LOCUS       sequence-494292-       13437 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate 
            inducible promoter, without accessory genes (inactive), in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN7-Pct3-(–)-Eldh2(cHis6)-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..13437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             5491..5508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7061..7088
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7220..7247
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7679..8530)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8517..9356)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(9867..10838)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12077..12164
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      12506..12900
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13063..13106)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13202..13311
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaatggg taaactgcat atgtcgcgac gggatttcgt caaagtctcg
     2641 gtcgtgacag gcgcggcgtt cggcatctcc gcgttggctg agccgagttt ggctgttgct
     2701 gacgatgccg tcgataagcc ggtgggaggt gacatcaagc gtatccgaac agggtgtcgc
     2761 ggctgcggca aaatggaatg cggcgttatc gccgtagtgc agaacgggcg tgtcgttcgg
     2821 atggagggcg accctacggc gttccagtcg atggggaact gctgcaccaa atcgcaagcg
     2881 gccattcagg cggcatacca tcctgatcgt ctgcattatc ctatgaaacg aacgaacgac
     2941 aaggaatccg atgatcccgg ttgggtgcgt atttcttggg atgaggccat gaccacgatc
     3001 gtgtcgaaat tcgacgagct gcaagcgcgc tacggaggcg aatcgctgtt cggcatgtgc
     3061 ggcacgtcgc gcgtgtggtg catgttcgga gcgtccaacg gcatgtacct gtgggattcg
     3121 ccgaacatcg tgcaggcgtg gcagatctgc aagggcccgc gccatttcgc cactcttatg
     3181 tgctcgagct tcgccgacag ctggatggag acggttgccc atcctgatgt gtacgtggcc
     3241 tggggcggtg catccgagat gtcgaactac gatgacagct gccgcaccac tgtggatgtg
     3301 gcgacgcgct cgaccacgca catttgcgtc gatccgcgcg aaacgaacat gggtaaagag
     3361 gcggacttcc agctgcacct gcgacccggt accgatgggg cgatggcgtt ggcttggacg
     3421 aacgtcatca ttgaaaacaa tctgtacgac gagttgttca cgaagaagtg gaccaacggt
     3481 ccgtttctcg tgtgcgaaga catggagccg accggctgga ccgaggcttc gcccattgtc
     3541 atgaccccat tcgaactcaa gacgcatttg ctgaaggaat cggatctgct ggaggacgga
     3601 agcccaaagc gttttatggt ttgggacaac cttgctggga ggctgaccta tttcgattcc
     3661 gaaaccggtt tttgggaagg tgaggaatgg acgaagccga cggccgggaa ggaagcttcg
     3721 caggagaatc tcgcgcctgg cttccatcaa ggttttgtca ttgatcctac tccgttcgac
     3781 cctgaaatag atccggcgtt gtttggggaa ttcgaagtga cgctcaaaga cgggtcgacg
     3841 cataaggtgc agccggtgtt tcaaaagtac gccgatcgct gcgcggaata cactcctgaa
     3901 gtcgctgagg aaatcaccgg ggttccggcc gatcagattc gtgcggcggc tatggcttac
     3961 ggcacgcccc ttcatccgga aaagggttat ggcaatggcg gtattcagta catgctggcc
     4021 accgagcatg cgaataccgc catccagaat gtgcgcgcgc ttgattatct gacggccatc
     4081 acgggaaatt acgatacgcc gggcggtcag cgtgcgtcaa cgcgcgctcc catcgagggc
     4141 ggtcagatgg gttttgccaa caacggttcg ggcatgccca tgctttcgcc ggggcagatg
     4201 gagaagatct taggtcgcga tgagattccg ctgctgtatt ggtggggcat gtgggccgat
     4261 gctgcggcta cgtggaatgc ggtgctcacc ggagaaccgt atccggtcgt tggcgcgttc
     4321 aattcgtcgg ggaacttcat gaaccagtgc aacaccacca cgacatggga ggctctcaag
     4381 aagctcgatt tttatgtgga atgcaacctg tggcacactc ctggcggtgg tttgtgcgac
     4441 atcgttttgc ctgccgctca tttcttggag ttgtccagtc cgcgttcatc ccagggcgct
     4501 tcgggcgcta tgtgcgcgac ggtcaaatgc atcgacccgc ctggtgaagc gaagttcgac
     4561 ggcgagatca ttcgcatgtt gtacgaatac aaaggcctcc cctataacgt tattcctggt
     4621 tatcctgaat atcctagcgt cgaggaaatg ctggacatgt cggtggcggg tttcgagccg
     4681 aagggatgga aggtattcga gcagcgtttc caggaacatg gctggtggga ttgcaaggaa
