LOCUS       sequence-494262-       22101 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  Expression of human VPS34 complex I + NRBF2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22101)
  AUTHORS   Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O,
            Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi 
            M, Ktistakis NT, Sachse C, Williams RL
  TITLE     Characterization of Atg38 and NRBF2, a fifth subunit of the 
            autophagic Vps34/PIK3C3 complex.
  JOURNAL   Autophagy. 2016 Nov;12(11):2129-2144. doi: 
            10.1080/15548627.2016.1226736. Epub 2016 Sep 14.
  PUBMED    27630019
REFERENCE   2  (bases 1 to 22101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/15548627.2016.1226736"; journalName: "Autophagy"; date: 
            "2016-11"; volume: "12"; issue: "11"; pages: "2129-2144"
COMMENT     Sequence Label: pMO15
FEATURES             Location/Qualifiers
     source          1..22101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        252..631
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        632..835
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    837..855
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    858..876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1033..1053
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1069..1242
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1243..1416
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1426..1446
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1477..1497
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1519..1539
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3136..3360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3402..3781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3782..3985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    3987..4005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4008..4026
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    5058..5282
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5324..5703
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5704..5907
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5952..5971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5952..5970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(6066..6068,6069..6071,6072..6785)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6066..6068,6069..6071,6072..6782)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6111..6132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6372..6391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6719..6740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(6795..6813)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6796..6813)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    6839..7063
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7105..7484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7485..7688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    7690..7708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7711..7729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    9256..9480
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9522..9901
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9902..10105
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    10107..10125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10128..10146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    12984..13208
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        13250..13629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13630..13833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    13835..13853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13856..13874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    18119..18343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      18389..18817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18476..18495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     18686..18707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(18826..18846)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        18831..19160
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      19011..19146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     19073..19092
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        19208..19255
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             19274..19648
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    19778..19899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(19815..19834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     19869..19888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(19948..19964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19948..19964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(19961..19983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    19972..19988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19996..20002,20003..20020,20021..20026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    20041..20062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(20179..20196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(20350..20938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20430..20449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(21109..21900,21901..21969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21732..21751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21970..22074)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagatg tacgggccag
