LOCUS       sequence-493416-        9101 bp    DNA     circular SYN 20-NOV-2025
DEFINITION  plasmid for PEmax** mRNA IVT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Liu P, Ponnienselvan K, Nyalile T, Oikemus S, Joynt AT, Iyer S, 
            Kelly K, Guo D, Kyawe PP, Vanderleeden E, Redick SD, Huang L, Chen 
            Z, Lee JM, Schiffer CA, Harlan DM, Wang JP, Emerson CP Jr, Lawson 
            ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA
  TITLE     Increasing intracellular dNTP levels improves prime editing 
            efficiency.
  JOURNAL   Nat Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x.
  PUBMED    39322763
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x."
COMMENT     Sequence Label: PEmax_2star_IVT
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    234..255
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(270..275,276..293,294..300)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    308..324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     313..335
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     332..348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     332..348
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      447..456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             462..488
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             528..548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             549..4649
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4707..4727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6828..6848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6858..6884
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(7237..7255)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7323..7427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7428..7496,7497..8288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7646..7665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8459..9047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8948..8967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
       61 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      301 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacac cgtcagatcc
      361 gctagagatc cgcggccgct aatacgactc actataagga gaataaacta gtattcttct
      421 ggtccccaca gactcagaga gaacccgcca ccatggccgc tccggcagct aagaaaaaga
      481 aactggatgg cagcaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc
      541 ggaaagtcga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
      601 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
      661 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
      721 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
      781 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
      841 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
      901 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
      961 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     1021 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     1081 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     1141 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     1201 gcagaaagct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     1261 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     1321 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     1381 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     1441 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     1501 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     1561 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     1621 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     1681 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaagaga gaggacctgc
     1741 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     1801 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     1861 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     1921 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     1981 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     2041 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     2101 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     2161 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     2221 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     2281 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     2341 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     2401 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     2461 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     2521 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     2581 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     2641 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     2701 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     2761 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     2821 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     2881 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     2941 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     3001 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     3061 acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     3121 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     3181 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     3241 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     3301 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     3361 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     3421 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     3481 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     3541 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     3601 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     3661 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     3721 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     3781 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     3841 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     3901 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
