LOCUS       sequence-494099-        9658 bp    DNA     circular SYN 01-DEC-2025
DEFINITION  Express DCAF5(full length) in Insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Radko-Juettner S, Yue H, Myers JA, Carter RD, Robertson AN, Mittal 
            P, Zhu Z, Hansen BS, Donovan KA, Hunkeler M, Rosikiewicz W, Wu Z, 
            McReynolds MG, Roy Burman SS, Schmoker AM, Mageed N, Brown SA, 
            Mobley RJ, Partridge JF, Stewart EA, Pruett-Miller SM, Nabet B, Peng
            J, Gray NS, Fischer ES, Roberts CWM
  TITLE     Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing 
            SWI/SNF.
  JOURNAL   Nature. 2024 Apr;628(8007):442-449. doi: 10.1038/s41586-024-07250-1.
            Epub 2024 Mar 27.
  PUBMED    38538798
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07250-1"; journalName: "Nature"; date: 
            "2024-04"; volume: "628"; issue: "8007"; pages: "442-449"
COMMENT     Sequence Label: pHoY110_pAC8_hsDCAF5_FL
FEATURES             Location/Qualifiers
     source          1..9658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      57..645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     546..565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     799..816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     1208..2035
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1208..1332
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1370..1975)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(2039..2072)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2262..2310
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2335..2354
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2388..3065)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(2648..2667)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(3074..3183)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        3238..3329
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3254..3271
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3347..3370
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3380..3424
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             3443..3463
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_recomb     6338..7695
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6354..7695)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      7842..8297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7929..7948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8139..8160
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8314..8333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8433..8455
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8493..8511)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8579..8683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8684..8752,8753..9544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8902..8921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
       61 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      781 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      841 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gaacggctcc gcccactatt
      901 aatgaaatta aaaattccaa ttttaaaaaa cgcagcaaga gaaacatttg tatgaaagaa
      961 tgcgtagaag gaaagaaaaa tgtcgtcgac atgctgaaca acaagattaa tatgcctccg
     1021 tgtataaaaa aaatattgaa cgatttgaaa gaaaacaatg taccgcgcgg cggtatgtac
     1081 aggaagaggt ttatactaaa ctgttacatt gcaaacgtgg tttcgtgtgc caagtgtgaa
     1141 aaccgatgtt taatcaaggc tctgacgcat ttctacaacc acgactccaa gtgtgtgggt
     1201 gaagtcatgc atcttttaat caaatcccaa gatgtgtata aaccaccaaa ctgccaaaaa
     1261 atgaaaactg tcgacaagct ctgtccgttt gctggcaact gcaagggtct caatcctatt
     1321 tgtaattatt gaataataaa acaattataa atgtcaaatt tgttttttat taacgataca
     1381 aaccaaacgc aacaagaaca tttgtagtat tatctataat tgaaaacgcg tagttataat
     1441 cgctgaggta atatttaaaa tcattttcaa atgattcaca gttaatttgc gacaatataa
     1501 ttttattttc acataaacta gacgccttgt cgtcttcttc ttcgtattcc ttctcttttt
     1561 catttttctc ctcataaaaa ttaacatagt tattatcgta tccatatatg tatctatcgt
     1621 atagagtaaa ttttttgttg tcataaatat atatgtcttt tttaatgggg tgtatagtac
     1681 cgctgcgcat agtttttctg taatttacaa cagtgctatt ttctggtagt tcttcggagt
     1741 gtgttgcttt aattattaaa tttatataat caatgaattt gggatcgtcg gttttgtaca
     1801 atatgttgcc ggcatagtac gcagcttctt ctagttcaat tacaccattt tttagcagca
     1861 ccggattaac ataactttcc aaaatgttgt acgaaccgtt aaacaaaaac agttcacctc
