LOCUS       sequence-493219-       15034 bp    DNA     circular SYN 19-NOV-2025
DEFINITION  Expression of human Parkin fused to mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Tait SW, Oberst A, Quarato G, Milasta S, Haller M, Wang R, Karvela 
            M, Ichim G, Yatim N, Albert ML, Kidd G, Wakefield R, Frase S, 
            Krautwald S, Linkermann A, Green DR
  TITLE     Widespread mitochondrial depletion via mitophagy does not compromise
            necroptosis.
  JOURNAL   Cell Rep. 2013 Nov 27;5(4):878-85. doi: 
            10.1016/j.celrep.2013.10.034. Epub 2013 Nov 21.
  PUBMED    24268776
REFERENCE   2  (bases 1 to 15034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.10.034"; journalName: "Cell Rep"; date: 
            "2013-11-27- 27"; volume: "5"; issue: "4"; pages: "878-85"
COMMENT     Sequence Label: mCherry-Parkin-IRES-zeo
FEATURES             Location/Qualifiers
     source          1..15034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1781..2488
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1926..1944)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2194..2213
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(3977..4551,4552..4554,4555..4563)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4144..4161)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4371..4390
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4564..4938
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             5161..5630
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             5895..6086
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6080..6102)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6188..6328
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6243..6262
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6343..6360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6514..7102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6594..6613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7225..7359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7249..7268)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7303..7322
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7589..8188)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7672..7692)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8169..8188
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8271..8770)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8319..8338)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8712..8735
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             9199..11124
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     CDS             complement(join(13533..14324,14325..14393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14156..14175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14394..14498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatctaagta agtaggatcc atggtgagca agggcgagga
     1801 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     1861 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     1921 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     1981 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     2041 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2101 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     2161 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     2221 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     2281 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     2341 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     2401 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     2461 caccggcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc aagctttgat
     2521 agtgtttgtc aggttcaact ccagccatgg tttcccagtg gaggtcgatt ctgacaccag
     2581 catcttccag ctcaaggagg tggttgctaa gcgacagggg gttccggctg accagttgcg
     2641 tgtgattttc gcagggaagg agctgaggaa tgactggact gtgcagaatt gtgacctgga
     2701 tcagcagagc attgttcaca ttgtgcagag accgtggaga aaaggtcaag aaatgaatgc
     2761 aactggaggc gacgacccca gaaacgcggc gggaggctgt gagcgggagc cccagagctt
     2821 gactcgggtg gacctcagca gctcagtcct cccaggagac tctgtggggc tggctgtcat
     2881 tctgcacact gacagcagga aggactcacc accagctgga agtccagcag gtagatcaat
     2941 ctacaacagc ttttatgtgt attgcaaagg cccctgtcaa agagtgcagc cgggaaaact
     3001 cagggtacag tgcagcacct gcaggcaggc aacgctcacc ttgacccagg gtccatcttg
     3061 ctgggatgat gttttaattc caaaccggat gagtggtgaa tgccaatccc cacactgccc
     3121 tgggactagt gcagaatttt tctttaaatg tggagcacac cccacctctg acaaggaaac
     3181 atcagtagct ttgcacctga tcgcaacaaa tagtcggaac atcacttgca ttacgtgcac
     3241 agacgtcagg agccccgtcc tggttttcca gtgcaactcc cgccacgtga tttgcttaga
     3301 ctgtttccac ttatactgtg tgacaagact caatgatcgg cagtttgtcc acgaccctca
     3361 gcttggctac tccctgcctt gtgtggctgg ctgtcccaac tccttgatta aagagctcca
     3421 tcacttcagg attctgggag aagagcagta caaccggtac cagcagtatg gtgcagagga
     3481 gtgtgtcctg cagatggggg gcgtgttatg cccccgccct ggctgtggag cggggctgct
     3541 gccggagcct gaccagagga aagtcacctg cgaagggggc aatggcctgg gctgtgggtt
     3601 tgccttctgc cgggaatgta aagaagcgta ccatgaaggg gagtgcagtg ccgtatttga
     3661 agcctcagga acaactactc aggcctacag agtcgatgaa agagccgccg agcaggctcg
     3721 ttgggaagca gcctccaaag aaaccatcaa gaaaaccacc aagccctgtc cccgctgcca
     3781 tgtaccagtg gaaaaaaatg gaggctgcat gcacatgaag tgtccgcagc cccagtgcag
     3841 gctcgagtgg tgctggaact gtggctgcga gtggaaccgc gtctgcatgg gggaccactg
     3901 gttcgacgtg taggaattcc tgcaggcctc gagggccggc gcgccgcggc cgctacgtaa
