LOCUS       sequence-493374-        9974 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable 
            (PreScission Protease).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9974)
  TITLE     Davidovich Lab AEBP Plasmids 2025
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMB.MBP.JARID2
FEATURES             Location/Qualifiers
     source          1..9974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  892..1116
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1183..1716)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1905..1910,1911..1927,1928..1933))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2481..2572
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2497..2514
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2624..3721
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      3578..3587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3695..3718
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3755..3778
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             5041..5049
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7658..7792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7695..7714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7749..7768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8002..8167
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      8357..8812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8444..8463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8654..8675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8839..8943
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8944..9012,9013..9804)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9162..9181)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcagaccag ccgcgtaacc
      841 tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg
      901 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
      961 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
     1021 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
     1081 aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa
     1141 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     1201 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     1261 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     1321 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     1381 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     1441 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     1501 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     1561 gccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     1621 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     1681 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     1741 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     1801 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     1861 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     1921 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     1981 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
     2041 cggactccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
     2101 gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg
     2161 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
     2221 tgttcgccca ggactctagc tatagttccc tgaattcgca tctagaacgc acaaggtcag
     2281 ggcactcatg cgacaatcaa ctcgatgcat gatccgcacc attgtcgagg ggccagcgtc
     2341 aatagtgccg atgaccacag acccggttaa gacatagccg aatggagccg cgccgaccac
     2401 agaatgatac gtctcaaagt gaataaagct ccacacagtc gaacgctagt ggttggctac
     2461 atactccgga atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa
     2521 ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga
     2581 ttattcatac cgtcccacca tcgggcgcgg aagaatgggt accaaaactg aagaaggtaa
     2641 actggtaatc tggattaacg gcgataaagg ctataacggt ttggctgaag tcggtaagaa
     2701 attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa
     2761 attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg
     2821 ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca
     2881 ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta
     2941 cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatttgctgc cgaacccgcc
     3001 aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc
     3061 gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg
     3121 ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc
     3181 tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc
     3241 agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat
     3301 caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt
     3361 actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat
     3421 taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac
     3481 tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc
     3541 ttacgaggaa gagttggcga aagatccacg tattgccgcc accatggaaa acgcccagaa
     3601 aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc
     3661 ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac
     3721 tgccgcaggc accgattacg atatcccaac gaccctggaa gttctgttcc aggggccctc
     3781 cggtatgagc aaggaaagac ccaagaggaa tatcattcag aagaaatacg atgacagtga
     3841 tgggattccg tggtcagaag aacgggtggt acgtaaagtc ctttatttgt ctctgaagga
     3901 gttcaagaac tcccagaaga ggcagcatgc ggaaggcatt gctgggagcc tgaaaactgt
     3961 gaatgggctc cttggtaatg accagtctaa gggattagga ccagcatcag aacagtcaga
     4021 gaatgaaaag gacgatgcat cccaagtgtc ctccactagc aacgatgtta gttcttcaga
     4081 ttttgaagaa gggccgtcga ggaaaaggcc caggctgcaa gcacaaagga agtttgctca
     4141 gtctcagccg aatagtccca gcacaactcc agtaaagata gtggagccat tgctaccccc
     4201 tccagctact cagatatcag acctctctaa aaggaagcct aagacagaag attttcttac
     4261 ctttctctgc cttcgaggtt ctcctgcgct gcccaacagc atggtgtatt ttggaagctc
     4321 tcaggatgag gaggaagtcg aggaggaaga tgatgagaca gaagacgtca aaacagccac
     4381 caacaatgct tcatcttcat gccagtcgac ccccaggaaa ggaaaaaccc acaaacatgt
     4441 tcacaacggg catgttttca atggttccag caggtcaaca cgggagaagg aacctgttca
     4501 aaaacacaaa agcaaagagg ccactcccgc aaaggagaag cacagcgatc accgggctga
