LOCUS       sequence-493031-        8333 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  A CRISPR-based technology to study chromosome-specific aneuploidy by
            targeting human centromeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Bosco N, Goldberg A, Zhao X, Mays JC, Cheng P, Johnson AF, Bianchi 
            JJ, Toscani C, Di Tommaso E, Katsnelson L, Annuar D, Mei S, 
            Faitelson RE, Pesselev IY, Mohamed KS, Mermerian A, 
            Camacho-Hernandez EM, Gionco CA, Manikas J, Tseng YS, Sun Z, Fani S,
            Keegan S, Lippman SM, Fenyo D, Giunta S, Santaguida S, Davoli T
  TITLE     KaryoCreate: A CRISPR-based technology to study chromosome-specific 
            aneuploidy by targeting human centromeres.
  JOURNAL   Cell. 2023 Apr 27;186(9):1985-2001.e19. doi: 
            10.1016/j.cell.2023.03.029. Epub 2023 Apr 18.
  PUBMED    37075754
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.03.029"; journalName: "Cell"; date: 
            "2023-04-27- 27"; volume: "186"; issue: "9"; pages: "1985-2001.e19"
COMMENT     Sequence Label: pLenti-gRNA20-1
FEATURES             Location/Qualifiers
     source          1..8333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    153..278
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             325..505
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(506..732)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(760..780)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(760..778)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(799..815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(799..815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(812..834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    823..839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(847..853,854..871,872..877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    892..913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1030..1047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1201..1789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1281..1300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1960..2751,2752..2820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2583..2602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2821..2925)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2951..3406)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3088..3109)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3300..3319
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3533..3555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3547..3564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3548..3564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3574..3593
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3574..3592
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3613..3748
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3788..3909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3799..3818)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3853..3872
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3987..4220
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4292..4880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4398..4409
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4807..4827
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4896..5495)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4979..4999)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5476..5495
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5508..6686)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5517..6455
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5540..5560)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6825..6942
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(7105..7353)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7163..7182)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7333..7353)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7531..7575)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7760..7993
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
       61 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
      121 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
      181 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
      241 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
      301 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
      361 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
      421 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
      481 agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta aatacaatat
      541 ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc tgttgccttc
      601 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
      661 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagagtattg
      721 cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag ggttaattgc
      781 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      841 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      901 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      961 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1921 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1981 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2041 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2101 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2161 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2221 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2281 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2341 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2401 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2461 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2521 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2581 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2641 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2701 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2761 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2821 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2881 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2941 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3001 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3061 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3121 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3181 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3241 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3301 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3361 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     3421 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3481 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3541 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     3601 ggtacgtccc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     3661 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     3721 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggata gctagagcca
     3781 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3841 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3901 aaacaagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
     3961 aagatgacat gaactactat acgtactgct agagattttc cacactgact aaaagggtct
     4021 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     4081 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     4141 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     4201 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     4261 tgccttgtaa gtcattggtc ttaaagtcga cgcggggagg cggcccaaag ggagatccga
     4321 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     4381 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     4441 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     4501 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     4561 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     4621 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     4681 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     4741 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     4801 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     4861 ttgtaatcca gaggttgatt gtcgacttaa cgcgttcagg caccgggctt gcgggtcatg
     4921 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     4981 tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc
     5041 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     5101 ggcgagactc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     5161 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     5221 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacg
     5281 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     5341 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
     5401 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
     5461 gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg ccgtacgtca cgacacctga
     5521 aatggaagaa aaaaactttg aaccactgtc tgaggcttga gaatgaacca agatccaaac
     5581 tcaaaaaggg caaattccaa ggagaattac atcaagtgcc aagctggcct aacttcagtc
     5641 tccacccact cagtgtgggg aaactccatc gcataaaacc cctcccccca acctaaagac
     5701 gacgtactcc aaaagctcga gaactaatcg aggtgcctgg acggcgcccg gtactccgtg
     5761 gagtcacatg aagcgacggc tgaggacgga aaggcccttt tcctttgtgt gggtgactca
     5821 cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc agggccggga
     5881 agcggccatc tttccgctca cgcaactggt gccgaccggg ccagccttgc cgcccagggc
     5941 ggggcgatac acggcggcgc gaggccaggc accagagcag gccggccagc ttgagactac
     6001 ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg
     6061 gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct
     6121 tggcccgcat ttacaagact atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa
     6181 ttttaaatgg ctagagactt atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt
     6241 cgcacgcggc ggccccagcg cccaggccag gcctcaactc aagcacgagg cgaaggggct
     6301 ccttaagcgc aaggcctcga actctcccac ccacttccaa cccgaagctc gggatcaaga
     6361 atcacgtact gcagccaggt ggaagtaatt caaggcacgc aagggccata acccgtaaag
     6421 aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc aaacccgttg
     6481 cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc accctcggga
     6541 aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag
     6601 gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc
     6661 tctgcccact gacgggcacc ggagccaatt cccactcctt tcaagaccta gaaggtccat
     6721 tagctgcaaa gattcctctc tgtttaaaac tttatccatc tttgcacccg ggccccctcg
     6781 agccggcgcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
     6841 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     6901 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
     6961 atttgtctca agatctagtt acgccaagct taaaaaagca ccgactcggt gccacttttt
     7021 caagttgata acggactagc cttatttaaa cttgctatgc tgtttccagc atagctctta
     7081 aactcgtttt gaaggagcag tttcggtgtt tcgtcctttc cacaagatat ataaagccaa
     7141 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt
     7201 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat
     7261 ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg
     7321 caaatatgaa ggaatcatgg gaaataggcc ctctctgggt cccctcgggg ttgggaggtg
     7381 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     7441 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     7501 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     7561 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     7621 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     7681 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     7741 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     7801 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     7861 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     7921 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     7981 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     8041 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     8101 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg
     8161 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     8221 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     8281 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cag
//
