LOCUS       sequence-493244-        9148 bp    DNA     circular SYN 19-NOV-2025
DEFINITION  Stable expression of LYSET R7W (isoform 2; R45W in isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Pechincha C, Groessl S, Kalis R, de Almeida M, Zanotti A, Wittmann 
            M, Schneider M, de Campos RP, Rieser S, Brandstetter M, Schleiffer 
            A, Muller-Decker K, Helm D, Jabs S, Haselbach D, Lemberg MK, Zuber 
            J, Palm W
  TITLE     Lysosomal enzyme trafficking factor LYSET enables nutritional usage 
            of extracellular proteins.
  JOURNAL   Science. 2022 Oct 7;378(6615):eabn5637. doi: 
            10.1126/science.abn5637. Epub 2022 Oct 7.
  PUBMED    36074822
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-10-7"; volume: "378(6615):eabn5637. doi"; pages: " 
            10.1126/science.abn5637. Epub 2022 Oct 7"
COMMENT     Sequence Label: pRRL_LYSET_R7W
FEATURES             Location/Qualifiers
     source          1..9148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..59)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        44..373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      224..359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             383..1408
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             383..1408
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    1448..2036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1501..1521)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1919..1930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2123..2356
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2428..2549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2465..2484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2519..2538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2589..2724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2744..2763)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2745..2763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2773..2789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2782..2804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2931..3386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3018..3037)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3228..3249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3412..3516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3517..3585,3586..4377)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3735..3754)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4548..5136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5037..5056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5290..5307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5424..5445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5460..5465,5466..5483,5484..5490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5498..5514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5503..5525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5522..5538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5522..5538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5557..5577
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5559..5577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5605..5831
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5832..6012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6059..6184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6677..6910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7095..7139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7406..7523
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7539..8750
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8587..8606
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 cataattcac tggccgtcga gatccggact agtcctgtgg aatgtgtgtc agttagggtg
       61 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      121 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      181 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
      241 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
      301 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      361 aggcttttgc aaaaagctta ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
      421 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
      481 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
      541 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
      601 gattccggaa gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg
      661 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
      721 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
      781 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
      841 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
      901 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
      961 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     1021 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     1081 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     1141 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     1201 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     1261 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     1321 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     1381 cagcactcgt ccgagggcaa aggaatagca ctagttaacg cgtcgaccag ctagctggcc
     1441 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1501 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1561 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1621 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1681 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1741 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1801 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     1861 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1921 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1981 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     2041 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2101 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     2161 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2221 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2281 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2341 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     2401 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     2461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2521 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     2581 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2641 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2701 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     2761 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2821 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2881 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     2941 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3001 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3061 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3121 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3181 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3241 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3301 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3361 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     3421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4381 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4681 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5221 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5281 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5341 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5401 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5461 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5521 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     5581 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     5641 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     5701 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     5761 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     5821 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5881 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5941 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6001 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     6061 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     6121 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     6181 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     6241 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     6301 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     6361 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     6421 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     6481 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     6541 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6601 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6661 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6721 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6781 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6841 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     6901 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     6961 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     7021 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     7081 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7141 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7201 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7261 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     7321 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     7381 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
     7441 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     7501 aaattacaaa aattcaaaat tttatcgata agcttgatgg cctccgcgcc gggttttggc
     7561 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     7621 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     7681 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     7741 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     7801 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     7861 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     7921 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     7981 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     8041 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     8101 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     8161 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     8221 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     8281 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     8341 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     8401 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     8461 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     8521 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     8581 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     8641 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     8701 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac ggatgatgaa
     8761 cttccgtcag tggatgggat ggattggagt gggattgtat ctgttagcca gtgcagcagc
     8821 attttactat gtttttgaaa tcagtgagac ttacaacagg ctggccttgg aacacattca
     8881 acagcaccct gaggagcccc ttgaaggaac cacatggaca cactccttga aagctcaatt
     8941 actctccttg cctttttggg tgtggacagt tatttttctg gtaccttact tacagatgtt
     9001 tttgttccta tactcttgta caagagctga tcccaaaaca gtgggctact gtatcatccc
     9061 tatatgcttg gcagttattt gcaatcgcca ccaggcattt gtcaaggctt ctaatcagat
     9121 cagcagacta caactgattg acacgtaa
//
