LOCUS       sequence-493043-       10208 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  General screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, 
            Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, 
            Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, 
            Bock C
  TITLE     Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
  JOURNAL   Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9.
            Epub 2025 Sep 24.
  PUBMED    40993398
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09507-9"; journalName: "Nature"; date: 
            "2025-10"; volume: "646"; issue: "8086"; pages: "963-972"
COMMENT     Sequence Label: pPD0034_CROP-seq-mCherry2-2xMS2
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(157..201)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    386..619
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1112..1237
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1284..1464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1465..1691)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1719..1739)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1719..1737)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1758..1774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1758..1774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1771..1793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1782..1798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1806..1812,1813..1830,1831..1836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1851..1872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1989..2006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2160..2748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2240..2259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2919..3710,3711..3779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3542..3561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3780..3884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3910..4365)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4047..4068)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4259..4278
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4492..4514
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4506..4523
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4507..4523
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4533..4552
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4533..4551
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4572..4706
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4725..4846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4736..4755)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4790..4809
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4924..5104
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_RNA        complement(5201..5219)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(5271..5289)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(6356..6604)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6414..6433)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6584..6604)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    6760..7348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6866..6877
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7275..7295
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     7911..7929
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(8075..8131)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8141..8737)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8221..8241)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8718..8737
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8750..9928)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8759..9697
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8782..8802)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10067..10184
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcaagattc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
       61 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
      121 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
      181 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
      241 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
      301 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
      361 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
      421 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
      481 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
      541 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
      601 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
      661 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
      721 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct
      781 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
      841 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
      901 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
      961 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     1021 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     1081 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     1141 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     1201 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     1261 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     1321 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     1381 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     1441 gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc
     1501 tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
     1561 taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
     1621 tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
     1681 ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg
     1741 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3901 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3961 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4021 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4081 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4141 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4201 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4261 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4321 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     4381 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4441 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4501 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     4561 gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4621 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     4681 ggaacgggcg gagttagggg cgggatagct agagccagac atgataagat acattgatga
     4741 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4801 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcctc tcactctctg
     4861 atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa ctactatacg
     4921 tactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4981 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     5041 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     5101 acccagtaca agcaaaaagc agtgtctcaa gatctagtta cgccaagctt ggtgcgccaa
     5161 gcttaaaaaa gcaccgactc ggtgccactt ggccctgcag acatgggtga tcctcatgtt
     5221 ggccaagttg ataacggact agccttattt taacttgcta ggccctgcag acatgggtga
     5281 tcctcatgtt ggcctagctc taaaacagag acgtcgatgg aatgtataat atgccaggac
     5341 aagacttact aaaattttat ttagaagata agtcatgatt tgctgttcgc ccaagtggaa
     5401 ctgaacctaa actaaaaatt tattttatag gtgttggtga atctgttcaa aacgctaaag
     5461 ttaaagtaga cgaaattatt aaagaattaa aattaaaaat gaatatatag gagaaaaaat
     5521 gaaactaaac aaatatatag atcacacatt attaaaacaa gatgctacga aagctgaaat
     5581 taaacaatta tgtgatgaag caattgaatt tgattttgca acagtttgtg ttaattcata
     5641 ttgaacaagc tattgtaaag aattattaaa aggcacaaat gtaggaataa caaatgttgt
     5701 aggttttcct ctaggtgcat gcacaacagc tacaaaagca ttcgaagttt ctgaagcaat
     5761 taaagatggt gcaacagaaa ttgatatggt attaaatatt ggtgcattaa aagacaaaaa
     5821 ttatgaatta gttttagaag acatgaaagc tgtaaaaaaa gcagctggat cacatgttgt
     5881 taaatgtatt atggaaaatt gtttattaac aaaagaagaa atcatgaaag cttgtgaaat
     5941 agctgttgaa gctggattag aatttgttaa aacatcaaca ggattttcaa aatcaggtgc
     6001 aacatttgaa gatgttaaac taatgaagtc agttgttaaa gacaatgctt tagttaaagc
     6061 agctggtgga gttagaacat ttgaagatgc tcaaaaaatg attgaagcag gagctgaccg
     6121 cttaggaaca agtggtggag tagctattat taaaggtgaa gaaaacaacg cgagttacta
     6181 aaactagcgt ttttttattt tgctcatttt tattaaaagt ttgcaaaaag gaacataaaa
     6241 attctaatta ttgatactaa agttattaaa aagaagattt tggttgattt tataaaggtc
     6301 atagaatata atattttagc atgtgtattt tgtgtgctca tttacaaccg tctccggtgt
     6361 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     6421 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     6481 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
     6541 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     6601 cctctctggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaaatcttgt
     6661 cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga
     6721 tctacagctg ccttgtaagt cattggtctt aaagtcgacg cggggaggcg gcccaaaggg
     6781 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     6841 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     6901 cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg
     6961 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     7021 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     7081 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     7141 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     7201 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     7261 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     7321 cacaaatttt gtaatccaga ggttgattgt cgacttaacg cgttcacttg tacagctcgt
     7381 ccatgccgcc ggtgctgtgc ctgccctcgg ccctctcgta ctgctccacg atggtgtagt
     7441 cctcgttgtg gctcaggatg tccagcttga tgtccacgtt gtaggcgccg ggcagctgca
     7501 cgggcttctt ggccttgtag gtggtcttca cctcggcgtc gtagtggccg ccgtccttca
     7561 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atcctctcgg
     7621 tgctggcctc ccagcccatg gtcctgcact gcatcacggg gccgtcgctg gggaagttgg
     7681 tgcccctcag cttcaccttg tagatgaact cgccgtcctg caggctgctg tcctgggtca
     7741 cggtcaccac gccgccgtcc tcgaagttca tcaccctctc ccagttgaag ccctcgggga
     7801 agctcagctt caggtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttgctgccgt
     7861 acatgaactg ggggctcagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
     7921 tcagcttggc ggtctgggtg ccctcgtagg gcctgccctc gccctcgccc tcgatctcga
     7981 actcgtggcc gttcacgctg ccctccatgt gcaccttgaa cctcatgaac tccttgatga
     8041 tggccatgtt gtcctcctcg cccttgctca ccataggacc ggggttttct tccacgtcgc
     8101 ctgcttgctt taacagagag aagttcgtgg ctccggatcc ggcaccgggc ttgcgggtca
     8161 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     8221 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     8281 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     8341 cgggcgagac tccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     8401 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     8461 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     8521 cggcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     8581 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
     8641 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
     8701 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt ggccgtacgt cacgacacct
     8761 gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa
     8821 actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag
     8881 tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag
     8941 acgacgtact ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc cggtactccg
     9001 tggagtcaca tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact
     9061 cacccgcccg ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg
     9121 gaagcggcca tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg
     9181 gcggggcgat acacggcggc gcgaggccag gcaccagagc aggccggcca gcttgagact
     9241 acccccgtcc gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct
     9301 gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat
     9361 cttggcccgc atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa
     9421 aattttaaat ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga
     9481 ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg
     9541 ctccttaagc gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa
     9601 gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa
     9661 agaggccagg cccgcgggaa ccacacacgg cacttacctg tgttctggcg gcaaacccgt
     9721 tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg
     9781 gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct
     9841 aggcaccgga tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
     9901 gctctgccca ctgacgggca ccggagccaa ttcccactcc tttcaagacc tagaaggtcc
     9961 attagctgca aagattcctc tctgtttaaa actttatcca tctttgcacc cgggccccct
    10021 cgagccggcg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
    10081 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
    10141 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
    10201 ccatttgt
//
