LOCUS       sequence-493225-        9452 bp    DNA     circular SYN 19-NOV-2025
DEFINITION  Constitutive expression of NLS-tagged mKate2 from EF1-alpha promoter
            (for lentiviral packaging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Van Eyndhoven LC, Kinsler G, Zhang J, O'Farrell A, Abderrahim R, 
            Srivastav A, Triandafillou CG, Greco TM, Zaret KS, Cristea IM, 
            Orzalli MH, Drayman N, Singh A, Raj A
  TITLE     Lineage memory shapes viral resistance barriers in human skin.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 
            10.1101/2025.06.27.661951.
  PUBMED    40666908
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 
            10.1101/2025.06.27.661951."
COMMENT     Sequence Label: pCGT003_nuclear-mKate2
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      85..673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     574..593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     827..844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    961..982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(997..1002,1003..1020,1021..1027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1035..1051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1040..1062
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1059..1075
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1059..1075
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1094..1114
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1096..1114
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1142..1368
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1369..1549
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1596..1721
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2214..2447
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2632..2676
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2907..3117
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2929..3125
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2976..3111
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3147..4166
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4237..4354
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4492..5670
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4723..5661
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5618..5638
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5678..5687
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5690..5710
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5708..6400
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     CDS             6401..6421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6446..7034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6499..6519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6917..6928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7106..7339
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7417..7538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7454..7473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7508..7527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7578..7713
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7733..7752)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7734..7752)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7762..7779)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7762..7778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7771..7793)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7920..8375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8007..8026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8217..8238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8401..8505
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8506..8574,8575..9366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8724..8743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
       61 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      121 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      181 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      241 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      301 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      361 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      421 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      481 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      541 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      601 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      661 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      721 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      781 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      841 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      901 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      961 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1021 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1081 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     1141 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     1201 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     1261 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     1321 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     1381 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1441 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1501 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1561 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1621 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1681 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1741 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1801 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1861 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1921 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1981 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2041 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2101 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2161 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2221 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2281 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2341 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2401 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2461 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2521 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2581 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2641 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2701 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2761 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2821 cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca
     2881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt
     2941 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3061 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3121 gcaaaaagct ttctagaggt accaccatga aaaagcctga actcaccgct acctctgtcg
     3181 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tccgagggcg
     3241 aagaatctcg ggctttcagc ttcgatgtgg gagggcgtgg atatgtcctg cgggtgaata
     3301 gctgcgccga tggtttctac aaagatcgct atgtttatcg gcactttgca tccgccgctc
     3361 tccctattcc cgaagtgctt gacattgggg agttcagcga gagcctgacc tattgcatct
     3421 cccgccgtgc acagggtgtc accttgcaag acctgcctga aaccgaactg cccgctgttc
     3481 tccagcccgt cgccgaggcc atggatgcca tcgctgccgc cgatcttagc cagaccagcg
     3541 ggttcggccc attcggacct caaggaatcg gtcaatacac tacatggcgc gatttcatct
     3601 gcgctattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     3661 cctccgtcgc ccaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     3721 ggcacctcgt gcacgccgat ttcggctcca acaatgtcct gaccgacaat ggccgcataa
     3781 cagccgtcat tgactggagc gaggccatgt tcggggattc ccaatacgag gtcgccaaca
     3841 tcttcttctg gaggccctgg ttggcttgta tggagcagca gacccgctac ttcgagcgga
     3901 ggcatcccga gcttgcagga tctcctcggc tccgggctta tatgctccgc attggtcttg
     3961 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gctcagggtc
     4021 gctgcgacgc aatcgtccgg tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     4081 gaagcgctgc cgtctggacc gatggctgtg tggaagtgct cgccgatagt ggaaacagac
     4141 gccccagcac tcgtcctagg gcaaaggatc tgcagtaatg aggcgcgccg ttaacgaatt
     4201 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     4261 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     4321 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     4381 tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt ttaaacagag
     4441 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     4501 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4561 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4621 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4681 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     4741 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     4801 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     4861 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     4921 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     4981 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     5041 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     5101 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     5161 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     5221 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     5281 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     5341 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     5401 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     5461 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     5521 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     5581 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     5641 agtttttttc ttccatttca ggtgtcgtga ggctagcgcc accatggtgc ctaagaaaaa
     5701 acggaaagtg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     5761 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     5821 ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct tcgacatcct
     5881 ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga
     5941 cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca catacgaaga
     6001 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     6061 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     6121 cggctgggag gcctccaccg agaccctgta ccccgctgac ggcggcctgg aaggcagagc
     6181 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata
     6241 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag
     6301 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
     6361 ggccagatac tgcgacctcc ctagcaaact ggggcacaga cctaagaaaa aacggaaagt
     6421 atgaggatcc acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6481 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6541 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6601 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6661 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6721 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6781 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6841 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6901 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6961 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7021 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     7081 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7141 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7201 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7261 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7321 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     7381 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7441 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7501 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7681 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     7741 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7801 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7861 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     7921 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7981 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8041 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8101 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8161 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8221 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8281 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8341 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     8401 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8461 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8521 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8581 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8641 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8701 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8761 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8821 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8881 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8941 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9001 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9061 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9121 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9181 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9241 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9301 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9361 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9421 taatttaaaa ggatctaggt gaagatcctt tt
//
