LOCUS       sequence-494098-        8635 bp    DNA     circular SYN 01-DEC-2025
DEFINITION  Express DCAF5(1-601) in Insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Radko-Juettner S, Yue H, Myers JA, Carter RD, Robertson AN, Mittal 
            P, Zhu Z, Hansen BS, Donovan KA, Hunkeler M, Rosikiewicz W, Wu Z, 
            McReynolds MG, Roy Burman SS, Schmoker AM, Mageed N, Brown SA, 
            Mobley RJ, Partridge JF, Stewart EA, Pruett-Miller SM, Nabet B, Peng
            J, Gray NS, Fischer ES, Roberts CWM
  TITLE     Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing 
            SWI/SNF.
  JOURNAL   Nature. 2024 Apr;628(8007):442-449. doi: 10.1038/s41586-024-07250-1.
            Epub 2024 Mar 27.
  PUBMED    38538798
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07250-1"; journalName: "Nature"; date: 
            "2024-04"; volume: "628"; issue: "8007"; pages: "442-449"
COMMENT     Sequence Label: pHoY109_pAC8_hsDCAF5_1-601
FEATURES             Location/Qualifiers
     source          1..8635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      57..645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     546..565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     799..816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     1208..2035
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1208..1332
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1370..1975)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(2039..2072)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2262..2310
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2335..2354
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2388..3065)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(2648..2667)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(3074..3183)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        3238..3329
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3254..3271
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3347..3370
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3380..3424
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             3443..3463
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_recomb     5315..6672
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5331..6672)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      6819..7274
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6906..6925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7116..7137
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7291..7310)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7410..7432
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7470..7488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7556..7660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7661..7729,7730..8521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7879..7898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
       61 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      781 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      841 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gaacggctcc gcccactatt
      901 aatgaaatta aaaattccaa ttttaaaaaa cgcagcaaga gaaacatttg tatgaaagaa
      961 tgcgtagaag gaaagaaaaa tgtcgtcgac atgctgaaca acaagattaa tatgcctccg
     1021 tgtataaaaa aaatattgaa cgatttgaaa gaaaacaatg taccgcgcgg cggtatgtac
     1081 aggaagaggt ttatactaaa ctgttacatt gcaaacgtgg tttcgtgtgc caagtgtgaa
     1141 aaccgatgtt taatcaaggc tctgacgcat ttctacaacc acgactccaa gtgtgtgggt
     1201 gaagtcatgc atcttttaat caaatcccaa gatgtgtata aaccaccaaa ctgccaaaaa
     1261 atgaaaactg tcgacaagct ctgtccgttt gctggcaact gcaagggtct caatcctatt
     1321 tgtaattatt gaataataaa acaattataa atgtcaaatt tgttttttat taacgataca
     1381 aaccaaacgc aacaagaaca tttgtagtat tatctataat tgaaaacgcg tagttataat
     1441 cgctgaggta atatttaaaa tcattttcaa atgattcaca gttaatttgc gacaatataa
     1501 ttttattttc acataaacta gacgccttgt cgtcttcttc ttcgtattcc ttctcttttt
     1561 catttttctc ctcataaaaa ttaacatagt tattatcgta tccatatatg tatctatcgt
     1621 atagagtaaa ttttttgttg tcataaatat atatgtcttt tttaatgggg tgtatagtac
     1681 cgctgcgcat agtttttctg taatttacaa cagtgctatt ttctggtagt tcttcggagt
     1741 gtgttgcttt aattattaaa tttatataat caatgaattt gggatcgtcg gttttgtaca
     1801 atatgttgcc ggcatagtac gcagcttctt ctagttcaat tacaccattt tttagcagca
     1861 ccggattaac ataactttcc aaaatgttgt acgaaccgtt aaacaaaaac agttcacctc
     1921 ccttttctat actattgtct gcgagcagtt gtttgttgtt aaaaataaca gccattgtaa
     1981 tgagacgcac aaactaatat cacaaactgg aaatgtctat caatatatag ttgctgatat
     2041 aacttcgtat agcatacatt atacgaagtt atctggttga aacgtacccg tagtggctat
     2101 ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     2161 ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
     2221 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     2281 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     2341 ccttccgtgt ttcagttagc ctcccccatc tcccgctatt actcgagtca ctgggagccg
     2401 gagtggcggg cctcggcgtg ctcgtactgc tccaccacgg tgtagtcctc gttgtggttg
     2461 gtgatgtcca gcttggagtc cacgtagtgg ttgccgggca gctgcacggg cttcttggcc
     2521 ttgtacacgg tcttgaagtc gcaggtgtag tggccgccgt ccttcagctt cagggcgtgg
     2581 gagatctcgc ccttcagcac gccgtcctgg gggtacagct tctcggtgga gggctcccag
     2641 ccggcagtct tcttctgcat tacggggccg tcggcgggga agttcacgcc cttgaacttc
     2701 accttgtaga tgaaggtgcc gtcctgcagg gaggagtcct gctgcacctc caccacgccg
     2761 ccgtcctcga agttcatgga gcgctcccag gtgaagccct cggggaagga cagcttcatg
     2821 tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtactg gaactggggg
     2881 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcacctgcag cttggcggtc
     2941 tgggtgccct cgtagggctt gccctcgccc tcgccctcga tctcgaagta gtggccgttc
     3001 acggagccct ccatgcgcac cttgaactgc atgaactcct tgatgacgtc ctcggtgttg
     3061 tccatggtag agtgattgta aataaaatgt aatttacagt atagtatttt aattaatata
     3121 caaatgattt gataataatt cttatttaac tataatatat tgtgttgggt tgaattaaag