     1921 ccttttctat actattgtct gcgagcagtt gtttgttgtt aaaaataaca gccattgtaa
     1981 tgagacgcac aaactaatat cacaaactgg aaatgtctat caatatatag ttgctgatat
     2041 aacttcgtat agcatacatt atacgaagtt atctggttga aacgtacccg tagtggctat
     2101 ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     2161 ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
     2221 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     2281 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     2341 ccttccgtgt ttcagttagc ctcccccatc tcccgctatt actcgagtca ctgggagccg
     2401 gagtggcggg cctcggcgtg ctcgtactgc tccaccacgg tgtagtcctc gttgtggttg
     2461 gtgatgtcca gcttggagtc cacgtagtgg ttgccgggca gctgcacggg cttcttggcc
     2521 ttgtacacgg tcttgaagtc gcaggtgtag tggccgccgt ccttcagctt cagggcgtgg
     2581 gagatctcgc ccttcagcac gccgtcctgg gggtacagct tctcggtgga gggctcccag
     2641 ccggcagtct tcttctgcat tacggggccg tcggcgggga agttcacgcc cttgaacttc
     2701 accttgtaga tgaaggtgcc gtcctgcagg gaggagtcct gctgcacctc caccacgccg
     2761 ccgtcctcga agttcatgga gcgctcccag gtgaagccct cggggaagga cagcttcatg
     2821 tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtactg gaactggggg
     2881 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcacctgcag cttggcggtc
     2941 tgggtgccct cgtagggctt gccctcgccc tcgccctcga tctcgaagta gtggccgttc
     3001 acggagccct ccatgcgcac cttgaactgc atgaactcct tgatgacgtc ctcggtgttg
     3061 tccatggtag agtgattgta aataaaatgt aatttacagt atagtatttt aattaatata
     3121 caaatgattt gataataatt cttatttaac tataatatat tgtgttgggt tgaattaaag
     3181 gtccgtatac tagtatcgat tcgcgaccta ctccggaata ttaatagagt tgctgatatc
     3241 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
     3301 acagttttgt aataaaaaaa cctataaata cggatctacc atggactgga gccacccgca
     3361 gttcgaaaaa tccgcggtcg gcctgaacga catcttcgag gctcagaaaa tcgaatggca
     3421 cgaaggtgga ggaggttctg gagagaacct ctacttccag ggaggcggcc gcatgaagag
     3481 gcgagctggt ctgggcggtt ccatgagatc tgttgttggc tttctctctc aacgtggact
     3541 gcacggagat ccactgttga cgcaagactt ccaacgtcgt cgattgaggg gttgccgtaa
     3601 cctatataag aaggatttgc ttggtcattt cggctgcgtg aacgcaatcg aatttagcaa
     3661 taacggtggt caatggcttg tcagcggtgg agatgaccgc cgtgtgttgc tctggcacat
     3721 ggaacaggct atccattcac gcgtcaagcc cattcagctt aaaggcgaac accattcaaa
     3781 cattttctgc ctcgccttta acagcggaaa cacgaaggta tttagcggcg gcaacgatga
     3841 gcaagtgata ctacacgacg ttgaatcatc agaaactctt gacgtctttg ctcacgaaga
     3901 tgcagtttac ggtctgtcag tgtcgcccgt gaacgataac atctttgctt cgtcaagcga
     3961 cgacggtagg gttttgattt gggacatccg tgagtcgcca cacggcgagc ccttctgtct
     4021 cgctaactac ccatccgctt ttcacagtgt catgttcaat ccagtcgaac ctagattgct
     4081 ggctactgct aatagtaaag agggagtggg cctctgggat atccgtaaac cgcagagttc
     4141 cttgcttcga tacggtggca acctgagtct acaatccgct atgtctgtcc gttttaatag
     4201 caacggaact caattgctcg ctctccgaag acgccttcca ccggtccttt atgacatcca
     4261 ctcgcgcctg ccggtgttcc aattcgataa ccagggttac ttcaattcct gcaccatgaa
     4321 aagttgctgc tttgcgggcg accgtgatca atacattctg tctggatcgg acgattttaa
     4381 cctctatatg tggcgcatac cagccgaccc ggaagccggt ggtatcggac gcgtagttaa
     4441 cggagctttt atggtcctga aaggccaccg ttccattgtt aaccaagtgc gtttcaaccc
     4501 tcacacgtac atgatctgca gctctggagt cgagaaaatt atcaaaatat ggtcccccta
     4561 caagcaacca ggttgcaccg gtgacctgga tggcaggatc gaagacgatt cacgctgttt
     4621 gtacacccat gaagaataca tttcccttgt actcaactca ggctcaggcc tgtcccacga
     4681 ctacgcgaac cagtcggtgc aggaagaccc ccgcatgatg gccttcttcg acagcctagt
     4741 caggcgcgag atagagggtt ggtcatctga ttctgactcc gacctatctg agtcaaccat
     4801 tctccaactg cacgccggcg tgtcagagcg atctggttac acggattctg aatcctcggc
     4861 gtccctgcct aggtctccac cgccaacagt ggacgaatct gctgacaatg cgttccactt
     4921 gggtccactg cgcgtcacaa caactaacac tgtagcttcg actccaccta ctccgacgtg
     4981 cgaagacgct gcgtcccgtc aacaaaggtt gtccgcgctg agacgatacc aggacaagag
     5041 attgcttgcg ttgtcgaacg agagtgacag tgaagaaaac gtgtgtgaag tcgaactgga
     5101 cacagatctc ttcccccgcc cgaggtcccc ctcgccagaa gacgaatcat catcgtcctc
     5161 gtcgtcttcc agcagcgagg acgaggaaga actaaacgaa agaagggcca gcacatggca
     5221 gcgcaacgcc atgaggcgta ggcaaaagac aacgagggaa gacaaacctt cagcgccgat
     5281 aaaaccaacc aacacttaca tcggagaaga taactatgat tacccacaga tcaaagttga
     5341 tgacctgtca tcatcgccaa ccagctcacc cgaaagatcc actagtactc tagagatcca
