LOCUS       sequence-493005-        7647 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  Expresses full-length human LGP2 in sf9 insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Lee KY, Craig C, Patel SS
  TITLE     Unraveling blunt-end RNA binding and ATPase-driven translocation 
            activities of the RIG-I family helicase LGP2.
  JOURNAL   Nucleic Acids Res. 2024 Jan 11;52(1):355-369. doi: 
            10.1093/nar/gkad1106.
  PUBMED    38015453
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2024-01-11- 11"; volume: "52"; issue: 
            "1"; pages: "355-369"
COMMENT     Sequence Label: pBacPAK9_WT-LGP2
FEATURES             Location/Qualifiers
     source          1..7647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     159..178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(397..501)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     569..587
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(625..647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     747..766
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(783..1238)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(920..941)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1132..1151
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_recomb     1385..2742
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             1385..2726
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(4817..4837)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(4859..4876)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(4939..5030)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4997..5014)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     5034..5861
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5094..5699
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(5737..5861)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(6253..6270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6424..7012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6504..6523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7183..7647,1..327,328..396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
       61 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
      181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
      361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
      421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      481 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
      541 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
      601 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      661 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      721 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      781 taaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      841 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
      901 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      961 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     1021 caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     1081 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     1141 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     1201 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac tatggttgct ttgacgtatg
     1261 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg acgacagcga
     1321 agtgtctcgc cactacaatg ctgtagtaaa aatgaaaagg cttgttatcg gcgtgcggga
     1381 tcgatccgag cttgagacaa tctctttaca atacaattgc ttacattgag cggttgttaa
     1441 atatcggcac ggtaaacgat agcgaaatta caatgcttat agcagatttt tacgatttgt
     1501 attccaatta taatattgaa ttgccaccac cccaagcgct gcctcgaagc agacgacctt
     1561 ccgttgtgca gccagcggcg cctgcgccgg tgcccacaat cgtgcgcgaa caaactaaac
     1621 cagaacaaat tataccggcg gcaccgccgc caccaccttc tcccgtgcct aacattccag
     1681 cgcctccacc accaccacca ccatcgatgt ctgaattgcc gcccgctcca ccaatgccga
     1741 cggaacctca acccgctgca cctttagacg acagacaaca attgttggaa gctattagaa
     1801 acgaaaaaaa tcgcactcgt ctcagaccgg tcaaaccaaa aacggcgccc gaaaccagta
     1861 caatagttga ggtgccgact gtgttgccta aagagacatt tgagcctaaa ccgccgtctg
     1921 catcaccgcc accacctccg cctccgcctc cgccgccagc cccgcctgcg cctccaccga
     1981 tggtagattt atcatcagct ccaccaccgc cgccattagt agatttgccg tctgaaatgt
     2041 taccaccgcc tgcaccatcg ctttctaacg tgttgtctga attaaaatcg ggcacagtta
     2101 gattgaaacc cgcccaaaaa cgcccgcaat cagaaataat tccaaaaagc tcaactacaa
     2161 atttgatcgc ggacgtgtta gccgacacaa ttaataggcg tcgtgtggct atggcaaaat
     2221 cgtcttcgga agcaacttct aacgacgagg gttgggacga cgacgataat cggcctaata
     2281 aagctaacac gcccgatgtt aaatatgtcc aagctttatt taacgtgttt acgtcgagtc
     2341 aattgtacac taacgacagt gatgaaagaa atacaaaagc gcataatatt ttgaacgacg
     2401 tcgaaccttt attacaaaac aaaacacaaa cgaatatcga caaagctaga ttgctgctac
