LOCUS       sequence-493992-        4296 bp    DNA     circular SYN 26-NOV-2025
DEFINITION  express cCadC AtRbcL fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T
  TITLE     Directed evolution of a plant Rubisco chaperone with altered client 
            recognition.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 
            10.1073/pnas.2510701122. Epub 2025 Sep 11.
  PUBMED    40932770
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 26, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-09-16"; volume: "122(37):e2510701122. 
            doi"; pages: " 10.1073/pnas.2510701122. Epub 2025 Sep 11"
COMMENT     Sequence Label: pBL155
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(252..271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     412..434
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     protein_bind    816..834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        835..889
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    840..858
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    870..888
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2888..2919
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2935..3594)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3509..3528
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3595..3697)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      3708..4296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4197..4216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
      661 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacaagagg
      721 acatccggtc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttgctgag
      781 gagacttagg gaccctccat cgaatggcca gatgatccct atcagtgata gagattgaca
      841 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga
      901 atagttcgac aaaaatctag acctgcaggt gcagtaaagg aaaaaaaaaa tgcaacaacc
      961 tgtagttcgc gttggcgaat ggcttgttac tccgtccata aaccaaatta gccgcaatgg
     1021 gcgtcaactt acccttcgtc cgagattaat cgatcttctg gttttctttg ctcaacacag
     1081 tggcgaagta cttagcaggg atgaacttat cgataatgtc tggaagagaa gtattgtcac
     1141 caatcacgtt gtgacgcaga gtatctcaga actacgtaag tcattaaaag ataatgatga
     1201 agatagtcct gtctatatcg ctactgtacc aaagcgcggc tataaattaa tggtgccggt
     1261 tatctggtac agcgaagaag agggagagga aataatgcta tcttcggcag cggctatctc
     1321 tgaggcggtt cctgccacag attctccctc ccacagtctt aacattcaaa acaccgcaac
     1381 gccacctgaa caatccccag ttaaaagcaa acgaggtgga tctggtggtg caccacaaac
     1441 agagactaaa gcaagtgttg ggttcaaagc tggtgttaaa gagtataaat tgacttacta
     1501 tactcctgaa tatgaaacca aggatactga tatcttggca gcattccgag taactcctca
     1561 acctggagtt ccacctgaag aagcaggggc tgcggtagct gctgaatctt ctactggtac
     1621 atggacaact gtgtggaccg atgggcttac cagccttgat cgttacaaag gacgatgcta
     1681 ccacatcgag cccgttccag gagaagaaac tcaatttatt gcgtatgtag cttatccctt
     1741 agaccttttt gaagaaggtt cggttactaa catgtttacc tcgattgtgg gtaatgtatt
     1801 tgggttcaaa gccctggctg ctctacgtct agaggatctg cgaatccctc ctgcttatac
     1861 taaaactttc caaggaccac ctcatggtat ccaagttgaa agagataaat tgaacaagta
     1921 tggacgtccc ctattaggat gtactattaa accaaaattg gggttatccg cgaaaaacta
     1981 tggtagagca gtttatgaat gtctacgtgg tggacttgat tttaccaaag atgatgagaa
     2041 tgtgaactcc caaccattta tgcgttggag agaccgtttc ttattttgtg ccgaagctat
     2101 ttataaatca caggctgaaa caggtgaaat caaagggcat tatttgaatg ctactgcggg
     2161 tacatgcgaa gaaatgatca aaagagctgt atttgccaga gaattgggag ttcctatcgt
     2221 aatgcatgac tacttaacag ggggattcac cgcaaatact agtttgtctc attattgccg
     2281 agataatggc ctacttcttc acatccaccg tgcaatgcac gctgttattg atagacagaa
     2341 gaatcatggt atgcacttcc gtgtactagc taaagcttta cgtctatctg gtggagatca
     2401 tattcacgcg ggtacagtag taggtaaact tgaaggagac agggagtcaa ctttgggctt
     2461 tgttgattta ctgcgcgatg attatgttga aaaagatcga agccgcggta tctttttcac
     2521 tcaagattgg gtctcactac ctggtgttct gcctgtggct tcagggggta ttcacgtttg
     2581 gcacatgcct gctttgaccg agatctttgg agatgattct gtactacaat tcggtggagg
     2641 aactttaggc cacccttggg gaaatgcacc gggtgccgta gccaaccgag tagctctgga
     2701 agcatgtgta caagctcgta atgagggacg tgatcttgca gtcgagggta atgaaattat
     2761 ccgtgaagct tgcaaatgga gtcctgaact agctgctgct tgtgaagtat ggaaagagat
     2821 cacatttaac ttcccaacca tcgataaatt agatggccaa gagtaaatta acggcactcc
     2881 tcagccaagt caaaagcctc cgaccggagg cttttgacta catgcccatg gcgtttacgc
     2941 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     3001 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     3061 cgtataatat ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     3121 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     3181 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     3241 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3301 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     3361 tttcattgcc atacggaact ccggatgagc attcatcagg cgagcaagaa tgtgaataaa
     3421 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     3481 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     3541 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     3601 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     3661 attatggtga aagttggaac ctcttacgtg ccattcaaaa taggccgttg agatcctttt
     3721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3781 ttgccggatc aagagctacc acctcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4261 ttgtgatgct cgtcaggggg gcggagccta tggaaa
//
