LOCUS       sequence-493045-       10988 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  General screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, 
            Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, 
            Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, 
            Bock C
  TITLE     Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
  JOURNAL   Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9.
            Epub 2025 Sep 24.
  PUBMED    40993398
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09507-9"; journalName: "Nature"; date: 
            "2025-10"; volume: "646"; issue: "8086"; pages: "963-972"
COMMENT     Sequence Label: pPD0009_CROP-seq-mCherry2
FEATURES             Location/Qualifiers
     source          1..10988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    227..344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(525..569)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    754..987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1480..1605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1652..1832
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1833..2059)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2087..2107)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2087..2105)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2126..2142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2126..2142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2139..2161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2150..2166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2174..2180,2181..2198,2199..2204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2219..2240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2357..2374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2528..3116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2608..2627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3287..4078,4079..4147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3910..3929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4148..4252)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4278..4733)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4415..4436)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4627..4646
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4860..4882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4874..4891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4875..4891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4901..4920
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4901..4919
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4940..5075
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5115..5236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5126..5145)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5180..5199
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5314..5494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_RNA        complement(5547..5622)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7504..7752)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7562..7581)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7732..7752)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7908..8496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8014..8025
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8423..8443
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     9059..9077
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(9223..9279)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9289..9885)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9369..9389)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9866..9885
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9898..10988,1..88))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9907..10845
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9930..9950)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aggcaccgga tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
       61 gctctgccca ctgacgggca ccggagccaa ttcccactcc tttcaagacc tagaaggtcc
      121 attagctgca aagattcctc tctgtttaaa actttatcca tctttgcacc cgggccccct
      181 cgagccggcg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
      241 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
      301 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
      361 ccatttgtct caagattctg aaacgataat ggtgaatatc cctgcctaac tctattcact
      421 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
      481 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
      541 tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag
      601 gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga
      661 ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg
      721 caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt
      781 tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga
      841 tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat
      901 aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct
      961 cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact
     1021 cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta
     1081 tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc
     1141 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     1201 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     1261 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     1321 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
     1381 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     1441 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     1501 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     1561 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     1621 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     1681 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     1741 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     1801 caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat
     1861 acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt
     1921 tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc
     1981 tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag
     2041 agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt
     2101 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2341 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4261 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4321 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4381 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4441 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4501 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4561 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4621 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4681 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     4741 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     4801 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     4861 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     4921 cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     4981 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     5041 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct
