LOCUS       sequence-493799-       10078 bp    DNA     circular SYN 24-NOV-2025
DEFINITION  Lentiviral expression of gamma subunit of ATPGobble. Includes ampR 
            and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA 
            (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Ekvik AE, Kober MM, Titov DV
  TITLE     Genetically encoded tool for manipulation of ATP/ADP ratio in human 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 
            10.1101/2025.08.12.670003.
  PUBMED    40894546
REFERENCE   2  (bases 1 to 10078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 
            10.1101/2025.08.12.670003."
COMMENT     Sequence Label: pLVX-TetOne-puro-atpG
FEATURES             Location/Qualifiers
     source          1..10078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             91..724
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(852..868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(852..868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(865..887)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    876..892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(900..906,907..924,925..930))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    945..966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1083..1100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1254..1842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1334..1353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2013..2804,2805..2873))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2636..2655
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2874..2978)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3026..3160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             3328..3961
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4008..4133
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4630..4863
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5048..5092
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5355..5470
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    5514..5648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5827..5850)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6738..6747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6753..7120)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6878..6896
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6914..6932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6950..6968
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6986..7004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7022..7040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7058..7076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7094..7112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7137..7647
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7566..7584
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7666..8412
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        8423..8752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8603..8738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8761..9360
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8761..8780)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9257..9277
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9374..9962
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9427..9447)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9845..9856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
       61 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
      121 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
      181 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
      241 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
      301 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
      361 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
      421 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
      481 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
      541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      721 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
      781 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
      841 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      901 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      961 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1021 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1081 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1141 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3001 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     3061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3121 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     3181 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     3241 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     3301 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     3361 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     3421 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     3481 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     3541 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     3601 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     3661 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     3721 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     3781 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3841 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3901 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3961 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     4021 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     4081 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     4141 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4201 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     4261 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     4321 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     4381 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     4441 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     4501 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     4561 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     4621 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     4681 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     4741 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     4801 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     4861 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     4921 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     4981 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     5041 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     5101 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     5161 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     5221 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     5281 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     5341 tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5401 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5461 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcga cttaacttgt
     5521 ttattgcagc ttataatggt tacaaataag gcaatagcat cacaaatttc acaaataagg
     5581 catttttttc actgcattct agttttggtt tgtccaaact catcaatgta tcttatcatg
     5641 tctggatctc aaatccctcg gaagctgcgc ctgtcttagg ttggagtgat acatttttat
     5701 cacttttacc cgtctttgga ttaggcagta gctctgacgg ccctcctgtc ttaggttagt
     5761 gaaaaatgtc actctcttac ccgtcattgg ctgtccagct tagctcgcag gggaggtggt
     5821 ctgtcactta tcatcatcgt ctttgtaatc catggatcct ccgcttccgc cacttccacc
     5881 aacagcggca gcgccagaca cgatctcggt cagttcttgg gtgatactgg cctgccgggc
     5941 cttgttgtac accagctgca gttctttgat caggctgccg ccgttatcgg tggcagcctt
     6001 catagccacc attctagcgg cctgttcgct ggccaggttt tccacaacgc cctggtacac
     6061 ctggctttcc acgtatctcc gcagcagggt atccagcagt gccttaggat cgggctcgta
     6121 caggtagtcc cagctcttgt gcttcaggtc atcgtcgtcg ctggcaggca gaggcaacag
     6181 ctggctgatg gttggcacct ggctcatggt gttgatgaac ttgttagaca cgatgtacag
     6241 cttatccagc cggccttcat cgtaggcctg cagcatgact ttcacggggc cgatcagctc
     6301 agacagagat ggattgtcgc ccatgcctgt cacttgggcc accacgtttc cgccaacgct
     6361 gttgaagaag ctgacgccct tagagccgat catggccagg tcgcactgca cccctttgtc
     6421 ggtccaggtc ttcatctcgg ccagcagttt cttgaacaga ttgatgttca ggccgccaca
     6481 caggcctcta tctgtagaca ccaccaggta gcccacgcgc ttcacgtccc gatcttccag
     6541 gtaggggtgc ttgtactcga ggttgccgtg ggccagatgg ccaatcactt tccgcattgt
     6601 ctcggcgtat ggtctggagg cggccatcct atcctgggac tttctcatct tgctggcggc
     6661 caccatttcc atggccttgg tgattttctg ggtgttctgc acgctggcga tcttggatct
     6721 gatctctttg gcgcctgcca tggtggcaat tctttacgag ggtaggaagt ggtacggaaa
     6781 gttggtataa gacaaaagtg ttgtggaatt gaagtttact caaaaaatca gcactctttt
     6841 ataggcgccc tggtttacat aagcaaagct tatacgttct ctatcactga tagggagtaa
     6901 actggatata cgttctctat cactgatagg gagtaaactg tagatacgtt ctctatcact
     6961 gatagggagt aaactggtca tacgttctct atcactgata gggagtaaac tccttatacg
     7021 ttctctatca ctgataggga gtaaagtctg catacgttct ctatcactga tagggagtaa
     7081 actcttcata cgttctctat cactgatagg gagtaaactc gaggtgataa ttccacgggg
     7141 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     7201 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     7261 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     7321 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     7381 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     7441 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     7501 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     7561 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     7621 gaccgaatca ccgacctctc tccccagggg gatcatcgaa ttaccatgtc tagactggac
     7681 aagagcaaag tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg
     7741 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     7801 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     7861 cactcctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     7921 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     7981 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     8041 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     8101 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     8161 atgcccccac ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     8221 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     8281 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     8341 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     8401 ctccccgggt aaacgcgcga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaacgcgacc
     8761 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     8821 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     8881 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     8941 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     9001 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     9061 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     9121 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     9181 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     9241 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     9301 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     9361 acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9421 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9481 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9541 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9601 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9661 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9721 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     9781 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9841 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9901 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9961 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
    10021 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgg
//