     2461 aagatttggc aagttttgtg gcgttgagcg aaaatccatt agatagtcca gccatcggtt
     2521 cggaaaaaca acccttgttt gaaactaatc gaaacctatt ttacaaatct attgaggatt
     2581 taatatttaa attcagatat aaagacgctg aaaatcattt gattttcgct ctaacatacc
     2641 accctaaaga ttataaattt aatgaattat taaaatacgt acaacaattg tctgtaaatc
     2701 aacaacgcac agaatctagc gcttaataaa tgtactaata acccggatca attaattaag
     2761 cggccgccct gcaggcctct cagtccaggg agaggtccga caagttctcg gcacaatgct
     2821 gcaggaagtc aaagtcaggc acggagaagg gcacgcggga ccactttttg gcctggatcc
     2881 gcccctgagg ggtctccagc agcatgctgc ggactttgag cactggcagc ttcactgact
     2941 tgtagatcat ctgcagaccc cagacctccc cacagttcct gcagctgatg acacccccag
     3001 gcttccagtc cttgaagact ttgttgatga ccacaggatc cctggagaca ttatagtagt
     3061 tcgagaagtt ggggttcaca ttgacatggt gggtgccctc caccttccgc aggtcgctgc
     3121 catggcccac agccaccatg cagttgatgc agagtagctg cacgtgctcc actgggaact
     3181 gctgccgctg gttctcccgc tgggctgcct gggccgcccg cttggtcaag gctgcctgct
     3241 gcagatcccg gatcttggcc tggtactcgg cctggtccat tttctgcaca gcagccactg
     3301 cctgctccat tagcgtctcc agcgcctcgt tgatcagctc ccgcttcagc tcccggctac
     3361 cttcagttgc tacaaacgcg tatacactct gatcggcccg ggcacggccc ctggcctgga
     3421 ccatggagat ttcattggtc aagagcccat aacgcaccac cacattgcaa tgtgggatgt
     3481 ccagcccctc ctccgccaca ctcgtggcca ccagaaggtt cagggttcca tcttggaact
     3541 tctggatcac ttcttgctgg tccctctggg tcatgtgggt gctctggctg ctgttcccag
     3601 ccccaatcag tagctgggcc cggatgtcca cagtctgcag gccctgttgc tgctggagcc
     3661 agagcaggag ggagtgtgcg ctttggcggg tgcgggtgaa gatgataccc cgagggctgt
     3721 tagagctact gaactgcctt tgcaggatct tttccagcat ctccagtttt ggattctctg
     3781 ggccatgagt tgccaagtgg gccagctcat tcttgcggtc atcgaacagg gccagcagcc
     3841 ggcgctcggc acacaggatc tgggttttag tgacgtgctc cctgtgatag aaatcctgca
     3901 gcgcagccaa ggcatccacg gcgcggacgg tgtcatggat gagcagcgcg tcattgtagc
     3961 gcctcaggtg aagcgcatac acccgttgct cctgaagccc agccaaagcc gcagcctcac
     4021 tcagcttcac cacctgctgc tcatacattt gcgtcccaaa tttccggctc aactcaggca
     4081 tctccaggtg gtcatggatt tggtccatga gcttcttcag caagtcccca aacggatcct
     4141 ggctgcgcct gtggcagagg ttgtactgtt tgcaaggctg ttggctgtgc tcctgcagct
     4201 gggggcagca gttctggggt gacatgatgc accacgtgtc caagttggca cagagctgca
     4261 ggacgtggtt gatggcccca tcgagtttgg aggccccgcc agtgcctggg gaggctgtga
     4321 gacccagcac ctggggtagc ggctgtgccc tctggagttt aagttctagg tactggctca
     4381 tgatgacgtt gtagacggtg tccttgtgcg tgtggtggca ctcatccacc acgatcaggg
     4441 agaagacagt gagctccacg tgctcctcct cctcggggct ggtcagtgcc atctgcagaa
     4501 gctctgctgt gcagatgagc aggtcatggc accgggccag gtggccaaag ccagcacgtg
     4561 gtcccatgtc cccactcagg gttgtcacgg tccagcgtcc atccagcatg cgcctgaact
     4621 cttcaccatg ctgggtcacc aggtgcaccc tgttgaccaa tacaaccacc ttggctccat
     4681 ccacagtctc taggtgccgc ttggccacat aagcagccgc ccgggttttc ccggcacccg
     4741 tgggcagcca gatgatgata ttcttgccct ccagggcagg catgatcacc tcccattggt
     4801 aggaccgaag ctccatgccc tgaaaataca ggttttcggt cgttgggata tcgtaatcgt
     4861 gatggtgatg gtgatggtag tacgacatga gttcgaatct agaagatctg gtaccgagct
     4921 cgaattcccg ggatccgtat ttataggttt ttttattaca aaactgttac gaaaacagta
     4981 aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat atcagcaact
     5041 atatattgat agacatttcc agtttgtgat attagtttgt gcgtctcatt acaatggctg
     5101 ttatttttaa caacaaacaa ctgctcgcag acaatagtat agaaaaggga ggtgaactgt
     5161 ttttgtttaa cggttcgtac aacattttgg aaagttatgt taatccggtg ctgctaaaaa
     5221 atggtgtaat tgaactagaa gaagctgcgt actatgccgg caacatattg tacaaaaccg
     5281 acgatcccaa attcattgat tatataaatt taataattaa agcaacacac tccgaagaac
     5341 taccagaaaa tagcactgtt gtaaattaca gaaaaactat gcgcagcggt actatacacc
     5401 ccattaaaaa agacatatat atttatgaca acaaaaaatt tactctatac gatagataca
     5461 tatatggata cgataataac tatgttaatt tttatgagga gaaaaatgaa aaagagaagg
     5521 aatacgaaga agaagacgac aaggcgtcta gtttatgtga aaataaaatt atattgtcgc
     5581 aaattaactg tgaatcattt gaaaatgatt ttaaatatta cctcagcgat tataactacg
     5641 cgttttcaat tatagataat actacaaatg ttcttgttgc gtttggtttg tatcgttaat
     5701 aaaaaacaaa tttgacattt ataattgttt tattattcaa taattacaaa taggattgag
     5761 acccttgcag ttgccagcaa acggacagag cttgtcgaca gttttcattt tttggcagtt
     5821 tggtggttta tacacatctt gggatttgat taaaagatgc atgacttcac ccacacactt
     5881 ggagtcgtgg ttgtagaaat gcgtcagagc cttgattaaa catcggtttt cacacttggc
     5941 acacgaaacc acgtttgcaa tgtaacagtt tagtataaac ctcttcctgt acataccgcc
     6001 gcgcggtaca ttgttttctt tcaaatcgtt caatattttt tttatacacg gaggcatatt
     6061 aatcttgttg ttcagcatgt cgacgacatt tttctttcct tctacgcatt ctttcataca
     6121 aatgtttctc ttgctgcgtt ttttaaaatt ggaattttta atttcattaa tagtgggcgg
     6181 agccgttctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7621 tccgatcgtt gtcagaagta agttggc
//
