LOCUS       sequence-494293-       11807 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  Expresses His-tagged Gordonibacter sp. 28C Gs Vadh with a cumate 
            inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for 
            protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 11807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF24-Pct3-GsVadh(nHis6)(Gs28C)
FEATURES             Location/Qualifiers
     source          1..11807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             2600..2617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5431..5458
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5590..5617
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(6049..6900)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6887..7726)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8237..9208)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10447..10534
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10876..11270
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(11433..11476)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            11572..11681
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaaatgc atcaccatca ccatcacgac tacgacatcc cgactaccac
     2641 ggagtggaag aggcaagagg gcgaggagac catcttcagg acctgcgcat ggtcgccccc
     2701 ggggtgccac ccggtgggat gcggtttgca ggtgcacgtc aaagacggca aggtaacgaa
     2761 ggttgaaggc gaccccgagc accccatcac caaaggctcg ctgtgcacgc gctgcctagc
     2821 gcttccggag tacatctacc atcccgaccg catcacgcac ccgctgaagc gcgccaagga
     2881 agaccgcggc aaggacaagt gggagcggtg cacgtgggac gaggccaccg acctcatcgc
     2941 caagaacgcc cgcgagatca ccgagaagta cggcgccgag gccatctgcg tgttcggcgg
     3001 aaccggccgc gagggcaaca actggtaccc gcagtgggcg aacctcgtgt tcggcacgcc
     3061 gaactccgtg tacgcccagg ccggctggtc gtgctacggc ccacgtatga cgaacaccgc
     3121 cttcatgatc ggcggcggct atcccgagat cgactacgcc cagaagttcg aggaccgcta
     3181 cgagcacgag ggttgggtcg cccccgagtg catcctcatc tggggcaagg agccgctgcg
     3241 cagcaacccc gacggcctgt acggccacgc cgtcatcgag atggtgcgcc agttcggcac
     3301 gaagctcatc tgcgtcgacc cacgtgtgac ctggctcggc acgcgctccg aggaagtgct
     3361 gcagctgcgc cccggcaccg acacggcgct ggccatggcg ttcatctacg tcatggcgca
     3421 cgacgacctc gtggaccacg acttcatcga caagtggtgc tacggctacg agcagctcgt
     3481 cgagcgtgta caggacatgc cgcccgcgaa agccgccgag atctgcggcg tgcccgaaga
     3541 gcagatctgg cgcgcggcac ggctgtacgg caacgccaag cccagcagcg tggcctgggg
     3601 cctcgccgtg gacgagaacc cgaacggcac ccagctcggc cagtgcctca tcgcgctcat
     3661 ggccatcacc ggcttcatcg acgcgcccgg cggcacgacg ctcggcatgg gcgacgacca
     3721 gggcaacgtc gtgcgcgacg gcggctccat ctccgccgac gagaaggtgg acgacgccac
     3781 cgactccacg ctggagctcg cgttcaagaa cggcatcatg accgaggaga cgtgggcgaa
     3841 gcgcgtgggc atcgacaagt acccggccat gggctccatc ctgtggacgg tcatgcccga
     3901 cgagttcctg aaggcgcttg agaccgagaa gccctacaag atccatatgg cgcacttcat
     3961 ctcgtcgaac cccatcggca ccgccatctc cgccgagccg cagcgctggt acgaggcgct
     4021 caaaaagctc gacttcaact gcgcggccga cctgttcatg aaccccacca ccatggcctg
     4081 ctgcgacgtg ttcctgccca tcgcctccac catcgagcac gacggcatgg tggtcactca
     4141 ttacggcctg aactcgtcgt tctacggcgc gcagaacaag tgcgtgcagg tgggcgaatg
     4201 caagtccgac gtcgaggcca tgatggccgt gggcaagaag atgtaccccg acttctggaa
