LOCUS       sequence-493697-        9620 bp    DNA     circular SYN 21-NOV-2025
DEFINITION  IVT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, 
            Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, 
            Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, 
            Bock C
  TITLE     Systematic discovery of CRISPR-boosted CAR T cell immunotherapies.
  JOURNAL   Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9.
            Epub 2025 Sep 24.
  PUBMED    40993398
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 21, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09507-9"; journalName: "Nature"; date: 
            "2025-10"; volume: "646"; issue: "8086"; pages: "963-972"
COMMENT     Sequence Label: pEP0003_pIVTRup(BsmBI)_ABEmax
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(260..276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(260..276)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(273..295)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    284..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(308..314,315..332,333..338))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    353..374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(491..508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(662..1250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(742..761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1421..2212,2213..2281))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2044..2063
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2299..3093)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2410..2429)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3020..3039
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(3437..3865)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3547..3568)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3759..3778
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3991..4013
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4005..4022
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4006..4022
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4029..4047
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4119..4137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4219..4239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5428..5451,5452..5454,5455..9528)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9571..9591
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctggccatg cccttcttct ctcccttgca cctgtacctc ttggtctttg aataaagcct
       61 gagtaggaag tgagggtcta gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      121 aaaaaaaaaa aaaaaaaatg agacgcgatc aataaccgac actcctgcgt cgcctacgcg
      181 ctacgatctg cagaattcca gcacactggc ggccgttact agtggatccg agctcggtac
      241 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      301 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      361 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      421 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      481 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      541 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      601 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      661 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      721 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      781 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      841 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      901 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      961 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1021 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1081 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1141 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1201 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1261 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1321 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1381 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1441 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1501 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1561 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1621 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1681 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1741 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1801 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1861 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1921 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1981 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2041 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2101 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2161 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2221 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2281 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     2341 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     2401 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     2461 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2521 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2581 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2641 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2701 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2761 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2821 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2881 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2941 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     3001 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     3061 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     3121 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     3181 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     3241 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     3301 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     3361 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     3421 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3481 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3541 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3601 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3661 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3721 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3781 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3841 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3901 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3961 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4021 gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg
     4081 gccgccagtg tgatggattt ggaccctcgt acagaagcta atacgactca ctatagggaa
     4141 ataagagaga aaagaagagt aagaagaaat ataagagcca ccatgaaacg gacagccgac
     4201 ggaagcgagt tcgagtcacc aaagaagaag cggaaagtct ctgaagtcga gtttagccac
     4261 gagtattgga tgaggcacgc actgaccctg gcaaagcgag catgggatga aagagaagtc
     4321 cccgtgggcg ccgtgctggt gcacaacaat agagtgatcg gagagggatg gaacaggcca
     4381 atcggccgcc acgaccctac cgcacacgca gagatcatgg cactgaggca gggaggcctg
     4441 gtcatgcaga attaccgcct gatcgatgcc accctgtatg tgacactgga gccatgcgtg
     4501 atgtgcgcag gagcaatgat ccacagcagg atcggaagag tggtgttcgg agcacgggac
     4561 gccaagaccg gcgcagcagg ctccctgatg gatgtgctgc accaccccgg catgaaccac
     4621 cgggtggaga tcacagaggg aatcctggca gacgagtgcg ccgccctgct gagcgatttc
     4681 tttagaatgc ggagacagga gatcaaggcc cagaagaagg cacagagctc caccgactct
     4741 ggaggatcta gcggaggatc ctctggaagc gagacaccag gcacaagcga gtccgccaca
     4801 ccagagagct ccggcggctc ctccggagga tcctctgagg tggagttttc ccacgagtac
     4861 tggatgagac atgccctgac cctggccaag agggcacgcg atgagaggga ggtgcctgtg
     4921 ggagccgtgc tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc
     4981 ctgcacgacc caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg
     5041 cagaactaca gactgattga cgccaccctg tacgtgacat tcgagccttg cgtgatgtgc
     5101 gccggcgcca tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaacgcaaaa
     5161 accggcgccg caggctccct gatggacgtg ctgcactacc ccggcatgaa tcaccgcgtc
     5221 gaaattaccg agggaatcct ggcagatgaa tgtgccgccc tgctgtgcta tttctttcgg
     5281 atgcctagac aggtgttcaa tgctcagaag aaggcccaga gctccaccga ctccggagga
     5341 tctagcggag gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa
     5401 agcagcgggg gcagcagcgg ggggtcagac aagaagtaca gcatcggcct ggccatcggc
     5461 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     5521 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     5581 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     5641 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     5701 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     5761 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     5821 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     5881 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     5941 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     6001 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     6061 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     6121 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     6181 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     6241 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     6301 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     6361 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     6421 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     6481 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     6541 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     6601 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     6661 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     6721 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     6781 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     6841 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     6901 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     6961 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     7021 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     7081 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     7141 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     7201 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     7261 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     7321 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     7381 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     7441 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     7501 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     7561 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     7621 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     7681 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     7741 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     7801 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     7861 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     7921 ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc
     7981 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     8041 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     8101 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     8161 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     8221 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     8281 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     8341 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     8401 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     8461 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     8521 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     8581 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     8641 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     8701 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     8761 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     8821 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     8881 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     8941 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     9001 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     9061 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     9121 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     9181 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     9241 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     9301 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     9361 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     9421 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     9481 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgactc tggcggctca
     9541 aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctgaatcgct
     9601 gccttctgcg gggcttgcct
//
