LOCUS       sequence-493162-        9613 bp    DNA     circular SYN 18-NOV-2025
DEFINITION  ROSA26 knock-in vector with homology-mediated end 
            joining(HMEJ)-based method. The CAG promoter drives the expression 
            of the FUCCIplex sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Di Sante M, Pezzotti M, Zimmermann J, Enrico A, Deschamps J, Balmas 
            E, Becca S, Reali A, Bertero A, Jug F, Pasqualini FS
  TITLE     CALIPERS: Cell cycle-aware live imaging for phenotyping experiments 
            and regeneration studies
  JOURNAL   bioRxiv 2024.12.19.629259
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.19.629259"
COMMENT     Sequence Label: R26_CAG_BST_FUCCIplex
FEATURES             Location/Qualifiers
     source          1..9613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(554..619,620..657)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      674..683
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             680..1078
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1112..1336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1425..1728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1730..2005
                     /label=chicken beta-actin promoter
     intron          2006..3022
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2946..2968
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3030..3049
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3087..3096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3093..3095,3096..3098,3099..3809)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3138..3159)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3399..3418)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3746..3767
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3864..4136
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             4143..4196
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4203..5147
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             5178..5507
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     polyA_signal    5537..5648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6634..6650)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6634..6650)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6647..6669)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6658..6674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6682..6688,6689..6706,6707..6712))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6727..6748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6865..6882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7036..7624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7116..7135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7795..8586,8587..8655))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8418..8437
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8656..8760)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8828..8846
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8884..8906)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9006..9025
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9219..9241
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9233..9250
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9234..9250
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acacctgatt ggctgtcgaa gctgtgggac cgggcccttg ctactggctc gagtctcaca
       61 tgagcgaaac cactgcgcgg ggcgcggggg tggcggggag gcgggcgttg gtacggtcct
      121 ccccgaggcc gagcgccgca gtgtctggcc ccgcgcccct gcgcaacgtg gcaggaagcg
      181 cgcgctggag gcgggggcgg gctgccggcc gagacttctg gatggcggcg gccgcggctc
      241 cgccccgggt tcccaccgcc tgaagggcga gacaagcccg acctgctaca ggcactcgtg
      301 ggggtggggg aggagcgggg gtcggtccgg ctggtttgtg ggtgggaggc gcttgttctc
      361 caaaaaccgg cgcgagctgc aatcctgagg gagctgcggt ggaggaggtg gagagaaggc
      421 cgcacccttc tgggcagggg gaggggagtg ccgcaatacc tttatgggag ttctctgctg
      481 cctcccgtct tgtaaggacc gccctgggcc tggaagaagc cctccctcct ttcctcctcg
      541 cgtgatcggt acctagggcg cagtagtcca gggtttcctt gatgatgtca tacttatcct
      601 gtcccttttt tttccacagc tcgcggttga ggacaaactc ttcgcggtct ttccagtttc
      661 gaacgggaga tctgccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
      721 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
      781 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
      841 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
      901 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
      961 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     1021 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagg
     1081 ggatcaattc tctagcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1141 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1201 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1261 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1321 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggactagtta ttaatagtaa
     1381 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1441 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     1501 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     1561 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     1621 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     1681 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     1741 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     1801 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggs gcgcgccagg
     1861 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     1921 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     1981 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     2041 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     2101 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     2161 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     2221 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     2281 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     2341 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     2401 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     2461 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     2521 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     2581 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     2641 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     2701 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     2761 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     2821 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2881 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     2941 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     3001 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     3061 ttttggcaaa gaattaattc ggatccgcca ccatggtgag caagggcgag gagctgttca
