LOCUS       sequence-493084-       10008 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  Lentiviral vector that can express HCV NS3p. Expressed off of CMV 
            tet ON promoter with dTomato marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K,
            Tiwari R, Mukherjee A
  TITLE     A programmable genetic platform for engineering noninvasive 
            biosensors
  JOURNAL   bioRxiv 2025.09.07.674790
REFERENCE   2  (bases 1 to 10008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.07.674790"
COMMENT     Sequence Label: pANC75(HCV NS3p-IRES-dTomato)
FEATURES             Location/Qualifiers
     source          1..10008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(52..96)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    281..514
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1007..1132
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1179..1359
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1360..1586)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1614..1634)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1614..1632)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1653..1669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1653..1669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1666..1688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1677..1693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1701..1707,1708..1725,1726..1731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1746..1767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1884..1901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2055..2643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2135..2154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2814..3605,3606..3674))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3437..3456
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3675..3779)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3805..4260)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3942..3963)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4154..4173
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4387..4409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4401..4418
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4402..4418
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4428..4447
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4428..4446
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4467..4602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4642..4763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4653..4672)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4707..4726
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4835..5067
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5158..5961)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5269..5288)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5879..5898
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5975..6474)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6023..6042)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6416..6439
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    6517..7105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6623..6634
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7032..7052
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    7136..7160
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7188..7889)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(7271..7294)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7469..7488)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7738..7756
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(7890..7898,7899..7901,7902..8476)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8063..8082)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     8292..8309
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     misc_feature    9157..9209
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(9238..9262)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(9340..9654)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(9343..9367)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9414..9432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9450..9468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9485..9503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9521..9539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9557..9575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9592..9610
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9628..9646
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    9676..9793
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
       61 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
      121 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
      181 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
      241 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
      301 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
      361 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
      421 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
      481 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
      541 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
      601 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
      661 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
      721 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
      781 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
      841 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
      901 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
      961 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     1021 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     1081 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     1141 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     1201 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1261 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1321 gagctcccag gctcagatct ggtctaacca gagagacccg tttatgtatc gagctaggca
     1381 cttaaataca atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc
     1441 cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc
     1501 acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga
     1561 ctacaagagt attgcataag actacattaa gcttgcagct ccagcttttg ttccctttag
     1621 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1681 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1741 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1801 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1861 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2041 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2281 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2341 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2401 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2461 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2521 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2581 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2641 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2701 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2761 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2821 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2881 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2941 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3001 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3061 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3121 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3181 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3241 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3301 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3361 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3421 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3481 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3541 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3601 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3661 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3721 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3781 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     3841 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3901 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3961 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4021 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4081 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4141 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4201 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4261 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     4321 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     4381 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     4441 tatagggcga attgggtacg tccctaggcc tccaaaaaag cctcctcact acttctggaa
     4501 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     4561 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
     4621 gatagctaga gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4681 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4741 ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat
     4801 aaatactgaa taataagatg acatgaacta ctactgctag agattttcca cactgactaa
     4861 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     4921 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     4981 tcccaggctc agatctggtc taactagaga gacccagtac aagcaaaaag cagatcttgt
     5041 cttcgttggg agtgagctag ccttccagtc cccccttttc ttttaaaaag tggctaagat
     5101 ctacagctgc cttgtaagtc attggtctta aaggtaccgg gccctcgacg gatcccctca
     5161 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     5221 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     5281 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     5341 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     5401 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     5461 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     5521 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     5581 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     5641 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     5701 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     5761 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     5821 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     5881 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     5941 ttgttcaatg gccgatccca tattggctgc aggtcgaaag gcccggagat gaggaagagg
     6001 agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc
     6061 ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc
     6121 ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc
     6181 ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta
     6241 cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta
     6301 ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac
     6361 cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg
     6421 gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga
     6481 attagatcac tagtccggat atctgcagaa ttccaggcgg ggaggcggcc caaagggaga
     6541 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     6601 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     6661 gcagaatcca ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca
     6721 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     6781 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     6841 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     6901 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     6961 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     7021 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     7081 aaattttgta atccagaggt tgattgtcga gcggccgcca ctgtgctgga tatcaaccac
     7141 tttgtacaag aaagctgggt ctagatatct cgagtgcggc cgctctactt gtacagctcg
     7201 tccatgccgt acaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg
     7261 gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc
     7321 agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta gtggccgccg
     7381 tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
     7441 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg
     7501 aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc
     7561 tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     7621 tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg
     7681 gagccgtaca tgaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
     7741 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     7801 atctcgaact cgtggccgtt catggagccc tccatgcgca ccttgaagcg catgaactct
     7861 ttgatgacct cctcgccctt gctcaccatg gttgtggcca tattatcatc gtgtttttca
     7921 aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac
     7981 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
     8041 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
     8101 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
     8161 tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt
     8221 cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt
     8281 caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa
     8341 gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa
     8401 catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg
     8461 gggggaggga gaggggctag ctgacagccg gaggggaact gttatctgta aatacagggg
     8521 acctcatcgt tgtttcaagg ttctctactg gtataaagtc tactgctttt gcgacgccgc
     8581 gtgtgcaaac tgcggcccgg aaaattccta ccgcgtggcc ggccgggcag agcaatgggc
     8641 cgccgcttga gcccttgaga taggaaatcg gcctaggact gagcaagctc cctctactat
     8701 cacccctccg gcgaacaggg attacgtcgg catggcgagt caccagatag aggtctgaac
     8761 tgccgcacgc acaaggtgtg agggatcgcg tgccttgagg agcgggccaa cccacgaggt
     8821 ccttatcgac gttagtatac atctgtatga cgggcccttt gggactcgct attgtgcggg
     8881 tccctgcacc atggtagact gtccagcata ctccgtttat acaggtggca agaaaggtct
     8941 gagccgccgt tgaaacaatc tgaacttctc cctcagcctg gtttttgtct ctgcccgtaa
     9001 gtgaggtaat aatgcacccc agcagaccgc gtgtctgttg ggcgtaggcc gttattgggg
     9061 cggacccaga accagagagc agcaatcgcc ctacaagcac cacagagccg gatcccatgg
     9121 tgaagggtac tggatccgag ctcggtggcg accggtggat cccgggcccg cggtaccgtc
     9181 gactgcagaa ttcgaagctt gagctcgaga tccagtcgac tgaattggtt cctttaaagc
     9241 ctgctttttt gtacaaactt gttgatatct gcagaattcc accacactgg actagtcgac
     9301 gaaccggtgg atccccgggt accgagctcg aattctccag gcgatctgac ggttcactaa
     9361 acgagctctg cttatatagg cctcccaccg tacacgccta cctcgacata cgttctctat
     9421 cactgatagg gagtaaactc gacatacgtt ctctatcact gatagggata aactcgacat
     9481 acgttctcta tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag
     9541 taaactcgac atacgttctc tatcactgat agggagtaaa ctcgacatcg ttctctatca
     9601 ctgataggga gtaaactcga catacgttct ctatcactga tagggagtaa actcgaggct
     9661 agtctcgtga tcgataaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     9721 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     9781 ttttctttta aaagttaacc gataccgtcg agatccgttc actaatcgaa tggatctgtc
     9841 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
     9901 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     9961 aagtacagca aaaactattc ttaaacctac caagcctcct actatcat
//