     3961 attccgcccc cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     4021 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     4081 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4141 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     4201 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     4261 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     4321 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     4381 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     4441 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     4501 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     4561 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     4621 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     4681 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     4741 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     4801 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     4861 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     4921 gccgaggagc aggactgacc gacgccgacc aacaccgccg gtccgacgcg gcccgacggg
     4981 tccgaggggg gtcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5041 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     5101 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     5161 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5221 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     5281 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     5341 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     5401 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     5461 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     5521 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     5581 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     5641 gtatcaaaat tagcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     5701 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
     5761 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
     5821 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
     5881 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
     5941 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
     6001 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
     6061 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6121 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6181 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     6241 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6301 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7201 ctagatcctt ttgcggccga attagatcca gacatgataa gatacattga tgagtttgga
     7261 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     7321 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     7381 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     7441 aaatgtggta tggctgatta tgatcctcta gagtcggtgg gcctcggggg cgggtgcggg
     7501 gtcggcgggg ccgccccggg tggcttcggt cggagccatg gggtcgtgcg ctcctttcgg
     7561 tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg
     7621 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
     7681 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
     7741 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
     7801 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
     7861 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
     7921 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
     7981 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
     8041 gcagctcggt gacccgctcg atgtggcggt ccgggtcgac ggtgtggcgc gtggcggggt
     8101 agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg gacgtcgtcg cgggtggcga
     8161 ggcgcaccgt gggcttgtac tcggtcatgg aaggtcgtct ccttgtgagg ggtcaggggc
     8221 gtgggtcagg ggatggtggc ggcaccggtc gtggcggccg acctgcaggt cgaaaggccc
     8281 ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc
     8341 gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc
     8401 ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg
     8461 ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga
     8521 ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg
     8581 gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga
     8641 gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag
     8701 cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc
     8761 tcccctaccc ggtagaatta attctcatgt ttgacagctt atcatcgata agctgatcct
     8821 cacaggccgc acccagcttt tcttccgttg ccccagtagc atctctgtct ggtgaccttg
     8881 aagaggaaga ggaggggtcc cgagaatccc catccctacc gtccagcaaa aagggggacg
     8941 aggaatttga ggcctggctt gaggctcagg acgcaaatct tgaggatgtt cagcgggagt
     9001 tttccgggct gcgagtaatt ggtgatgagg acgaggatgg ttcggaggat ggggaatttt
     9061 cagacctgga tctgtctgac agcgaccatg aaggggatga gggtgggggg gctgttggag
     9121 ggggcaggag tctgcactcc ctgtattcac tgagcgtcgt ctaataaaga tgtctattga
     9181 tctcttttag tgtgaatcat gtctgacgag gggccaggta caggacctgg aaatggccta
     9241 ggagagaagg gagacacatc tggaccagaa ggctccggcg gcagtggacc tcaaagaaga
     9301 gggggtgata accatggacg aggacgggga agaggacgag gacgaggagg cggaagacca
     9361 ggagccccgg gcggctcagg atcagggcca agacatagag atggtgtccg gagaccccaa
     9421 aaacgtccaa gttgcattgg ctgcaaaggg acccacggtg gaacaggagc aggagcagga
     9481 gcgggagggg caggagcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg
     9541 gcaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga
     9601 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca
     9661 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg
     9721 gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga
     9781 ggggcaggag caggaggagg ggcaggagca ggaggggcag gaggggcagg aggggcagga
     9841 gcaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggaggg
     9901 gcaggagcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg aggggcagga
     9961 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg
    10021 gcaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg ggcaggaggg
    10081 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
    10141 ggaggggcag gagcaggagg ggcaggagca ggaggtggag gccggggtcg aggaggcagt