      241 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      301 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      361 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      421 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      481 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      541 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      601 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      661 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      721 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      781 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
      841 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
      901 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      961 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccggat gggagcaggg
     1021 gcgggtgcta gcgagaacct gtacttccag ggcgcccacg atgaagccgt agacaacaaa
     1081 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
     1141 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
     1201 ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac
     1261 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa
     1321 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca
     1381 gaagctaaaa agctaaatga tgctcaggcg ccgaaagtag acgcggaaaa cctgtatttc
     1441 cagggaggcg gatcttctag accaactact gcctctgaaa atttgtactt tcaaggcgag
     1501 ttgaagacca ctgcttctga aaacttgtac ttccaaggtg agttactcga gggtgaattg
     1561 aagactgctg ctttggctca acacgacgaa gccatgacgg cgtctcccag tgggaaggga
     1621 gcccgggcgc tggaggctcc tggctgcggg ccccggccgc tcgcccggga cctggtggac
     1681 tccgtggacg atgcggaggg gctgtacgtg gctgtggagc gctgcccgct gtgcaacact
     1741 acccgccggc ggctgacctg cgccaaatgc gttcagagcg gcgatttcgt ctacttcgac
     1801 ggccgcgacc gggagaggtt tatcgacaag aaggaaaggt taagccgact taagagcaag
     1861 caagaagaat ttcagaaaga agtgttaaaa gctatggaag gaaaatggat aacagatcag
     1921 ttgagatgga aaataatgtc ctgcaagatg aggattgaac agttaaaaca aacaatatgt
     1981 aaaggaaatg aagaaatgga gaaaaattct gaaggccttc tcaaaaccaa ggaaaagaat
     2041 cagaagcttt acagtcgagc acaacggcac caagagaaaa aggagaagat tcagaggcat
     2101 aatcgcaaac ttggtgacct ggtagaaaaa aagaccattg acttaagaag tcattatgag
     2161 cgtctggcaa atcttcgacg atcccatata ttagagctca cctctgtcat ttttccaatc
     2221 gaggaagtaa agacgggtgt gagagacccc gcagatgtgt cttcagagag tgacagtgcc
     2281 atgacctcca gcactgtgag caagcttgct gaagcccgga ggacaactta cctctcagga
     2341 cgatgggtct gtgacgatca cagcggagac accagcatta gcattacagg gccttggatt
     2401 agcctcccta acaatgggga ctactctgcc tactacagct gggtggagga gaagaaaaca
     2461 acccaggggc ctgacatgga gcagagtaac cctgcctaca ccatcagtgc tgcgctgtgc
     2521 tatgcaactc agctggtcaa cattctgtct catatacttg atgtaaatct tcccaaaaag
     2581 ctctgcaaca gtgaattttg tggcgaaaat ctaagcaagc agaaatttac tcgagcagtg
     2641 aagaaactga atgcaaatat tctttacctt tgtttttctc agcatgtaaa tttagatcaa
     2701 ttacaaccac tgcataccct caggaatcta atgtacctgg tcagtccaag ctctgaacac
     2761 ctaggcaggt cagggccctt tgaagtacga gcagaccttg aggagtccat ggaatttgtg
     2821 gatcccggag ttgctggaga atcagatgag agcggagatg agcgcgtcag cgatgaagaa
     2881 accgacctgg gcacagactg ggagaacttg cctagtcccc ggttttgtga tatcccttcc
     2941 cagtctgtgg aagtctccca gagtcagagc acccaggcgt ccccacccat cgcgagcagc
     3001 agtgcaggtg ggatgatctc ctctgcagca gcctcggtga cctcctggtt taaagcttac
     3061 actggacacc gtagatccta acccaagctt aagtcgagtc tagagggccc gtttaaaccc
     3121 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3181 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3241 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3301 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3361 cttctgaggc ggaaagaacc agctggggct ctaggcgcgt tgacattgat tattgactag
     3421 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3481 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3541 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3601 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3661 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3721 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3781 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3841 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3901 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3961 gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga
     4021 tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     4081 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
     4141 acttaagctt atggaagtaa tggaaggacc cctcaacctg gctcatcaac agagcagacg
     4201 agcagaccgt ttattagctg caggcaaata cgaagaggct atttcttgtc acaaaaaggc
     4261 tgcagcatat ctttctgaag ccatgaagct gacacagtca gagcaggctc atctttcact
     4321 ggaattgcaa agggatagcc atatgaaaca gctcctcctc atccaagaga gatggaaaag