     5401 acctagcaga gccagtccaa cttcggacat tgagtcggtt gaaaggaaga tctacaaagc
     5461 ctataagtgg ttgcgatata gttatatttc ctactctaac aacaaggacg gtgaaacctc
     5521 tttggtcaca ggcgaagcag acgagggtcg tgccggaacc tctcacaagg acaaccccgc
     5581 tccttcatcc tcgaaagagg cttgcttgaa catcgccatg gcccaacgta accaagatct
     5641 tccccctgag ggctgttcca aggacacgtt caaggaagaa acaccccgca cgccgtccaa
     5701 cggacctggt catgagcatt ccagtcacgc gtgggcggaa gttccagagg gaacatctca
     5761 agatacaggt aattcgggtt ccgtcgagca cccgttcgag actaagaagc taaacggtaa
     5821 ggctctctct tctcgcgcgg aagagccgcc gtcaccccca gtgcctaagg cgtcgggtag
     5881 cacactcaac tcaggctctg gaaattgccc taggacacaa agcgacgaca gcgaagagag
     5941 gtccctggag acaatctgcg cgaaccacaa caacggaaga ctgcaccccc gtccacccca
     6001 cccccacaat aacggtcaaa acctgggaga actagaggtt gtagcctatt caagcccagg
     6061 tcactctgac accgatcgcg acaatagctc tctgactggt actctcttgc ataaagattg
     6121 ctgtggctct gagatggcct gtgagactcc caacgccggt actagggaag atccaacaga
     6181 cactcccgcc acagactcat cacgtgcagt gcatggccac tcaggactta agagacagcg
     6241 tatagagctt gaggacaccg actccgaaaa ctcgtcctct gaaaagaaac tcaagacctg
     6301 aggtaccccc ggacggccgc ttaattaatt gatccgggtt attagtacat ttattaagcg
     6361 ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta
     6421 aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta
     6481 tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca
     6541 aacaagggtt gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc
     6601 acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg
     6661 ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca
     6721 ctgtcgttag tgtacaattg actcgacgta aacacgttaa ataaagcttg gacatattta
     6781 acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta
     6841 gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct
     6901 aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt
     6961 ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc
     7021 gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga
     7081 gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc
     7141 ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca
     7201 gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga
     7261 cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt
     7321 gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt
     7381 ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc
     7441 gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc
     7501 gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat
     7561 tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta
     7621 tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat
     7681 tgtctcaagc tcggatcgat cccgcacgcc gataacaagc cttttcattt ttactacagc
     7741 attgtagtgg cgagacactt cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc
     7801 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     7861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8041 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8161 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     8221 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8281 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8341 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8401 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8461 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     8521 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8641 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8701 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8761 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8821 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8881 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8941 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9001 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9061 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9121 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9181 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9241 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9301 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9361 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9421 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9481 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9541 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9601 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaa
//
