LOCUS       sequence-493789-       10737 bp    DNA     circular SYN 24-NOV-2025
DEFINITION  Lentiviral expression of alpha subunit of ATPGobble. Includes ampR 
            and bsdR (blasticidin). Use together with pLVX-TetOne-bsd-atpD 
            (#248066) and pLVX-TetOne-atpG (#248067) for active ATPGobble..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Ekvik AE, Kober MM, Titov DV
  TITLE     Genetically encoded tool for manipulation of ATP/ADP ratio in human 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 
            10.1101/2025.08.12.670003.
  PUBMED    40894546
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 
            10.1101/2025.08.12.670003."
COMMENT     Sequence Label: pLVX-TetOne-bsd-atpA
FEATURES             Location/Qualifiers
     source          1..10737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(254..1000)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(1019..1529)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1082..1100)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        1546..1913
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1554..1572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1590..1608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1626..1644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1662..1680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1698..1716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1734..1752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1770..1788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      1919..1928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3497..3520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3878..4012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4056..4171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(4434..4478)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4663..4896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5393..5518
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5565..6198
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6366..6500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6548..6652
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6653..6721,6722..7513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6871..6890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7684..8272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8173..8192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8426..8443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8560..8581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8596..8601,8602..8619,8620..8626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8634..8650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8639..8661
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8658..8674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8658..8674
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8802..9435
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9642..10230
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9748..9759
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10157..10177
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10244..10642)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(join(10651..10737,1..243))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10665..10737,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattcgcg cgtttacccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
      301 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
      361 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
      421 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
      481 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
      541 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
      601 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
      661 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
      721 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
      781 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
      841 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
      901 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
      961 gagcagagtt tatgactttg ctcttgtcca gtctagacat ggtaattcga tgatccccct
     1021 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
     1081 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
     1141 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
     1201 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
     1261 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
     1321 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
     1381 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
     1441 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
     1501 tgccttggaa aaggcgcaac cccaaccccg tggaattatc acctcgagtt tactccctat
     1561 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     1621 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     1681 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     1741 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt
     1801 tgcttatgta aaccagggcg cctataaaag agtgctgatt ttttgagtaa acttcaattc
     1861 cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt aaagaattgc
     1921 caccatggga caactgaata gcacagagat tagcgagctg atcaagcagc ggatcgccca
     1981 gttcaacgtg gtgtctgagg cccacaatga gggcaccatc gtgtccgtgt ctgacggcgt
     2041 gatcagaatc cacggactgg ccgactgtat gcagggcgag atgatcagcc tgcctggcaa
     2101 cagatacgct atcgccctga acctggaacg ggattctgtg ggcgctgtgg tcatgggccc
     2161 ttatgccgat cttgccgagg gcatgaaagt gaagtgcacc ggcagaatcc tggaagtgcc
     2221 tgttggcaga ggcctgctgg gcagagtggt taatactctg ggagccccta tcgacggcaa
     2281 gggccctctt gatcacgatg gctttagcgc cgtggaagca attgcccctg gcgtgatcga
     2341 gcggcagtct gtggatcagc cagtgcagac aggctacaag gccgtggaca gcatgatccc
     2401 tatcggcaga ggacagcggg aactgatcat cggcgataga cagaccggca agacagccct
     2461 ggccatcgac gccatcatca accagcgcga tagcggcatc aagtgcatct acgtggccat
     2521 cggccagaag gccagcacca tctctaatgt cgtgcggaag ctggaagaac acggcgccct
     2581 ggctaatacc attgtggtgg tggccacagc cagcgagtct gccgctctgc aatatctggc
     2641 cccttacgcc ggatgtgcca tgggcgagta cttcagagac agaggcgagg acgccctgat
     2701 tatctacgac gacctgagca aacaggccgt ggcctacaga cagatctccc tgctgcttag
     2761 acggcctcct ggcagagaag cttttcccgg cgacgtgttc tacctgcaca gcagactgct
     2821 ggaaagggcc gccagagtga atgccgagta cgtggaagcc ttcaccaagg gcgaagtgaa
     2881 gggcaagacc ggctctctga cagctctgcc catcatcgaa acacaggccg gggatgtgtc
     2941 cgccttcgtg cctacaaacg tgatcagcat taccgacggc cagatcttcc tggaaacaaa
     3001 cctgttcaac gccggcatca gacccgccgt gaatcctggc atctccgtgt ctagagttgg
     3061 cggcgctgcc cagaccaaga tcatgaagaa actgagcggc ggcatccgga cagcactggc
     3121 ccagtataga gaactggccg ccttcagcca gttcgccagc gatctggatg acgccaccag
     3181 aaagcagctg gaccacggcc agaaagtgac cgagctgctg aagcagaaac agtacgcccc
     3241 tatgagcgtg gcccagcaaa gcctggttct gtttgccgcc gagagaggct atctggccga
     3301 cgtggaactg agcaagattg gcagctttga ggctgccctg ctggcctatg tggacaggga
     3361 tcacgctccc ctgatgcaag agatcaatca gaccggcggc tacaacgacg agatcgaggg
     3421 aaagctgaag ggcatcctgg atagcttcaa ggccacacag tcttggggcg gctctggtgg
     3481 atctggcggc agcatggact acaaggacga cgacgacaaa tgacagacca cctcccctgc