     4741 actgaaccgg aattgtgggg aacgtatcgc cgctacgaaa ccggtgcgaa tcgcgctcgc
     4801 aatgcgggtg ggttcgtagg ttctcaaggt gattggcttc ccgggttcta cactccgaca
     4861 aagaagatcg aaatatggtc tacggtcatg gagtcgttcc atcccgatgg cgagtggaat
     4921 cttcccacgt atcgcgaacc accgcacagt cctgtgagcg atcctgacat gtacaaagaa
     4981 tacccgctga tcatcactac gggacgccgc attcccgtgt acttccattc cgagcaccgg
     5041 cagctgccgt ggtgtcgcga gcaatggccg gtgccgcgcg tggaaatcaa tccagacgat
     5101 gccgccacgt atggcataga acagggagat tgggtttgga ttgagacgcc atatgggaag
     5161 attcgcgagg ttgccgattt gtactacggg atccgtcccg gcactatcaa ttgcgagcat
     5221 cagtggtggt tcccggaatt caagcaatcg ggatcgggcc acgagctatg cgcggtcaac
     5281 cacttgatag acaactgggc gcgcgatccg cattgcggcg cgtctaacct gcgtgcgtac
     5341 ctcggcaaga tatacaaggc aacgcccgag aactccccgt tcggcaatcc ggtgccttgc
     5401 gaccacaacg gcgttgagat catccacgat gcttccgatc ctcgtctcaa agaatggctg
     5461 ccgacgtacg aagggaggga gggcagcagc catcaccatc accatcacta ggccatgacc
     5521 cagtatgcta ttgtcaccga cttgaatcgg tgcgtcgggt gcttggcatg cgctgtcgca
     5581 tgcaaagccg tgaacgatgt gcccattggc agtttttgga acaaggtttt acgcatcggg
     5641 ccgaatgctt ctatacaagg tgcgcaggat gcggattctt atctttactt cctgcccgtg
     5701 cagtgccagc actgcgccga ccccgagtgc gtgaaggtgt gcccgacggg cgcctcgcac
     5761 aagcttgagg acggcacggt gcaggttgac aagtccaagt gcatcggctg ccagttctgc
     5821 gcgatggctt gtccctacaa cgttcgttac ttgaacgagc aggagcgcgt ggttgagaag
     5881 tgcacccttt gcgagcagaa gatcgcccag ggcgagctgc cgcaatgcgt cagccaatgc
     5941 ggtggcatgg cgcgcttctt cggtgatttg gataagggca tcgaatcgtt cgaaggtgcg
     6001 gaaaacggaa ccgagcgcat tgttctcggg gagttctgca agcccttcac ggatgaagac
     6061 gtgcacgtgc tgcctaatgt gggaaacaat ccgtcggtgc gctatatcct gcgtgaccat
     6121 gaatggcaag gaggcggtga ataatggagc tccagtggcc tttgatcgtg ttcaccacgc
     6181 tggtggcgtg gagcgcgggg ctgttcggga cgcaggcgct gatggccgcg ttcggcgtcg
     6241 ggaagaaggc gcaggtcccg gcctggatcg cgagcgccgt ccttctggcc gtcggcggga
     6301 tcgcggtgtt cttccacctc gagcactggg agcgcatctt caacggcttc ggccacctga
     6361 cgagcggcat cacccaggag ctgatcgcca tcgtcgtgct ggcggccgtc gccgtggcct
     6421 acctggtcat gctgcgcaag tccgacgacg gcgcgagcgt gccgaagtgg ctggcgtggc
     6481 tgtcggtcgc gctgtcggtc gcgctggtgg ccgtgatggc gcactcgtac acgatggccg
     6541 cgaggccggc gtgggatagc gtgctgtgga tcctctacgt cctgggcaac gcctgcgtgc
     6601 tgggaccggc gacgttcgcg ttcctcatgg ccgcagcgcc gcccgcaggt cagcccgccg
     6661 ggcgcgcggc cgacgccggg gctcccgcag gcctcgctgc cctcgtaggc gccgccgcga
     6721 acgccctggc cgccctcgcc ttcgcgatct tcctccagct ctccgccggc tccttcgccg
     6781 acgtcggcta ctacttcgac ccgacgcatc cgaccgaggc catggccgac gccgcggcga
     6841 cggtcggcgc gcaagcgccc ctgctgtggc tcggcgcggt cgccgtcggc gctatcgtcc
     6901 cgctcgcggc ggccttcatg ggcaggcgct ccggcaactg gaggctctgg gccccggtgg
     6961 ctatcatcgc cgccctggcg ggcgccgtct gcatgcgcgt ggccttctac aacctcggcc
     7021 tcagcgtgtt catgttctac tagactagtc gactcacact ggctcacctt cgggtgggcc
     7081 tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat ccggcttttt
     7141 tattatttaa ttcctgttat aaaaaaagga tcaattttga actctctccc aaagttgatc
     7201 ccttaacgat ccctgcagga gaaggccatc ctgacggatg gcctttttga agctttctag
     7261 aatcgggggc ccccgggggg attctagaac aatggaagtc ctcgtctcgc cctcgaatta
     7321 gcccgcttaa tgagcgggct ttttttgaat taattctcgc gagctggcac gacaggtttc
     7381 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagcgc gaattgcaag
     7441 ctggccgacg cgctgggcta cgtcttgctg gcgttcggga gcagaagagc atacatctgg
     7501 aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc
     7561 aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga
