LOCUS       sequence-494126-       10987 bp    DNA     circular SYN 01-DEC-2025
DEFINITION  Control plasmid for EKAREN5 and EKAREN5-gl (original YPet - 
            mTurquoise-GL pair).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Weisheng Z, Nakayama J, Inomata Y, Higashiyama S, Hiratsuka T
  TITLE     A sensitive ERK fluorescent probe reveals the significance of 
            minimal EGF-induced transcription.
  JOURNAL   Cell Struct Funct. 2024 Dec 18. doi: 10.1247/csf.24070.
  PUBMED    39694501
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2024 Dec 18. doi: 10.1247/csf.24070."
COMMENT     Sequence Label: EKAREN5-c
FEATURES             Location/Qualifiers
     source          1..10987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    77..194
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        222..1384
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          453..1375
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1332..1352
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1466..1468,1469..1471,1472..2179)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             join(2771..2773,2774..2776,2777..3289)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             3488..3508
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3716..4268
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3860..3877)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4087..4106
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4295..4894
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4295..4314)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4791..4811
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5786..6019
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6091..6212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6128..6147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6182..6201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6252..6387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6407..6426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6408..6426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6436..6453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6436..6452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6445..6467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6594..7049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6681..6700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6891..6912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7075..7179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7180..7248,7249..8040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7398..7417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8211..8799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8700..8719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8953..8970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9087..9108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9123..9128,9129..9146,9147..9153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9161..9177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9166..9188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9185..9201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9185..9201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9220..9240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9222..9240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9268..9494
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9495..9675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9722..9847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10340..10573
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10758..10802
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
       61 atctcgacgg tatcggtttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      121 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      181 aaattcaaaa ttttatcgat catatgacta gagcggcctt aggctccggt gcgcgtcagt
      241 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
      301 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      361 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      421 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      481 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctccagt
      541 acgtgattct tgatcccgag ctggagccag gggcgggcct tgcgctttag gagccccttc
      601 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      661 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      721 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aggatctgca
      781 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      841 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      901 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      961 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     1021 tgctccaggg ggctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     1081 cacacaaagg aaaggggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1141 ccgggcgccg tccaggcacc tcgattagtt ctggagcttt tggagtacgt cgtctttagg
     1201 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1261 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1321 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1381 gtgattaatt aaacgcgtac cgatagcttc gaattctgca gtcgacgata tcactagtct
     1441 cgagcatttt ggcaaagaat tcggcatggt gagcaagggc gaggagctgt tcaccggggt
     1501 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1561 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagcttctat gcaccaccgg
     1621 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
     1681 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1741 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1801 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1861 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1921 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1981 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2041 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctgt tcaaagaccc
     2101 caacgagaag cgcgatcaca tggtcctgct ggagttcctg accgccgccg ggatcactga
     2161 gggcatgaac gagctgtacc tcgagatggc ggacgaggag aagctgccgc ccggctggga
     2221 gaagcgcatg agccgcagct caggccgagt gtactacttc aaccacatca ctaacgccag
     2281 ccagtgggag cggcccagcg gcaacagcag cagtggtggc aaaaacgggc agggggagcc
     2341 tgccaggggt accagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     2401 tagtgctggt ggttccggca gtgctggtgg tagtgctggt ggtagtacca gtgctggtgg
     2461 tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtt ccggcagtgc
     2521 tggtggtagt gctggtggta gtaccagtgc tggtggtagt gctggtggta gtgctggtgg
     2581 tagtgctggt ggtagtgctg gtggttccgg cagtgctggt ggtagtgctg gtggtagtac
     2641 cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta gtgctggtgg
     2701 ttccggacca gatgtcccta gaactccagt ggatccagca tggctgtcat tccaatttcc
     2761 gggcggccgc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2821 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2881 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2941 ctggcccacc ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga
     3001 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3061 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3121 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3181 cctggggcac aagctggagt acaactacat cagcgggaac gtctatatca ccgccgacaa
     3241 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     3301 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3361 cgacaaccac tacctgagca cccagtccgc cttaagcaaa gaccccaacg agaagcgcga
     3421 tcacatggtc ctgctggagt tcttgaccgc cgccgggatc actctcggca tggacgagct
     3481 gtctagacct aagaagaagc gtaaggtgta agtcgacggt cgagtcgaga agctagccta
     3541 gtgccatttg ttcagtggtt cgtagggctt tcccccactg tttggctttc agttatatgg
     3601 atgatgtggt attgggggcc aagtctgtac agcatcttga gtcccttttt accgctgtta
     3661 ccaattttct tttgtctttg ggtaaacccg ggaattccgc ccctctcccc ccctaacgtt
     3721 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     3781 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     3841 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     3901 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     3961 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4021 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4081 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4141 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4201 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4261 tgataatatg gatatggcca caacggtcgc caccatgacc gagtacaagc ccacggtgcg
     4321 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4381 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4441 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4501 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4561 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4621 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4681 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     4741 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     4801 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     4861 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     4921 gaccgaaagg agcgcacgac cccatgcatg tattcaatct aagcaggctt tcactttctc
     4981 gccaacttac aaggcctttc tgtgtaaaca atacctgaac ctttaccccg ttgcccggca
     5041 acggccaggt ctgtgccaag tgtttgctga cgcaaccccc actggctggg gcttggtcat
     5101 gggccatcag cgcatgcgtg gaaccttttc ggctcctctg ccgatccata ctgcggaact
     5161 cctagccgct tgttttgctc gcagcaggtc tggagcaaac attatcggga ctgataactc
     5221 tgttgtccta tcccgcaaat atacatcgtt tccatggctg ctaggctgtg ctgccaactg
     5281 gatcctgcgc gggacgtcct ttgtttacgt cccgtcggcg ctgaatcctg cggacgaccc
     5341 ttctcggggt cgcttgggac tctctcgtcc ccttctccgt ctgccgttcc gaccgaccac
     5401 ggggcgcacc tctctttacg cggactcccc gtctgtgcct tctcatctgc cggaccgtgt
     5461 gcacttcgct tcacctctgc acgtcgcatg gagaccaccg tgaacgccca ccaaatattg
     5521 cccaaggtct tacataagag gactcttgga ctctcagcaa tgtcaacgac cgaccttgag
     5581 gcatacttca aagactgttt gtttaaagac tgggaggagt tgggggagga gattaggtta
     5641 aaggtctttg tactaggagg ctgtaggcat aaattggtct gcgcaccagc accatggcgc
     5701 aatcactaga gcggggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5761 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5821 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5881 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5941 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6001 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6061 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6121 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6181 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     6241 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6301 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6361 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     6421 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6481 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6541 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     6601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6781 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6841 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6901 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6961 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7021 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     7081 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7141 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7201 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7261 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7321 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7381 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     7441 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7501 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7561 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7621 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7681 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7741 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7801 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7861 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7921 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7981 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8041 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8341 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9001 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9061 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9181 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     9241 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     9301 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     9361 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     9421 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     9481 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9661 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     9721 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     9781 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     9841 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     9901 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     9961 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
    10021 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
    10081 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
    10141 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
    10201 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    10261 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    10321 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    10381 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    10441 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    10501 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    10561 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    10621 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    10681 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    10741 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    10801 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    10861 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    10921 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    10981 ggcccga
//
