LOCUS       sequence-494338-       10721 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  Lentiviral vector that expresses wildtype Flag-tagged SMARCAL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Feng S, Liu K, Shang J, Hoeg L, Pastore G, Yang W, Roy S, 
            Sastre-Moreno G, Young JTF, Wu W, Xu D, Durocher D
  TITLE     Profound synthetic lethality between SMARCAL1 and FANCM.
  JOURNAL   Mol Cell. 2024 Dec 5;84(23):4522-4537.e7. doi: 
            10.1016/j.molcel.2024.10.016. Epub 2024 Nov 6.
  PUBMED    39510066
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.10.016"; journalName: "Mol Cell"; date: 
            "2024-12-5- 5"; volume: "84"; issue: "23"; pages: "4522-4537.e7"
COMMENT     Sequence Label: pHIVT9-NAT-3xFlag-SMARCAL1
FEATURES             Location/Qualifiers
     source          1..10721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      143..731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     632..651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             793..973
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(1702..1755)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(2335..2343,2344..2346,2347..2921)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2508..2527)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2737..2754
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5821..5886)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(5898..7076)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5907..6845
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5930..5950)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7139..7256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7554..7598)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7783..8016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8513..8638
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8685..8865
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8883..9081)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8908..8928)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9083..9462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9634..9653
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9728..9832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9833..9901,9902..10693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10051..10070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
       61 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
      121 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
      181 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      241 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      301 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      361 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      421 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      481 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      541 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      601 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      661 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      721 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      781 ttgctcacat gctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
      841 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
      901 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
      961 accagagaga cccagtacag tccggatgca gctctcgggc catgtgatga aatgctaggc
     1021 ggctgtcaaa cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca
     1081 cagggtgtaa caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa
     1141 ctggtactag cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg
     1201 tgtagttctg ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat
     1261 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     1321 taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgactggaa
     1381 aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg gtagctgctg
     1441 tattgctact tgtgattgct ccatgttttt ctaggtctcg atcgaagtcg acggtatcga
     1501 taaatttggc gcgcctcaac tgaagcagaa gaggtggatt atggcattag ccacgaagaa
     1561 aaggaaaatg cctccgctta tgttgctgga tgctgagggg ctgctggttt ggctggtgtc
     1621 gttttgtcct gagagtccag tttgtatctg taccataacc aggaggctga tggtgagtag
     1681 gaggaagagg aagcgcttca ttgggccagg attctcctcg acgtcaccgc atgttagcag
     1741 acttcctctg ccctcgccac tgccggggca gggcatggac atgtagagcg cctgctcgcc
     1801 gtccgaggcg gtgccgtcgt acagggcggt gtccaggccg cagagggtga accccatccg
     1861 ccggtaagcg tggatcgccg gtgcgttgac gttggtgact tcgagccaaa gatgaccagc
     1921 tccccgttct ctggcgaact cggtagccag tcccatcaat gcccgtccca ccccgtgtcc
     1981 cctgtgctcg ggtgctacct caatgtcctc gacggtcagc cggcggttcc agccggagta
     2041 cgagacgacc acgaagcccg ccaggtcgcc gtcgtccccg tacgcgacga acgtccggga
     2101 gtccgggtcg ccgtcctccc cgtcgtccga ttcgtcgtcc gattcgtcgt cggggaacac
     2161 cttggtcagg ggcgggtcca ccggcacctc ccgcagggtg aagccgtccc cggtggcggt
     2221 gacgcggaag acggtgtcgg tggtgaagga cccatccagt gcctcgatgg cctcggcgtc
     2281 cccagggaca ctggtgcggt atcggtaagc cgtgtcgtcc agggtggtgc ccatggttgt
     2341 ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta
     2401 gacgtttttt taacctcgac taaacacatg taaagcatgt gtaccgaggc cccagatcag
     2461 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
     2521 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
     2581 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
     2641 gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca
     2701 gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa
     2761 gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa
     2821 aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc
     2881 ggcttcggcc agtaacgtta gggggggggg agggagaggg gcggatccta gcccgggtta
     2941 caggggagac gtaaagctgt cccagttatc aaaaaattca aatctccttt tcttctgtgg
     3001 actggctgtc aatgagcttt catccagggt gtctcccatg ttctgggaac tacttccaga
     3061 tgttcctgaa gcacttcctg ggtcaaagga ctctgctgct tccaggagct ccatatcact
     3121 tccttctttc tcaaaggact tctggaatag gtcgtagatc ttctgctgct ttgggtcctt
     3181 gtagaggtaa tcagtggatt ctgtcatttc tgaaaaattg gtctcagaaa gcccggcttc
     3241 tgccagaact ttaatcttct cttgaatcag gggccaaagg tagtcatcag ctgtgccctt
     3301 tgccacgagg tagtgaatgc ccacggagct ggtctgtcca atgcggtgca cgcggtcctc
     3361 agcctggatc agcacccctg ggttccaaaa cagctcagca aacaccacca ggtcagccga
     3421 ggagaaggtg aggcccatat tggcagcggt gatggacagc acggccacag catgcctctc
     3481 cgacagttgg aactgctggc acaggtcctc ccgctcagct gatgaggtgg agccatcgat
     3541 gcggatgtgc tgcacgtgct ttctctcaag ctcttgcgta attgcgtcca ggaccacctt
     3601 atggtgtgca aatactaaaa acttctctct tccactttcc agtaggtcca agatatattc
     3661 aatgacagat gggattttag cttcagctgt tctgttgaag aagagaatga gggcatcttt
     3721 ctgctgctgt ttagttttgt ccttggtggt catttccttg gctgcagcat ccagggcagc
     3781 tctggtcctg gcattgatcc gtcctggggc aatcaccact atcttgcgct gcttggcagg
     3841 cagctgggaa aggacgtcgg acttgaggcg ccgcagcatg actgcttcct ccagcaggag
     3901 cttcagctct cccaggttgg aggaacctga gtagtcccac ccccaaggca tccgtttggc
     3961 atcacagtag cgaagtccaa aggcatgaaa ctgggggaag aaagttggct tgactgcgat
     4021 gatctgcgtg tagagctctg cgggccggga catggctggt gtgcccgaca acaggatcac
     4081 cctcttggca acctttagga ccggcatagc tgctcgacag cgggcagtcc tactgttttt
