LOCUS       sequence-494367-       10742 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  Empty lentiviral backbone for generation of ClonMapper Duo Red 
            (H2B-mCherry) gRNA barcode library for use with polyA capture or 
            gRNA capture scRNA-seq technologies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10742)
  TITLE     Mapping cell-cell fusion at single-cell resolution
REFERENCE   2  (bases 1 to 10742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CMDuoRed_Crop-Seq-H2B-mCherry-WPRE-TS-hU6-BsmbI
FEATURES             Location/Qualifiers
     source          1..10742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      688..1276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1177..1196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1430..1447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1564..1585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1600..1605,1606..1623,1624..1630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1638..1654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1643..1665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1662..1678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1662..1678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1697..1717
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1699..1717
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1745..1971
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1972..2152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2199..2324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2817..3050
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3235..3279
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3460..3577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3716..4894
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3947..4885
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4842..4862
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4896..5273
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      5286..5295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5292..6002
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5437..5455)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5705..5724
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6013..6601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6066..6086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6484..6495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6794..7042
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6794..6814
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6965..6984
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8924..8999
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     LTR             9052..9232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9310..9431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9347..9366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9401..9420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9471..9606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9626..9645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9627..9645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9655..9672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9655..9671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9664..9686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9813..10268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9900..9919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10110..10131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10294..10398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10399..10467,10468..10742,1..517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10617..10636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
       61 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      121 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      181 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      241 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      301 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      361 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      421 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      481 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      541 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      601 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      661 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      721 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      781 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      841 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      901 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1021 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1081 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1141 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1201 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1261 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1321 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1381 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1441 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1501 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1561 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1621 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1681 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     1741 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     1801 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     1861 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     1921 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     1981 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2041 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2101 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2161 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2221 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2281 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2341 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2401 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2461 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     2521 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     2581 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     2641 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     2701 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     2761 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     2821 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     2881 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     2941 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     3001 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     3061 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3121 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3181 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3241 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3301 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3361 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     3421 tttcagaatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     3481 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3541 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     3601 agatccactt tggcgccggc tcgagggggc ccgggtgcaa agatggataa agttttaaac
     3661 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     3721 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     3781 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     3841 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     3901 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     3961 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     4021 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     4081 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     4141 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     4201 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     4261 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     4321 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     4381 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     4441 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     4501 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     4561 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     4621 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     4681 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     4741 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     4801 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     4861 tcaaagtttt tttcttccat ttcaggtgtc gtgacatgcc agagccagcg aagtctgctc
     4921 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
     4981 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
     5041 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
     5101 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
     5161 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
     5221 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatccac
     5281 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     5341 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     5401 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     5461 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     5521 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     5581 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     5641 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     5701 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     5761 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     5821 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     5881 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     5941 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     6001 aattaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6061 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6121 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6181 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6241 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6301 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6361 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6421 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6481 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6541 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6601 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6661 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatttc accattatcg
     6721 tttcagaccc acctcccaac cccgagggga cccaagcagt ggtatcaacg cagagtacat
     6781 ggggggaccc agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     6841 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     6901 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     6961 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     7021 cttgtggaaa ggacgaaaca ccggagacgg ttgtaaatga gcacacaaaa tacacatgct
     7081 aaaatattat attctatgac ctttataaaa tcaaccaaaa tcttcttttt aataacttta
     7141 gtatcaataa ttagaatttt tatgttcctt tttgcaaact tttaataaaa atgagcaaaa
     7201 taaaaaaacg ctagttttag taactcgcgt tgttttcttc acctttaata atagctactc
     7261 caccacttgt tcctaagcgg tcagctcctg cttcaatcat tttttgagca tcttcaaatg
     7321 ttctaactcc accagctgct ttaactaaag cattgtcttt aacaactgac ttcattagtt
     7381 taacatcttc aaatgttgca cctgattttg aaaatcctgt tgatgtttta acaaattcta
     7441 atccagcttc aacagctatt tcacaagctt tcatgatttc ttcttttgtt aataaacaat
     7501 tttccataat acatttaaca acatgtgatc cagctgcttt ttttacagct ttcatgtctt
     7561 ctaaaactaa ttcataattt ttgtctttta atgcaccaat atttaatacc atatcaattt
     7621 ctgttgcacc atctttaatt gcttcagaaa cttcgaatgc ttttgtagct gttgtgcatg
     7681 cacctagagg aaaacctaca acatttgtta ttcctacatt tgtgcctttt aataattctt
     7741 tacaatagct tgttcaatat gaattaacac aaactgttgc aaaatcaaat tcaattgctt
     7801 catcacataa ttgtttaatt tcagctttcg tagcatcttg ttttaataat gtgtgatcta
     7861 tatatttgtt tagtttcatt ttttctccta tatattcatt tttaatttta attctttaat
     7921 aatttcgtct actttaactt tagcgttttg aacagattca ccaacaccta taaaataaat
     7981 ttttagttta ggttcagttc cacttgggcg aacagcaaat catgacttat cttctaaata
     8041 aaattttagt aagtcttgtc ctggcatatt atacattcca tcgatgtagt cttcaacatt
     8101 aacaacttta agtccagcaa tttgagttaa gggtgttgct ctcaatgatt tcattaatgg
     8161 ttcaattttt aatttctttt cttctggttt aaaattcaag tttaaagtga aagtgtaata
     8221 tgcacccatt tctttaaata aatcttctaa atagtctact aatgttttat tttgtttttt
     8281 ataaaatcaa gcagcctctg ctattaatat agaagcttgt attccatctt tatctctagc
     8341 tgagtcatca attacatatc cataactttc ttcataagca aaaacaaaat ttaatccgtt
     8401 atcttcttct ttagcaattt ctctacccat tcatttaaat ccagttaaag tttttacaat
     8461 attaactcca tatttttcat gagcgattct atcacccaaa tcacttgtta caaaacttga
     8521 atatagagcc ggattttttg gaatgctatt taagcgtttt agatttgata attttcaatc
     8581 aattaaaatt ggtcctgttt gatttccatc taatcttaca aaatgaccat catgttttat
     8641 tgccattcca aatctgtcag catctgggtc attcataata ataatatctg catcatgttt
     8701 aataccatat tcaagcggta tttttcatgc aggatcaaat tctggatttg gatttacaac
     8761 atttttaaat gtttcatctt caaatgcatg ctcttcaacc tcaataacgt tatatcctga
     8821 ttcacgtaat atttttgggg taaatttagt tcctgttcca ttaactgcgc taaaaataat
     8881 ttttaaatct tttttagctt cttgctcttt tttgtacgtc tctgttttag agctagaaat
     8941 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     9001 tttttaagct tggcgtaact agatcttgag acactgcttt ttgcttgtac tgggtctctc
     9061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     9241 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     9301 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9361 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9421 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     9481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9541 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9601 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     9661 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     9721 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     9781 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9841 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9901 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9961 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    10021 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    10081 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10141 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10201 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    10261 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    10321 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    10381 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    10441 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    10501 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    10561 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10621 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    10681 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    10741 at
//