     4381 ggcccagcgt gaagaaagat tgaaagccca gcagaacaca gacaaggatg cagctgccca
     4441 tcttcagaca tctcacaaac cctctgcaga ggatgcagag ggccagagtc ccctttctca
     4501 gaagtacagc ccttccacag agaaatgcct gcctgagatt caggggatct ttgacaggga
     4561 tccagacaca ctactttatt tacttcagca aaagagtgag ccagcagagc catgtattgg
     4621 aagcaaagcc ccaaaagatg ataaaacaat tatagaggag caggcaacca aaattgcaga
     4681 tttgaagagg catgtggaat tccttgtggc tgagaatgaa agattaagga aagaaaataa
     4741 acaactaaag gctgaaaagg ccagacttct aaaaggtcca atagaaaagg agctggatgt
     4801 agatgctgat tttgtagaaa cgtcagagtt atggagcttg ccaccacatg cagaaactgc
     4861 tacagcctcc tcaacctggc agaagttcgc agcaaatact gggaaagcca aggacattcc
     4921 aatccccaat cttcctccct tggattttcc atctccagaa cttcctctta tggagctctc
     4981 tgaggatatt ctgaaaggat ttatgaataa ttaagtcgag tctagagggc ccgtttaaac
     5041 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5101 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5161 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5221 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5281 ggcttctgag gcggaaagaa ccagctgggg ctctaggcgc gttgacattg attattgact
     5341 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     5401 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     5461 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5521 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     5581 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     5641 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     5701 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     5761 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     5821 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     5881 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     5941 cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg agctcggatc
     6001 cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac tggcggccgc
     6061 tcgagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     6121 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     6181 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     6241 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     6301 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     6361 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     6421 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     6481 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     6541 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     6601 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     6661 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     6721 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     6781 agtaagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact
     6841 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6901 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6961 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7021 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7081 accagctggg gctctaggcg cgttgacatt gattattgac tagttattaa tagtaatcaa
     7141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     7201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     7261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     7321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     7381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     7441 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     7501 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     7561 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     7621 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     7681 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
     7741 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     7801 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacca
     7861 tggaagggtc taagacgtcc aacaacagca ccatgcaggt gagcttcgtg tgccagcgct
     7921 gcagccagcc cctgaaactg gacacgagtt tcaagatcct ggaccgtgtc accatccagg
     7981 aactcacagc tccattactt accacagccc aggcgaaacc aggagagacc caggaggaag
     8041 agactaactc aggagaggag ccatttattg aaactcctcg ccaggatggt gtctctcgca
     8101 gattcatccc cccagccagg atgatgtcca cagaaagtgc caacagcttc actctgattg
     8161 gggaggcatc tgatggcggc accatggaga acctcagccg aagactgaag gtcactgggg
     8221 acctttttga catcatgtcg ggccagacag atgtggatca cccactctgt gaggaatgca
     8281 cagatactct tttagaccag ctggacactc agctcaacgt cactgaaaat gagtgtcaga
     8341 actacaaacg ctgtttggag atcttagagc aaatgaatga ggatgacagt gaacagttac
     8401 agatggagct aaaggagctg gcactagagg aggagaggct gatccaggag ctggaagacg
     8461 tggaaaagaa ccgcaagata gtggcagaaa atctcgagaa ggtccaggct gaggctgaga
     8521 gactggatca ggaggaagct cagtatcaga gagaatacag tgaatttaaa cgacagcagc
     8581 tggagctgga tgatgagctg aagagtgttg aaaaccagat gcgttatgcc cagacgcagc
     8641 tggataagct gaagaaaacc aacgtcttta atgcaacctt ccacatctgg cacagtggac
     8701 agtttggcac aatcaataac ttcaggctgg gtcgcctgcc cagtgttccc gtggaatgga
     8761 atgagattaa tgctgcttgg ggccagactg tgttgctgct ccatgctctg gccaataaga
     8821 tgggtctgaa atttcagaga taccgacttg ttccttacgg aaaccattca tatctggagt