     4141 gaggaagtga gattcatcaa tgatgacaac tttaaaaggg gtttttagct gtttttccaa
     4201 cttgctaaga aggtcaaagc tgacaatgtt gatcaggcca gctgtcaggc ggtccttccc
     4261 agtcaccacg acgttgatgc aatctgggct cagagatggc agccaccgaa ggaaggcctg
     4321 ctcccaggtg aagcgcacgg aggatggcac caccaccagg agcggccact ccttccggta
     4381 aaaggctgcg atgcagatgg cttggatggt cttccccagg cccatgtcgt cagcgagcag
     4441 caggcggcct cctttggcta tggcaaaatt gactccagct ctctgaaagg gcatcagatt
     4501 agacacgagc ttggggtcca cttcagaaag gtctgcctct gggacatctg gcgtgagact
     4561 gagagatgtc ttcttgagct gagaagcaaa cgccagggtg agagtcgtgg gcagagggtc
     4621 cagctgaact tgtgggaggc agcgcacctt tgcaattagt ttactgtgct cttccaagag
     4681 aaagctccac ttcctggtct tgacatcata tcttctggaa tccatctgtt taaaaagcgc
     4741 aataaggtcc tgtgaataac tgatgtctgc ctcgaagtag gccctggaga tgagcatgca
     4801 tcgccctttg acaaatgaaa gggatggagc tgatggaagg ccggcctgtc cctcactgct
     4861 ggtggagggt gactcgctgc tgccataggc ccattccaga ggctgcaggt tgaccgtggg
     4921 gaggctctgg gccgctttca tcagggcact atagtcattc atgctgaagt tccacgtctt
     4981 ggtgtcagga tcataattct tgctgggcag ggtcttaaac actgcaatga gttctgcatt
     5041 gtacccaatc aacacctgga aacgatcgcc gttccttacg cactttccct tctgagagtt
     5101 cactcctttt tggactgagg accctgactt ctgctggagt cttccttctg tcctgggcgt
     5161 tacactctct gagctagaat ggatgtaaga aatgttctgc cccgaagggg aggcttttgc
     5221 tgtcttggcc tctaacttag catccctggg aggctgtcca gaggaatgag ctggtgtctc
     5281 ttgggaactc tttggtttgg ccagaggctt atgagttggg ttagcaaagg gtgtgaacct
     5341 gatctcaggt gaagcctgag catgaccttg acctaactca taactcaaga gctgttgttt
     5401 agggacctct ggaggacttt gtgccaaggg aggagagatt ccagtgagag ccatttgact
     5461 acgtggactg tggcctgggc aggctgtggg catttcttct ggctttttcc atattccctt
     5521 tgcctgaaaa ctgtgggaat catgaggtct ttggtcagca ttagatgagc tactgagatt
     5581 ctgttgcttg aaaatgacac catggctcac tggcttacaa gactcccttg ggaaattttg
     5641 ggatgggcct tgcttggcct ggaatgggtt gccagcaatg gaggtgcccg agctagtcct
     5701 ctgatgctgt tctgccaata acttctcagc tctgcgggcc agagcctttt gtcgattctc
     5761 ttcaatcttt ttcctctgct cctctgtaag aggcaaggac atgctaccgc taccgctagc
     5821 cttgtcgtca tcgtctttgt agtcgatgtc atgatcttta taatcaccgt catggtcttt
     5881 gtagtccatg cggccgctca cgacacctga aatggaagaa aaaaactttg aaccactgtc
     5941 tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac
     6001 atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc
     6061 gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg
     6121 aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga
     6181 aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc
     6241 cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca cgcaactggt
     6301 gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc
     6361 accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc
     6421 aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc
     6481 ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact atcttgccag
     6541 aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca
     6601 gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag
     6661 gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga actctcccac
     6721 ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt ggaagtaatt
     6781 caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc acacacggca
     6841 cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca
     6901 cgggggtacg gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt
     6961 tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc
     7021 aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagcctcac
     7081 gctagagcgg gcccggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa
     7141 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
     7201 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt
     7261 ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat
     7321 cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg
     7381 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     7441 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     7501 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     7561 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     7621 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     7681 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     7741 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     7801 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     7861 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     7921 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     7981 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     8041 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     8101 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag
     8161 atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     8221 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     8281 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     8341 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     8401 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     8461 gccttaaccg aattttttcc catcgcgatc taattcttcc ccgcttaata ctgacgctct
     8521 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     8581 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     8641 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     8701 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     8761 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     8821 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg
     8881 cgctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     8941 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     9001 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     9061 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     9121 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     9181 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     9241 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     9301 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     9361 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     9421 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     9481 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     9541 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     9601 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     9661 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     9721 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9781 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9901 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9961 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10021 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    10081 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10141 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10201 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10261 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10321 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10381 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10441 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10501 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10561 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10621 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10681 taagcattgg taactgtcag accaagttta ctcatatata c
//
