LOCUS       sequence-494105-       10984 bp    DNA     circular SYN 01-DEC-2025
DEFINITION  Control plasmid for EKAREN5 and EKAREN5-gl (synonymous variant YPet 
            - mTurquoise2 pair).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Weisheng Z, Nakayama J, Inomata Y, Higashiyama S, Hiratsuka T
  TITLE     A sensitive ERK fluorescent probe reveals the significance of 
            minimal EGF-induced transcription.
  JOURNAL   Cell Struct Funct. 2024 Dec 18. doi: 10.1247/csf.24070.
  PUBMED    39694501
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2024 Dec 18. doi: 10.1247/csf.24070."
COMMENT     Sequence Label: EKAREN5-y
FEATURES             Location/Qualifiers
     source          1..10984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        294..398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(399..467,468..1259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(617..636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1430..2018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1919..1938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2172..2189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2306..2327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2342..2347,2348..2365,2366..2372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2380..2396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2385..2407
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2404..2420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2404..2420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2439..2459
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2441..2459
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2487..2713
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2714..2894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2941..3066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3559..3792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3977..4021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4283..4400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4428..5590
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4659..5581
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5538..5558
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5672..6382
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELY"
     CDS             7691..7711
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7919..8471
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8063..8080)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8290..8309
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8498..9097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8498..8517)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8994..9014
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9989..10222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10294..10415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10331..10350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10385..10404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10455..10590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10610..10629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10611..10629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10639..10656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10639..10655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10648..10670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(10797..10984,1..268)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10884..10903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
       61 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
      121 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
      181 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
      241 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
      301 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      361 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      421 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      481 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      541 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      601 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      661 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      721 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      781 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
      841 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
      901 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
      961 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1021 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1081 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1141 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1201 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1261 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1321 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1381 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1441 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1501 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1561 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1621 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1681 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1741 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1801 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1861 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1921 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1981 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2041 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2101 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2161 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2221 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2281 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2341 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2401 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     2461 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     2521 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     2581 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     2641 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     2701 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2881 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     2941 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     3001 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     3061 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     3121 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     3181 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     3241 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     3301 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     3361 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     3421 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     3481 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     3541 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     3601 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     3661 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     3721 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     3781 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     3841 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     3901 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     3961 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     4021 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     4081 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     4141 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     4201 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     4261 gaacggatct cgacggtatc ggttttaaaa gaaaaggggg gattgggggg tacagtgcag