     8881 ctctgacaga caaatctaag gagctgccgt tatactgttc tggggggttg cggtttttct
     8941 gggacaacaa gtttgaccat gcaatggtgg ctttcctgga ctgtgtgcag cagttcaaag
     9001 aagaggttga gaaaggcgag acacgttttt gtcttcccta caggatggat gtggagaaag
     9061 gcaagattga agacacagga ggcagtggcg gctcctattc catcaaaacc cagtttaact
     9121 ctgaggagca gtggacaaaa gctctcaagt tcatgctgac gaatctgaag tggggtctgg
     9181 cttgggtgtc ctctcaattt tataacaaat gagtcgagtc tagagggccc gtttaaaccc
     9241 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     9301 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     9361 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     9421 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     9481 cttctgaggc ggaaagaacc agctggggct ctaggcgcgt tgacattgat tattgactag
     9541 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     9601 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     9661 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     9721 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     9781 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     9841 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     9901 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     9961 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
    10021 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
    10081 gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga
    10141 tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
    10201 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa
    10261 acttaagctt ggtaccatgg gggaagcaga gaagtttcac tacatctata gttgtgacct
    10321 ggatatcaac gtccagctta agataggaag cttggaaggg aagagagaac aaaagagtta
    10381 taaagctgtc ctggaagacc caatgttgaa gttctcagga ctatatcaag agacatgctc
    10441 tgatctttat gttacttgtc aagtttttgc agaagggaag cctttggcct tgccagtgag
    10501 aacatcctac aaagcattta gtacaagatg gaactggaat gaatggctga aactaccagt
    10561 aaaataccct gacctgccca ggaatgccca agtggccctc accatctggg atgtgtatgg
    10621 tcccggaaaa gcagtgcctg taggaggaac aacggtttcg ctctttggaa aatacggcat
    10681 gtttcgccaa gggatgcatg acttgaaagt ctggcctaat gtagaagcag atggatcaga
    10741 acccacaaaa actcctggca gaacaagtag cactctctca gaagatcaga tgagccgtct
    10801 tgccaagctc accaaagctc atcgacaagg acacatggtg aaagtagatt ggctggatag
    10861 attgacattt agagaaatag aaatgataaa tgagagtgaa aaacgaagtt ctaatttcat
    10921 gtacctgatg gttgaatttc gatgtgtcaa gtgtgatgat aaggaatatg gtattgttta
    10981 ttatgaaaag gacggtgatg aatcatctcc aattttaaca agttttgaat tagtgaaagt
    11041 tcctgacccc cagatgtcta tggagaattt agttgagagc aaacaccaca agcttgcccg
    11101 gagtttaaga agtggacctt ctgaccacga tctgaaaccc aatgctgcca cgagagatca
    11161 gttaaatatt attgtgagtt atccaccaac caagcaactt acctatgaag aacaagatct
    11221 tgtttggaag tttagatatt atcttacgaa tcaagaaaaa gccttgacaa aattcttgaa
    11281 atgtgttaat tgggatctac ctcaagaggc caaacaggcc ttggaacttc tgggaaaatg
    11341 gaagccgatg gatgtagagg actccttgga gctgttatcc tctcattaca ccaacccaac
    11401 tgtgaggcgt tatgctgttg cccggttgcg acaggccgat gatgaggatt tgttgatgta
    11461 cctattacaa ttggtccagg ctctcaaata tgaaaatttt gatgatataa agaatggatt
    11521 ggaacctacc aagaaggata gtcagagttc agtgtcagaa aatgtgtcaa attctggaat
    11581 aaattctgca gaaatagata gctcccaaat tataaccagc ccccttcctt cagtctcttc
    11641 acctcctcct gcatcaaaaa caaaagaagt tccagatggc gaaaatctgg aacaagatct
    11701 ctgtaccttc ttgatatcga gagcctgcaa aaactcaaca ctggctaatt atttatactg
    11761 gtatgtgata gtggaatgtg aagatcaaga tactcagcag agagatccaa agacccatga
    11821 gatgtacttg aacgtaatga gaagattcag ccaagcattg ttgaagggtg ataagtctgt
    11881 cagagttatg cgttctttgc tggctgcaca acagacattt gtagatcggt tggtgcatct
    11941 aatgaaggca gtacaacgcg aaagtggaaa tcgtaagaaa aagaatgaga gactacaggc
    12001 attgcttgga gataatgaaa agatgaattt gtcagatgtg gaacttatcc cgttgccttt
    12061 agaaccccaa gtgaaaatta gaggaataat tccggaaaca gctacactgt ttaaaagtgc
    12121 ccttatgcct gcacagttgt tttttaagac ggaagatgga ggcaaatatc cagttatatt
    12181 taagcatgga gatgatttac gtcaagatca acttattctt caaatcattt cactcatgga
    12241 caagctgtta cggaaagaaa atctggactt gaaattgaca ccttataagg tgttagccac
    12301 cagtacaaaa catggcttca tgcagtttat ccagtcagtt cctgtggctg aagttcttga
    12361 tacagaggga agcattcaga acttttttag aaaatatgca ccaagtgaga atgggccaaa
    12421 tgggattagt gctgaggtca tggacactta cgttaaaagc tgtgctggat attgcgtgat
    12481 cacctatata cttggagttg gagacaggca cctggataac cttttgctaa caaaaacagg
    12541 caaactcttc cacatagact ttggatatat tctgggtcgt gatccaaagc ctcttcctcc
    12601 accaatgaag ctgaataaag aaatggtaga aggaatgggg ggcacacaga gtgagcagta
    12661 ccaagagttc cgtaaacagt gttacacggc tttcctccac ctgcgaaggt attctaatct
    12721 gattttgaac ttgttttcct tgatggttga tgcaaacatt ccagatattg cacttgaacc
    12781 agataaaact gtgaaaaagg ttcaggataa attccgctta gacctgtcgg atgaagaggc
    12841 tgtgcattac atgcagagtc tgattgatga gagtgtccat gctctttttg ctgcagtggt
    12901 ggaacagatt cacaagtttg cccagtactg gcgcaaatga gtcgagtcta gagggcccgt
    12961 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    13021 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    13081 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    13141 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    13201 ctctatggct tctgaggcgg aaagaaccag ctggggctct aggcgcgttg acattgatta
