LOCUS       sequence-494104-       10276 bp    DNA     circular SYN 01-DEC-2025
DEFINITION  Control plasmid for EKAREN5 without mTurquioise2 cassette 
            (expressing YFP only).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Weisheng Z, Nakayama J, Inomata Y, Higashiyama S, Hiratsuka T
  TITLE     A sensitive ERK fluorescent probe reveals the significance of 
            minimal EGF-induced transcription.
  JOURNAL   Cell Struct Funct. 2024 Dec 18. doi: 10.1247/csf.24070.
  PUBMED    39694501
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2024 Dec 18. doi: 10.1247/csf.24070."
COMMENT     Sequence Label: EKAREN5-YFPonly
FEATURES             Location/Qualifiers
     source          1..10276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    186..738
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(330..347)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     557..576
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             765..1364
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(765..784)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1261..1281
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2256..2489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2561..2682
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2598..2617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2652..2671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2722..2857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2877..2896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2878..2896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2906..2923)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2906..2922)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2915..2937)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3064..3519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3151..3170)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3361..3382
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3545..3649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3650..3718,3719..4510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3868..3887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4681..5269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5170..5189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5423..5440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5557..5578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5593..5598,5599..5616,5617..5623)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5631..5647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5636..5658
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5655..5671
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5655..5671
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5690..5710
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5692..5710
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5738..5964
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5965..6145
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6192..6317
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6810..7043
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7228..7272
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7534..7651
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7679..8841
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7910..8832
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8789..8809
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(8923..8925,8926..8928,8929..9636)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     primer_bind     complement(8968..8989)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9229..9248)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             10234..10254
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctagccta gtgccatttg ttcagtggtt cgtagggctt tcccccactg tttggctttc
       61 agttatatgg atgatgtggt attgggggcc aagtctgtac agcatcttga gtcccttttt
      121 accgctgtta ccaattttct tttgtctttg ggtaaacccg ggaattccgc ccctctcccc
      181 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
      241 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
      301 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
      361 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
      421 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
      481 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
      541 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
      601 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
      661 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
      721 aaaaacacga tgataatatg gatatggcca caacggtcgc caccatgacc gagtacaagc
      781 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
      841 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
      901 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
      961 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     1021 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     1081 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     1141 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     1201 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     1261 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     1321 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     1381 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatg tattcaatct aagcaggctt
     1441 tcactttctc gccaacttac aaggcctttc tgtgtaaaca atacctgaac ctttaccccg
     1501 ttgcccggca acggccaggt ctgtgccaag tgtttgctga cgcaaccccc actggctggg
     1561 gcttggtcat gggccatcag cgcatgcgtg gaaccttttc ggctcctctg ccgatccata
     1621 ctgcggaact cctagccgct tgttttgctc gcagcaggtc tggagcaaac attatcggga
     1681 ctgataactc tgttgtccta tcccgcaaat atacatcgtt tccatggctg ctaggctgtg
     1741 ctgccaactg gatcctgcgc gggacgtcct ttgtttacgt cccgtcggcg ctgaatcctg
     1801 cggacgaccc ttctcggggt cgcttgggac tctctcgtcc ccttctccgt ctgccgttcc
     1861 gaccgaccac ggggcgcacc tctctttacg cggactcccc gtctgtgcct tctcatctgc
     1921 cggaccgtgt gcacttcgct tcacctctgc acgtcgcatg gagaccaccg tgaacgccca
     1981 ccaaatattg cccaaggtct tacataagag gactcttgga ctctcagcaa tgtcaacgac
     2041 cgaccttgag gcatacttca aagactgttt gtttaaagac tgggaggagt tgggggagga
     2101 gattaggtta aaggtctttg tactaggagg ctgtaggcat aaattggtct gcgcaccagc
     2161 accatggcgc aatcactaga gcggggtacc tttaagacca atgacttaca aggcagctgt
     2221 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     2281 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     2341 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2401 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2461 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     2521 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     2581 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2641 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     2701 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2761 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     2821 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     2881 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2941 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3061 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3121 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3181 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3241 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3301 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3361 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3421 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3481 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     3541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4201 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4261 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5341 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5401 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5461 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     5521 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     5581 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     5641 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     5701 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     5761 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     5821 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     5881 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     5941 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     6001 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6061 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6121 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     6181 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     6241 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     6301 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     6361 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     6421 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     6481 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     6541 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     6601 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     6661 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     6721 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     6781 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     6841 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     6901 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     6961 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     7021 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     7081 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     7141 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     7201 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     7261 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     7321 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     7381 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     7441 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     7501 attcgattag tgaacggatc tcgacggtat cggttttaaa agaaaagggg ggattggggg
     7561 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     7621 acaaaaacaa attacaaaaa ttcaaaattt tatcgatcat atgactagag cggccttagg
     7681 ctccggtgcg cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     7741 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     7801 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     7861 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     7921 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     7981 ttccacctgg ctccagtacg tgattcttga tcccgagctg gagccagggg cgggccttgc
     8041 gctttaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     8101 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     8161 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     8221 gcgggccagg atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     8281 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     8341 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     8401 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     8461 tggccgcttc ccggccctgc tccagggggc tcaaaatgga ggacgcggcg ctcgggagag
     8521 cgggcgggtg agtcacccac acaaaggaaa ggggcctttc cgtcctcagc cgtcgcttca
     8581 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctg gagcttttgg
     8641 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     8701 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     8761 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     8821 cttccatttc aggtgtcgtg attaattaaa cgcgtaccga tagcttcgaa ttctgcagtc
     8881 gacgatatca ctagtctcga gcattttggc aaagaattcg gcatggtgag caagggcgag
     8941 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     9001 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     9061 cttctatgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
     9121 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     9181 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     9241 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     9301 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     9361 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     9421 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     9481 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     9541 gccctgttca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcctgacc
     9601 gccgccggga tcactgaggg catgaacgag ctgtacctcg agatggcgga cgaggagaag
     9661 ctgccgcccg gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac
     9721 cacatcacta acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa
     9781 aacgggcagg gggagcctgc caggggtacc agtgctggtg gtagtgctgg tggtagtgct
     9841 ggtggtagtg ctggtggtag tgctggtggt tccggcagtg ctggtggtag tgctggtggt
     9901 agtaccagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
     9961 ggtggttccg gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg tggtagtgct
    10021 ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt
    10081 agtgctggtg gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
    10141 ggtggtagtg ctggtggttc cggaccagat gtccctagaa ctccagtgga tccagcatgg
    10201 ctgtcattcc aatttccggg cggccgctct agacctaaga agaagcgtaa ggtgtaagtc
    10261 gacggtcgag tcgaga
//
