LOCUS       sequence-493722-        9423 bp    DNA     circular SYN 24-NOV-2025
DEFINITION  Mammalian Expression of HsRoquin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoglu-Ozturk 
            D, Weichenrieder O, Izaurralde E
  TITLE     A CAF40-binding motif facilitates recruitment of the CCR4-NOT 
            complex to mRNAs targeted by Drosophila Roquin.
  JOURNAL   Nat Commun. 2017 Feb 6;8:14307. doi: 10.1038/ncomms14307.
  PUBMED    28165457
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-6"; volume: "8:14307. doi"; pages: " 
            10.1038/ncomms14307"
COMMENT     Sequence Label: pcDNA3.1-MS2-HA-HsRoquin2_AE
FEATURES             Location/Qualifiers
     source          1..9423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1333..1359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(4976..4994)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4977..4994)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5014..5031)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5020..5244
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5290..5718
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5377..5396)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5587..5608
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5727..5747)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5732..6061
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5912..6047
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5974..5993
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6128..6922
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6182..6201)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6792..6811
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7096..7217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7133..7152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7187..7206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7266..7282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7266..7282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7279..7301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7290..7306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7314..7320,7321..7338,7339..7344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7359..7380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7497..7514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7668..8256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7748..7767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8427..9218,9219..9287))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9050..9069
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9288..9392)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg cgacgtgact
      961 gtcgccccaa gcaacttcgc taacggggtc gctgaatgga tcagctctaa ctcgcgatca
     1021 caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg caaatacacc
     1081 atcaaagtcg aggtgcctaa agtggcaacc cagactgttg gtggtgaaga gcttcctgta
     1141 gccggatgga gatcttactt aaatatggaa ctaaccattc caattttcgc cacgaattcc
     1201 gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc
     1261 tcggccatcg cggccaactc cggcatctac ggaggtggag gtggatctgg tggatctcct
     1321 ccacatatgg cttacccata cgatgttcca gattacgctc ctccaaagct tggatccatg
     1381 cctgtgcagg cagctcaatg gacagaattt ctgtcctgtc caatctgcta taatgaattt
     1441 gatgagaatg tgcacaaacc catcagttta ggttgttcac acactgtttg caagacctgc
     1501 ttgaataaac ttcatcgaaa agcttgtcct tttgaccaga ctgccatcaa cacagatatt
     1561 gatgtacttc ctgtcaactt cgcacttctc cagttagttg gagcccaggt accagatcat
     1621 cagtcaatta agttaagtaa tctaggtgag aataaacact atgaggttgc aaagaaatgc
     1681 gttgaggatt tggcactcta cttaaaacca ctaagtggag gtaaaggtgt agctagcttg
     1741 aaccagagtg cactgagccg tccaatgcaa aggaaactgg tgacacttgt aaactgtcaa
     1801 ctggtggagg aagaaggtcg tgtaagagcc atgcgagcag ctcgttccct tggagaaaga
     1861 actgtaacag aactgatatt acagcaccag aaccctcagc agttgtctgc caatctatgg
     1921 gccgctgtca gggctcgagg atgccagttt ttagggccag ctatgcaaga agaggccttg
     1981 aagctggtgt tactggcatt agaagatggt tctgccctct caaggaaagt tctggtactt
     2041 tttgttgtgc agagactaga accaagattt cctcaggcat caaaaacaag tattggtcat
     2101 gttgtgcaac tactgtatcg agcttcttgt tttaaggtta ccaaaagaga tgaagactct
     2161 tccctaatgc agctgaagga ggaatttcgg agttatgaag cattacgcag agaacatgat
     2221 gcccaaattg ttcatattgc catggaagca ggactccgta tttcacctga acagtggtcc
     2281 tctcttttgt atggtgattt ggctcataaa tcacacatgc agtctatcat tgataagcta
     2341 cagtctccag agtcatttgc aaagagtgtc caggaattga caattgtttt gcaacgaaca
     2401 ggtgacccag ctaacttaaa tagactgagg cctcatttag agcttcttgc aaacatagac
     2461 cctaatccag acgctgtttc accaacttgg gagcagctgg aaaatgcaat ggtagctgtt
     2521 aaaacagtag ttcatggcct tgtggacttc atacaaaatt atagtagaaa aggccatgag
     2581 acccctcagc ctcagccaaa cagcaaatac aagactagca tgtgccgaga tttgcgacag
     2641 caagggggtt gtccacgagg aacaaattgt acatttgccc attctcagga agagcttgaa
     2701 aagtatcgat taaggaacaa aaagatcaat gccactgtaa gaacgtttcc tcttctaaat
     2761 aaagttggtg taaacaacac tgtcacaacc acagccggaa atgtcatttc tgtcatagga
     2821 agtactgaaa caacagggaa aattgttcca agtacaaacg gaatttcaaa tgcagaaaac
     2881 agtgtttccc agctaatctc acgtagtact gacagtacct taagagctct ggagaccgtg
     2941 aagaaagtgg gaaaggttgg cgctaatggt cagaatgctg ctgggccctc tgcagattct
     3001 gtaactgaaa ataaaattgg ttctccaccc aagactcctg taagtaatgt agcagctacc
     3061 tcagctgggc cctctaatgt tggaacagag ctgaattctg tgcctcaaaa atccagccca
     3121 tttctaacta gagtaccagt atatcctccg cattctgaaa acattcagta ttttcaagat
     3181 ccaaggactc agataccctt tgaagtccca cagtacccac agacaggata ctatccacca
     3241 cctccaacgg taccagctgg tgtggctccc tgtgttcctc gctttgtgag gtccaataac
     3301 gttccagagt cctccctccc acctgcttcc atgccatatg ccgatcatta cagtacattt
     3361 tcccctcgag atcgaatgaa ttcttctcct taccagcctc ctcctccgca gccgtatgga