    13261 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    13321 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    13381 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    13441 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    13501 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    13561 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    13621 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    13681 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    13741 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    13801 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
    13861 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
    13921 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
    13981 gcgtttaaac ttaagcttat gggaaatcag cttgctggca ttgctccctc ccagatcctt
    14041 tctgtagaga gttatttttc agatattcat gactttgaat atgataaaag cctggggagt
    14101 actcggtttt ttaaagttgc tcgagccaag caccgagaag gcctggtcgt tgtgaaggtt
    14161 tttgcaattc aggatcccac attgccttta accagctata aacaagagct ggaggaactg
    14221 aaaatcaggc ttaattctgc acagaattgt ctacctttcc agaaagcatc agaaaaagca
    14281 tctgagaaag cagctatgct ctttaggcag tatgtgcgag acaatctcta tgatcgcatc
    14341 agtacccgtc cattcttgaa taacattgag aagcgctgga ttgctttcca gatcctgaca
    14401 gctgtggacc aagcacacaa atctggagtt cgtcatgggg acatcaagac tgagaatgtg
    14461 atggtcacca gttggaattg ggttcttcta actgattttg ccagttttaa gcccacttat
    14521 cttccagaag acaacccggc agatttcaat tatttctttg acacatcacg gaggagaact
    14581 tgctatattg ctcctgaacg ttttgttgat ggtgggatgt ttgccactga gttagaatat
    14641 atgagagatc cttcaactcc gcttgtagac ttaaatagca atcagagaac aagaggagag
    14701 ttgaagagag caatggacat cttttcagca ggttgtgtga tagctgagct ttttacagaa
    14761 ggtgtaccat tatttgatct ctctcaactt ttggcttata gaaatggaca ttttttccct
    14821 gaacaagtgc taaataaaat tgaagatcac agtatcagag aattggtaac tcagatgatt
    14881 caccgtgagc cagataaacg tttagaggca gaagattact taaaacagca gcgtggcaat
    14941 gcctttcctg aaatatttta cacttttctt cagccctaca tggcccagtt tgccaaggaa
    15001 acgtttcttt ctgcagatga gcgtattctg gttatacgga aggatttggg caacattatt
    15061 cacaatctct gtggacatga tctgccagaa aaagccgaag gagagcctaa ggaaaatggg
    15121 ctggttatct tggtatctgt tataacatcc tgcctacaga cccttaaata ctgtgattcc
    15181 aaactagctg ctttggaact gattcttcat ttggctccaa gattaagtgt tgaaatcctt
    15241 ttggatcgta ttactccata tcttttgcat ttcagcaatg actctgttcc tagggtgagg
    15301 gctgaagcct tgaggacgtt gaccaaagtt cttgctctcg tcaaagaggt tcctcgtaat
    15361 gatatcaata tttatccgga atacattctg ccaggcatag cccacttagc ccaagatgat
    15421 gctactatcg ttagactagc ctatgctgaa aacatagctc tgctggcaga aacagctctg
    15481 agattcctgg aattagtaca gttaaaaaat cttaatatgg aaaatgaccc caataatgaa
    15541 gaaatagatg aggttacaca tccaaatgga aattatgaca cagagctgca agccttacat
    15601 gaaatggtcc agcagaaagt tgttactttg ctaagtgacc ctgaaaatat tgtaaaacaa
    15661 accttgatgg aaaatggaat aacacggctg tgtgtattct ttggacgtca gaaagccaac
    15721 gatgttttgt tgtcccacat gattactttc ctaaatgata agaatgattg gcatctacgt
    15781 ggagcatttt ttgatagtat agttggtgtt gctgcctatg ttggctggca aagctcctca
    15841 attctcaagc ctctgctgca acaaggtctt agtgatgctg aggaatttgt cattgtgaaa
    15901 gctctttatg cccttacttg tatgtgccag ttaggactgc tacaaaaacc ccatgtttac
    15961 gaatttgcca gtgatattgc ccccttcctg tgtcatccca atttatggat acgttatggt
    16021 gccgtgggat ttatcacagt ggtagctcgt caaataagta cagctgatgt ctactgtaaa
    16081 ctgatgcctt atcttgaccc atatattacc caaccaataa tacagattga aagaaaactt
    16141 gttctgctca gtgttttaaa ggaaccagta agtcgttcta tatttgatta tgctttgagg
    16201 tctaaagata ttactagctt gttcagacat cttcacatgc gtcagaagaa acgaaatggt
    16261 tctcttcccg actgccctcc gccagaggat cctgccatag cacagcttct gaagaagttg
    16321 ctctcacagg gaatgacaga ggaagaggaa gacaaacttc tggcactgaa agacttcatg
    16381 atgaaatcta ataaagcaaa ggccaatata gtggaccaga gccatcttca tgatagtagt
    16441 cagaaaggtg taattgactt ggcagcttta ggcataactg ggagacaagt tgatcttgtt
    16501 aaaaccaaac aagaaccaga tgacaaacgg gccagaaaac atgtaaaaca agactcaaat
    16561 gtaaatgaag aatggaaaag catgtttggg tcactggacc caccaaacat gccacaggcc
    16621 ctacctaaag ggagtgatca ggaggtgatt cagactggga aacctcctcg ttccgagtcc
    16681 tctgctggca tttgtgtccc tttgtcaact tcttcacagg ttccagaagt gacaactgtc
    16741 caaaataaaa aaccagtaat accggtttta agtagtacaa tcttaccatc cacctatcag
    16801 attcgaatta caacttgtaa aactgaactt cagcaactca tccagcaaaa gcgggagcag
    16861 tgcaatgctg agagaatagc taagcagatg atggaaaatg ctgaatggga gagtaaacca
    16921 ccaccacctg gatggcgtcc taaagggctg ttagttgccc atcttcatga gcataaatct
    16981 gctgtgaatc gaattagagt ctctgatgaa cactcacttt ttgcaacatg ttcaaatgat
    17041 ggcacagtga aaatctggaa cagtcaaaag atggagggga agaccaccac taccagatct
    17101 attcttacat acagccgaat tggaggacga gtcaagacgc tcacattctg ccaaggctcc
    17161 cactatttag ccatagcatc tgataatggt gctgtccagc ttcttggaat tgaggcttct
    17221 aagctgccca agtctcctaa aatccatcct ctacaaagca gaattctaga tcagaaggag
    17281 gacggttgtg ttgtggatat gcatcacttc aactctggag cacagtctgt tcttgcctat