     7621 aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt ggggggggtc
     7681 agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc
     7741 agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg
     7801 tcgaaccact ggcctctgac ggccagacat agccgcacaa ggtatctatg gaagccttgc
     7861 cggttttgcc ggggtcgatc cagccacaca gccgctggtg cagcaggcgg gcggtttcgc
     7921 tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg ctggccgcca cccatgacgg
     7981 cctgcgcgat caaggggttc agggccacgt acaggcgccc gtccgcctcg tcgctggcgt
     8041 actccgacag cagccgaaac ccctgccgct tgcggccatt ctgggcgatg atggatacct
     8101 tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta tcgacctctg
     8161 ccccgatttc ctttgccagc gcccgatagc tacctttgac caccatggca tcagcggtga
     8221 cggcctccca cttgggttcc aggaacagcc ggagctgccg tccgccttcg gtcttgggtt
     8281 ccgggccaag cactaggcca ttaggcccag ccatggccac cagcccttgc aggatgcgca
     8341 gatcatcagc gcccagcggc tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt
     8401 catacgtcac gtccagcttg ctgcgcttgc gctcgccccg cttgagggca cggaacaggc
     8461 cgggggccag acagtgcgcc gggtcgtgtc ggacgtggct gaggctgtgc ttgttcttag
     8521 gcttcaccac ggggcacccc cttgctcttg cgctgcctct ccagcacggc gggcttgagc
     8581 accccgccgt catgccgcct gaaccaccga tcagcgaacg gtgcgccata gttggccttg
     8641 ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc
     8701 tcggcgctgg tcatgctcga caggtaggac tgccagcgga tgttatcgac cagtaccgag
     8761 ctgccccggc tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc cttgctggca
     8821 tggtgcagga acacgataga gcacccggta tcggcggcga tggcctccat gcgaccgatg
     8881 acctgggcca tggggccgct ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc
     8941 agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc
     9001 atgatgttgg gcaggctgcc gatcagcggc tggatcagca ggccgtcagc cacggcttgc
     9061 cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc
     9121 gggtcttcgg cgggcaggta gatcaccggg ccggtgggca gttcgcccac ctccagcaga
     9181 tccggcccgc ctgcaatctg tgcggccagt tgcagggcca gcatggattt accggcacca
     9241 ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac gtagtccagc
     9301 ggtggcggcg ctgctgcgaa cgcctccaga atattgatag gcttatgggt agccattgat
     9361 tgcctccttt gcaggcagtt ggtggttagg cgctggcggg gtcactaccc ccgccctgcg
     9421 ccgctctgag ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg gtcagccaga
     9481 acttgcgctg acgcatccct ttggccttca tgcgctcggc atatcgcgct tggcgtacag
     9541 cgtcagggct ggccagcagg tcgccggtct gcttgtcctt ttggtctttc atatcagtca
     9601 ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa ggtgcagccg
     9661 tcaaggttaa ggctggccat atcagcgact gaaaagcggc cagcctcggc cttgtttgac
     9721 gtataaccaa agccaccggg caaccaatag cccttgtcac ttttgatcag gtagaccgac
     9781 cctgaagcgc ttttttcgta ttccataaaa cccccttctg tgcgtgagta ctcatagtat
     9841 aacaggcgtg agtaccaacg caagcactac atgctgaaat ctggcccgcc cctgtccatg
     9901 cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg gacgctgggc
     9961 agacccatga ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc gggcttgcgc
    10021 tctgccagcg ctgggctggc ctcggccatg gccttgccga tttcctcggc actgcggccc
    10081 cggctggcca gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc accgaagcgc
    10141 ttgaccagcc cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg gtggcggcta
    10201 agctgccgct cggacagttc gaggctggcc agcctgcggg ccttctcctg ctgccgctgg
    10261 gcctgctcga tctgctggcc agcctgctgc accagcgccg ggccagcggt ggcggtcttg
    10321 cccttggatt cacgcagcag cacccacggc tgataaccgg cgcgggtggt gtgcttgtcc
    10381 ttgcggttgg tgaagcccgc caagcggcca tagtggcggc tgtcggcgct ggccgggtcg
    10441 gcgtcgtact cgctggccag cgtccgggca atctgccccc gaagttcacc gcctgcggcg