    10201 ggaggccggg gtcgaggagg tagtggaggc cggggtcgag gaggtagtgg aggccgccgg
    10261 ggtagaggac gtgaaagagc caggggggga agtcgtgaaa gagccagggg gagaggtcgt
    10321 ggacgtggag aaaagaggcc caggagtccc agtagtcagt catcatcatc cgggtctcca
    10381 ccgcgcaggc cccctccagg tagaaggcca tttttccacc ctgtagggga agccgattat
    10441 tttgaatacc accaagaagg tggcccagat ggtgagcctg acgtgccccc gggagcgata
    10501 gagcagggcc ccgcagatga cccaggagaa ggcccaagca ctggaccccg gggtcagggt
    10561 gatggaggca ggcgcaaaaa aggagggtgg tttggaaagc atcgtggtca aggaggttcc
    10621 aacccgaaat ttgagaacat tgcagaaggt ttaagagctc tcctggctag gagtcacgta
    10681 gaaaggacta ccgacgaagg aacttgggtc gccggtgtgt tcgtatatgg aggtagtaag
    10741 acctcccttt acaacctaag gcgaggaact gcccttgcta ttccacaatg tcgtcttaca
    10801 ccattgagtc gtctcccctt tggaatggcc cctggacccg gcccacaacc tggcccgcta
    10861 agggagtcca ttgtctgtta tttcatggtc tttttacaaa ctcatatatt tgctgaggtt
    10921 ttgaaggatg cgattaagga ccttgttatg acaaagcccg ctcctacctg caatatcagg
    10981 gtgactgtgt gcagctttga cgatggagta gatttgcctc cctggtttcc acctatggtg
    11041 gaaggggctg ccgcggaggg tgatgacgga gatgacggag atgaaggagg tgatggagat
    11101 gagggtgagg aagggcagga gtgatgtaac ttgttaggag acgccctcaa tcgtattaaa
    11161 agccgtgtat tcccccgcac taaagaataa atccccagta gacatcatgc gtgctgttgg
    11221 tgtatttctg gccatctgtc ttgtcaccat tttcgtcctc ccaacatggg gcaattgggc
    11281 atacccatgt tgtcacgtca ctcagctccg cgctcaacac cttctcgcgt tggaaaacat
    11341 tagcgacatt tacctggtga gcaatcagac atgcgacggc tttagcctgg cctccttaaa
    11401 ttcacctaag aatgggagca accagcagga aaaggacaag cagcgaaaat tcacgccccc
    11461 ttgggaggtg gcggcatatg caaaggatag cactcccact ctactactgg gtatcatatg
    11521 ctgactgtat atgcatgagg atagcatatg ctacccggat acagattagg atagcatata
    11581 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
    11641 ctacccagat ataaattagg atagcatata ctacccagat atagattagg atagcatatg
    11701 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
    11761 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
    11821 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
    11881 ctacccagat atagattagg atagcatatg ctatccagat atttgggtag tatatgctac
    11941 ccatggcaac attagcccac cgtgctctca gcgacctcgt gaatatgagg accaacaacc
    12001 ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc agcaatcgcg
    12061 cccctatctt ggcccgccca cctacttatg caggtattcc ccggggtgcc attagtggtt
    12121 ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta caatcagcca agttattaca
    12181 cccttatttt acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg tgcccccact
    12241 ccacaatttc aaaaaaaaga gtggccactt gtctttgttt atgggcccca ttggcgtgga
    12301 gccccgttta attttcgggg gtgttagaga caaccagtgg agtccgctgc tgtcggcgtc
    12361 cactctcttt ccccttgtta caaatagagt gtaacaacat ggttcacctg tcttggtccc
    12421 tgcctgggac acatcttaat aaccccagta tcatattgca ctaggattat gtgttgccca
    12481 tagccataaa ttcgtgtgag atggacatcc agtctttacg gcttgtcccc accccatgga
    12541 tttctattgt taaagatatt cagaatgttt cattcctaca ctagtattta ttgcccaagg
    12601 ggtttgtgag ggttatattg gtgtcatagc acaatgccac cactgaaccc cccgtccaaa
    12661 ttttattctg ggggcgtcac ctgaaacctt gttttcgagc acctcacata caccttactg
    12721 ttcacaactc agcagttatt ctattagcta aacgaaggag aatgaagaag caggcgaaga
    12781 ttcaggagag ttcactgccc gctccttgat cttcagccac tgcccttgtg actaaaatgg
    12841 ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc tcatggggtg
    12901 ggagatatcg ctgttcctta ggaccctttt actaacccta attcgatagc atatgcttcc
    12961 cgttgggtaa catatgctat tgaattaggg ttagtctgga tagtatatac tactacccgg
    13021 gaagcatatg ctacccgttt agggttaaca agggggcctt ataaacacta ttgctaatgc
    13081 cctcttgagg gtccgcttat cggtagctac acaggcccct ctgattgacg ttggtgtagc
    13141 ctcccgtagt cttcctgggc ccctgggagg tacatgtccc ccagcattgg tgtaagagct
    13201 tcagccaaga gttacacata aaggcaatgt tgtgttgcag tccacagact gcaaagtctg
    13261 ctccaggatg aaagccactc agtgttggca aatgtgcaca tccatttata aggatgtcaa
    13321 ctacagtcag agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc
    13381 cagttggcaa gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg
    13441 gcagagatgt cgtagtcagg tttagttcgt ccggggcggg gatctggccg caaatcaatc
    13501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    13561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    13621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    13681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    13741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    13801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    13861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    13921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    13981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    14041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    14101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    14161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    14221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    14281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    14341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    14401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    14461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    14521 ccagctttgc tcttaggagt ttcctaatac atcccaaact caaatatata aagcatttga
    14581 cttgttctat gccctagggg gcggggggaa gctaagccag ctttttttaa catttaaaat
    14641 gttaattcca ttttaaatgc acagatgttt ttatttcata agggtttcaa tgtgcatgaa
    14701 tgctgcaata ttcctgttac caaagctagt ataaataaaa atagataaac gtggaaatta
    14761 cttagagttt ctgtcattaa cgtttccttc ctcagttgac aacataaatg cgctgctgag
    14821 aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt
    14881 caattagttg atttttattt ttgacatata catgtgaaag accccacctg taggtttggc
    14941 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
    15001 aagttcagat caaggtcagg aacagatgga acag
//
