LOCUS       sequence-494128-       12873 bp    DNA     circular SYN 01-DEC-2025
DEFINITION  Overexpression of HyperdLbCas12a in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Yang LZ, Min YH, Liu YX, Gao BQ, Liu XQ, Huang Y, Wang H, Yang L, 
            Liu ZJ, Chen LL
  TITLE     CRISPR-array-mediated imaging of non-repetitive and multiplex 
            genomic loci in living cells.
  JOURNAL   Nat Methods. 2024 Sep;21(9):1646-1657. doi: 
            10.1038/s41592-024-02333-3. Epub 2024 Jul 4.
  PUBMED    38965442
REFERENCE   2  (bases 1 to 12873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-024-02333-3"; journalName: "Nat Methods"; date: 
            "2024-09"; volume: "21"; issue: "9"; pages: "1646-1657"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3x...
FEATURES             Location/Qualifiers
     source          1..12873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      7170..7179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7176..7178,7179..7181,7182..7892)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7176..7178,7179..7181,7182..7892)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7221..7242)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7482..7501)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7829..7850
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7920..7967
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7986..8051
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8058..8625
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8219..8236)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8446..8465
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8645..9244
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8645..8664)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9141..9161
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9263..9851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9316..9336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9734..9745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9926..10159
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10278..10296)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10364..10468
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10469..10537,10538..11329)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10687..10706)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11500..12088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11989..12008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12242..12259
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12499..12520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12535..12540,12541..12558,12559..12565)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12573..12589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12578..12600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12597..12613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12597..12613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12663..12682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagctca aagctggaga
     3481 aattcaccaa ctgttatagc ctgtctaaga ccctgcgctt caaggcaatc ccagtgggca
     3541 agacacaaga gaacattgac aacaaacggc tcctggtgga ggatgagaag agggctgaag
     3601 attacaaggg cgttaagaag ctgctggata ggtactatct gtcattcatc aacgatgtcc
     3661 tccacagtat caagctgaag aatctgaaca attacatttc tctgttccgg aagaagacac
     3721 ggaccgagaa ggagaacaaa gagctggaga atctggagat caacctgagg aaagaaatag
     3781 ctaaggcttt caaagggaac gagggttaca agtccctgtt caagaaagac attatcgaga
     3841 ctattctgcc tgagttcctg gacgataaag atgagatcgc cctcgtcaat tccttcaatg
     3901 ggtttaccac agcctttacc ggcttcttca ggaatagaga gaatatgttc tctgaagagg
     3961 ccaaatccac tagcatcgcc tttcgctgca taaacgagaa cctgactagg tacatcagca
     4021 atatggacat ctttgagaaa gtcgatgcca tattcgacaa acatgaggtg caggagatta
     4081 aggagaagat cctgaactca gattacgatg tcgaagattt cttcgaggga gagttcttca
     4141 acttcgtgct cacacaagag ggcattaggg tgtacaatgc aatcattgga gggttcgtga
     4201 cagagagtgg cgagaagata aagggcctga acgagtatat caacctctac aaccagaaaa
     4261 ccaagcagaa actgcctaag ttcaagccac tgtacaaaca agtgctctca gatagggaaa
     4321 gcctgagctt ctacggtagg gggtatacat cagatgaaga agtgctcgaa gtgttccgca
     4381 acaccctcaa taagaacagt gaaatcttct cttcaatcaa gaagctggag aaactgttca
     4441 agaatttcga tgagtactcc tctgccggaa tctttgtgaa gaatggccct gcaatatcca
     4501 ctattagcaa aaggatcttt ggcgagtgga acgttatcag ggataagtgg aatgccgagt
     4561 acgatgatat tcatctcaag aagaaagccg tggttacaga gaaatacgag gatgatagac
     4621 gcaagagctt taagaagatt ggtagcttct ctctcgaaca gctgcaggag tacgccgacg
     4681 ctgacctgtc agtcgtggag aaactcaagg agatcataat ccagaaggtg gatgaaatct
     4741 acaaagtgta tggaagctct gagaaactct tcgatgcaga ctttgttctg gagaagagtc
     4801 tgaagaagaa cgacgcagtg gttgctatca tgaaggacct gctggattct gttaagtctt
     4861 tcgagaatta cattaaggca ttctttggtg aagggaagga gacaaatagg gacgagagct
     4921 tctatggcga ctttgttctg gcctacgaca tcctcctcaa ggttgaccac atctatgacg
     4981 ctatacggaa ttacgttacc cagaagccct atagcaaaga caagttcaag ctgtatttcc
     5041 agaatccaca gtttatgggt gggtgggata aagacaaaga aacagattac agggccacta
     5101 tcctgcggta cggcagcaaa tactatctgg ctatcatgga taagaagtac gccaaatgcc
     5161 tccagaagat cgacaaggac gacgtgaacg gtaactacga gaagatcaat tacaagctcc
     5221 tgccaggacc taacaagatg ctgcccaagg tgttcttctc caagaaatgg atggcctact
     5281 ataacccaag cgaggacatt cagaagatat acaagaatgg gacattcaag aagggcgata
     5341 tgttcaacct caacgactgc cacaagctga ttgatttctt caaggatagc atttctcgct
     5401 atcccaagtg gtctaatgca tacgatttca acttcagcga gactgagaag tacaaagaca
     5461 tcgctggctt ctaccgggag gtggaagagc aaggctataa ggtgtcattc gaatccgctt
     5521 ctaagaagga agtggataag ctcgtggaag agggtaagct gtacatgttc cagatataca
     5581 acaaagactt cagcgataag agccacggca ctccaaacct ccatactatg tatttcaagc
     5641 tgctgtttga cgagaacaac cacggacaga ttaggctgtc aggaggcgca gaactcttca
     5701 tgcgcagagc ttcactgaag aaggaggaac tcgttgtcca cccagccaat agccctatag
     5761 ccaataagaa tccagacaat cctaagaaaa ccactactct gtcttacgat gtgtataagg
     5821 ataagagatt ctctgaagat cagtacgaac tgcacatacc cattgccatt aacaagtgcc
     5881 ctaagaacat cttcaagatt aacacagagg ttagagtgct cctgaaacac gacgataacc
     5941 cttatgttat aggcattgct cgcggagaga gaaacctgct gtacatcgtc gtggtggacg
     6001 gcaaaggcaa catcgtggaa cagtacagtc tcaatgaaat cattaacaat ttcaacggaa
     6061 tccgcattaa gaccgactac cattctctcc tcgacaagaa ggagaaagaa aggttcgaag
     6121 caagacagaa ttggacaagt atagagaata tcaaagaact gaaggctggg tacatctctc
     6181 aggttgtgca caagatatgt gagctggtgg agaagtacga cgctgttatc gccctcgcgg
     6241 acctgaatag cggcttcaag aactccaggg tgaaggtgga gaagcaggtg tatcagaagt
     6301 tcgagaagat gctgatcgac aagctcaact atatggtgga caagaaatcc aatccttgcg
     6361 ctactggtgg agccctgaag ggctatcaaa tcaccaataa gttcgaatct ttcaagtcta
     6421 tgagcaccca gaatggcttc atcttctaca tacccgcatg gctgacatcc aagattgatc
