LOCUS       sequence-493802-       10554 bp    DNA     circular SYN 24-NOV-2025
DEFINITION  Lentiviral expression of a catalytically inactive mutant of the beta
            subunit of ATPGobble. Use together with pLVX-TetOne-bsd-atpA 
            (#248065) and pLVX-TetOne-puro-atpG (#248067) for a LOF control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ekvik AE, Kober MM, Titov DV
  TITLE     Genetically encoded tool for manipulation of ATP/ADP ratio in human 
            cells.
  JOURNAL   bioRxiv [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 
            10.1101/2025.08.12.670003.
  PUBMED    40894546
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 24, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Aug 23:2025.08.12.670003. doi: 
            10.1101/2025.08.12.670003."
COMMENT     Sequence Label: pLVX-TetOne-bleo-atpD-K155Q
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(254..1000)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(1019..1529)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1082..1100)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        1546..1913
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1554..1572
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1590..1608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1626..1644
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1662..1680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1698..1716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1734..1752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1770..1788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      1919..1928
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3338..3361
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3719..3853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3897..4012
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(4275..4319)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4504..4737
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5234..5359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5406..6039
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6207..6341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6389..6493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6494..6562,6563..7354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6712..6731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7525..8113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8014..8033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8267..8284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8401..8422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8437..8442,8443..8460,8461..8467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8475..8491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8480..8502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8499..8515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8499..8515
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             8643..9276
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9483..10071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9589..9600
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9998..10018
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10085..10459)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(join(10468..10554,1..243))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10482..10554,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattcgcg cgtttacccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
      301 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
      361 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
      421 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
      481 ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
      541 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
      601 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctgatttt
      661 ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta gccccgtcgc
      721 gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag tcttgccatg
      781 actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg attggcaggg
      841 catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc tcaactccca
      901 gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg agtaattcca
      961 gagcagagtt tatgactttg ctcttgtcca gtctagacat ggtaattcga tgatccccct
     1021 ggggagagag gtcggtgatt cggtcaacga gggagccgac tgccgacgtg cgctccggag
     1081 gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc ccacactacc gccccacacc
     1141 ccgcctcccg caccgcccct tcccggccgc tgctctcggc gcgccctgct gagcagccgc
     1201 tattggccac agcccatcgc ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg
     1261 cgagggtact agtgagacgt gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca
     1321 aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc cggggggccc acaagggtag
     1381 cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg
     1441 cgccgctgcg tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc
     1501 tgccttggaa aaggcgcaac cccaaccccg tggaattatc acctcgagtt tactccctat
     1561 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     1621 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     1681 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     1741 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt
     1801 tgcttatgta aaccagggcg cctataaaag agtgctgatt ttttgagtaa acttcaattc
     1861 cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt aaagaattgc
     1921 caccatggga gccaccggaa agatcgtgca agtgatcgga gccgtggtgg acgtggaatt
     1981 ccctcaggat gctgtgccca gagtgtacga cgccctcgag gtgcagaacg gcaatgagag
     2041 actggtgctc gaagtgcagc agcagcttgg cggcggaatc gtcagaacaa tcgccatggg
     2101 cagcagcgac ggcctgagaa gaggactgga tgtgaaggac ctggaacacc ccatcgaggt
     2161 gccagtggga aaagccacac tgggcagaat catgaacgtg ctgggcgagc ccgtggacat
     2221 gaagggcgaa attggcgaag aggaaagatg ggccatccac agggccgctc ctagctacga
     2281 ggaactgtcc aacagccaag agctgctcga gacaggcatc aaagtgatcg acctgatgtg
     2341 ccccttcgcc aaaggcggca aagtgggact ttttggcgga gccggcgtgg gacagaccgt
     2401 gaacatgatg gaactgattc ggaatatcgc catcgagcac agcggctact ctgtgtttgc
     2461 tggcgtgggc gagagaacca gagagggcaa cgatttctac cacgagatga ccgacagcaa
     2521 cgtgatcgac aaggtgtccc tggtgtatgg ccagatgaac gagccaccag gcaacagact
     2581 gagagtggcc ctgacaggac tgaccatggc cgagaagttt cgcgacgagg gcagagatgt
     2641 gctgctgttc gtggacaaca tctaccggta caccctggcc ggcacagaag tgtctgctct
     2701 gctgggaaga atgccttccg ccgtgggcta tcagcctaca ctggccgaag aaatgggcgt
     2761 gctgcaagag agaatcacca gcaccaagac aggcagcatc acctctgtgc aggccgtgta
     2821 tgtgcctgcc gacgatctga ccgatccttc tcctgccacc acattcgccc acctggatgc
     2881 tacagtggtg ctgtctaggc agattgccag cctgggcatc tatcctgctg tggcccctct
     2941 ggatagcacc tccagacaac tggatcctct ggtcgtgggc caagagcact acgatacagc
     3001 tagaggcgtg cagagcatcc tgcagcggta tcaagagctg aaggatatca ttgccatcct
     3061 cggcatggac gagctgtccg aagaggacaa gctggtggtc gccagggcca gaaaaatcca
     3121 gcggtttctg agccagccat tcttcgtggc cgaggtgttc acaggcagcc ccggcaaata
     3181 tgtgtccctg aaggacacca tccggggctt caagggaatc atggaaggcg agtacgacca