     4261 ccgtttcgag acccagaccg actacgacaa cttccacgtc atgcgctcgt ggctgcccta
     4321 cgaggagctg cgcgagaagg tcaccgtcat gtccaacgag ccgtactaca agtacgagat
     4381 cggcaagctg cgtcccgacg gacaggtggg cttccccacc agcacgggcc gcgtggagct
     4441 gtactcctac atgtacgagc agttcggcga gaaccccctg ccctactacg aggatcccgc
     4501 ctacggcccc gagcgcacgc ccgagctcat ggagaagtac cccttcatcc tcaccacggg
     4561 cgcccgtcgc caggagttct tccattccga gcacaagcag gttcccagcc tgcgccagat
     4621 caccccgtat ccgcagatcg acctcaaccc cgccgatgcc gcgcgcctcg gcatcgaaga
     4681 ggacgactgg gtggagatcg cctcgcccta cggcgccgtg cggcagcgcg cccacgtggt
     4741 gcccaccatc aaggagggcg tgtgccacgc catgcacggc tggtggtacc ccgaggaaga
     4801 cggcgaggaa cccaacctgt tcggcaactg gaagtcgaac atcaacgtgc tcatgcccaa
     4861 cagcgtgaac ggcgtgatgg gcttcggcaa cacgttcaag cagatgatct gctcgctgaa
     4921 gaaagtcgag ggcgagggag gcccgaacga tcccgacaac accggcatct tcctggagcc
     4981 cagccgccag gccgagttca cctaccagca ggtatggcgt cccaccgacg aaaccaagca
     5041 ggcttagaac cgtttgaaag agaaggaggt actgaaaatg acgcaaatgg ccctgtacat
     5101 cgactacgaa ttctgctctg gttgccacag ctgcgaagtc gcatgccgca acgaaatcga
     5161 gtgcggaatc gacgattggg gaatcaaagt cctcgaggac aagccgcgcc agcacgctga
     5221 cggctcctgg cactgggact acctggcgct tccgggcgag ctgtgcgaca tgtgcgaaga
     5281 gcgcgtcgcg gcgggaaagc ttcccgcctg cgtgcagtcc tgccaggcca agtgccttga
     5341 gatcggaacg atcgaggaat gcgcccaaaa gcttgccgcc aagggcaaga aagcggcgat
     5401 cctcgttcct tagactagtc gactcacact ggctcacctt cgggtgggcc tttctgcgtt
     5461 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattatttaa
     5521 ttcctgttat aaaaaaagga tcaattttga actctctccc aaagttgatc ccttaacgat
     5581 ccctgcagga gaaggccatc ctgacggatg gcctttttga agctttctag aatcgggggc
     5641 ccccgggggg attctagaac aatggaagtc ctcgtctcgc cctcgaatta gcccgcttaa
     5701 tgagcgggct ttttttgaat taattctcgc gagctggcac gacaggtttc ccgactggaa
     5761 agcgggcagt gagcgcaacg caattaatgt aagttagcgc gaattgcaag ctggccgacg
     5821 cgctgggcta cgtcttgctg gcgttcggga gcagaagagc atacatctgg aagcaaagcc
     5881 aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt
     5941 taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca
     6001 ttgaatataa aagataaaaa tgtcttgttt acaatagagt ggggggggtc agcctgccgc
     6061 cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc agcccagcgc
     6121 gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg tcgaaccact
     6181 ggcctctgac ggccagacat agccgcacaa ggtatctatg gaagccttgc cggttttgcc
     6241 ggggtcgatc cagccacaca gccgctggtg cagcaggcgg gcggtttcgc tgtccagcgc
     6301 ccgcacctcg tccatgctga tgcgcacatg ctggccgcca cccatgacgg cctgcgcgat
     6361 caaggggttc agggccacgt acaggcgccc gtccgcctcg tcgctggcgt actccgacag
     6421 cagccgaaac ccctgccgct tgcggccatt ctgggcgatg atggatacct tccaaaggcg
     6481 ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta tcgacctctg ccccgatttc
     6541 ctttgccagc gcccgatagc tacctttgac caccatggca tcagcggtga cggcctccca
     6601 cttgggttcc aggaacagcc ggagctgccg tccgccttcg gtcttgggtt ccgggccaag
     6661 cactaggcca ttaggcccag ccatggccac cagcccttgc aggatgcgca gatcatcagc
     6721 gcccagcggc tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt catacgtcac
     6781 gtccagcttg ctgcgcttgc gctcgccccg cttgagggca cggaacaggc cgggggccag