     6481 cctctaccgg atttgttaat ctgctcaaga ctaagtacac ctctattgct gactcaaaga
     6541 agttcatatc atcatttgac cgcatcatgt acgtgccaga agaggacctg ttcgagtttg
     6601 ccctggatta caagaatttc tctcggactg acgccgacta catcaagaag tggaagctct
     6661 actcttatgg taatcggatt cgcatattcc gcaatcccaa gaagaataac gtgttcgatt
     6721 gggaggaagt ttgcctcacc agcgcttaca aggagctgtt caataagtat gggattaact
     6781 accagcaggg cgacataaga gccctgctgt gcgaacaatc tgataaggca ttctattcct
     6841 ctttcatggc actgatgtca ctgatgctgc aaatgcgcaa ttccatcacc ggaagaacag
     6901 acgtggcctt tctgatctct cctgtcaaga actcagatgg catcttctac gattcccgca
     6961 actatgaagc acaggagaat gctatcctgc ctaagaatgc cgatgcaaat ggagcctata
     7021 acatcgccag aaaggtcctc tgggccatag gacaattcaa gaaagctgaa gatgagaagc
     7081 tggacaaggt gaagatcgcc atttcaaaca aagagtggct cgaatatgct cagacctcag
     7141 tgaagcatag tctagaggcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
     7201 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     7261 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     7321 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     7381 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     7441 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     7501 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     7561 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     7621 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     7681 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     7741 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     7801 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     7861 ggatcactct cggcatggac gagctgtaca agtcgaattc taagccagga ttccatatga
     7921 agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaacaa cgcgtgcagc
     7981 taagcgacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
     8041 acgatgacaa gtaaggatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     8101 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     8161 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     8221 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     8281 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     8341 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     8401 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     8461 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     8521 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     8581 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataaggatc caccggagct
     8641 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8701 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8761 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8821 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     8881 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8941 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9001 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9061 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9121 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9181 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9241 ctgacgccca tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     9301 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     9361 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     9421 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     9481 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     9541 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     9601 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     9661 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9721 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9781 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9841 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     9901 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     9961 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
    10021 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10081 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10141 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
    10201 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
    10261 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
    10321 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    10381 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    10441 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    10501 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    10561 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    10621 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    10681 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
    10741 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10801 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10861 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    10921 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    10981 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    11041 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    11101 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    11161 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    11221 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    11281 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    11341 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    11401 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    11461 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    11521 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    11581 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    11641 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    11701 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    11761 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11821 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    11881 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    11941 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    12001 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    12061 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    12121 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    12181 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    12241 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    12301 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
    12361 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    12421 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
    12481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    12541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    12601 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
    12661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    12721 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    12781 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    12841 tatttgttaa atatgtacta caaacttagt agt
//