     3241 tctgcctgaa caggccttct acatggtcgg atccatcgag gaagccgtgg aaaaggccaa
     3301 gaagctcgga ggcagcggag gttctggcgg ctctatggat tataaggacg acgacgacaa
     3361 atgacagacc acctcccctg cgagctaagc tggacagcca atgacgggta agagagtgac
     3421 atttttcact aacctaagac aggagggccg tcagagctac tgcctaatcc aaagacgggt
     3481 aaaagtgata aaaatgtatc actccaacct aagacaggcg cagcttccga gggatttgag
     3541 atccagacca cctcccctgc gagctaagct ggacagccaa tgacgggtaa gagagtgaca
     3601 tttttcacta acctaagaca ggagggccgt cagagctact gcctaatcca aagacgggta
     3661 aaagtgataa aaatgtatca ctccaaccta agacaggcgc agcttccgag ggatttgaga
     3721 tccagacatg ataagataca ttgatgagtt tggacaaacc aaaactagaa tgcagtgaaa
     3781 aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta tttgtaacca ttataagctg
     3841 caataaacaa gttaagtcga taaactggat ctctgctgtc cctgtaataa acccgaaaat
     3901 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     3961 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaggcgatac
     4021 cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
     4081 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
     4141 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     4201 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     4261 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     4321 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     4381 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     4441 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     4501 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     4561 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     4621 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     4681 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     4741 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     4801 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
     4861 tctcctcctc caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt
     4921 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
     4981 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
     5041 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
     5101 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
     5161 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
     5221 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
     5281 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
     5341 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
     5401 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     5461 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     5521 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     5581 agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc
     5641 cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag
     5701 ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg
     5761 ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca
     5821 ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg
     5881 gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc
     5941 ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa
     6001 ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat
     6061 atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt
     6121 aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga
     6181 ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg
     6241 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     6301 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tgatctcccg atccgtcgac
     6361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6661 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6721 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6781 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6841 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6901 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6961 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7021 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7081 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7141 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7201 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7261 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7321 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7381 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7441 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8161 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8221 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8281 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     8341 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     8401 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8461 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     8521 ttacgccaag ctctagctag aggtcgacgg taaacgctag caatgtcaag gcctctcact
     8581 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
     8641 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     8701 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     8761 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     8821 cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag
     8881 tcccgcccag gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc
     8941 gatatcagca gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta
     9001 ggcggctgtc aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc
     9061 tcacagggtg taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct
     9121 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg
     9181 gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
     9241 tatcttgtct tcgttgggag tgaattagcc cttccagtcc cctcttttct tttaaaaagt
     9301 ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg
     9361 gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg
     9421 ctgtattgct acttgtgatt gctccatgtt tttctaggtc tcgactgcag aattaattcc
     9481 aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg
     9541 aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga
     9601 agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca
     9661 tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc
     9721 ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg
     9781 gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt
     9841 tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc
     9901 ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc
     9961 aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata
    10021 gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgttccagac
    10081 gcgttcagtc ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg
    10141 cgaactcccg cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc
    10201 ggaagttcgt ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc
    10261 acacccaggc cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg
    10321 tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga
    10381 gccggtcggt ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg
    10441 cactggtcaa cttggccatg gtcgcgtttt gcaaaagcct aggcctccaa aaaagcctcc
    10501 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaat
//