     6841 acagtgcgcc gggtcgtgtc ggacgtggct gaggctgtgc ttgttcttag gcttcaccac
     6901 ggggcacccc cttgctcttg cgctgcctct ccagcacggc gggcttgagc accccgccgt
     6961 catgccgcct gaaccaccga tcagcgaacg gtgcgccata gttggccttg ctcacaccga
     7021 agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc tcggcgctgg
     7081 tcatgctcga caggtaggac tgccagcgga tgttatcgac cagtaccgag ctgccccggc
     7141 tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc cttgctggca tggtgcagga
     7201 acacgataga gcacccggta tcggcggcga tggcctccat gcgaccgatg acctgggcca
     7261 tggggccgct ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc agcaccatca
     7321 ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc atgatgttgg
     7381 gcaggctgcc gatcagcggc tggatcagca ggccgtcagc cacggcttgc cgttcctcgg
     7441 cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc gggtcttcgg
     7501 cgggcaggta gatcaccggg ccggtgggca gttcgcccac ctccagcaga tccggcccgc
     7561 ctgcaatctg tgcggccagt tgcagggcca gcatggattt accggcacca ccgggcgaca
     7621 ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac gtagtccagc ggtggcggcg
     7681 ctgctgcgaa cgcctccaga atattgatag gcttatgggt agccattgat tgcctccttt
     7741 gcaggcagtt ggtggttagg cgctggcggg gtcactaccc ccgccctgcg ccgctctgag
     7801 ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg gtcagccaga acttgcgctg
     7861 acgcatccct ttggccttca tgcgctcggc atatcgcgct tggcgtacag cgtcagggct
     7921 ggccagcagg tcgccggtct gcttgtcctt ttggtctttc atatcagtca ccgagaaact
     7981 tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa ggtgcagccg tcaaggttaa
     8041 ggctggccat atcagcgact gaaaagcggc cagcctcggc cttgtttgac gtataaccaa
     8101 agccaccggg caaccaatag cccttgtcac ttttgatcag gtagaccgac cctgaagcgc
     8161 ttttttcgta ttccataaaa cccccttctg tgcgtgagta ctcatagtat aacaggcgtg
     8221 agtaccaacg caagcactac atgctgaaat ctggcccgcc cctgtccatg cctcgctggc
     8281 ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg gacgctgggc agacccatga
     8341 ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc gggcttgcgc tctgccagcg
     8401 ctgggctggc ctcggccatg gccttgccga tttcctcggc actgcggccc cggctggcca
     8461 gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc accgaagcgc ttgaccagcc
     8521 cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg gtggcggcta agctgccgct
     8581 cggacagttc gaggctggcc agcctgcggg ccttctcctg ctgccgctgg gcctgctcga
     8641 tctgctggcc agcctgctgc accagcgccg ggccagcggt ggcggtcttg cccttggatt
     8701 cacgcagcag cacccacggc tgataaccgg cgcgggtggt gtgcttgtcc ttgcggttgg
     8761 tgaagcccgc caagcggcca tagtggcggc tgtcggcgct ggccgggtcg gcgtcgtact
     8821 cgctggccag cgtccgggca atctgccccc gaagttcacc gcctgcggcg tcggccacct
     8881 tgacccatgc ctgatagttc ttcgggctgg tttccactac cagggcaggc tcccggccct
     8941 cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc caccagcacc agaccatgcc
     9001 gctcctgctc ggcgggcctg atatacacgt cattgccctg ggcattcatc cgcttgagcc
     9061 atggcgtgtt ctggagcact tcggcggctg accattcccg gttcatcatc tggccggtgg
     9121 tggcgtccct gacgccgata tcgaagcgct cacagcccat ggccttgagc tgtcggccta
     9181 tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag cgcagccaga tcgagccgtc
     9241 ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat gcgatcagca gcaccaccgt