     3121 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3181 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3241 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc
     3301 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3361 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3421 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3481 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac tttagcgaca
     3541 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     3601 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3661 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     3721 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3781 tcactctcgg catggacgag ctgtacaagt acagcatgct gcctagcctc ggcggctacc
     3841 ctagccattg gaggcccctg gaaccctctc ctgccagacc tgccctgaga gcccctgcct
     3901 ccgccacctc cggcagcaga aagagagcca gacctcctgc cgcccctggc agagatcagg
     3961 ccaggccccc tgcccgcaga cggctccggc tgtccgtgga tgaggtgtcc agccctagca
     4021 cccccgaggc ccccgatatc cctgcctgtc ccagccctgg ccagaaaatc aagaagagca
     4081 cacctgccgc cggacagcct cctcacctga ccagcgccca ggaccaggac accatcgtcg
     4141 acgagggcag gggcagcctg ctgacctgcg gcgacgtgga ggagaacccc ggccccagat
     4201 ccatggtagc aggtcatgcc tctggcagcc ccgcattcgg gaccgcctct cattcgaatt
     4261 gcgaacatga agagatccac ctcgccggct cgatccagcc gcatggcgcg cttctggtcg
     4321 tcagcgaaca tgatcatcgc gtcatccagg ccagcgccaa cgccgcggaa tttctgaatc
     4381 tcggaagcgt actcggcgtt ccgctcgccg agatcgacgg cgatctgttg atcaagatcc
     4441 tgccgcatct cgatcccacc gccgaaggca tgccggtcgc ggtgcgctgc cggatcggca
     4501 atccctctac ggagtactgc ggtctgatgc atcggcctcc ggaaggcggg ctgatcatcg
     4561 aactcgaacg tgccggcccg tcgatcgatc tgtcaggcac gctggcgccg gcgctggagc
     4621 ggatccgcac ggcgggttca ctgcgcgcgc tgtgcgatga caccgtgctg ctgtttcagc
     4681 agtgcaccgg ctacgaccgg gtgatggtgt atcgtttcga tgagcaaggc cacggcctgg
     4741 tattctccga gtgccatgtg cctgggctcg aatcctattt cggcaaccgc tatccgtcgt
     4801 cgactgtccc gcagatggcg cggcagctgt acgtgcggca gcgcgtccgc gtgctggtcg
     4861 acgtcaccta tcagccggtg ccgctggagc cgcggctgtc gccgctgacc gggcgcgatc
     4921 tcgacatgtc gggctgcttc ctgcgctcga tgtcgccgtg ccatctgcag ttcctgaagg
     4981 acatgggcgt gcgcgccacc ctggcggtgt cgctggtggt cggcggcaag ctgtggggcc
     5041 tggttgtctg tcaccattat ctgccgcgct tcatccgttt cgagctgcgg gcgatctgca
     5101 aacggctcgc cgaaaggatc gcgacgcgga tcaccgcgct tgagagcggc taccccagcc
     5161 actggcggcc tctggaaatg aaccccagca tgaagcagaa gcaggaagag atcaaagaga
     5221 acatcaagaa cagcagcgtg cccaggcgga ccctgaagat gatccagccc agcgccagcg
     5281 gatctctggt gggccgggag aatgagctgt ctgccggcct gagcaagcgg aagcaccgga
     5341 acgaccacct gaccagcacc acctctagcc caggcgtgat cgtgcccgag agcagcgaga
     5401 acaagaacct gggcggcgtg acccaagaaa gcttcgacct gatgatcaaa gaaaacccca
     5461 gcagccagta ctggaaagag gtggccgaga agcggcggaa ggccctgtaa acgcgtagct
     5521 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5581 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5641 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5701 agcaaggggg aggattggga agagaatagc aggcatgctg ggggtcgacg cttctcgatt
     5761 atgggcggga ttcttttgcc taggcttaag gggctaactt ggtccctggg cgttgccctg
     5821 caggggagtg agcagctgta agatttgagg ggcgactccg attagtttat cttcccacgg
     5881 actagagttg gtgtcgaggt tattgtaata agggtggggt agggaaatgg agcttagtca
     5941 ttcacctggg gctgatttta tgcaacgaga ctgcggatta tcactaatca tttttggagc
     6001 atttttctag agacagacat aaagcatgat cacctgagtt ttataccatt tgagaccctt
     6061 gctgcaccac caaagtgtag catcaggtta aatcttaata gaaaaatttt agcttttgct
     6121 tgagaaacca gtgcttccct ccctcaccct ctctccccag gctctctacc cctttgcatc
     6181 cctaccaggc atcttagcaa ctctcactca tacttgatcc cattttccat ttgttgtact
     6241 tgctcctcca gtattcagac atagcactag ctttctccct ctcttgatct tgggtagcct
     6301 ggtgtctcgc gaaaccagac agattggttc caccacaaat taaggcttga gctggggctt
     6361 gactcttacc cagcagtgct tttattcctc cctagttcac gttcttaaat gtttatcttg
     6421 attttcattt tatccttttt ccttagctgg gattctgtcc ctgaccgtct tcacagtcca
     6481 ggtgatcttg actactgctt tacagagaat tggatctgag gttaggcaac atctcccttt
     6541 ttcttcctct aaatacctct catttctgtt cttaccagtt agtaactgat ctcagatgcc
     6601 tgtgtgatag cttccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6661 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6721 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7441 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7741 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7801 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7861 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7921 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7981 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8041 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8101 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8161 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8221 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8281 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8341 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8401 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8461 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8521 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8581 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8641 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8701 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8761 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8821 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     8881 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8941 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     9001 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9061 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     9121 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     9181 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     9241 gacggccagt gaattggctc gaaaccggac ggagccattg ctctcgcaga gggaggagcg
     9301 cttccggcta gcctcttgtc gccgattggc cgtttctcct cccgccgtgt gtgaaaacac
     9361 aaatggcgta ttctggttgg agtaaagctc ctgtcagtta cgccgtcggg agtacgcagc
     9421 cgcttagcga ctctcgcgtt gccccctggg tggggcgggt aggtaggtgg ggtgtagaga
     9481 tgctgggtgt gcgggcgcgg ccggcctcct gcggcgggag gggagggtca gtgaaattgg
     9541 ctctggcgcg ggcgtcctcc caccctcccc ttccttcggg ggagtcggtt tacccgccgc
     9601 ctgcttgtct tcg
//
