LOCUS       sequence-493372-        8276 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 434, Emission: 
            474.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8276)
  TITLE     Davidson Cyan Fluorescent Proteins
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTurquoise-Integrin-Alpha2-N-18
FEATURES             Location/Qualifiers
     source          1..8276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4156..4165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4162..4164,4165..4167,4168..4881)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4207..4228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4468..4487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4815..4836
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5004..5125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5041..5060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5095..5114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5132..5587)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5269..5290)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5481..5500
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5614..5718
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5720..6077
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5720..5911
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5743..5763)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5928..6063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6112..6906
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6112..6906
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6166..6185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6776..6795
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7094..7113)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7138..7185
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7514..8102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8003..8022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagac cgccatgggg ccagaacgga caggggccgc gccgctgccg
      601 ctgctgctgg tgttagcgct cagtcaaggc attttaaatt gttgtttggc ctacaatgtt
      661 ggtctcccag aagcaaaaat attttccggt ccttcaagtg aacagtttgg ctatgcagtg
      721 cagcagttta taaatccaaa aggcaactgg ttactggttg gttcaccctg gagtggcttt
      781 cctgagaacc gaatgggaga tgtgtataaa tgtcctgttg acctatccac tgccacatgt
      841 gaaaaactaa atttgcaaac ttcaacaagc attccaaatg ttactgagat gaaaaccaac
      901 atgagcctcg gcttgatcct caccaggaac atgggaactg gaggttttct cacatgtggt
      961 cctctgtggg cacagcaatg tgggaatcag tattacacaa cgggtgtgtg ttctgacatc
     1021 agtcctgatt ttcagctctc agccagcttc tcacctgcaa ctcagccctg cccttccctc
     1081 atagatgttg tggttgtgtg tgatgaatca aatagtattt atccttggga tgcagtaaag
     1141 aattttttgg aaaaatttgt acaaggcctg gatataggcc ccacaaagac acaggtgggg
     1201 ttaattcagt atgccaataa tccaagagtt gtgtttaact tgaacacata taaaaccaaa
     1261 gaagaaatga ttgtagcaac atcccagaca tcccaatatg gtggggacct cacaaacaca
     1321 tttggagcaa ttcaatatgc aagaaaatat gcttattcag cagcttctgg tgggcgacga
     1381 agtgctacaa aagtaatggt agttgtaact gacggtgaat cacatgatgg ttcaatgttg
     1441 aaagctgtga ttgatcaatg caaccatgac aatatactga ggtttggcat agcagttctt
     1501 gggtacttaa acagaaacgc ccttgatact aaaaatttaa taaaagaaat aaaagcaatc
     1561 gctagtattc caacagaaag atactttttc aatgtgtctg atgaagcagc tctactagaa
     1621 aaggctggga cattaggaga acaaattttc agcattgaag gtactgttca aggaggagac
     1681 aactttcaga tggaaatgtc acaagtggga ttcagtgcag attactcttc tcaaaatgat
     1741 attctgatgc tgggtgcagt gggagctttt ggctggagtg ggaccattgt ccagaagaca
     1801 tctcatggcc atttgatctt tcctaaacaa gcctttgacc aaattctgca ggacagaaat
     1861 cacagttcat atttaggtta ctctgtggct gcaatttcta ctggagaaag cactcacttt
     1921 gttgctggtg ctcctcgggc aaattatacc ggccagatag tgctatatag tgtgaatgag
     1981 aatggcaata tcacggttat tcaggctcac cgaggtgacc agattggctc ctattttggt
     2041 agtgtgctgt gttcagttga tgtggataaa gacaccatta cagacgtgct cttggtaggt
     2101 gcaccaatgt acatgagtga cctaaagaaa gaggaaggaa gagtctacct gtttactatc
     2161 aaagagggca ttttgggtca gcaccaattt cttgaaggcc ccgagggcat tgaaaacact
     2221 cgatttggtt cagcaattgc agctctttca gacatcaaca tggatggctt taatgatgtg
     2281 attgttggtt caccactaga aaatcagaat tctggagctg tatacattta caatggtcat
     2341 cagggcacta tccgcacaaa gtattcccag aaaatcttgg gatccgatgg agcctttagg
     2401 agccatctcc agtactttgg gaggtccttg gatggctatg gagatttaaa tggggattcc
     2461 atcaccgatg tgtctattgg tgcctttgga caagtggttc aactctggtc acaaagtatt
     2521 gctgatgtag ctatagaagc ttcattcaca ccagaaaaaa tcactttggt caacaagaat
     2581 gctcagataa ttctcaaact ctgcttcagt gcaaagttca gacctactaa gcaaaacaat
     2641 caagtggcca ttgtatataa catcacactt gatgcagatg gattttcatc cagagtaacc
     2701 tccagggggt tatttaaaga aaacaatgaa aggtgcctgc agaagaatat ggtagtaaat