     3541 gagctaagct ggacagccaa tgacgggtaa gagagtgaca tttttcacta acctaagaca
     3601 ggagggccgt cagagctact gcctaatcca aagacgggta aaagtgataa aaatgtatca
     3661 ctccaaccta agacaggcgc agcttccgag ggatttgaga tccagaccac ctcccctgcg
     3721 agctaagctg gacagccaat gacgggtaag agagtgacat ttttcactaa cctaagacag
     3781 gagggccgtc agagctactg cctaatccaa agacgggtaa aagtgataaa aatgtatcac
     3841 tccaacctaa gacaggcgca gcttccgagg gatttgagat ccagacatga taagatacat
     3901 tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta tttgtgaaat
     3961 ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag ttaagtcgat
     4021 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
     4081 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
     4141 tgtacccccc aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat
     4201 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     4261 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     4321 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     4381 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     4441 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     4501 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     4561 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     4621 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     4681 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     4741 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     4801 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     4861 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     4921 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     4981 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     5041 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     5101 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     5161 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     5221 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     5281 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     5341 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     5401 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     5461 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     5521 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     5581 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     5641 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     5701 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag
     5761 cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc
     5821 tccctggaaa gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga
     5881 agtactccgg atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc
     5941 actctaacac ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc
     6001 tggtgttctc tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt
     6061 agcaccatcc aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat
     6121 cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg
     6181 agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt
     6241 taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg
     6301 gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc
     6361 cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     6421 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     6481 taagctgcaa taaacaagtt gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     6541 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6601 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6661 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6721 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6781 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6841 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6901 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6961 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7021 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7081 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7141 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7201 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7261 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7321 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7381 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7441 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7501 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7561 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7621 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7801 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8281 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8341 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8401 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8461 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8521 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8581 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8641 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     8701 ggtcgacggt aaacgctagc aatgtcaagg cctctcactc tctgatattc atttctttgc
     8761 aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac
     8821 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     8881 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     8941 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
     9001 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
     9061 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
     9121 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
     9181 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
     9241 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
     9301 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
     9361 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt cgttgggagt
     9421 gaattagccc ttccagtccc ctcttttctt ttaaaaagtg gctaagatct acagctgcct
     9481 tgtaagtcat tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct
     9541 cttgtgcttc tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg
     9601 ctccatgttt ttctaggtct cgactgcaga attaattcca ggcggggagg cggcccaaag
     9661 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     9721 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     9781 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
     9841 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     9901 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     9961 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
    10021 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
    10081 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
    10141 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
    10201 ttcacaaatt ttgtaatcca gaggttgatt gttccagacg cgtttagccc tcccacacat
    10261 aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca
    10321 ctatcgcttt gatcccagga tgcagatcga gaagcacctg tcgacaccgt ccgcaggggc
    10381 tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg
    10441 cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata
    10501 cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt
    10561 cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt
    10621 cttgagacaa aggcttggcc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc
    10681 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaat
//