    17341 gccactgtga atggctctct ggttggctgg gaccttaggt cttcaagcaa tgcgtggact
    17401 ttaaagcatg atttaaagtc gggcctcatc acttcctttg ctgtggacat ccaccaatgc
    17461 tggctctgca ttggtacaag cagtggcacc atggcttgtt gggacatgag gttccagttg
    17521 ccaatttcaa gtcactgtca tccttccagg gctcgaatca gacgcctctc aatgcaccct
    17581 ctgtatcagt cctgggtgat tgcagctgtt cagggcaaca acgaagtgtc catgtgggac
    17641 atggagactg gtgacagaag atttactctc tgggccagca gtgcaccacc actttctgaa
    17701 ttacagcctt ctcctcatag cgtccatggt atctactgta gtcctgcaga tggaaatcct
    17761 atcctactaa cagctggctc agatatgaaa ataaggtttt gggacttggc ttacccagaa
    17821 aggtcctatg ttgttgcagg aagtactagt tccccatctg tgtcctacta caggaaaata
    17881 attgaaggca ctgaagttgt ccaggaaatt cagaataagc agaaagtagg accaagtgat
    17941 gacacccctc gaaggggccc agagtccctg cccgtgggac atcatgacat catcactgat
    18001 gtcgccacat tccagaccac acagggcttc atcgtaactg cttctagaga tgggattgtg
    18061 aaggtgtgga aataagtcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
    18121 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    18181 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    18241 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    18301 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
    18361 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
    18421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    18481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    18541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    18601 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    18661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    18721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    18781 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
    18841 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    18901 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    18961 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    19021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    19081 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    19141 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    19201 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
    19261 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
    19321 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
    19381 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
    19441 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
    19501 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
    19561 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
    19621 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    19681 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    19741 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    19801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    19861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    19921 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    19981 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    20041 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    20101 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    20161 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    20221 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    20281 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    20341 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    20401 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    20461 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    20521 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    20581 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    20641 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    20701 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    20761 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    20821 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    20881 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    20941 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    21001 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    21061 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    21121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    21181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    21241 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    21301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    21361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    21421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    21481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    21541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    21601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    21661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    21721 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    21781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    21841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    21901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    21961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    22021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    22081 tccccgaaaa gtgccacctg a
//