     2761 caagcacaga gttgccccga gcacatcatt tatatacagg agccctctga tgttgtcaac
     2821 tctttggatt tgcgtgtgga catcagtctg gaaaaccctg gcactagccc tgcccttgaa
     2881 gcctattctg agactgccaa ggtcttcagt attcctttcc acaaagactg tggtgaggac
     2941 ggactttgca tttctgatct agtcctagat gtccgacaaa taccagctgc tcaagaacaa
     3001 ccctttattg tcagcaacca aaacaaaagg ttaacatttt cagtaacgct gaaaaataaa
     3061 agggaaagtg catacaacac tggaattgtt gttgattttt cagaaaactt gttttttgca
     3121 tcattctccc tgccggttga tgggacagaa gtaacatgcc aggtggctgc atctcagaag
     3181 tctgttgcct gcgatgtagg ctaccctgct ttaaagagag aacaacaggt gacttttact
     3241 attaactttg acttcaatct tcaaaacctt cagaatcagg cgtctctcag tttccaagcc
     3301 ttaagtgaaa gccaagaaga aaacaaggct gataatttgg tcaacctcaa aattcctctc
     3361 ctgtatgatg ctgaaattca cttaacaaga tctaccaaca taaattttta tgaaatctct
     3421 tcggatggga atgttccttc aatcgtgcac agttttgaag atgttggtcc aaaattcatc
     3481 ttctccctga aggtaacaac aggaagtgtt ccagtaagca tggcaactgt aatcatccac
     3541 atccctcagt ataccaaaga aaagaaccca ctgatgtacc taactggggt gcaaacagac
     3601 aaggctggtg acatcagttg taatgcagat atcaatccac tgaaaatagg acaaacatct
     3661 tcttctgtat ctttcaaaag tgaaaatttc aggcacacca aagaattgaa ctgcagaact
     3721 gcttcctgta gtaatgttac ctgctggttg aaagacgttc acatgaaagg agaatacttt
     3781 gttaatgtga ctaccagaat ttggaacggg acttttgcat catcaacgtt ccagacagta
     3841 cagctaacgg cagctgcaga aatcaacacc tataaccctg agatatatgt gattgaagat
     3901 aacactgtta cgattcccct gatgataatg aaacctgatg agaaagccga agtaccaaca
     3961 ggagttataa taggaagtat aattgctgga atccttttgc tgttagctct ggttgcaatt
     4021 ttatggaagc tcggcttctt caaaagaaaa tatgaaaaga tgaccaaaaa tccagatgag
     4081 attgatgaga ccacagagct cagtagcggc agcgcaggcg gaagcggggt accgcgggcc
     4141 cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     4201 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4261 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4321 ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc
     4381 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4441 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4501 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4561 gacggcaaca tcctggggca caagctggag tacaactaca tcagcgacaa cgtctatatc
     4621 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     4681 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4741 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     4801 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4861 atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
     4921 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     4981 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5101 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     5161 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     5221 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     5281 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     5341 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     5401 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     5461 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     5521 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     5581 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5641 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5701 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     5761 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5821 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5881 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5941 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6001 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6061 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     6121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     6301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     6661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6781 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6841 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6901 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6961 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7021 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     7081 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     7141 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     7201 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     7261 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     7321 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     7381 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7441 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7621 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8161 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     8221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