     4321 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     4381 ttacaaaaat tcaaaatttt atcgatcata tgactagagc ggccttaggc tccggtgcgc
     4441 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     4501 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     4561 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     4621 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     4681 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     4741 tccagtacgt gattcttgat cccgagctgg agccaggggc gggccttgcg ctttaggagc
     4801 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     4861 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     4921 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccagga
     4981 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     5041 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     5101 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     5161 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     5221 cggccctgct ccagggggct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     5281 gtcacccaca caaaggaaag gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     5341 ggagtaccgg gcgccgtcca ggcacctcga ttagttctgg agcttttgga gtacgtcgtc
     5401 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     5461 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     5521 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     5581 ggtgtcgtga ttaattaaac gcgtaccgat agcttcgaat tctgcagtcg acgatatcac
     5641 tagtctcgag cattttggca aagaattcgg catgtctaaa ggtgaagaat tattcactgg
     5701 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     5761 cggtgaaggt gaaggtgatg ctacgtacgg taaattgacc ttaaaattac tctgtactac
     5821 tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg gtgttcaatg
     5881 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     5941 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     6001 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     6061 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     6121 ttacatcact gctgacaaac aaaagaatgg tatcaaagct aacttcaaaa ttagacacaa
     6181 cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc caattggtga
     6241 tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct tattcaaaga
     6301 tccaaacgaa aagagagacc acatggtctt gttagaattt ttgactgctg ctggtattac
     6361 cgagggtatg aatgaattgt acctcgagat ggcggacgag gagaagctgc cgcccggctg
     6421 ggagaagcgc atgagccgca gctcaggccg agtgtactac ttcaaccaca tcactaacgc
     6481 cagccagtgg gagcggccca gcggcaacag cagcagtggt ggcaaaaacg ggcaggggga
     6541 gcctgccagg ggtaccagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     6601 tggtagtgct ggtggttccg gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg
     6661 tggtagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gttccggcag
     6721 tgctggtggt agtgctggtg gtagtaccag tgctggtggt agtgctggtg gtagtgctgg
     6781 tggtagtgct ggtggtagtg ctggtggttc cggcagtgct ggtggtagtg ctggtggtag
     6841 taccagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg
     6901 tggttccgga ccagatgtcc ctagaactcc agtggatcca gcatggctgt cattccaatt
     6961 tccgggcggc cgcatggtga gtaaagggga agaactgttt actggcgtgg tgccaattct
     7021 ggtcgaactg gatggagatg taaatgggca taaatttagt gtgtctggag aaggggaagg
     7081 agacgctaca tatgggaaac tgacactgaa atttatttgt actactggaa aactgcctgt
     7141 gccttggcct acactcgtga caaccctgtc ttggggagtg cagtgttttg cccggtatcc
     7201 tgatcatatg aaacagcatg attttttcaa atctgccatg cctgagggat atgtccagga
     7261 acggacaatt ttctttaaag atgatgggaa ttataaaaca cgggccgaag tgaaatttga
     7321 aggggataca ctggtgaatc ggattgaact gaaaggaatt gattttaaag aagatgggaa
     7381 tattctgggc cataaactgg aatataatta tttcagcgat aatgtctaca ttacagccga
     7441 taaacagaag aatgggatta aagccaattt taaaattcgg cataatattg aagatggagg
     7501 ggtgcagctc gccgatcatt atcagcagaa tacacctatt ggggatgggc ctgtgctgct
     7561 gcctgataat cattatctga gcacacagtc tgccctgagc aaggacccta atgaaaagcg
     7621 ggaccatatg gtcctgctgg aatttctgac tgccgccggc attacactcg ggatggatga
     7681 actgtctaga cctaagaaga agcgtaaggt gtaagtcgac ggtcgagtcg agaagctagc
     7741 ctagtgccat ttgttcagtg gttcgtaggg ctttccccca ctgtttggct ttcagttata
     7801 tggatgatgt ggtattgggg gccaagtctg tacagcatct tgagtccctt tttaccgctg
     7861 ttaccaattt tcttttgtct ttgggtaaac ccgggaattc cgcccctctc cccccctaac
     7921 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     7981 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     8041 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     8101 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     8161 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     8221 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     8281 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     8341 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     8401 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     8461 cgatgataat atggatatgg ccacaacggt cgccaccatg accgagtaca agcccacggt
     8521 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8581 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8641 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8701 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8761 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8821 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8881 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8941 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9001 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9061 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     9121 cccgaccgaa aggagcgcac gaccccatgc atgtattcaa tctaagcagg ctttcacttt
     9181 ctcgccaact tacaaggcct ttctgtgtaa acaatacctg aacctttacc ccgttgcccg
     9241 gcaacggcca ggtctgtgcc aagtgtttgc tgacgcaacc cccactggct ggggcttggt
     9301 catgggccat cagcgcatgc gtggaacctt ttcggctcct ctgccgatcc atactgcgga
     9361 actcctagcc gcttgttttg ctcgcagcag gtctggagca aacattatcg ggactgataa
     9421 ctctgttgtc ctatcccgca aatatacatc gtttccatgg ctgctaggct gtgctgccaa
     9481 ctggatcctg cgcgggacgt cctttgttta cgtcccgtcg gcgctgaatc ctgcggacga
     9541 cccttctcgg ggtcgcttgg gactctctcg tccccttctc cgtctgccgt tccgaccgac
     9601 cacggggcgc acctctcttt acgcggactc cccgtctgtg ccttctcatc tgccggaccg
     9661 tgtgcacttc gcttcacctc tgcacgtcgc atggagacca ccgtgaacgc ccaccaaata
     9721 ttgcccaagg tcttacataa gaggactctt ggactctcag caatgtcaac gaccgacctt
     9781 gaggcatact tcaaagactg tttgtttaaa gactgggagg agttggggga ggagattagg
     9841 ttaaaggtct ttgtactagg aggctgtagg cataaattgg tctgcgcacc agcaccatgg
     9901 cgcaatcact agagcggggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9961 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
    10021 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10201 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
    10261 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
    10321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
    10441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    10561 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
    10621 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    10681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    10741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
    10801 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10861 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10921 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10981 ttta
//