     4561 cagccgccgg gagcaggctt cagctaacca ccccgcagcg gccccctcca cgggttcctc
     4621 ggccaagggg cttgctgcca cccatcacca cccccctctg catcggtcgg ctcaggactt
     4681 acggaaacag gtttctaagg taaacggagt cactcgaatg tcatctctgg gtgcaggtgt
     4741 aaccagtgcc aaaaagatgc gcgaggtcag accttcacca tccaaaactg tgaagtacac
     4801 tgccacggtg acgaaggggg ctgtcacata caccaaagcc aagagagaac tggtcaagga
     4861 caccaaaccc aatcaccaca agcccagttc cgctgtcaac cacacaatct cagggaaaac
     4921 tgaaagtagc aatgcaaaaa cccgcaaaca ggtgctatcc ctcggggggg cgtccaagtc
     4981 cactgggccc gccgtcaatg gcctcaaggt cagtggcagg ttgaacccaa agtcatgcac
     5041 taaggaggtg ggggggcggc agctgcggga gggcctgcag ctgcgggagg ggctgcggaa
     5101 ctccaagagg agactggaag aggcacacca ggcggagaag ccgcagtcgc cccccaagaa
     5161 gatgaaaggg gcggctggcc ccgccgaagg ccctggcaag aaggccccgg ccgagagagg
     5221 tctgctgaac ggacacgtga agaaggaagt gccggagcgc agtctggaga ggaatcggcc
     5281 gaagcgggcc acggccggga agagcacgcc aggcagacaa gcacatggca aggcggacag
     5341 cgcctcctgt gaaaatcgtt ctacctcgca accggagtcc gtgcacaagc cgcaggactc
     5401 gggcaaggcc gagaagggcg gcggcaaggc cgggtgggcg gccatggacg agatccccgt
     5461 cctcaggccc tccgccaagg agttccacga tccgctcatc tacatcgagt cggtccgcgc
     5521 tcaggtggag aagttcggga tgtgcagggt gatcccccct ccggactggc ggcccgagtg
     5581 caagctcaac gatgagatgc ggtttgtcac gcagattcag cacatccaca agctgggccg
     5641 gcgctggggc cccaacgtgc agcggctggc ctgcatcaag aagcacctca aatctcaggg
     5701 catcaccatg gacgagctcc cgctcatagg gggctgtgag ctcgacctgg cctgcttttt
     5761 ccggctgatt aatgagatgg gcggcatgca gcaagtgact gacctcaaaa aatggaacaa
     5821 actagcagac atgctgcgca tccccagaac tgcccaggac cggctggcca agctgcagga
     5881 ggcctactgc cagtacctac tctcctacga ctccctgtcc ccagaggagc accggcggct
     5941 ggagaaggag gtgctgatgg agaaggagat cctggagaag cgcaaggggc cgctggaagg
     6001 ccacacagag aacgaccacc acaagttcca ccctctgccc cgcttcgagc ccaagaatgg
     6061 gctcatccac ggcgtggccc ccaggaacgg cttccgcagc aagctcaagg aggtgggcca
     6121 ggcccagttg aagactggcc ggcggcgact cttcgctcag gaaaaagaag tggtcaagga
     6181 agaggaggag gacaaaggcg tcctcaatga cttccacaag tgcatctata agggaaggtc
     6241 tgtttctcta acaacttttt atcgaacagc gaggaatatc atgagcatgt gtttcagcaa
     6301 ggagcctgcc ccagccgaaa tcgagcaaga gtactggagg ctagtggaag agaaggactg
     6361 ccacgtggca gtgcactgcg gcaaggtgga caccaacact cacggcagtg gattcccagt
     6421 aggaaaatca gaaccctttt cgaggcatgg atggaacctc accgtcctcc ccaataacac
     6481 agggtccatc ctgcgtcacc tcggtgctgt gcctggagtg actattccct ggctaaatat
     6541 tggcatggtc ttttctacct catgctggtc acgcgaccaa aatcaccttc catacattga
     6601 ctacttacac actggtgctg actgcatttg gtattgcatt cctgctgagg aggagaacaa
     6661 gctggaagat gtggtccaca ccctgctgca agccaatggc accccagggc tgcagatgct
     6721 ggaaagcaac gtcatgatct ccccggaggt gctgtgcaaa gaggggatca aggtgcacag
     6781 gaccgtgcag cagagtggcc agtttgtcgt ctgcttcccg ggatcgtttg tgtccaaagt
     6841 gtgctgtggg tacagcgtgt ctgaaaccgt gcactttgct accacccagt ggacaagtat
     6901 gggctttgag acagccaagg aaatgaagcg tcgccatata gctaagccat tctccatgga
     6961 gaagttactc taccagattg cacaagcaga agcaaaaaaa gaaaacggtc ccactctcag
     7021 taccatctca gccctcctgg atgagctcag ggatacagag ctgcggcagc gcaggcagct
     7081 gttcgaggct ggcctccact cctccgcacg ctatggcagc cacgatggca gcagcacggt
     7141 ggcggacggg aagaaaaagc ctcgaaagtg gctgcagttg gagacatcag agaggaggtg
     7201 tcagatatgc cagcacctgt gctacctgtc catggtggta caagagaacg aaaacgtcgt
     7261 gttctgtctg gagtgtgctc tgcgccacgt ggagaaacag aagtcctgcc gagggctgaa
     7321 gttgatgtac cgctacgatg aggaacagat tatcagtctg gtcaatcaga tatgcggcaa
     7381 agtgtctggt aaaaacggca gcattgagaa ctgtctcagt aaacccacac caaaaagagg
     7441 tccccgcaag agagcgacag tggacgtgcc cccctcccgt ctgtcagcct ccagttcatc
     7501 caaaagtgct tcgagctcat caggatccta atgagtcgag aagtactaga ggatcataat
     7561 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7621 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     7681 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7741 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     7801 gatatcgcct aggagatccg gggctgagca tgagacggaa atctgctcgt cagtggtgct
     7861 cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca caactaagca
     7921 agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca caacagtcca
     7981 actagtgcac tgcagtacat caaccagata agtgaaatct agttccaaac tattttgtca
     8041 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     8101 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     8161 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     8221 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
     8281 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat
     8341 ggcgaatggg gggagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8401 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8701 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8761 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc
     8821 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8881 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8941 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9001 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9061 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9121 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9181 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9241 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9301 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9361 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9421 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9481 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9541 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9601 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggt
     9661 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9721 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9781 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9841 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9901 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9961 atcaaaggat cttc
//