     9301 aggcatcatg gaagccagca tcacggttag ccatagcttc cagtgccacc cccgcgacgc
     9361 gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct acctcccgca actctttggc
     9421 cagctccacc catgccgccc ctgtctggcg ctgggctttc agccactccg ccgcctgcgc
     9481 ctcgctggcc tgctgggtct ggctcatgac ctgccgggct tcgtcggcca gtgtcgccat
     9541 gctctgggcc agcggttcga tctgctccgc taactcgttg atgcctctgg atttcttcac
     9601 tctgtcgatt gcgttcatgg tctattgcct cccggtattc ctgtaagtcg atgatctggg
     9661 cgttggcggt gtcgatgttc agggccacgt ctgcccggtc ggtgcggatg ccccggcctt
     9721 ccatctccac cacgttcggc cccaggtgaa caccgggcag gcgctcgatg ccctgcgcct
     9781 caagtgttct gtggtcaatg cgggcgtcgt ggcctgcccg ctctaatgcc cggttggcat
     9841 ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt gggcttgagc gcttcggtct
     9901 tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg cttgaaccac tgagcggcgg
     9961 gccgctcgat gccgtcattg atccgctcgg agatcatcag gtggcagtgc gggttctcgc
    10021 cgccaccggc atggatggcc agcgtatacg gcaggcgctc ggcaccggtc aggtgctggg
    10081 cgaactcgga cgccagcgcc ttctgctggt cgagggtcag ctcgaccggc agggcaaatt
    10141 cgacctcctt gaacagccgc ccattggcgc gttcatacag gtcggcagca tcccagtagt
    10201 cggcgggccg ctcgacgaac tccggcatgt gcccggattc ggcgtgcaag acttcatcca
    10261 tgtcgcgggc atacttgcct tcgcgctgga tgaagtcggc cttggccctg gccgattggc
    10321 cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat cgccatgctg cctcgctgtt
    10381 gcttttgctt ttcggctcca tgcaatggcc ctcggagagc gcaccgcccg aagggtggcc
    10441 gttaggccag tttctcgaag agaaaccggt aagtgcgccc tcccctacaa agtagggtcg
    10501 ggattgccgc cgctgtgcct ccatgatagc ctacgagaca gcacattaac aatggggtgt
    10561 caagatggtt aaggggagca acaaggcggc ggatcggctg gccaagctcg aagaacaacg
    10621 agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg gaacagcagc aagagcgcaa
    10681 gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt ttggccaagg tgaacagcag
    10741 cgagtggccg gaggatcggc tcatggcggc aatggatgcg taccttgaac gcgaccacga
    10801 ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag ccgggctgaa tgatcgaccg
    10861 agacaggccc tgcggggctg cacacgcgcc cccacccttc gggtaggggg aaaggccgct
    10921 aaagcggcta aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt ttagcgggct
    10981 ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag cgcagcgaat
    11041 agaccagcta tccggcctct ggccgggcat attgggcaag ggcagcagcg ccccacaagg
    11101 gcgctgataa ccgcgcctag tggattattc ttagataatc atggatggat ttttccaaca
    11161 ccccgccagc ccccgcccct gctgggtttg caggtttggg ggcgtgacag ttattgcagg
    11221 ggttcgtgac agttattgca ggggggcgtg acagttattg caggggttcg tgacagttag
    11281 tacgggagtg acgggcactg gctggcaatg tctagcaacg gcaggcattt cggctgaggg
    11341 taaaagaact ttccgctaag cgatagactg tatgtaaaca cagtattgca aggacgcgga
    11401 acatgcctca tgtggcggcc ctcgcgagaa ttaattcaga taaaaaaaat ccttagcttt
    11461 cgctaaggat gatttctagc gatgaccctg ctgattggtt cgctgaccgt agactggttg
    11521 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
    11581 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
    11641 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
    11701 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg acatcgcgcg
    11761 cgcgcgcgcg gatgctagcg gcgcgccaaa aaaaagcccg ctcatta
//
