LOCUS       sequence-493085-       10015 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  Lentiviral vector that can express hAqp1_degron based sensor for 
            TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K,
            Tiwari R, Mukherjee A
  TITLE     A programmable genetic platform for engineering noninvasive 
            biosensors
  JOURNAL   bioRxiv 2025.09.07.674790
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.07.674790"
COMMENT     Sequence Label: pANC61(FLAG7
            hAqp1-GSG-(TEVcs-GSG)3x-FKBP12deg...
FEATURES             Location/Qualifiers
     source          1..10015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     250..271
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(341..365)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    396..984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(449..469)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(867..878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1117..1350
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1422..1543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1459..1478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1513..1532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1583..1718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1738..1757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1739..1757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1767..1784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1767..1783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1776..1798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1925..2380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2012..2031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2222..2243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2406..2510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2511..2579,2580..3371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2729..2748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3542..4130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4031..4050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4284..4301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4418..4439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4454..4459,4460..4477,4478..4484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4492..4508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4497..4519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4516..4532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4516..4532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4551..4571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4553..4571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4599..4825
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4826..5006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5053..5178
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5671..5904
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6089..6133
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6400..6517
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6567..7745
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6798..7736
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7693..7713
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7900..7923
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8611..8631
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             8641..8661
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             8671..8691
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             8701..9021
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     misc_feature    join(9025..9599,9600..9602,9603..9611)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9192..9209)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9419..9438
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9612..9614,9615..9617,9618..10015,1..316)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9612..9614,9615..9617,9618..10015,1..313)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9657..9678)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9918..9937)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
       61 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
      121 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
      181 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
      241 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
      301 cgagctgtac aagtaaagcg gccgcactcg agatatctag acccagcttt cttgtacaaa
      361 gtggttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
      421 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      481 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      541 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      601 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      661 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
      721 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
      781 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
      841 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
      901 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
      961 tctccctttg ggccgcctcc ccgcctggaa ttctgcagat atccggacta gtgatctaat
     1021 tctaccgggg atccgtcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg
     1081 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac
     1141 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     1201 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1261 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1321 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     1381 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     1441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     1561 tatcccgccc ctaactccgc ccatcccgcc cctaactcag cccagttccg cccattctcc
     1621 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     1681 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     1741 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     1801 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1921 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2341 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     2401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     2761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     4441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     4501 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     4561 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     4621 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     4681 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     4741 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     4801 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     4861 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4921 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4981 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     5041 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     5101 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     5161 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     5221 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     5281 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     5341 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     5401 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     5461 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     5521 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     5581 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     5641 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     5701 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     5761 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     5821 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     5881 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     5941 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     6001 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     6061 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     6121 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     6181 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     6241 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     6301 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     6361 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     6421 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     6481 agaattacaa aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcgg
     6541 ctccggtgga tcacgagact agcctcggct ccggtgcccg tcagtgggca gagcgcacat
     6601 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     6661 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     6721 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     6781 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     6841 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     6901 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     6961 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     7021 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     7081 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     7141 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     7201 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     7261 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     7321 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     7381 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     7441 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     7501 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     7561 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     7621 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     7681 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     7741 cgtgagagct cggatccagt acccttcacc atggccagcg agttcaagaa gaagctcttc
     7801 tggagggcag tggtggccga gttcctggcc acgaccctct ttgtcttcat cagcatcggt
     7861 tctgccctgg gcttcaaata cccggtgggg aacaaccagg actacaagga cgacgacgac
     7921 aagacggcgg tccaggacaa cgtgaaggtg tcgctggcct tcgggctgag catcgccacg
     7981 ctggcgcaga gtgtgggcca catcagcggc gcccacctca acccggctgt cacactgggg
     8041 ctgctgctca gctgccagat cagcatcttc cgtgccctca tgtacatcat cgcccagtgc
     8101 gtgggggcca tcgtcgccac cgccatcctc tcaggcatca cctcctccct gactgggaac
     8161 tcgcttggcc gcaatgacct ggctgatggt gtgaactcgg gccagggcct gggcatcgag
     8221 atcatcggga ccctccagct ggtgctatgc gtgctggcta ctaccgaccg gaggcgccgt
     8281 gaccttggtg gctcagcccc ccttgccatc ggcctctctg tagcccttgg acacctcctg
     8341 gctattgact acactggctg tgggattaac cctgctcggt cctttggctc cgcggtgatc
     8401 acacacaact tcagcaacca ctggattttc tgggtggggc cattcatcgg gggagccctg
     8461 gctgtactca tctacgactt catcctggcc ccacgcagca gtgacctcac agaccgcgtg
     8521 aaggtgtgga ccagcggcca ggtggaggag tatgacctgg atgccgacga catcaactcc
     8581 agggtggaga tgaagcccaa aggatctggc gaaaatcttt atttccaatc tggctcaggt
     8641 gagaatctgt atttccagtc tggttcaggg gagaacctct acttccagtc tgggtctgga
     8701 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
     8761 acctgtgtgg tgcactacac cgggatgctt gaagatggaa agaaagtcga ttcctcccgg
     8821 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
     8881 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
     8941 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
     9001 gtggagcttc taaaaccgga atagcccctc tccctccccc ccccctaacg ttactggccg
     9061 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     9121 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     9181 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     9241 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     9301 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     9361 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     9421 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     9481 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     9541 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     9601 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     9661 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     9721 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     9781 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     9841 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     9901 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     9961 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacgg
//