     3181 gtccgtatac tagtatcgat tcgcgaccta ctccggaata ttaatagagt tgctgatatc
     3241 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
     3301 acagttttgt aataaaaaaa cctataaata cggatctacc atggactgga gccacccgca
     3361 gttcgaaaaa tccgcggtcg gcctgaacga catcttcgag gctcagaaaa tcgaatggca
     3421 cgaaggtgga ggaggttctg gagagaacct ctacttccag ggaggcggcc gcatgaagag
     3481 gagggcggga ctcggtggat caatgcgctc cgtcgtcgga ttcttgtccc aacgtggtct
     3541 tcatggtgac ccccttttga cgcaagactt tcaacgcagg agacttcgcg gatgtcgcaa
     3601 cctttataag aaggacctcc tcggccactt tggctgtgtc aacgctatcg agttctccaa
     3661 taatggtggt cagtggctcg tgtcaggtgg agatgacagg cgcgtactgc tgtggcacat
     3721 ggaacaagcc atacacagta gggttaaacc gatccaactc aagggcgagc atcattccaa
     3781 tatcttttgc ctcgcgttca actccggtaa cacgaaagta ttttcaggtg gaaacgatga
     3841 gcaagtaatt ctgcatgacg ttgaatcctc tgaaaccttg gacgtgttcg cccatgagga
     3901 tgcagtctac ggactgtctg ttagcccagt caacgacaac attttcgctt cttcttcaga
     3961 tgatggaaga gtccttatat gggatattag agaatcacca cacggtgagc cattttgctt
     4021 ggcaaactat cctagtgctt tccactccgt gatgttcaac ccggtcgagc ctcgtcttct
     4081 cgcgaccgct aactccaagg agggagtcgg cttgtgggat atcagaaagc cacagtccag
     4141 tttgctgaga tacggaggaa atttgtctct ccagtccgcg atgtcagttc gttttaattc
     4201 caacggtacg caattgctgg ccctgcgcag gagactgccg cctgtattgt atgatattca
     4261 cagtcgcctt ccggtcttcc agttcgacaa tcagggttac tttaactcct gcaccatgaa
     4321 atcctgctgt ttcgctggcg acagggatca gtatatcctc tccggctcag acgactttaa
     4381 cttgtacatg tggaggatcc cggctgatcc agaggcaggc ggcatcggcc gcgttgttaa
     4441 tggtgcattc atggtactta aaggacacag gagtatcgtg aatcaggtca ggtttaatcc
     4501 tcacacctat atgatttgtt catcaggtgt agagaaaatt atcaagatct ggtcacccta
     4561 taagcaacca ggttgtacag gcgatctcga cggacgtata gaggacgatt caagatgtct
     4621 ttacacccat gaagagtaca tttctttggt cctgaattcc ggttcaggcc tctcacatga
     4681 ttatgcgaac cagtctgtgc aagaggatcc ccgtatgatg gccttcttcg actccctcgt
     4741 gcgcagagaa atcgagggtt ggtcaagtga tagcgactcc gatctctccg agagcactat
     4801 tctccaactg cacgcgggcg tctctgagag gtccggttac actgattccg agtcttccgc
     4861 gagcctcccg agatctcctc cacctacggt cgacgagagt gcggataacg cttttcacct
     4921 cggaccgctt agagtgacta caacgaatac cgtggcatcc actccgccta cgccgacctg
     4981 tgaggacgct gcctctaggc agcaacgtct ttccgctttg cgcagatatc aggataaacg
     5041 cttgttggct cttagtaatg aaagcgatag tgaagaaaat gtatgtgagg tagaactgga
     5101 tacggacctc tttccacgtc cgcgttcccc ctcacccgaa gacgaatcta gttccagctc
     5161 ctccagttca tcaagtgaag acgaggaaga attgaatgag agacgcgcct ccacttggca
     5221 aagaaacgct atgagacgcc gccagaaaac taccagggag gataagccga gcgcgtgagg
     5281 tacccccgga cggccgctta attaattgat ccgggttatt agtacattta ttaagcgcta
     5341 gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat
     5401 ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat
     5461 ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac
     5521 aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct caacgccaca
     5581 aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg tgttttgttt
     5641 tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct ttcatcactg
     5701 tcgttagtgt acaattgact cgacgtaaac acgttaaata aagcttggac atatttaaca
     5761 tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa
     5821 gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt gtcggctaac
     5881 acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg cgggcgtttt
     5941 tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt agaaagcgat
     6001 ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg tggtggagct
     6061 gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg cggaggcgga
     6121 ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg caacacagtc
     6181 ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg tctgagacga
     6241 gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca
     6301 gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat cgatggtggt
     6361 ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg cggtgccgcc
     6421 ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc
     6481 gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg tggcaattca
     6541 atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg
     6601 tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta aagagattgt
     6661 ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta ctacagcatt
     6721 gtagtggcga gacacttcgc tgtcgtcgcc tgatgcggta ttttctcctt acgcatctgt
     6781 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     6841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6901 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6961 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7021 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7081 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7141 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     7201 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7261 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7321 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7381 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7441 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7681 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7741 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7801 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7861 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7921 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7981 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8041 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8101 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8161 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8221 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8281 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8341 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8401 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8461 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaa
//