     3421 ccagttcctc cagtaccttc tggaatgtat gctcctgtgt acgacagcag gcgcatctgg
     3481 cgcccaccta tgtaccaacg agatgacatt attagaagca attctttacc tccaatggat
     3541 gtgatgcact catctgtcta tcagacatct ttgcgggaaa gatataactc attagatgga
     3601 tattattcgg tggcttgtca gccaccaagt gagccaagga caactgtgcc tttaccaagg
     3661 gaaccttgtg gtcatttgaa gaccagttgc gaggagcaga taagaagaaa gccagatcag
     3721 tgggcacagt accacactca gaaagcacct cttgtctctt caactcttcc tgtggcaaca
     3781 cagtcaccaa caccaccttc tcctctgttc agtgtagact ttcgtgcgga tttctcagag
     3841 agtgtgagtg gtacaaaatt tgaagaagat catctttccc attattctcc ctggtcttgt
     3901 ggcaccatag gctcctgtat aaatgccatt gattcagagc ccaaagatgt cattgctaat
     3961 tcaaatgctg tgttaatgga cctggacagt ggtgatgtta agagaagagt acatttattt
     4021 gaaacccaga gaaggacaaa agaagaagat ccaataattc cctttagtga tggacccatc
     4081 atctcaaaat ggggtgcgat ttccagatct tcccgtacag gttaccatac cacagatcct
     4141 gtccaggcca ctgcttccca aggaagtgcg actaagccca tcagtgtatc agattatgtc
     4201 ccttatgtca atgctgttga ttcaaggtgg agttcatatg gcaacgaggc cacatcatca
     4261 gcacactatg ttgaaaggga cagattcatt gttactgatt tatctggtca tagaaagcat
     4321 tccagtactg gggacctttt gagccttgaa cttcagcagg ccaagagcaa ctcattactt
     4381 cttcagagag aggccaatgc tttggccatg caacagaagt ggaattccct ggatgaaggc
     4441 cgtcacctta ccttaaacct tttaagcaag gaaattgaac taagaaatgg agagttacag
     4501 agtgattata cagaagatgc aacagatact aaacctgata gggatatcga gttagagctt
     4561 tcagcacttg atactgatga acctgatgga caaagtgaac caattgaaga gatcttggac
     4621 atacagcttg gtatcagttc tcaaaatgat cagttgctaa atggaatggc agtggaaaat
     4681 gggcatccag tacagcagca ccaaaaggag ccaccaaagc agaagaaaca gagtttaggt
     4741 gaagaccatg tgattctgga ggagcaaaaa acaattctgc cggtaacttc ttgctttagc
     4801 cagccactcc cagtgtctat tagcaatgca agttgcctcc ccatcaccac atctgtcagt
     4861 gctggcaacc tcattctgaa aactcatgtt atgtctgaag ataaaaacga ctttttaaaa
     4921 cctgttgcaa atgggaagat ggttaacagc tgagcggccg cctagagggc cctattctat
     4981 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     5041 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5101 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5161 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5221 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5281 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5341 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5401 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5461 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5521 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5581 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5641 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5701 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5761 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5821 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5881 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5941 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6001 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6061 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     6121 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     6181 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6241 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6301 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6361 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6421 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6481 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6541 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6601 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6661 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6721 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6781 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6841 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6901 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     6961 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7021 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7081 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7201 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7261 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7321 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7381 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7441 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7501 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7561 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7981 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8041 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8101 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8161 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8221 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8281 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8341 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8401 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8461 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8521 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8581 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8641 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8701 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8761 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8821 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8881 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8941 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9001 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9061 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9121 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9181 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9241 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9301 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9361 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9421 gtc
//