     5101 agagccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     5161 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     5221 ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
     5281 gaataataag atgacatgaa ctactatacg tactgctaga gattttccac actgactaaa
     5341 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     5401 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     5461 cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc agtgtctcaa
     5521 gatctagtta cgccaagctt aaaaaagcac cgactcggtg ccactttttc aagttgataa
     5581 cggactagcc ttattttaac ttgctatttc tagctctaaa acagagacgt acaaaaaaga
     5641 gcaagaagct aaaaaagatt taaaaattat ttttagcgca gttaatggaa caggaactaa
     5701 atttacccca aaaatattac gtgaatcagg atataacgtt attgaggttg aagagcatgc
     5761 atttgaagat gaaacattta aaaatgttgt aaatccaaat ccagaatttg atcctgcatg
     5821 aaaaataccg cttgaatatg gtattaaaca tgatgcagat attattatta tgaatgaccc
     5881 agatgctgac agatttggaa tggcaataaa acatgatggt cattttgtaa gattagatgg
     5941 aaatcaaaca ggaccaattt taattgattg aaaattatca aatctaaaac gcttaaatag
     6001 cattccaaaa aatccggctc tatattcaag ttttgtaaca agtgatttgg gtgatagaat
     6061 cgctcatgaa aaatatggag ttaatattgt aaaaacttta actggattta aatgaatggg
     6121 tagagaaatt gctaaagaag aagataacgg attaaatttt gtttttgctt atgaagaaag
     6181 ttatggatat gtaattgatg actcagctag agataaagat ggaatacaag cttctatatt
     6241 aatagcagag gctgcttgat tttataaaaa acaaaataaa acattagtag actatttaga
     6301 agatttattt aaagaaatgg gtgcatatta cactttcact ttaaacttga attttaaacc
     6361 agaagaaaag aaattaaaaa ttgaaccatt aatgaaatca ttgagagcaa cacccttaac
     6421 tcaaattgct ggacttaaag ttgttaatgt tgaagactac atcgatggaa tgtataatat
     6481 gccaggacaa gacttactaa aattttattt agaagataag tcatgatttg ctgttcgccc
     6541 aagtggaact gaacctaaac taaaaattta ttttataggt gttggtgaat ctgttcaaaa
     6601 cgctaaagtt aaagtagacg aaattattaa agaattaaaa ttaaaaatga atatatagga
     6661 gaaaaaatga aactaaacaa atatatagat cacacattat taaaacaaga tgctacgaaa
     6721 gctgaaatta aacaattatg tgatgaagca attgaatttg attttgcaac agtttgtgtt
     6781 aattcatatt gaacaagcta ttgtaaagaa ttattaaaag gcacaaatgt aggaataaca
     6841 aatgttgtag gttttcctct aggtgcatgc acaacagcta caaaagcatt cgaagtttct
     6901 gaagcaatta aagatggtgc aacagaaatt gatatggtat taaatattgg tgcattaaaa
     6961 gacaaaaatt atgaattagt tttagaagac atgaaagctg taaaaaaagc agctggatca
     7021 catgttgtta aatgtattat ggaaaattgt ttattaacaa aagaagaaat catgaaagct
     7081 tgtgaaatag ctgttgaagc tggattagaa tttgttaaaa catcaacagg attttcaaaa
     7141 tcaggtgcaa catttgaaga tgttaaacta atgaagtcag ttgttaaaga caatgcttta
     7201 gttaaagcag ctggtggagt tagaacattt gaagatgctc aaaaaatgat tgaagcagga
     7261 gctgaccgct taggaacaag tggtggagta gctattatta aaggtgaaga aaacaacgcg
     7321 agttactaaa actagcgttt ttttattttg ctcattttta ttaaaagttt gcaaaaagga
     7381 acataaaaat tctaattatt gatactaaag ttattaaaaa gaagattttg gttgatttta
     7441 taaaggtcat agaatataat attttagcat gtgtattttg tgtgctcatt tacaaccgtc
     7501 tccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
     7561 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
     7621 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
     7681 aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
     7741 aaataggccc tctctgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
     7801 aatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt
     7861 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa agtcgacgcg gggaggcggc
     7921 ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc
     7981 cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa
     8041 ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaaggaa aggacgatga
     8101 tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg
     8161 ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc
     8221 ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac
     8281 agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa
     8341 ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa
     8401 aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt
     8461 caatctttca caaattttgt aatccagagg ttgattgtcg acttaacgcg ttcacttgta
     8521 cagctcgtcc atgccgccgg tgctgtgcct gccctcggcc ctctcgtact gctccacgat
     8581 ggtgtagtcc tcgttgtggc tcaggatgtc cagcttgatg tccacgttgt aggcgccggg
     8641 cagctgcacg ggcttcttgg ccttgtaggt ggtcttcacc tcggcgtcgt agtggccgcc
     8701 gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat
     8761 cctctcggtg ctggcctccc agcccatggt cctgcactgc atcacggggc cgtcgctggg
     8821 gaagttggtg cccctcagct tcaccttgta gatgaactcg ccgtcctgca ggctgctgtc
     8881 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc accctctccc agttgaagcc
     8941 ctcggggaag ctcagcttca ggtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
     9001 gctgccgtac atgaactggg ggctcaggat gtcccaggcg aagggcaggg ggccgccctt
     9061 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggc ctgccctcgc cctcgccctc
     9121 gatctcgaac tcgtggccgt tcacgctgcc ctccatgtgc accttgaacc tcatgaactc
     9181 cttgatgatg gccatgttgt cctcctcgcc cttgctcacc ataggaccgg ggttttcttc
     9241 cacgtcgcct gcttgcttta acagagagaa gttcgtggct ccggatccgg caccgggctt
     9301 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     9361 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     9421 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     9481 ctggtggtcg ggcgagactc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     9541 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     9601 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     9661 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     9721 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     9781 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     9841 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg ccgtacgtca
     9901 cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga gaatgaacca
     9961 agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc aagctggcct
    10021 aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc cctcccccca
    10081 acctaaagac gacgtactcc aaaagctcga gaactaatcg aggtgcctgg acggcgcccg
    10141 gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt tcctttgtgt
    10201 gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc
    10261 agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg ccagccttgc
    10321 cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagagcag gccggccagc
    10381 ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca
    10441 tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt
    10501 gtgcagatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg tcgcagcagg
    10561 tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg cgcgaaggtg
    10621 ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc aagcacgagg
    10681 cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa cccgaagctc
    10741 gggatcaaga atcacgtact gcagccaggt ggaagtaatt caaggcacgc aagggccata
    10801 acccgtaaag aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc
    10861 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
    10921 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
    10981 ccttctct
//
