcg agcgcaggaa
     8281 gcagcccgac atgtcgagat cgcgcccggt cagcggcgac agccgcggct ccagcggcac
     8341 cggctgatag gtgacgtcga ccagcacgcg gacgcgctgc cgcacgtaca gctgccgcgc
     8401 catctgcggg acagtcgacg acggatagcg gttgccgaaa taggattcga gcccaggcac
     8461 atggcactcg gagaatacca ggccgtggcc ttgctcatcg aaacgataca ccatcacccg
     8521 gtcgtagccg gtgcactgct gaaacagcag cacggtgtca tcgcacagcg cgcgcagtga
     8581 acccgccgtg cggatccgct ccagcgccgg cgccagcgtg cctgacagat cgatcgacgg
     8641 gccggcacgt tcgagttcga tgatcagccc gccttccgga ggccgatgca tcagaccgca
     8701 gtactccgta gagggattgc cgatccggca gcgcaccgcg accggcatgc cttcggcggt
     8761 gggatcgaga tgcggcagga tcttgatcaa cagatcgccg tcgatctcgg cgagcggaac
     8821 gccgagtacg cttccgagat tcagaaattc cgcggcgttg gcgctggcct ggatgacgcg
     8881 atgatcatgt tcgctgacga ccagaagcgc gccatgcggc tggatcgagc cggcgaggtg
     8941 gatctcttca tgttcgcaat tcgaatgaga ggcggtcccg aatgcggggc tgccagaggc
     9001 atgacctgct accatggatc tggggccggg gttctcctcc acgtcgccgc aggtcagcag
     9061 gctgcccctg ccctcgtcga cgatggtgtc ctggtcctgg gcgctggtca ggtgaggagg
     9121 ctgtccggcg gcaggtgtgc tcttcttgat tttctggcca gggctgggac aggcagggat
     9181 atcgggggcc tcgggggtgc tagggctgga cacctcatcc acggacagcc ggagccgtct
     9241 gcgggcaggg ggcctggcct gatctctgcc aggggcggca ggaggtctgg ctctctttct
     9301 gctgccggag gtggcggagg caggggctct cagggcaggt ctggcaggag agggttccag
     9361 gggcctccaa tggctagggt agccgccgag gctaggcagc atgctgtact tgtacagctc
     9421 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     9481 cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag
     9541 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgcc
     9601 gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc
     9661 ggcggtgata tagacgttgt cgctaaagta gttgtactcc agcttgtgcc ccaggatgtt
     9721 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     9781 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     9841 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     9901 gtagcgggcg aagcactgca cgccccagga cagggtggtc acgagggtgg gccagggcac
     9961 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
    10021 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
    10081 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cagcctgctt
    10141 ttttgtacaa acttgggagg cgtctgctca gtctcagcgg ggactgggtg aggcagagga
    10201 tggagagggc tttaagcagg catgtgggct ggggcctggt gagccagccc tgcggaggga
    10261 ggaatgtgcg acaggggacg ggtggggcag ggggatggcg gtgggggtgg ggggtgttgg
    10321 ctgctatttt ggcaggtgcc agggacaagg ctacaggaac atgtacccca cgccatataa
    10381 gcccatgtgg tcctccagct gctcagataa gctatttaaa accagagcag atatgcaggg
    10441 aacagtcatg caacataaac cagctgtccc tcttgagaat cctgataaag cagaggccag
    10501 caacccaggc ctgggagggc cagctgggag cagggttggg gggcagaagg caacctccaa
    10561 gacactccat aagtctcagc accagaatct tggaaggcag agggcaagag ttatgtgctg
    10621 ctccacttga actgatgctg ggggtaaaga catcttccag gctactggct cctaatggac
    10681 tgagcagcct taggcaggtt gccggctctg ccagccccag tgaggacatc tgcaaggtgg
    10741 gtcttctcca tgaccaact
//