    10501 tcggccacct tgacccatgc ctgatagttc ttcgggctgg tttccactac cagggcaggc
    10561 tcccggccct cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc caccagcacc
    10621 agaccatgcc gctcctgctc ggcgggcctg atatacacgt cattgccctg ggcattcatc
    10681 cgcttgagcc atggcgtgtt ctggagcact tcggcggctg accattcccg gttcatcatc
    10741 tggccggtgg tggcgtccct gacgccgata tcgaagcgct cacagcccat ggccttgagc
    10801 tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag cgcagccaga
    10861 tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat gcgatcagca
    10921 gcaccaccgt aggcatcatg gaagccagca tcacggttag ccatagcttc cagtgccacc
    10981 cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct acctcccgca
    11041 actctttggc cagctccacc catgccgccc ctgtctggcg ctgggctttc agccactccg
    11101 ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac ctgccgggct tcgtcggcca
    11161 gtgtcgccat gctctgggcc agcggttcga tctgctccgc taactcgttg atgcctctgg
    11221 atttcttcac tctgtcgatt gcgttcatgg tctattgcct cccggtattc ctgtaagtcg
    11281 atgatctggg cgttggcggt gtcgatgttc agggccacgt ctgcccggtc ggtgcggatg
    11341 ccccggcctt ccatctccac cacgttcggc cccaggtgaa caccgggcag gcgctcgatg
    11401 ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt ggcctgcccg ctctaatgcc
    11461 cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt gggcttgagc
    11521 gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg cttgaaccac
    11581 tgagcggcgg gccgctcgat gccgtcattg atccgctcgg agatcatcag gtggcagtgc
    11641 gggttctcgc cgccaccggc atggatggcc agcgtatacg gcaggcgctc ggcaccggtc
    11701 aggtgctggg cgaactcgga cgccagcgcc ttctgctggt cgagggtcag ctcgaccggc
    11761 agggcaaatt cgacctcctt gaacagccgc ccattggcgc gttcatacag gtcggcagca
    11821 tcccagtagt cggcgggccg ctcgacgaac tccggcatgt gcccggattc ggcgtgcaag
    11881 acttcatcca tgtcgcgggc atacttgcct tcgcgctgga tgaagtcggc cttggccctg
    11941 gccgattggc cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat cgccatgctg
    12001 cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc ctcggagagc gcaccgcccg
    12061 aagggtggcc gttaggccag tttctcgaag agaaaccggt aagtgcgccc tcccctacaa
    12121 agtagggtcg ggattgccgc cgctgtgcct ccatgatagc ctacgagaca gcacattaac
    12181 aatggggtgt caagatggtt aaggggagca acaaggcggc ggatcggctg gccaagctcg
    12241 aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg gaacagcagc
    12301 aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt ttggccaagg
    12361 tgaacagcag cgagtggccg gaggatcggc tcatggcggc aatggatgcg taccttgaac
    12421 gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag ccgggctgaa
    12481 tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc gggtaggggg
    12541 aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt
    12601 ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag
    12661 cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag ggcagcagcg
    12721 ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc atggatggat
    12781 ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg ggcgtgacag
    12841 ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg caggggttcg
    12901 tgacagttag tacgggagtg acgggcactg gctggcaatg tctagcaacg gcaggcattt
    12961 cggctgaggg taaaagaact ttccgctaag cgatagactg tatgtaaaca cagtattgca
    13021 aggacgcgga acatgcctca tgtggcggcc ctcgcgagaa ttaattcaga taaaaaaaat
    13081 ccttagcttt cgctaaggat gatttctagc gatgaccctg ctgattggtt cgctgaccgt
    13141 agactggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
    13201 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
    13261 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
    13321 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
    13381 acatcgcgcg cgcgcgcgcg gatgctagcg gcgcgccaaa aaaaagcccg ctcatta
//