     3961 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     4021 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     4081 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     4141 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg
     4201 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     4261 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
     4321 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     4381 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
     4441 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
     4501 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca
     4561 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
     4621 ggatcgacct gtctcagctg ggaggtgact ccggcggaag ctctggtggc agcaagcgga
     4681 ccgccgacgg ctctgaattc gagagcccta agaagaaaag aaaggtgagc ggaggctcta
     4741 gcggcggaag caccctgaac attgaagacg agtatagact gcatgaaaca agcaaggaac
     4801 ccgacgtgtc cctgggctcc acctggctgt ccgactttcc ccaggcctgg gccgagacag
     4861 gaggaatggg cctggccgtg cggcaggcac ccctgatcat ccctctgaag gccacctcta
     4921 cacccgtgag catcaagcag taccctatgt ctcaggaggc cagactgggc atcaagcctc
     4981 acatccagag gctgctggac cagggcatcc tggtgccatg ccagagcccc tggaacacac
     5041 cactgctgcc cgtgaagaag ccaggcacca atgactatag acccgtgcag gatctgagag
     5101 aggtgaacaa gagggtggag gatatccacc ccaccgtgcc caacccttac aatctgctgt
     5161 ccggcctgcc cccttctcac cagtggtata cagtgctgga cctgaaggat gccttctttt
     5221 gtctgagact gcaccctacc agccagccac tgttcgcctt tgagtggagg gaccctgaga
     5281 tgggcatctc tggccagctg acctggacac gcctgcctca gggcttcaag aatagcccaa
     5341 cactgtttaa cgaggccctg caccgcgacc tggcagattt ccggatccag cacccagatc
     5401 tgatcctgct gcagtacatg gacgatctgc tgctggccgc caccagcgag ctggattgcc
     5461 agcagggaac acgcgccctg ctgcagaccc tgggaaacct gggatatagg gcatccgcca
     5521 agaaggccca gatctgtcag aagcaggtga agtacctggg ctatctgctg aaggagggcc
     5581 agagatggct gacagaggcc aggaaggaga cagtgatggg ccagccaaca cccaagaccc
     5641 caagacagct gagggagttc ctgggcaaag caggattttg caggctgttc atcccaggat
     5701 tcgcagagat ggcagcacct ctgtacccac tgaccaagcc gggcaccctg tttaattggg
     5761 gccctgacca gcagaaggcc tatcaggaga tcaagcaggc cctgctgaca gcaccagccc
     5821 tgggcctgcc agacctgacc aagcctttcg agctgtttgt ggatgagaag cagggctacg
     5881 ccaagggcgt gctgacccag aagctgggac catggagacg gcccgtggcc tatctgtcca
     5941 agaagctgga cccagtggca gcaggatggc caccatgcct gaggatggtg gcagcaatcg
     6001 ccgtgctgac aaaggatgcc ggcaagctga ccatgggaca gccactggtc atcaaggcac
     6061 cacacgcagt ggaggccctg gtgaagcagc ctccagatcg ctggctgtct aacgcccgga
     6121 tgacacacta ccaggccctg ctgctggaca ccgatcgcgt gcagtttggc cctgtggtgg
     6181 ccctgaatcc agccaccctg ctgcctctgc cagaggaggg cctgcagcac aactgtctgg
     6241 acatcctggc agaggcacac ggaacaaggc cagacctgac cgatcagccc ctgcctgacg
     6301 ccgatcacac atggtatacc gatggaagct ccctgctgca ggagggccag aggaaggcag
     6361 gagcagcagt gaccacagag acagaagtga tctgggccaa ggccctgcca gcaggcacat
     6421 ccgcccagcg ggccgagctg atcgccctga cccaggccct gaagatggcc gagggcaaga
     6481 agctgaacgt gtacacagac tccagatatg ccttcgccac cgcacacatc cacggagaga
     6541 tctacaggcg ccggtgctgg ctgacctctg agggcaagga gatcaagaac aaggatgaga
     6601 tcctggccct gctgaaggcc ctgtttctgc ccaagcggct gagcatcatc cactgtcctg
     6661 gacaccagaa gggacactcc gccgaggcaa ggggcaatcg gatggccgac caggccgcca
     6721 gaaaggctgc tattactgaa actcccgaca cttccactct gctgattgaa aactcctccc
     6781 cttctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagtctccc aagaagaaga
     6841 ggaaagtcgg ctctggccct gccgctaaga gagtgaagct ggactaatga ctcgagctgg
     6901 tactgcatgc acacaatgct agctgcccct ttcccgtcct gggtaccccg agtctccccc
     6961 gacctcgggt cccaggtatg ctcccacctc cacctgcccc actcaccacc tctgctagtt
     7021 ccagacacct cccaagcacg cagcaatgca gctcaaaacg cttagcctag ccacaccccc
     7081 acgggaaaca gcagtgatta acctttagca ataaacgaaa gtttaactaa gctatactaa
     7141 ccccagggtt ggtcaatttc gtgccagcca caccctggag ctagcaaaaa aaaaaaaaaa
     7201 agagacggtt taaacccgct gatcagcctc gactgtgcct cgtgatacgc ctatttttat
     7261 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7321 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7381 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7441 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7501 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7561 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7621 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7681 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7741 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7801 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7861 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7921 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7981 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8041 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8101 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc ggtatcattg
     8161 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8221 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8281 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8341 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8401 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8461 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8521 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8581 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8641 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8701 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8761 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8821 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8881 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8941 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9001 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9061 cggccttttt acggttcctg gccttttgct ggccttttgc t
//
